An optimal investment strategy is extremely worth discussing and studying in our actual investment activities, not only for individual investors and companies but also for governments and all other kinds of investors....An optimal investment strategy is extremely worth discussing and studying in our actual investment activities, not only for individual investors and companies but also for governments and all other kinds of investors. And there is no doubt that we cannot find the only way and meet the needs of every investor. But if we can get an authoritative ranking according to the needs of investors, we can find the different best investment portfolio for different investors who have different view of risk theoretically and practically. From a typical case. all about these problems is what I will discuss in this article.展开更多
To reduce thrust ripple and cost and improve the average thrust of permanent magnet linear motors,a modular dual-field modulation permanent magnet linear motor was studied,and the parameters were optimized.First,sensi...To reduce thrust ripple and cost and improve the average thrust of permanent magnet linear motors,a modular dual-field modulation permanent magnet linear motor was studied,and the parameters were optimized.First,sensitive parameters were selected using the Taguchi method,and then the optimal variables were sampled using the optimal Latin hypercube experimental design method and an ensemble of surrogates model of optimization objectives,and its accuracy was verified.Next,a multi-objective particle swarm optimization algorithm was used to optimize the purpose of“maximum average thrust and minimum thrust ripple”,and the Pareto front of average thrust and thrust ripple was obtained.Finite element analysis showed that the optimized modular dual flux-modulation permanent magnet linear motor(MDFMPMLM)had a 29.5%reduction in thrust ripple and a 5%increase in average thrust compared to the original motor.This study provided an effective method for improving the performance of permanent magnet linear motors.展开更多
Four different types of three-body model composed of rock and coal with different strength and stiffness were established in order to study the failure characteristics of compound model such as roof-coal-floor. Throug...Four different types of three-body model composed of rock and coal with different strength and stiffness were established in order to study the failure characteristics of compound model such as roof-coal-floor. Through stress analysis of the element with variable strength and stiffness extracted from the strong-weak interface, the tri-axial compressive strength of the weak body and strong body near the interface as well as the areas away from the contact surface was found. Then, on the basis of three-dimensional fast Lagrangian method of continua and strain softening constitutive model composed of Coulomb-Mohr shear failure with tensile cut-off, stress and strain relationship of the four three-body combined models were analyzed under different confining pressures by numerical simulation. Finally, the different features of local shear zones and plastic failure areas of the four different models and their development trend with increasing confining pressure were discussed. The results show that additional stresses are derived due to the lateral deformation constraints near the strong-weak interface area, which results in the strength increasing in weak body and strength decreasing in strong body. The weakly consolidated soft rock and coal cementation exhibit significant strain softening behavior and bear compound tension-shear failure under uni-axial compression. With the increase of confining pressure, the tensile failure disappears from the model, and the failure type of composed model changes to local shear failure with different number of shearing bands and plastic failure zones. This work shows important guiding significance for the mechanism study of seismic, rock burst, and coal bump.展开更多
The current portfolio model for property-liability insurance company is only single period that can not meet the practical demands of portfolio management, and the purpose of this paper is to develop a multiperiod mod...The current portfolio model for property-liability insurance company is only single period that can not meet the practical demands of portfolio management, and the purpose of this paper is to develop a multiperiod model for its portfolio problem. The model is a multistage stochastic programming which considers transaction costs, cash flow between time periods, and the matching of asset and liability; it does not depend on the assumption for normality of return distribution. Additionally, an investment constraint is added. The numerical example manifests that the multiperiod model can more effectively assist the property-liability insurer to determine the optimal composition of insurance and investment portfolio and outperforms the single period one.展开更多
Influence of the pouring temperature ranging from 680 to 780 ℃ on the solidification behavior, the microstructure and mechanical properties of the sand-cast Mg-10Gd-3Y-0.4Zr alloy was investigated. It was found that ...Influence of the pouring temperature ranging from 680 to 780 ℃ on the solidification behavior, the microstructure and mechanical properties of the sand-cast Mg-10Gd-3Y-0.4Zr alloy was investigated. It was found that the nucleation undercooling of the a-Mg phase increased from 2.3 to 6.3 ℃. The average a-Mg grain size increased from 44 to 71 μm, but then decreased to 46 μm. The morphology of the eutectic compound transformed from a continuous network into a discontinuous state and then subsequently into an island-like block. The volume fraction of β-Mg_24RE_5 phase increased and its morphology transformed from particle into rod-like. The increase in pouring temperature increased the solute concentration. YS increased from 138 to 151 MPa, and UTS increased from 186 to 197 MPa. The alloy poured at 750 ℃ had optimal combining strength and ductility. The fracture surface mode transformed from quasi-cleavage crack into transgranular fracture, all plus the dimple-like fracture, with the micro-porosity and the re-oxidation inclusion as major defects. The average a-Mg grain size played a main role in the YS of sand-cast Mg-10Gd-3Y-0.4Zr alloy, besides other factors, i.e. micro-porosity, morphology of eutectic compounds, re-oxidation inclusion and solute concentration.展开更多
Building a model for a object through model composition is a interesting topics, this paper research the interface composit ion of models in the Zeigler’s modeling methodology DEVS.
For the feature of complex weapon manufacturing on internet,a coupling model is proposed.By using the model,the correlation between manufacturing cells in an extended manufacturing organization can be evaluated quanti...For the feature of complex weapon manufacturing on internet,a coupling model is proposed.By using the model,the correlation between manufacturing cells in an extended manufacturing organization can be evaluated quantitatively,so an appropriate control plan is determined.A strategy to improve and reduce the coupling relationship of the organization is studied.A correlation matrix of extended tasks is built to analyze the relationship between sub-tasks and manufacturing resources.An optimization method for manufacturing resource configuration is presented based on the coupling model.Finally,a software system for analyzing coupling model about manufacturing organization on internet is developed,and the result shows that the coupling model is effective.展开更多
To improve the accuracy and speed in cycle-accurate power estimation, this paper uses multiple dimensional coefficients to build a Bayesian inference dynamic power model. By analyzing the power distribution and intern...To improve the accuracy and speed in cycle-accurate power estimation, this paper uses multiple dimensional coefficients to build a Bayesian inference dynamic power model. By analyzing the power distribution and internal node state, we find the deficiency of only using port information. Then, we define the gate level number computing method and the concept of slice, and propose using slice analysis to distill switching density as coefficients in a special circuit stage and participate in Bayesian inference with port information. Experiments show that this method can reduce the power-per-cycle estimation error by 21.9% and the root mean square error by 25.0% compared with the original model, and maintain a 700 + speedup compared with the existing gate-level power analysis technique.展开更多
In the present paper, RAPD was used to study the genetic polymorphism of fisheswith different genome combinations. Our results indicated that four of the 26 random primersproduced distinct and reproducible electrophor...In the present paper, RAPD was used to study the genetic polymorphism of fisheswith different genome combinations. Our results indicated that four of the 26 random primersproduced distinct and reproducible electrophoretic patterns which were genome-specific andcould distinguish different biotypes. This enabled us to derive a diagnostic profile, from whichwe constructed a molecular marker key for different biotypes. By the analysis of the data ofRAPD patterns, the genetic relationship was constructed with UPGMA (unweighted pair-groupmethod with arithmetical averages). Our experiments also concluded that RAPD was moresuccessful in variety identification than protein polymorphism analysis and serohematology for itstechnological simplicity and sensitivity.展开更多
A kind of combining forecasting model based on the generalized weighted functional mean is proposed. Two kinds of parameter estimation methods with its weighting coefficients using the algorithm of quadratic programmi...A kind of combining forecasting model based on the generalized weighted functional mean is proposed. Two kinds of parameter estimation methods with its weighting coefficients using the algorithm of quadratic programming are given. The efficiencies of this combining forecasting model and the comparison of the two kinds of parameter estimation methods are demonstrated with an example. A conclusion is obtained, which is useful for the correct application of the above methods.展开更多
Most edge-detection methods rely on calculating gradient derivatives of the potential field, a process that is easily affected by noise and is therefore of low stability. We propose a new edge-detection method named c...Most edge-detection methods rely on calculating gradient derivatives of the potential field, a process that is easily affected by noise and is therefore of low stability. We propose a new edge-detection method named correlation coefficient of multidirectional standard deviations(CCMS) that is solely based on statistics. First, we prove the reliability of the proposed method using a single model and then a combination of models. The proposed method is evaluated by comparing the results with those obtained by other edge-detection methods. The CCMS method offers outstanding recognition, retains the sharpness of details, and has low sensitivity to noise. We also applied the CCMS method to Bouguer anomaly data of a potash deposit in Laos. The applicability of the CCMS method is shown by comparing the inferred tectonic framework to that inferred from remote sensing(RS) data.展开更多
In order to formally reason and verify web services composition described by web services choreography specification WS-CDL,a typed formal model named typed Abstract WS-CDL(web services choreography description langu...In order to formally reason and verify web services composition described by web services choreography specification WS-CDL,a typed formal model named typed Abstract WS-CDL(web services choreography description language)for WS-CDL specifications is proposed.In typed Abstract WS-CDL,the syntax of type and session,typing rules and operational semantics are formalized;the collaborations of web services are formally described by sessions;the operational semantics of a session can help to formally reason the execution of the choreography;the typing rules can help to formally check the data type consistency of exchanged information between web services and capture run-time errors due to type mismatches.Particularly,the concepts of type assumption set extension and type assumption set compatibility are proposed,and the merging algorithm of type assumption sets is defined so as to eliminate type assumption conflict.Based on the formal model,typed mapping rules for mapping web services choreography to orchestration is also defined.With the typed mapping rules,orchestration stubs and their type assumption sets can be generated from a given choreography; thus, web services composition can be verified at choreography and orchestration levels,respectively.The model is proved to have properties of type safety,and how the model can help to reason and verify web services composition is illustrated through a case study.展开更多
In order to improve the effectiveness of the artificial intelligence (AI) planning based web service composition problem, a business domain oriented AI planning algorithm for web service composition is introduced. T...In order to improve the effectiveness of the artificial intelligence (AI) planning based web service composition problem, a business domain oriented AI planning algorithm for web service composition is introduced. The algorithm uses the proposed business relation model to minimize the searching space and uses the proposed goal distance as a heuristic rule. Compared with other algorithms tackling web service composition such as AI planning, the proposed algorithm focuses not only on how to convert the web service composition problem to the AI planning problem, but also exerts its effort on how to apply the business feature of web service composition into planning in order to improve the effectiveness. The experiment shows the better performance of the proposed algorithm.展开更多
AIM To explore a rapid and easy sequencing method for hepatitis C virus (HCV) genome, and establish a new sequencing method in China. METHODS Polymerase Chain Reaction (PCR) was combined with DNA sequencing techn...AIM To explore a rapid and easy sequencing method for hepatitis C virus (HCV) genome, and establish a new sequencing method in China. METHODS Polymerase Chain Reaction (PCR) was combined with DNA sequencing technique. PCR products were purified by agarose gel electrophoresis, polyacrylamide gel electrophoresis (PAGE), Polyethylene glycol (PEG) respectively. Then in the presence of a 5′ labeling PCR primer, purified PCR products were directly sequenced. By this method, HCV NS5b cDNA from two HCV infected individuals (HC 42 and HC 49) were sequenced.展开更多
Traffic flow prediction,as the basis of signal coordination and travel time prediction,has become a research point in the field of transportation.For traffic flow prediction,researchers have proposed a variety of meth...Traffic flow prediction,as the basis of signal coordination and travel time prediction,has become a research point in the field of transportation.For traffic flow prediction,researchers have proposed a variety of methods,but most of these methods only use the time domain information of traffic flow data to predict the traffic flow,ignoring the impact of spatial correlation on the prediction of target road segment flow,which leads to poor prediction accuracy.In this paper,a traffic flow prediction model called as long short time memory and random forest(LSTM-RF)was proposed based on the combination model.In the process of traffic flow prediction,the long short time memory(LSTM)model was used to extract the time sequence features of the predicted target road segment.Then,the predicted value of LSTM and the collected information of adjacent upstream and downstream sections were simultaneously used as the input features of the random forest model to analyze the spatial-temporal correlation of traffic flow,so as to obtain the final prediction results.The traffic flow data of 132 urban road sections collected by the license plate recognition system in Guiyang City were tested and verified.The results show that the method is better than the single model in prediction accuracy,and the prediction error is obviously reduced compared with the single model.展开更多
Cu-0.36 wt.%Be-0.46 wt.%Co alloy plate with 300 mm in width and 10 mm in thickness prepared by heating-cooling combined mold(HCCM) horizontal continuous casting was cold rolled. Microstructure evolution and mechanical...Cu-0.36 wt.%Be-0.46 wt.%Co alloy plate with 300 mm in width and 10 mm in thickness prepared by heating-cooling combined mold(HCCM) horizontal continuous casting was cold rolled. Microstructure evolution and mechanical properties of the alloy as well as its deformation mechanism were investigated. The results showed that the as-cast alloy plate had columnar grains along the length direction, good surface quality and elongation of 35%, which was directly large-reduction cold rolled without surface treatment, and the accumulative cold rolling reduction reached 98%. When the reduction was small(20%), numerous dislocations and dislocation cells formed, and the deformation mechanism was dislocation slip. When the reduction was 40%, deformation twins appeared, and interactions between twins and dislocation cells induced strip-like dislocation cells. When the reduction exceeded 60%, shear bands formed and apparent crystal rotation in the micro-region happened. Further increasing the reduction, the amount of the shear bands rose and they interacted with each other, which refined the grains apparently. The tensile strength and hardness increased from 353 MPa and HV 119 of the as-cast alloy to 625 MPa and HV 208 with 95% reduction, respectively, and the elongation reduced from 35% to 7.6%. A process of HCCM horizontal continuous casting-cold rolling can work as a novel compact method to fabricate Cu-Be alloy sheet.展开更多
As an important factor in evaluating service,QoS(Quality of Service) has drawn more and more concerns with the rapid increasing of Web services. However,due to the great volatility of services in Mobile Internet envir...As an important factor in evaluating service,QoS(Quality of Service) has drawn more and more concerns with the rapid increasing of Web services. However,due to the great volatility of services in Mobile Internet environments,such as internet of vehicles,Web services often do not work as announced and thus cause unacceptable problems. QoS prediction can avoid failure before it takes place,which is considered a more effective way to assure quality. However,Current QoS prediction approaches neither consider the highly dynamic of Web services,nor maintain good prediction performance all the time. Consequently we propose a novel Bayesian combinational model to predict QoS by continuously adjusting credit values of the basic models so as to keep good prediction accuracy. QoS attributes such as response time,throughput and reliability are used to validate the proposed model. Experimental results show that the model can provide stable prediction results in Mobile Internet environments.展开更多
Modeling of solute transport is a key issue in the area of soil physics and hydrogeology. The most common approach (the convection-dispersion equation) considers an average convection flow rate and Fickian-like disper...Modeling of solute transport is a key issue in the area of soil physics and hydrogeology. The most common approach (the convection-dispersion equation) considers an average convection flow rate and Fickian-like dispersion. Here,we propose a solute transport model in porous media of continuously expanding scale, according to the combinatorics principle. The model supposed actual porous media as a combinative body of many basic segments. First, we studied the solute transport process in each basic segment body, and then deduced the distribution of pore velocity in each basic segment body by difference approximation, finally assembled the solute transport process of each basic segment body into one of the combinative body. The simulation result coincided with the solute transport process observed in test. The model provides useful insight into the solute transport process of the non-Fickian dispersion in continuously expanding scale.展开更多
文摘An optimal investment strategy is extremely worth discussing and studying in our actual investment activities, not only for individual investors and companies but also for governments and all other kinds of investors. And there is no doubt that we cannot find the only way and meet the needs of every investor. But if we can get an authoritative ranking according to the needs of investors, we can find the different best investment portfolio for different investors who have different view of risk theoretically and practically. From a typical case. all about these problems is what I will discuss in this article.
文摘To reduce thrust ripple and cost and improve the average thrust of permanent magnet linear motors,a modular dual-field modulation permanent magnet linear motor was studied,and the parameters were optimized.First,sensitive parameters were selected using the Taguchi method,and then the optimal variables were sampled using the optimal Latin hypercube experimental design method and an ensemble of surrogates model of optimization objectives,and its accuracy was verified.Next,a multi-objective particle swarm optimization algorithm was used to optimize the purpose of“maximum average thrust and minimum thrust ripple”,and the Pareto front of average thrust and thrust ripple was obtained.Finite element analysis showed that the optimized modular dual flux-modulation permanent magnet linear motor(MDFMPMLM)had a 29.5%reduction in thrust ripple and a 5%increase in average thrust compared to the original motor.This study provided an effective method for improving the performance of permanent magnet linear motors.
基金Project(51174128)supported by the National Natural Science Foundation of ChinaProject(20123718110007)supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China
文摘Four different types of three-body model composed of rock and coal with different strength and stiffness were established in order to study the failure characteristics of compound model such as roof-coal-floor. Through stress analysis of the element with variable strength and stiffness extracted from the strong-weak interface, the tri-axial compressive strength of the weak body and strong body near the interface as well as the areas away from the contact surface was found. Then, on the basis of three-dimensional fast Lagrangian method of continua and strain softening constitutive model composed of Coulomb-Mohr shear failure with tensile cut-off, stress and strain relationship of the four three-body combined models were analyzed under different confining pressures by numerical simulation. Finally, the different features of local shear zones and plastic failure areas of the four different models and their development trend with increasing confining pressure were discussed. The results show that additional stresses are derived due to the lateral deformation constraints near the strong-weak interface area, which results in the strength increasing in weak body and strength decreasing in strong body. The weakly consolidated soft rock and coal cementation exhibit significant strain softening behavior and bear compound tension-shear failure under uni-axial compression. With the increase of confining pressure, the tensile failure disappears from the model, and the failure type of composed model changes to local shear failure with different number of shearing bands and plastic failure zones. This work shows important guiding significance for the mechanism study of seismic, rock burst, and coal bump.
文摘The current portfolio model for property-liability insurance company is only single period that can not meet the practical demands of portfolio management, and the purpose of this paper is to develop a multiperiod model for its portfolio problem. The model is a multistage stochastic programming which considers transaction costs, cash flow between time periods, and the matching of asset and liability; it does not depend on the assumption for normality of return distribution. Additionally, an investment constraint is added. The numerical example manifests that the multiperiod model can more effectively assist the property-liability insurer to determine the optimal composition of insurance and investment portfolio and outperforms the single period one.
基金Project(51275295)supported by the National Natural Science Foundation of ChinaProject(USCAST2012-15)supported by the Funded Project of SAST-SJTU Joint Research Centre of Advanced Aerospace Technology,ChinaProject(20130073110052)supported by the Research Fund for the Doctoral Program of Higher Education of China
文摘Influence of the pouring temperature ranging from 680 to 780 ℃ on the solidification behavior, the microstructure and mechanical properties of the sand-cast Mg-10Gd-3Y-0.4Zr alloy was investigated. It was found that the nucleation undercooling of the a-Mg phase increased from 2.3 to 6.3 ℃. The average a-Mg grain size increased from 44 to 71 μm, but then decreased to 46 μm. The morphology of the eutectic compound transformed from a continuous network into a discontinuous state and then subsequently into an island-like block. The volume fraction of β-Mg_24RE_5 phase increased and its morphology transformed from particle into rod-like. The increase in pouring temperature increased the solute concentration. YS increased from 138 to 151 MPa, and UTS increased from 186 to 197 MPa. The alloy poured at 750 ℃ had optimal combining strength and ductility. The fracture surface mode transformed from quasi-cleavage crack into transgranular fracture, all plus the dimple-like fracture, with the micro-porosity and the re-oxidation inclusion as major defects. The average a-Mg grain size played a main role in the YS of sand-cast Mg-10Gd-3Y-0.4Zr alloy, besides other factors, i.e. micro-porosity, morphology of eutectic compounds, re-oxidation inclusion and solute concentration.
文摘Building a model for a object through model composition is a interesting topics, this paper research the interface composit ion of models in the Zeigler’s modeling methodology DEVS.
基金Supported by the National Defense Industrial Technology Development Program of China~~
文摘For the feature of complex weapon manufacturing on internet,a coupling model is proposed.By using the model,the correlation between manufacturing cells in an extended manufacturing organization can be evaluated quantitatively,so an appropriate control plan is determined.A strategy to improve and reduce the coupling relationship of the organization is studied.A correlation matrix of extended tasks is built to analyze the relationship between sub-tasks and manufacturing resources.An optimization method for manufacturing resource configuration is presented based on the coupling model.Finally,a software system for analyzing coupling model about manufacturing organization on internet is developed,and the result shows that the coupling model is effective.
文摘To improve the accuracy and speed in cycle-accurate power estimation, this paper uses multiple dimensional coefficients to build a Bayesian inference dynamic power model. By analyzing the power distribution and internal node state, we find the deficiency of only using port information. Then, we define the gate level number computing method and the concept of slice, and propose using slice analysis to distill switching density as coefficients in a special circuit stage and participate in Bayesian inference with port information. Experiments show that this method can reduce the power-per-cycle estimation error by 21.9% and the root mean square error by 25.0% compared with the original model, and maintain a 700 + speedup compared with the existing gate-level power analysis technique.
文摘In the present paper, RAPD was used to study the genetic polymorphism of fisheswith different genome combinations. Our results indicated that four of the 26 random primersproduced distinct and reproducible electrophoretic patterns which were genome-specific andcould distinguish different biotypes. This enabled us to derive a diagnostic profile, from whichwe constructed a molecular marker key for different biotypes. By the analysis of the data ofRAPD patterns, the genetic relationship was constructed with UPGMA (unweighted pair-groupmethod with arithmetical averages). Our experiments also concluded that RAPD was moresuccessful in variety identification than protein polymorphism analysis and serohematology for itstechnological simplicity and sensitivity.
文摘A kind of combining forecasting model based on the generalized weighted functional mean is proposed. Two kinds of parameter estimation methods with its weighting coefficients using the algorithm of quadratic programming are given. The efficiencies of this combining forecasting model and the comparison of the two kinds of parameter estimation methods are demonstrated with an example. A conclusion is obtained, which is useful for the correct application of the above methods.
基金supported by the National Hi-Tech Research and Development Program of China(863 Program)(No.2006AA06Z107)the National Natural Science Foundation of China(No.40930314)
文摘Most edge-detection methods rely on calculating gradient derivatives of the potential field, a process that is easily affected by noise and is therefore of low stability. We propose a new edge-detection method named correlation coefficient of multidirectional standard deviations(CCMS) that is solely based on statistics. First, we prove the reliability of the proposed method using a single model and then a combination of models. The proposed method is evaluated by comparing the results with those obtained by other edge-detection methods. The CCMS method offers outstanding recognition, retains the sharpness of details, and has low sensitivity to noise. We also applied the CCMS method to Bouguer anomaly data of a potash deposit in Laos. The applicability of the CCMS method is shown by comparing the inferred tectonic framework to that inferred from remote sensing(RS) data.
基金The National Natural Science Foundation of China(No.60403027,60773191,70771043)the National High Technology Research and Development Program of China(863 Program)(No.2007AA01Z403)
文摘In order to formally reason and verify web services composition described by web services choreography specification WS-CDL,a typed formal model named typed Abstract WS-CDL(web services choreography description language)for WS-CDL specifications is proposed.In typed Abstract WS-CDL,the syntax of type and session,typing rules and operational semantics are formalized;the collaborations of web services are formally described by sessions;the operational semantics of a session can help to formally reason the execution of the choreography;the typing rules can help to formally check the data type consistency of exchanged information between web services and capture run-time errors due to type mismatches.Particularly,the concepts of type assumption set extension and type assumption set compatibility are proposed,and the merging algorithm of type assumption sets is defined so as to eliminate type assumption conflict.Based on the formal model,typed mapping rules for mapping web services choreography to orchestration is also defined.With the typed mapping rules,orchestration stubs and their type assumption sets can be generated from a given choreography; thus, web services composition can be verified at choreography and orchestration levels,respectively.The model is proved to have properties of type safety,and how the model can help to reason and verify web services composition is illustrated through a case study.
基金The National Key Technologies R & D Program of China during the 10th Five-Year Plan Period(No2004BA721A05)
文摘In order to improve the effectiveness of the artificial intelligence (AI) planning based web service composition problem, a business domain oriented AI planning algorithm for web service composition is introduced. The algorithm uses the proposed business relation model to minimize the searching space and uses the proposed goal distance as a heuristic rule. Compared with other algorithms tackling web service composition such as AI planning, the proposed algorithm focuses not only on how to convert the web service composition problem to the AI planning problem, but also exerts its effort on how to apply the business feature of web service composition into planning in order to improve the effectiveness. The experiment shows the better performance of the proposed algorithm.
文摘AIM To explore a rapid and easy sequencing method for hepatitis C virus (HCV) genome, and establish a new sequencing method in China. METHODS Polymerase Chain Reaction (PCR) was combined with DNA sequencing technique. PCR products were purified by agarose gel electrophoresis, polyacrylamide gel electrophoresis (PAGE), Polyethylene glycol (PEG) respectively. Then in the presence of a 5′ labeling PCR primer, purified PCR products were directly sequenced. By this method, HCV NS5b cDNA from two HCV infected individuals (HC 42 and HC 49) were sequenced.
文摘Traffic flow prediction,as the basis of signal coordination and travel time prediction,has become a research point in the field of transportation.For traffic flow prediction,researchers have proposed a variety of methods,but most of these methods only use the time domain information of traffic flow data to predict the traffic flow,ignoring the impact of spatial correlation on the prediction of target road segment flow,which leads to poor prediction accuracy.In this paper,a traffic flow prediction model called as long short time memory and random forest(LSTM-RF)was proposed based on the combination model.In the process of traffic flow prediction,the long short time memory(LSTM)model was used to extract the time sequence features of the predicted target road segment.Then,the predicted value of LSTM and the collected information of adjacent upstream and downstream sections were simultaneously used as the input features of the random forest model to analyze the spatial-temporal correlation of traffic flow,so as to obtain the final prediction results.The traffic flow data of 132 urban road sections collected by the license plate recognition system in Guiyang City were tested and verified.The results show that the method is better than the single model in prediction accuracy,and the prediction error is obviously reduced compared with the single model.
基金Project(2016YFB0301404)supported by the National Key R&D Program of China。
文摘Cu-0.36 wt.%Be-0.46 wt.%Co alloy plate with 300 mm in width and 10 mm in thickness prepared by heating-cooling combined mold(HCCM) horizontal continuous casting was cold rolled. Microstructure evolution and mechanical properties of the alloy as well as its deformation mechanism were investigated. The results showed that the as-cast alloy plate had columnar grains along the length direction, good surface quality and elongation of 35%, which was directly large-reduction cold rolled without surface treatment, and the accumulative cold rolling reduction reached 98%. When the reduction was small(20%), numerous dislocations and dislocation cells formed, and the deformation mechanism was dislocation slip. When the reduction was 40%, deformation twins appeared, and interactions between twins and dislocation cells induced strip-like dislocation cells. When the reduction exceeded 60%, shear bands formed and apparent crystal rotation in the micro-region happened. Further increasing the reduction, the amount of the shear bands rose and they interacted with each other, which refined the grains apparently. The tensile strength and hardness increased from 353 MPa and HV 119 of the as-cast alloy to 625 MPa and HV 208 with 95% reduction, respectively, and the elongation reduced from 35% to 7.6%. A process of HCCM horizontal continuous casting-cold rolling can work as a novel compact method to fabricate Cu-Be alloy sheet.
基金supported by National Natural Science Foundation of China (61572171,61202097,61202136)Research Fund for the Doctoral Program of Higher Education of China (20120094120009)+2 种基金Fundamental Research Funds for the Central Universities of China (B15020191)the national college students innovation training program (No.201511460012)by Jiangsu Province,and key special funds of efficient utilization of water resources (No.2016YFC0402710)
文摘As an important factor in evaluating service,QoS(Quality of Service) has drawn more and more concerns with the rapid increasing of Web services. However,due to the great volatility of services in Mobile Internet environments,such as internet of vehicles,Web services often do not work as announced and thus cause unacceptable problems. QoS prediction can avoid failure before it takes place,which is considered a more effective way to assure quality. However,Current QoS prediction approaches neither consider the highly dynamic of Web services,nor maintain good prediction performance all the time. Consequently we propose a novel Bayesian combinational model to predict QoS by continuously adjusting credit values of the basic models so as to keep good prediction accuracy. QoS attributes such as response time,throughput and reliability are used to validate the proposed model. Experimental results show that the model can provide stable prediction results in Mobile Internet environments.
文摘Modeling of solute transport is a key issue in the area of soil physics and hydrogeology. The most common approach (the convection-dispersion equation) considers an average convection flow rate and Fickian-like dispersion. Here,we propose a solute transport model in porous media of continuously expanding scale, according to the combinatorics principle. The model supposed actual porous media as a combinative body of many basic segments. First, we studied the solute transport process in each basic segment body, and then deduced the distribution of pore velocity in each basic segment body by difference approximation, finally assembled the solute transport process of each basic segment body into one of the combinative body. The simulation result coincided with the solute transport process observed in test. The model provides useful insight into the solute transport process of the non-Fickian dispersion in continuously expanding scale.