Objective: To purify and identify the osteoclasts from the tissue of humangiant cell tumor of bone. Methods: We have developed a new method that allows the purification oflarge numbers of authentic osteoclasts (OCs). ...Objective: To purify and identify the osteoclasts from the tissue of humangiant cell tumor of bone. Methods: We have developed a new method that allows the purification oflarge numbers of authentic osteoclasts (OCs). The OCs were isolated from tissue of human giant celltumor of bone by 0.25% trypsin and collagenase. We characterized OCs in terms of the expression ofdifferent phenotypic markers of OCs. The phenotypic markers of OC included Tartrate-resistant acidphosphatase staining (TRAP). The expression of calcitonin receptor (CTR), cathepsin K and receptoractivator of necrosis factor κB (RANK) mRNA were examined by RT-PCR. Results: The OC cell purifiedby above method functioned normally in vitro. The purity was about 79.7%. They showed the normalosteoclast phenotypes markers of OC. Conclusion: The method provides a system for performingbiochemical and molecular studies of OCs. The study indicates that the method of purifying theosteoclasts from human GCT cell can be used for research of bone metabolism.展开更多
The constrained Viterbi algorithm （C-VA） makes use of some prior reliable information to reduce complexity and improve performance of Viterbi algorithm （VA）. However it can only be used in the concatenate code sch...The constrained Viterbi algorithm （C-VA） makes use of some prior reliable information to reduce complexity and improve performance of Viterbi algorithm （VA）. However it can only be used in the concatenate code scheme because the detection result of cyclic redundancy check code （CRC） is exploited to provide reliable information. In this paper, a different way is proposed to provide reliable information for C-VA, which is possible to be used in simple codec. Known bits were added to the set partitioning in hierarchical trees （SPIHT） coded image sequence periodically and directly. They were thought to be reliable information for C-VA in the decoder. Experimental results show that the proposed scheme can obtain much better error resilient ability compared with conventional VA under the extremely inferior channel condition if the best desired quality of reconstructed images can be sacrificed.展开更多
文摘Objective: To purify and identify the osteoclasts from the tissue of humangiant cell tumor of bone. Methods: We have developed a new method that allows the purification oflarge numbers of authentic osteoclasts (OCs). The OCs were isolated from tissue of human giant celltumor of bone by 0.25% trypsin and collagenase. We characterized OCs in terms of the expression ofdifferent phenotypic markers of OCs. The phenotypic markers of OC included Tartrate-resistant acidphosphatase staining (TRAP). The expression of calcitonin receptor (CTR), cathepsin K and receptoractivator of necrosis factor κB (RANK) mRNA were examined by RT-PCR. Results: The OC cell purifiedby above method functioned normally in vitro. The purity was about 79.7%. They showed the normalosteoclast phenotypes markers of OC. Conclusion: The method provides a system for performingbiochemical and molecular studies of OCs. The study indicates that the method of purifying theosteoclasts from human GCT cell can be used for research of bone metabolism.
基金Project supported by the National Natural Science Foundation of China (Grant No.60372070)
文摘The constrained Viterbi algorithm （C-VA） makes use of some prior reliable information to reduce complexity and improve performance of Viterbi algorithm （VA）. However it can only be used in the concatenate code scheme because the detection result of cyclic redundancy check code （CRC） is exploited to provide reliable information. In this paper, a different way is proposed to provide reliable information for C-VA, which is possible to be used in simple codec. Known bits were added to the set partitioning in hierarchical trees （SPIHT） coded image sequence periodically and directly. They were thought to be reliable information for C-VA in the decoder. Experimental results show that the proposed scheme can obtain much better error resilient ability compared with conventional VA under the extremely inferior channel condition if the best desired quality of reconstructed images can be sacrificed.
