“种养+”实践活动形式探索

在2022年版科学课程标准指导下,明确科学核心素养的目标内涵,认识到学生在科学课堂学习中的实践缺失,基于不同主题项目、多种实践形式开展小学科学“种养+”实践活动的探索,最终实现学生科学素养的落地发展。

生态种养新模式:菜—猪—沼

杭州市余杭区兴旺疏果有限公司，在当地农业等部门的指导下，大胆创新，按照农业生态原理及“减量化、资源化、无害化、生态化”的畜禽污染治理原则，改革传统的种养结合模式，尝试在大棚内种菜养猪(羊)，并以沼气池为纽带，促进种植和养殖的有机结合，实现了经济、生态、社会效益的最优化，走上了“种养沼”良性循环的发展道路，获得了很大的成功。这一生态模式为我省首例。

Key Techniques of an Ecological Pattern“Planting Rice in One Season and Breeding Red Swamp Crawfish in Three Seasons” for Green Production in Lixiahe Region of Jiangsu Province

Three big field projects, independent irrigation and drainage facilities, and a blocking net, which are auxiliary projects of a green planting and breeding pattern composed of rice and red swamp crawfish （Procambarus clarkii）, spatial and temporal coupling technology of ＂planting rice in one season and breeding red swamp crawfish in three seasons＂, green fertilization technology, green prevention and control technology, control technology of water level, and throwing technology of bait in Lixiahe region of Jiangsu Province were introduced successively, which can provide technical support for the development of ecological planting and breeding patterns and realization of green production in paddy fields.

生态农业模式与节能型家庭农场的构建

家庭农场是新疆农业发展的一个趋势,家庭农场生产什么,如何生产,如何解决家人和员工的日常生活问题都涉及到家庭农场的模式问题。根据新疆的特点,尤其是南疆的特点提出新疆应建立生态节能型家庭农场模式。生态节能型家庭农场模式,包括两部分内容:生产区的"种养结合"生态农业模式和生活区的"四位一体"节能型模式。

Inoculation Effects of Dendrobium officinale Mycorrhizal Fungi on Their Plantlets

[Objective] This study aimed to explore the inoculation effects of Dendrobium officinale mycorrhizal fungi on their plantlets. [Method] Endophytic strains Tj1, Tj2 and Tj3 were obtained by isolation and purification from mycorrhiza of wild Dendrobium officinale and inoculated on the root system of Dendrobium officinale for inoculation test. [Result] Under tissue-culture conditions, at early stage, Tj1 strain hadn＇t shown promotion effect on Dendrobium officinale, Tj2 strain had shown relatively strong promotion effects, and Tj3 strain had promoted the growth of roots; at late stage, Tj1 strain had shown relatively strong promotion effects, Tj2 strain had shown the best inoculation effects and the strongest promotion effects, while Tj3 strain had caused root and seedling rot problems of the plantlets; under outdoor conditions, after inoculation with Tj2 strain, the number of leaves and lateral buds were increased, the growth of lateral root and the increase of plant height were significant, the leaves of Dendrobium officinale plantlets were large and dark green and an obvious root enlargement phenomenon was observed. [Conclusion] The two inoculation methods both indicate that Tj2 strain has relatively strong promotion effects on the growth of Dendrobium officinale roots and shoots, the increase of plant number and plant height, and the germination of new shoots and roots, which proved the effective establishment of symbiotic relationship between Tj2 strain and Dendrobium officinale. Therefore, T2 strain has practical application values on the successful cultivation of Dendrobium officinale plantlets.

Varietal Characteristics and Nutrition Characteristics of Several New Varieties of High Biomass Sugarcanes for Sugar and Energy

[Objective] The aim was to study the varietal characteristics of six new varieties of high biomass sugarcanes for sugar and energy namely GT02-833,GT97-69,GT02-351,GT03-2287,B9 and GT02-770,as well as the nutrition characteristics of them under drip irrigation conditions. [Method] Industrial and agronomic characters,total N,total P,total K and chlorophyll content were determined. [Result] Cane yield,total biomass yield and sugar content of six varieties were more than 180,200 and 25 t/hm2 respectively,which were higher than that of the control Xintaitang 22. The total nitrogen and total phosphorus contents of six varieties were high and persistent from Jul to Nov,but they decreased sharply in Dec. Total potassium content of all varieties increased from Jul to Dec. Chlorophyll content of all varieties were high from Aug to Oct,but they decreased sharply in Nov. Total nitrogen,total phosphorus and total potassium content of GT02-833,GT97-69 and B9 were higher than that of CK. [Conclusion] GT02-833,GT97-69 and B9 had characteristic of efficient use of nutrients,thus should be extended as elite varieties.

Callus Induction and Plant Regeneration from Mature Seeds of Perennial Ryegrass

Objective The aim was to explore callus induction and plant regeneration of perennial ryegrass, as well as provide the foundation for transgenic research on perennial ryegrass.[ Methed] Mature seeds of perennial ryegrass were used as explants to study the effects of different hormone compositions on callus induction, proliferation and plant differentiation. Result The result showed that the induction rate achieved its highest on 2,4-D of 8 mg/L combining with 6-BA of 0.025 mg/L, which was up to 56.42%. Callus were differentiated after two to three generations, the highest differentiation rate 34.14% was achieved in the medium contained MS medium with 6-BA of 2 mg/L, and the differentiation rate was obviously affected by the callus condition after proliferation. The root inducing medium, containing 0.5 mg/L NAA and MS medium with half of macroelement, gained 98% root inducing rate. Conclusien A high frequency genetic regeneration system was established.

Security Risk Analysis on the Nutrients and Heavy Metals of Biogas Slurry in Agricultural Application

[Objective] This study was to provide scientific basis for the security application of biogas slurry in agriculture through the analysis on the security risk of nu- trients and heavy metals of biogas slurry in agricultural application. [Method] The components of the slurry produced by the anaerobic digestion of straws and manures were systematically analyzed. Different types of biogas slurry with different concentrations were used to conduct the germination test. [Result] The types and proportions of raw materials, and alkali pretreatment affected the compositions and nature of the biogas slurry. Alkali pretreatment could reduce the contents of heavy metals and improved the contents of nitrogen, phosphorous, organic matter and available components. The contents of heavy metals in the biogas slurry were as follows： Cd of 8.76 μg/kg, Cr of 52μg/kg, Pb of 210 μg/kg, Hg of 0.512 μg/kg, As of 140μg/kg, and the contents of Cu and Zn were the highest among the heavy metals, respectively, 279 and 680 μg/kg. All the contents were under the limit standards of the urban wastes for agricultural use. The germination tests of the seeds verified that the slurry with the concentration of lower than 5% could accelerate the germination, while the salt stress could inhibit the growth of seedlings in the slurry. [Conclusion] When the dung are uses as the raw materials for fermentation, appropriate control should be conducted on the feeding proportion or the heavy metals should be pre-removed; when the slurry is used as the liquid fertilizer to apply in the farmland, the dilution or desalting treatment should be conducted at first.

A New Model of Recycling Agricultural Production

[Objective] The aim was to research production model of recycling agricul- ture. [Method] The production model of recycling agriculture integrating planting and breeding was explored on basis of pig raising, and maize and peanut planting, with consideration of existing problems of planting and breeding integration in China. [Result] Ecological and economic benefits of pig farms and planting bases increased significantly, and all indices improved in different degrees. [Conclusion] The research provides valuable references for pig raising in China.

Differences in Primary Callus Induction among Different Anthurium andraeanum Varieties

[Objective] This research aimed to optimize continuously the highly efficient regeneration system of Anthurium andraeanum. [Method] The leaves and petioles of four A. andraeanum varieties were used as explants to investigate the differences in primary callus induction among different A. andraeanum varieties. [Result]The callus formation capacity of SAM and SST was stronger than that of SDM and SHG. Among the four varieties, the leaf regeneration capacity of SAM, SDM and SHG was stronger than the corresponding petiole regeneration capacity. However,the petiole regeneration capacity of SST was stronger. The optimum medium for petiole callus induction of SST was 1/2 MS ＋ TDZ 4.0 mg/L ＋ 2, 4-D 0.2 mg/L with induction rate of 87.5%; the optimum medium for leaf callus induction of SAM was 1/2 MS ＋ TDZ 2.0 mg/L ＋ 2, 4-D 0.2 mg/L with induction rate more than90%; the optimum medium for leaf callus induction of SDM and SHG was all 1/2MS ＋ ZT 2.0 mg/L ＋ 2, 4-D 0.2 mg/L with induction rates of 59.34% and 79.63%,respectively. [Conclusion] In addition to variety differences, the differences in differentiation ability among different types of calluses should be also taken into account in the establishment and optimization of tissue culture and rapid propagation technology system of A. andraeanum.

Study on the Changes of Physiological Indexes of Water Spinach in Eutrophication Water

[Objective] Effect of hypoxic water culture on physiological characteristic of water spinach and its hypoxia tolerance were studied.[Method] Water spinach was planted in soil and eutrophication water by means of floating bed,respectively,and the change of physiological indexes including chlorophyll,proline（Pro）,malondialdehyde（MDA）,soluble sugar and soluble protein in water spinach leaves at mature stage was researched,while the hypoxia tolerance of water spinach and the effect of plant density on water spinach growth were discussed in our paper.[Result] In the hypoxic eutrophication water,the content of total chlorophyll,malondialdehyde,soluble sugar and soluble protein in water spinach leaves was lower than that of soil culture,with higher proline content,which showed that water spinach had better tolerance to hypoxic eutrophication water;the higher the plant density,the lower the chlorophyll content in water spinach leaves,and there was no significant effect of plant density on proline and malondialdehyde content,while soluble protein content was higher under high plant density.[Conclusion] The best plant density of water spinach was 66 plants per floating bed with the area of 2 m2,which could provide theoretical basis for the application of water spinach in floating bed.

Balance of Manure and Farmland Load in a Breeding Farm

[Objective] The aim was to research balance between manure of breeding farm and farmland load. [Method] N balance of farmlands after manure fertilized was researched in a hoggery in Beijing （600 base sows and 647 breeding pigs）. [Result] The suitable N input in neighboring farmlands was 191.9 kg/（hm 2 ·y） and the farmland area satisfying balance between growing and breeding was 53.7 hm 2 in the hoggery. [Conclusion] The research provided a scientific method for balance between growing and breeding.

Study on Germplasm Resources of Tartary Buckwheat (Fagopyrum tataricum) in Shanxi Province

This study analyzed the tartary buckwheat recorded in the books Catalogue of Crop Germplasm Resources in Shanxi Province and Catalogue of Chinese Buckwheat Germplasm Resources by Excel 2003 and DPS 7.5 from the aspects of distribution areas,main agronomic traits and nutritional components,so as to lay a foundation for the selection of excellent buckwheat germplasm resources and improvement of buckwheat varieties.The results showed that different buckwheat varieties vary in plant height,node number of the main stem,grain weight per plant,grain weight per plant and kilo-grain weight.Compared with the national average values,buckwheat in Shanxi is higher in protein and fat,but lower in vitamin E and vitamin PP;it is rich in Ca,Mg,Cu,P,Se,Fe,Mn and Zn;and it contains 18 amino acids with the content of glutamic acid being the highest and the content of tryptophane being the lowest.After analyzing and classifying the tartary buckwheat germplasm resources recorded in Catalogue of Grop Germplasm Resources in Shanxi Province and Catalogue of Chinese Buckwheat Germplasm Resources,breeders could breed new varieties with high yield and quality and strong stress resistance by crossbreeding with these germplasm resources.

Quantitative Allocation Analysis of "Raising by Planting" System Combining Fruits and Poultry for Mountainous Region in Headwaters of Dongjiang River

[Objective] The aim was to promote nutrient cycling and utilization in the mountain＇s system combining fruits and poultry and to control non-point source pollution produced from swine raising and navel orange planting in headwaters of Dongjiang River. [Method] The quantitative analysis was mainly conducted for the so called ＂raising by planting＂ which is about material cycle in ＂pig-methane-fruit-fish＂ model and energy cascade utilization, based on relationship between excreted amount by livestock and the utilized quantity in Xinlin Farm in Longtang Town, Dingnan County, Jiangxi Province. [Result] Considering N requirement by fruit trees, a navel orange orchard （1 mu） could support about 2.72 pigs, the equipped biogas pool （1.88 m3） could support 1 166.67 kg of duckweeds and a fish pond could support 25.57 grass carps. In contrast, a satsuma orchard （1 mu） could support about 1.96 pigs, the equipped biogas pool （1.35 m3） could support 841.53 kg duckweeds and the fish pond could support 18.44 grass carps. [Conclusion] The results provided scientific references for quantitative allocation of members in ＂pig-methane-fruit-fish＂ model when popularized in headwaters of Dongjiang River.

Analysis on the Distribution of Main Physicochemical Parameters of Color Wheat and Study on its Layering Milling Technology

[Objective] The aim was to promote color wheat industrialization and meet the demand of people on nutritious grain, making both natural and safe food possible. [Method] Hardness indices of wheat were measured and layering peeling and milling technology was adopted to explore nutrients distribution in color wheat and effect of hardness on milling of wheat layers. [Result] The results indicated that total content of amino acid in color wheat was higher than that of common wheat by 13.91%-23.32%; Zhongpu Black 1 and Zhongpu Green 1 exceeded common wheat in Zn, Fe and Ca, but Zhongpu Purple 1 was generally lower; Zhongpu Green 1 was significantly higher in Fe and Ca by 371.80% and 102.86%, respectively. Mean- while, it was found that nutrients distribution of color wheat was similar to that of common ,one, namely, pericarp, testa, aleurone layer and embryo were abundant with nutrients. In addition, color wheat was concluded nutritious one and milling in- dustrialization of wheat layers could be achieved through layer-milling and separation technology. Furthermore, wheat hardness was proved the key element influencing milling of wheat layers. [Conclusion] The research set an example for nutrition development and utilization of color wheat.

Genetic Differences and Selection of Major Nutritional Quality Characters in Millet

[Objective] The aim was to enhance specificity and efficiency of quality breeding of millet. [Method] Difference analysis, correlation analysis, principal compo- nent analysis, and path analysis were conducted on nutritional quality characters of 29 millet varieties. [Result] Variance coefficients of nutritional quality characters among different varieties were in descending order of crude protein 〉 crude fiber 〉 fat 〉 water 〉 total carbohydrate. The results of correlation analysis suggested that crude protein exhibited extremely significant negative correlation with total carbohydrate （correlation coefficient of -0.822 4） and significantly negative correlation with fat （correlation coefficient of -0.380 7）; the rest characters showed no significant correla- tion. In addition, according to PCA and path analysis, four characters, including crude protein, fat, total carbohydrate and crude fiber, determined 97.35% of variance of nutritional quality and could be taken as references for selection of breeding ma- terials, with a determination coefficient of 1.000 0. Fat and carbohydrate were typical nutritional quality characters of breeding materials, which should be selected in low generation. However, crude protein and crude fiber should be selected for millet in high generation. [Conclusion] The research provides references for breeding of new millet varieties with high nutritional quality.

Growth of the Crystals of Nitrogenase MnFe Protein

Under a suitable condition of crystallization, dark brown short rhombohedron crystals could be obtained from nitrogenase MnFe protein purified from a mutant UW3 of Azotobacter vinelandii Lipmann grown in Mn-containing but Mo- and NH3-free medium. The possibility of crystallization, and number, size and quality of crystals were obviously dependent on concentrations of NaCl, MgCl2, PEG 8000,Tris and Hepes buffer and on methods for crystallization. PEG concentration affected on the shape of the crystals. The optimal, concentrations of the chemicals for crystallization of MnFe protein were slightly different from those for crystallization of Delta nifZ MoFe protein from a nifZ deleted strain of Azotobacter vinelandii. SDS-PAGE showed that the protein from the dissolved crystals was almost the same as MnFe protein before crystallization, indicating that the crystal was formed from MnFe protein.

Optimization of the Conditions for Using Sewage to Cultivate Navicula tenera

[ Objective] This study was to improve the biomass of Navicula tenera, and thus to provide reference for achieving the industrial production of Navicula tenera. [Method] The feasibility of using sewage to cultivate Navicula tenera was preliminarily investigated based on the consideration of regional characteristics; and a series of culture conditions including the nutrient source of nitrogen（N）, phosphorus（P）, iron（Fe）, silicon（ Si ） and the salinity in medium for culturing Navicula tenera, were optimized by single factor test and orthogonal design.[Result] The optimized conditions for cultivating Navicula tenera using sewage are as follows： the water from Xiaoerlou Artificial Lake of Nanjing University of Technology as basic solvent; 360 mg/L urea; 150 mg/L N2HPO4 · 12H2O; 50 mg/L ferric citrate; 2 000 mg/L Na2SiO3 · 9H2O; 2.0 mol/L salinity. Navicula tenera was strongly adaptive to sewage and could well uptake the nutrient sources in the sewage. Under the optimized conditions, the culture cost decreased, and meanwhile the biomass of Navicula tenera reached 4. 766 g/L which is 3.57 multiples over original medium and 1.9 multiples over optimized medium No. 1.[ Conclusion] This study laid a foundation for the combination of culturing Navicula tenera in large scale and sewage treatment.

Analysis of genetic diversity for wild and captive green peafowl populations by random amplified polymorphic DNA technique

The genetic diversity of the populations for 14 wild green peafowls (Pavo muticus) and 18 captive green pea-fowls was investigated by using the technology of random amplified polymorphic DNA (RAPD). Totally 161 and 166 ampli-fied bands were obtained by using 23 arbitrary primers to amplify the genomic DNA of wild and captive green peafowls re-spectively. The results showed that the average relative genetic distance of the wild and captive green peafowls popula-tions was 0.0555 and 0.1355, respectively, and difference of the average relative genetic distances between the two popu-lations was 0.1635. The Shannon diversity index for the wild and captive green peafowl populations was 0.4348 and 1.0163, respectively, which means that there exists significant difference in genetic diversity between the two populations, and the genetic diversity of wild green peafowl was low. The two populations originated from two different families according to analysis by the UPGMA method. This research can provide the theoretical basis for supervising genealogies management of peafowl populations.

Interspecific Cross Compatibility of Rhododendron in Changbai Mountain

In order to explore the interspecific cross compatibility of Rhododendron and the feasibility of interspecific cross in germplasrn innovation, reciprocal crosses among five species of Rhododendron in Changbai Mountain were made, In addition, the hybrid embryos were cultured in vitro. The results showed that the species of Rhododendron in Changbai Mountain had significant differences in reciprocal cross compatibility. When Rh. chrysanthum Pall. was used as female parent, it showed high cross compatibilities with Rh. mucronulatum Turcz. （92.55%）, Rh. mucronulamm Turcz. f, album Nakai （94.14%） and Yabuli Rhododendron （92.42%）, and no signifi- cant differences were found in fruit setting rate by serf-poUination. The germination rates of hybrid embryos were 13.60%, 11.59% and 4.12%, respectively. The hybrid seedlings had weak growth vigor and albino status and were trended to be aborted. After 2-3 generations of subculture, the growth vigor of hybrid seedlings were re- covered gradually, and their differentiation rate was also significantly higher than that of Rh. chrysanthum Pall. seedlings. But the hybrid seedlings were still yellowish green. In reciprocal crosses, the fruit setting rates were significantly reduced. The combination with the highest fruit setting rate （36.51%） was Yabuli Rhododendron x Rh chrysanthum Pall The fruit setting rates of reciprocal crosses between Rh chrysan- thum Pall and Rh. mucronulatum Turcz. and Rh.： chrysanthum Pall. and Rh. schlip- penbachii Maxim were 6.52% and 0, respectively. However, the hybrid embryo＇s germination rate （39,81%） of Yabuli Rhododendron x Rh. chrysanthum Pall. was significantly higher than that of their original cross. Moreover, the hybrid seedlings grew strongly and could be directly transplanted without subculture.