Objoctive To investigate the effects of quercetin and X-ray on collagen synthesis of cultured human keloid-derived fibroblast and the mechanism. Methods Collagen synthesis of cultured human keloid and normal fibroblas...Objoctive To investigate the effects of quercetin and X-ray on collagen synthesis of cultured human keloid-derived fibroblast and the mechanism. Methods Collagen synthesis of cultured human keloid and normal fibroblasts were detected by hydroxyproline colorimetric analysis. Immunocytochemical staining was used to investigate collagen I and III expression, mRNA expression of collagen Ⅰ and Ⅲ, and transforming growth factor （ TGF）-β1 were assayed by reverse transcription-polymerase chain reaction （RT-PCR） and real-time PCR. Results Quercetin inhibited the collagen synthesis of both keloid and normal fibroblasts in a dose-dependent man- ner. Immunocytochemical staining indicated that collagenⅠ and Ⅲ were down-regulated by quercetin and X-ray （P 〈 0.05 ）, particularly collagen I （ P 〈 0. 05 ）. mRNA expression of both collagen I and III in quercetin groups significantly decreased compared with that in control group （ P 〈 0. 05 ）, especially in the group treated with both quercetin and X-ray （ P 〈 0. 01 ）. mRNA level of TGF-[31 gene was down-regulated by quercertin （ P 〈 0. 05 ）. Conclusions Quercetin will probably be one of the new medicines which could effectively treat keloid. Quercetin combined with X-ray could reduce the dose of radiation.展开更多
Objective:To treat and prevent auricular keloid so as to imporve the cure rate and lower the rate of recurrence. Methods:Taking 156 sufferers of auriclar keloid at the orthopaedics department of the Southwest Hospital...Objective:To treat and prevent auricular keloid so as to imporve the cure rate and lower the rate of recurrence. Methods:Taking 156 sufferers of auriclar keloid at the orthopaedics department of the Southwest Hospital under the Third Military Medical University from June, 2008 to June, 2011 as the research subject, this research carried out retrospective analysis and summary of different surgical methods with the clinical data of subseque nt treatments, including medicament, radiotheropy, pressure, etc. Results:There was no auricular cartilage necrosis and deformation in the total of 156 cases. It was found that 134 cases were cured in a year of postoperative follow-up, getting the care rate to 85.9%. The treatment was effective on 20 cases, with the effective rate of 12.8%, while it was ineffective on 2 cases, with the ineffective rate being 1.3%. However, two cases showed tendency towards recurrence, which were treated effectively by non-surgical methods like local hormone injection. There was neither recurrence nor apparent hyperplasia of hypertrophic scars on the rest cases, with the auricle and the earlobe in good shape. Conclusion:By auricular keloid excision and stripping surgery, surgical suture tension was reduced. Glucocorticoid and radiotherapy were instantly applied to inhibit pyperplasia of cicatrical tissue. Subsequently, pressure, anti-scarring drugs and silicone membrane were adopted to ensure fine postoperative apperance and reduce recurrence. These methods were proved to be effectiveand they provided systematic and effective treatment for auricular keloid.展开更多
Objective： Keloids are exuberant cutaneous scars that form due to abnormal growth of fibrous tissue fol- lowing an injury. The primary aim of this study was to assess the efficacy and mechanism of hyperbaric oxygen t...Objective： Keloids are exuberant cutaneous scars that form due to abnormal growth of fibrous tissue fol- lowing an injury. The primary aim of this study was to assess the efficacy and mechanism of hyperbaric oxygen therapy （HBOT） to reduce the keloid recurrence rate after surgical excision and radiotherapy. Methods： （1） A total of 240 patients were randomly divided into two groups. Patients in the HBOT group （O group） received HBOT after surgical excision and radiotherapy. Patients in the other group were treated with only surgical excision and radiotherapy （K group）. （2） Scar tissue from recurrent patients was collected after a second operation. Hematoxylin and eosin （H＆E） staining was used to observe keloid morphology. Certain inflammatory factors （interleukin-6 （IL-6）, hypoxia-inducible factor-1α （HIF-1α）, tumor necrosis factor-α （TNF-α）, nuclear factor KB （NF-κB）, and vascular endothelial growth factor （VEGF）） were measured using immunohistochemical staining. Results： （1） The recurrence rate of the O group （5.97%） was significantly lower than that of the K group （14.15%）, P〈0.05. Moreover, patients in the O group reported greater satisfaction than those in the K group （P〈0.05）. （2） Compared with the recurrent scar tissue of the K group, the expression levels of the inflammatory factors were lower in the recurrent scar tissue of the O group. Conclusions： Adjunctive HBOT effectively reduces the keloid recurrence rate after surgical excision and radiotherapy by improving the oxygen level of the tissue and alleviating the inflammatory process.展开更多
文摘Objoctive To investigate the effects of quercetin and X-ray on collagen synthesis of cultured human keloid-derived fibroblast and the mechanism. Methods Collagen synthesis of cultured human keloid and normal fibroblasts were detected by hydroxyproline colorimetric analysis. Immunocytochemical staining was used to investigate collagen I and III expression, mRNA expression of collagen Ⅰ and Ⅲ, and transforming growth factor （ TGF）-β1 were assayed by reverse transcription-polymerase chain reaction （RT-PCR） and real-time PCR. Results Quercetin inhibited the collagen synthesis of both keloid and normal fibroblasts in a dose-dependent man- ner. Immunocytochemical staining indicated that collagenⅠ and Ⅲ were down-regulated by quercetin and X-ray （P 〈 0.05 ）, particularly collagen I （ P 〈 0. 05 ）. mRNA expression of both collagen I and III in quercetin groups significantly decreased compared with that in control group （ P 〈 0. 05 ）, especially in the group treated with both quercetin and X-ray （ P 〈 0. 01 ）. mRNA level of TGF-[31 gene was down-regulated by quercertin （ P 〈 0. 05 ）. Conclusions Quercetin will probably be one of the new medicines which could effectively treat keloid. Quercetin combined with X-ray could reduce the dose of radiation.
文摘Objective:To treat and prevent auricular keloid so as to imporve the cure rate and lower the rate of recurrence. Methods:Taking 156 sufferers of auriclar keloid at the orthopaedics department of the Southwest Hospital under the Third Military Medical University from June, 2008 to June, 2011 as the research subject, this research carried out retrospective analysis and summary of different surgical methods with the clinical data of subseque nt treatments, including medicament, radiotheropy, pressure, etc. Results:There was no auricular cartilage necrosis and deformation in the total of 156 cases. It was found that 134 cases were cured in a year of postoperative follow-up, getting the care rate to 85.9%. The treatment was effective on 20 cases, with the effective rate of 12.8%, while it was ineffective on 2 cases, with the ineffective rate being 1.3%. However, two cases showed tendency towards recurrence, which were treated effectively by non-surgical methods like local hormone injection. There was neither recurrence nor apparent hyperplasia of hypertrophic scars on the rest cases, with the auricle and the earlobe in good shape. Conclusion:By auricular keloid excision and stripping surgery, surgical suture tension was reduced. Glucocorticoid and radiotherapy were instantly applied to inhibit pyperplasia of cicatrical tissue. Subsequently, pressure, anti-scarring drugs and silicone membrane were adopted to ensure fine postoperative apperance and reduce recurrence. These methods were proved to be effectiveand they provided systematic and effective treatment for auricular keloid.
基金Project supported by the National Natural Science Foundation of China(No.81471885)the Beijing Natural Science Foundation(No.7172172),China
文摘Objective： Keloids are exuberant cutaneous scars that form due to abnormal growth of fibrous tissue fol- lowing an injury. The primary aim of this study was to assess the efficacy and mechanism of hyperbaric oxygen therapy （HBOT） to reduce the keloid recurrence rate after surgical excision and radiotherapy. Methods： （1） A total of 240 patients were randomly divided into two groups. Patients in the HBOT group （O group） received HBOT after surgical excision and radiotherapy. Patients in the other group were treated with only surgical excision and radiotherapy （K group）. （2） Scar tissue from recurrent patients was collected after a second operation. Hematoxylin and eosin （H＆E） staining was used to observe keloid morphology. Certain inflammatory factors （interleukin-6 （IL-6）, hypoxia-inducible factor-1α （HIF-1α）, tumor necrosis factor-α （TNF-α）, nuclear factor KB （NF-κB）, and vascular endothelial growth factor （VEGF）） were measured using immunohistochemical staining. Results： （1） The recurrence rate of the O group （5.97%） was significantly lower than that of the K group （14.15%）, P〈0.05. Moreover, patients in the O group reported greater satisfaction than those in the K group （P〈0.05）. （2） Compared with the recurrent scar tissue of the K group, the expression levels of the inflammatory factors were lower in the recurrent scar tissue of the O group. Conclusions： Adjunctive HBOT effectively reduces the keloid recurrence rate after surgical excision and radiotherapy by improving the oxygen level of the tissue and alleviating the inflammatory process.
