江苏小剧场昆曲的非典型路径研究

学界一直未明确为“小剧场戏曲”定义。因其命名导引自小剧场话剧,小剧场话剧的某些特征自然被纳入对小剧场戏曲的认定与考量,实验、先锋这些标志性的特质也成为小剧场戏曲认证的重要标尺。但小剧场戏曲毕竟还是戏曲,戏曲自身的传统特征也应被纳入定义的范畴。于是,先锋和传统就成为小剧场戏曲可以依循的两条路径。当下的小剧场创作大多走的是一条中间路径,在保留传统戏曲规范的基础上嵌入先锋的理念和形式,江苏昆曲却在最先锋和最传统的路径选择中展开了“素昆”和新编折子戏的非典型探索,并在长期的实践中形成具有可操作性和可复制性的审美经验和规范。

Optimization of V_A and V_E Production by a Paecilomyces marquandii Strain

In order to further improve the yield of VA and VE produced by a Paecilomyces marquandii strain, using PDA liquid medium as the basic medium, the effects of adding nitrogen source, pH value and Fe2＋ on the VA and VE production were compared with an orthogonal experiment. The results showed that the VA yield was significantly increased when sodium nitrate was added as nitrogen source, pH value of medium was 6, but Fe2＋ was not added; the addition of peptone into the basic medium as a nitrogen source contributed to the improvement of VE yield in mycelia; the PDA liquid medium, as a basic medium, was more conducive to the VA production by the Paecilomyces marquandii strain.

Pesticidal character of phytoecdysteroids from Ajuga multiflora Bunge (Labiatae) on larvae of Cryptorrhynchus lapathi L. (Coleoptera: Curculionidae)

Eight kinds of phytoecdysteroids extracted from different parts of Ajuga multiflora Bunge (Labiatae) that were collected from different places at different time were tested for killing effects on the 2-instar larvae of Cryptorrhynchus lapathi L by adding them to the artificial diet of larvae. The experimental results indicated that adding 1-3-mL phytoecdysteroids to the artificial diet could lead 58%-100% of 2-instar larvae of C. lapathi to death within 24 days. The phytoecdysteroids extracted from the whole plant of A. multiflora which was collected before flowering time were much more effective than those extracted from the plants collected at flowering and after flowering periods, and the modified mortality rate of larvae reached 65.22%, 85.07%, and 98.11% at the dosage level of 1-mL, 2-mL, and 3-mL extracts, respectively. The extract made from root of A. multiflora plant was more effective in killing efficiency than those from stem and leaves, and the average death rates of larvae were up to 100%, 98.20% and 98.32% at dosage levels of 1-mL, 2-mL, and 3-mL extracts, respectively. The killing speed of the extracted phytoecdysteroids was slower than that of triflumuron, hexaflumuron or deltamethrin emulsifiable concentrate. The mortality rate of larvae is closely related to the feeding duration on the diets containing phytoecdysteroids. Feeding on the diets with addition of phytoecdysteroids for 16 days, more than 80% of treated 2-instar larvae of C. lapathi were led to death. The killing effect of the extracts was little affected by the growth areas of A. multiflora plant and the adding way to artificial diet.

The extraction of pigments from fresh Laminaria japonica

The pigments in Laminaria japonica was extracted with six organic solvents and analyzed in spectroscopy analysis. The extractions conditions were screened by an orthogonal test and the quantity of extracted pigments was determined spectroscopically. The results show that: (1) among the six organic solvents, acetone was the most effective one for the extraction; (2) the optimum extraction conditions were as follows: the ratio of S/M (solvent volume/material weight) was 30 ml/g; fresh seaweed was extracted 2 times in 2h; (3) the average total content of pigments was 1.85 mg/g (calculated with dry L. japonica).

Geochronology, petrogenesis and tectonic significance of Dahongliutan pluton in Western Kunlun orogenic belt, NW China

The Dahongliutan granitic pluton,in the eastern part of the West Kunlun orogenic belt,provides significant insights for studying the tectonic evolution of West Kunlun.This paper presents a systematic study of LA-ICP-MS zircon U Pb age,major and trace elements,Sr-Nd-Hf isotopes,and the first detailed Li isotope analysis of the Dahongliutan pluton.LA-ICP-MS zircon U Pb dating shows that the Dahongliutan granites were emplaced in the Late Triassic((213±2.1)Ma).Geochemical data show relatively high SiO2 contents(68.45 wt%73.62 wt%)and aluminum saturation index(A/CNK=1.111.21)indicates peraluminous high-K calc-alkaline granite.The Dahongliutan granites are relatively high in light rare earth elements(LREE)and large ion lithophile elements(LILEs)(e.g.,Rb,K,Th),and relatively depleted in high field strength elements(HFSEs)(e.g.,Nb,Ta,P,Ti).TheεNd(t)values range from 8.71 to 4.73,and(87Sr/86Sr)i=0.70870.71574.Zircons from the pluton yield 176Hf/177Hf values of 0.2826181 to 0.2827683,andεHf(t)values are around 0;the two-stage Hf model ages range from 0.974 to 1.307 Ga.Theδ7Li values are 0.76‰3.25‰,with an average of 2.53‰.Isotopic compositions of the pluton suggest a mixed trend between the partial melting of the Middle Proterozoic ancient crustal material and a juvenile mantle-derived material.This study infers that the Dahongliutan rock mass is formed in the post-collisional extension environment,when the collision between South Kunlun and the Tianshuihai terranes results in the closure of the Palaeo-Tethys.The mantle-derived magma results in partial melting of the lower crust.

Selective Mono-Ozonolysis of a Cyclic 1,5,9-Cyclododecatrien

Some unsaturated compounds with the required （Z,Z）-double bonds configuration are synthesized through controlled ozonolysis process of 1,5,9-cyclododecatriene by determining the necessary amount of ozone passing through to selectively guarantee the breakage of a double bond while leaving other two bonds as （Z,Z）. The products were diagnosed by using NMR spectrum, infrared （IR） and mass spectrometer techniques. It is concluded that these products could widely be used in the synthesis of insect pheromones instead of insecticides and as useful intermediate compounds in many chemical reactions.

Water Quality, Climatic and Plant Traits as Predictors on Abundance of Insects in Paddy Field, Tanjung Karang, Selangor, Malaysia

The abundance of beneficial insects in the paddy field can be influenced by the abundance of insect pests, water parameters, climatic factors and plant traits. This study was conducted to determine effects of water parameters, climatic factors and plant traits on abundant insects. Findings of the studies showed that there were significant correlations between all beneficial insects in paddy field and water parameters tested, namely, water temperature, salinity, turbidity, pH, dissolved oxygen, biological oxygen demand, chemical oxygen demand, ammonia and total suspended solids. Moreover, the selected water parameters of water temperature, dissolved oxygen, biological oxygen demand and chemical oxygen demand were good predictors for abundant beneficial insects in paddy field at Sungai Burong, specifically for insects of sub-orders Anisoptera and Zygoptera, and insects from families Gerridae and Coccinellidae. The plant height, insect pest, temperature, rainfall and humidity were found to be good predictors on the abundance of beneficial insects; nevertheless, the R2 value of multiple regression models was relatively low due to significant relationship between water parameters and insects. Consideration factors of water parameters, climatic factors and plant traits were strongly correlated with those parameters and abundance of insects in the paddy field at Sungai Burong, Tanjung Karang, Selangor.

The Mulberry Silkworm--A New Source of Bioactive Proteins

Beyond the commonly known proteins built silk fibres--fibroin and sericin, there are almost 300 bioactive proteins in the silkworm haemolymph. The aim of this work was to present bioactive compounds obtained from the silk fibre and isolated from the body of this insect, which may be used in medical and pharmacological applications. The most important are bioactive proteins. However, the juvenile stages of mulberry silkworm possess other very valuable active substances.

HORMONAL CONTROL OF THE VITELLOGENESIS IN THE JAPANESE OAK SILKWORM, ANTHERAEA YAMAMAI (LEPIDOPTERA : SATURNIIDAE)

Effects of ecdysteroid and juvenile hormone (JH) on vitellogenesis of the Japanese oak silkworm, Antheraea yamamai are reported in this article. After topical treatment with 20 hydroxyecdysone alone or JH analog (i.e. methoprene) alone and combined treatment with these two chemicals, vitellogenin (Vg) titers in the fat body and haemolymph at the pupal stage were mostly higher than those of the control, indicating that both ecdysteroid and JH exerted a promoting effect on the synthesis of Vg. In contrast, the Vg uptake was markedly inhibited by JH while stimulating effect of the ecdysteroid could be shown that vitellin (Vt) titer in the ovary was lower after methoprene treatments, but higher after 20 hydroxyecdyson treatments. Meanwhile, effects of these two hormones on Vg synthesis in the fat body were also tested with the incubation in vitro with Grace medium containing 3H leucine and the hormones. The results demonstrated that Vg synthesis was stimulated after treating with methoprene alone or 20 hydroxyecdysone alone and combined treating with these two chemicals, and particularly ecdysteroid had more marked positive effect. To comprehensively concluded our results, it could be regarded that ecdysteroid play the main role in the regulation of vitellogenesis for the Japanese oak silkworm.

Effect of elevated CO2 and O3 on phytohormone-mediated plant resistance to vector insects and insect-borne plant viruses

Climatic variations are becoming important limiting factors for agriculture productivity,as they not only directly affect the plant net primary productivity but can also modulate the outbreak of plant diseases and pests.Elevated CO_2 and O_3 are two important climatic factors that have been widely studied before.Elevated CO_2 or O_3 alters the host plant physiology and affects the vector insects and plant viruses via bottom-up effects of the host plants.Many studies have shown that elevated CO_2 or O_3 decreases the plant nitrogen content,which modulates the characteristics of vector insects.Recent evidence also reveals that hormone-dependent signaling pathways play a critical role in regulating the response of insects and plant viruses to elevated CO_2 or O_3.In the current review,we describe how elevated CO_2 or O_3 affects the vector insects and plant viruses by altering the SA and JA signaling pathways.We also discuss how changes in the feeding behavior of vector insects or the occurrence of plant viruses affects the interactions between vector insects and plant viruses under elevated CO_2 or O_3.We suggest that new insights into the upstream network that regulates hormone signaling and top-down effects of natural enemies would provide a comprehensive understanding of the complex interactions taking place under elevated CO_2 or O_3.

Effect of Qilongtoutong granule on calcitonin gene-related peptide,beta-endorphin, serotonin, dopamine, and noradrenalin in migraine model rats and mice

OBJECTIVE： To study the effect of Qilongtoutong granule （QLTT） on plasma calcitonin gene-related peptide （CGRP）, beta-endorphin （I[3-EP）, 5-HT, dopa- mine （DA）, noradrenalin （NE）, and blood viscosity in migraine model rats and mice. METHODS： Both the acute blood stasis model group and nitroglycerin-induced migraine model group included 60 Sprague-Dawley rats. The reser- pine-reduced model group had 60 Kunming mice. Rats from each test were grouped into normal con- trol group, model group, Zhengtian pill （ZTP） group, and high, moderate, or low-dose QLTT groups. In the acute blood stasis model test, after gavage for 7 days, rats were given 0.8 mL/kg adren- aline hydrochloride subcutaneously twice, and kept in ice water for 5 min. After fasting for 12 h, rats were anesthetized and blood samples were collected for detection of blood viscosity. In the nitro- glycerin-induced migraine group, after gavage for 7 days, rats were intraperitoneally injected nitro- glycerin （10 mg/kg）, and 4 h later, blood samples were collected from postcava for measuring the plasma CGRP and 13-EP levels. In the reserpine-re- duced model test, except the normal control group, mice were administered reserpine （0.25 mg/ kg, i.h.） for 9 days. Mice received intragastric admin- istration from the third day to the ninth day. One hour after the last gavage, blood and brain tissue samples were obtained. Then, blood clotting time and the contents of neurotransmitters were deter- mined. RESULTS： QLTT- （3.6, 1.8, and 0.9 g/kg） and ZTP-treated rats had lower blood viscosity than that in model rats under different shear rates （P〈 0.01）. QLTT- （3.6, 1.8 g/kg） and ZTP-treated rats had significantly lower plasma CGRP levels and higher plasma 13-EP levels than those in model rats （P〈 0.01）. QLTT treatment at dose of 0.9 g/kg had lower plasma CGRP levels as well （P〈0.05）. QLTT- （5.2, 2.6 g/kg） and ZTP-treated mice had longer blood clotting time than that in model mice （P〈0.01）. QLTT- （2.6 g/kg） and ZTP-treated mice had higher plasma serotonin （5-HT） levels than those in model mice （P〈0.05）. CONCLUSION： QLTT-treated animals had lower plasma CGRP level, higher plasma 13-EP, 5-HT, high- er brain tissue 5-HT, NE, DA levels, and lower blood viscosity than those in the migraine model animals.

Binding of Cry δ-endotoxins to brush border membrane vesicles of Helicoverpa armigera and Helicoverpa punctigera （Lepidoptera： Noctuidae）

The relatively low susceptibility ofHelicoverpa armigera to CrylAc, its history of resistance to chemical insecticides and the seasonal decline in expression of CrylAc in transgenic cotton necessitated the development of cotton expressing two insecticidal proteins to provide sustainable control of this multinational pest. To manage the resistance issue, it was essential that the second insecticidal protein have a significantly different mode of action to CrylAc. A common feature of resistance to CrylA proteins in several species as well as H. armigera has been a change in the binding site. A study of binding sites for some Cry proteins in the brush border membrane vesicles （BBMV） ofH. armigera and Helicoverpa punctigera was undertaken. The binding affinity for CrylAc was higher than for CrylAb, matching their relative toxicities, and CrylAc and CrylAb were found to share at least one binding site in both I-1. armigera and I-1. punctigera. However Cry2Aa did not compete with CrylAc for binding and so could be used in transgenic cotton in combination with CrylAc to control H. armigera and manage resistance. Variation in the susceptibilities of three different H. armigera strains to CrylAc correlated with the parameter Bmax/Kcom.

Mechanism of action of two insect toxins huwentoxin-Ⅲ and hainantoxin-Ⅵ on voltage-gated sodium channels

Selenocosmia huwena and Selenocosmia hainana are two tarantula species found in southern China.Their venoms contain abundant peptide toxins.Two new neurotoxic peptides,huwentoxin-Ⅲ(HWTX-Ⅲ) and hainantoxin-VI(HNTX-VI),were obtained from the venom using ion-exchange chromatography and reverse-phase high performance liquid chromatography(RP-HPLC).The mechanism of action of HWTX-Ⅲ and HNTX-VI on insect neuronal voltage-gated sodium channels(VGSCs) was studied via whole-cell patch clamp techniques.In a fashion similar to δ-atracotoxins,HNTX-VI can induce a slowdown of current inactivation of the VGSC and reduction in the peak of Na+ current in cockroach dorsal unpaired median(DUM) neurons.Meanwhile,10 μmol/L HNTX-IV caused a positive shift of steady-state inactivation of sodium channel.HWTX-ⅡI inhibited VGSCs on DUM neurons(concentration of toxin at half-maximal inhibition(IC50)≈1.106 μmol/L) in a way much similar to tetrodotoxin(TTX).HWTX-Ⅲ had no effect on the kinetics of activation and inactivation.The shift in the steady-state inactivation curve was distinct from other depressant spider toxins.The diverse effect and the mechanism of action of the two insect toxins illustrate the diverse biological activities of spider toxins and provide a fresh theoretical foundation to design and develop novel insecticides.

Partial purification of Cytochrome P450 from Helicoverpa armigera （Hübner）

The cytochrome P450 （Cyt-P450） proteins from the fat body and midgut of the cotton bollworm, Helicoverpa armigera, were respectively partially purified by a set of purification procedures including differential centrifugation, solubilization of CHAPS, protein precipitation by PEG precipitation and DE-32 column chromatography. The CytP450 was detected by methods of CO difference spectrum and SDS-PAGE. Fraction of detergent solubilized microsomes from the fat body of H. armigera was purified more than 17-fold. Three protein bands were detected by SDS-PAGE with molecular masses of 70 600, 63 300 and 571 200Da. It is possible that the proteins with molecular mass of 63 300 and 571 200Da were the isozymes of Cyt-P450.