[Objective]The study was to analyze the karyotype of Allium macrostemon Bunge in Laizhou City of Shandong Province.[ Method] The root tip of A. macrostemon was pretreated with 8-hydroxyquinoline solution, fixed, disso...[Objective]The study was to analyze the karyotype of Allium macrostemon Bunge in Laizhou City of Shandong Province.[ Method] The root tip of A. macrostemon was pretreated with 8-hydroxyquinoline solution, fixed, dissociated and stained for preparing the glass slide to reveal the chromosome number via the microscopic examination; the sparse cells with good chromosome morphology were photographed under microscope. [ Result ]Allium macrostemon Bunge in Laizhou City introduced in this study was tdploid; its somatic chromosome number was 24 and karyotype formula was K（2n） =3x=24m（2SAT） ＋ 1Bs, thus the karyotype belongs to 1A type. One of the chromosome No. 3 contained satellite, and chromosome deletion may be existed in one of the chromosome No. 5. In addition, B chromosome was observed in some cells.[ Conclusion] This introduction of triploid A. macrostemon found in China was the first time.展开更多
In practical application,mean shift tracking algorithm is easy to generate tracking drift when the target and the background have similar color distribution.Based on the mean shift algorithm,a kind of background weake...In practical application,mean shift tracking algorithm is easy to generate tracking drift when the target and the background have similar color distribution.Based on the mean shift algorithm,a kind of background weaken weight is proposed in the paper firstly.Combining with the object center weight based on the kernel function,the problem of interference of the similar color background can be solved.And then,a model updating strategy is presented to improve the tracking robustness on the influence of occlusion,illumination,deformation and so on.With the test on the sequence of Tiger,the proposed approach provides better performance than the original mean shift tracking algorithm.展开更多
In order to develop green good, reduce the use of chemical fertilizer and pesticide, realize ＂double reduction＂ and synergism, improve safety and quality of products, protect the ecological environment and promote t...In order to develop green good, reduce the use of chemical fertilizer and pesticide, realize ＂double reduction＂ and synergism, improve safety and quality of products, protect the ecological environment and promote the healthy and steady development of strawberry industry, the goals and key technology of fertilizer-pesticide ＂double reduction＂ and synergism for greenhouse strawberry are summed up in this paper targeting at the current situation of strawberry production and combined with the new technology achievements at home and abroad. The key technology includes six items： healthy seedling cultivating technique, soil improvement and continuous cropping obstacle treatment technique, agricultural ecological prevention tech- nique, physicochemical trap of pest control technique, biological control technique and low residual risk chemical control technique.展开更多
Though Yurman province contains some 562 known species of fish, no cell lines from any of these have been made available to date. To protect germplasm resources and provide an effective tool in solving problems at cel...Though Yurman province contains some 562 known species of fish, no cell lines from any of these have been made available to date. To protect germplasm resources and provide an effective tool in solving problems at cellular level of Anabarilius grahami, a fish endemic to Fuxian Lake, Yunnan, China, we established and characterized the major features of a continuous cell line （AGF II） from the caudal fin tissue of A. grahami. This AGF II cell line consists of fibroblast-like cells and has been subeultured more than 60 times over the course of a year. The cell line was maintained in DMEM/F12 supplemented with 10% FBS, with a cellular doubling time of 51.1 h. We continued with more experiments to optimize the culture and storage conditions, and found a variety of interesting results： cells could grow at temperature between 24 ~C and 28 ~C, with the optimal temperature of 28 ~C. Likewise, the growth rate ofA. grahami fin cells increased when the FBS proportion increased from 5% to 20%, with the optimal growth at the concentrations of 20% FBS, cells were able to grow in L-15 and DMEM/FI2 with optimal growth at L-15; DMSO is a better eryoprotectant than Glycerol, EG and MeOH for AGFII cells with optimal concentration of 5% DMSO. Chromosome analysis also showed that the distribution of chromosome number varies from 38 to 52, with a modal peak at 48 chromosomes, accounting for 39.8% of all cells. Using the same primer pairs specific to mtDNA, the AGF II cell sequences obtained by PCR were identical to those from muscle tissues ofA. grahami. Both chromosome analysis and PCR amplification confirmed the AGF II cells were from A. grahami, also indicating that that current long-term artificial propagation ofA. grahami has been successful. Finally, we noted that when cells were transfected with pEYFP-N1 and pECFP-N1 plasmid, bright fluorescent signals were observed, suggesting that this cell line may be suitable for use in transfection and future gene expression studies.展开更多
[Objective] This study was conducted to obtain tetraploid Pteroceltis tatar# nowii Maxim. with excellent ornamental traits. [Method] The stem apex growing points of Pteroceltis tatarinowii Maxim. were treated with dif...[Objective] This study was conducted to obtain tetraploid Pteroceltis tatar# nowii Maxim. with excellent ornamental traits. [Method] The stem apex growing points of Pteroceltis tatarinowii Maxim. were treated with different concentrations of colchicine solution for different hours to figure out a proper method and obtain poly- ploids. [Result] The most effective induction was obtained by treatment with 0.6%- 0.8% colchicine for 72 h with 34.2% mutation rate. Flow cytometry and chromosome observation of the stem apex growing point of P. tatarinowii Maxim. proved that the tetraploid plants were successfully obtained with chromosome number 2n=4x=36. [Conclusion] The result not only fills the blank of polyploid breeding of P. tatarinowii, but also provides an effective way to broaden the methods of cultivation of fast- growing, high-quality, disease-resilience, new varieties of Pteroceltis.展开更多
The somaclone, C39, derived by tissue culture from the obligate apomict Paspalum dilatatum cv Raki (2n=50), had 50 chromosomes and a karyotype apparently identical to Raki.SC2 seedlings of C39 showed a high degree of ...The somaclone, C39, derived by tissue culture from the obligate apomict Paspalum dilatatum cv Raki (2n=50), had 50 chromosomes and a karyotype apparently identical to Raki.SC2 seedlings of C39 showed a high degree of phenotypic variation which was of ten associated with increased chromosome numbers, but some of the variant seedlings were karyotypically indistinguishable from Raki or C39. Plants with increased chromosome numbers exhibited a high degree of intraplant chromosome variation (aneusomaty). In one of the SC2seedlings, the chromosome number of root tip cells varied from 58 to 82 and in several other seedlings the range was more than 10. The results suggested that the ability to form seed apomictically was much reduced in C39 and that this plant showed some capacity for sexual reproduction and the resulting seedlings, with a chromosome number of about 70, were genetically unstable. Of 11 SC2 seedlings examined cytolog ically, 6 did not produce any viable seed. Seedlings grown from seed of the remaining 5 plants showed that aneusomaty persisted in the SC3 generation. SC3 seedlings which were phenotypically similar to their maternal parent showed a similar range of chromosome numbers to that parent. Some of the SC3 seedlings exhibited an even wider range of chromosome numbers (e.g.56-136), and these plants were all dwarfs.展开更多
We performed a karyotype analysis on four species from Phylum Nemertea using regenerating somatic cells. Two palaeonemertean species, Cephalothrix hongkongiensis and Cephalothrix sp., had the same chromosome number （...We performed a karyotype analysis on four species from Phylum Nemertea using regenerating somatic cells. Two palaeonemertean species, Cephalothrix hongkongiensis and Cephalothrix sp., had the same chromosome number （2n=28）, but different karyotypes （16 m＋10 sm＋2 st, NF=54 and 22 m＋4 sm＋2 st, NF=54, respectively）. The karyotypes of the two heteronemerteans, Notospermus geniculatus and Ramphogordius sanguineus were 2n=38, 30 m＋6 sm＋2 sm/st （NF=76/74） and 2n=56, 48 m＋4 sm＋4 sin/st CNF=112/108）, respectively.展开更多
The present paper deals with the genus Willowsia and its Chinese species. A new species, Willowsia guangxiensis, sp. nov., is described from Guangxi of South China.
For dealing with high-salinity wastewater in the refinery, the high cost of driving heat source is the disadvantage of multi-effect distillation (MED) so it is of great importance to evaluate the performance of low-te...For dealing with high-salinity wastewater in the refinery, the high cost of driving heat source is the disadvantage of multi-effect distillation (MED) so it is of great importance to evaluate the performance of low-temperature heat source for conducting MED and select the optimal temperature for it. Both the MED and the low-temperature heat sources studied in this paper were from a typical refinery located in northwestern China. Besides, a new methodology to evaluate heat sources as the optimal candidate was proposed for MED based on the grey system theory. Five process units, which included 18 fluids of the refinery, were named as the evaluation projects. Three factors, which included safety effects, total costs and characteristics of low-temperature heat sources were determined as the evaluation indexes, the values of which were established through the analyses. The results obtained through the grey correlation analyses have revealed that the grey correlation degrees of these units were 0.661(AVDU), 0.732 (#1 FCCU), 0.618 (#2 FCCU), 0.535 (#1 DCU), and 0.572 (#2 DCU), respectively. Thus, the optimal heat source was provided from #1 FCCU. Through further analyses of the fluids from #1 FCCU, the grey correlation degrees of the fluids were 0.597 (oil and gas at top of tower), 0.714 (recycle oil and gas), and 0.512 (diesel), respectively. Thus, the optimal heat source was the oil and gas recycle stream.展开更多
This paper reports the occurrence of a marine brachyuran crab species Eucrate alcocki Serene, in Serene et al., 1973, of the family Euryplacidae Stimpson, 1871, for first time from India, based on a male specimen from...This paper reports the occurrence of a marine brachyuran crab species Eucrate alcocki Serene, in Serene et al., 1973, of the family Euryplacidae Stimpson, 1871, for first time from India, based on a male specimen from Parangipettai fish landing centre in Bay of Bengal, Southeast Coast of India. Although morphologically corresponding with what is currently defined as E. alcocki, the color pattern of the carapace of the present specimen is rather different from that of the Chinese material-only the anterior fifth of the carapace is marked with scattered red spots, the rest of the surface is yellowish, with four unusually shaped red blotches which almost look like Sanskrit characters.展开更多
High quality polytene chromosome maps （n=3） of a Himalayan Simuliid Simulium praelargum Datta, 1973 are presented and represent the first cytological description of a taxon found in the feuerborni group, subgenus Ne...High quality polytene chromosome maps （n=3） of a Himalayan Simuliid Simulium praelargum Datta, 1973 are presented and represent the first cytological description of a taxon found in the feuerborni group, subgenus Nevermannia. Polytene chromosomes one （I） and two （II） are metacentric, chromosome three （III） is submetacentric with the length of each chromosome occupying 37.25 %, 31.36 % and 31.34 % of the total complement length, respectively. Typical simuliid diagnostic intergeneric chromosomal markers are found within the polytene complement of this species. The nucleolar organizer （N.O.） is found at the base of the short arm of chromosome one （IS）, the Ring of Balbiani （R.B.）, double bubble （D.B.） and triad occur in the short arm of chromosome two （IIS）, the Parabalbiani Ring （EB.） and grey band （gb） occur in the long arm of chromosome two （IIL） and the Blister （BL） and Capsule （Ca） occur in the short arm of chromosome three （IIIS）.Terminal bands at the end of IIIS are heterochromatinized and present atypically with respect to other simuliid fauna. Populations studied so far are unique among the Simuliidae in that they exhibit chromosome structural monomorphism. These high resolution polytene chromosome maps will form the basis for future cytological characterization and phylogenetic comparisons amongst members of the feuerborni group展开更多
A new local cost function is proposed in this paper based on the linear relationship assumption between the values of the color components and the intensity component in each local image window,then a new quadratic ob...A new local cost function is proposed in this paper based on the linear relationship assumption between the values of the color components and the intensity component in each local image window,then a new quadratic objective function is derived from it and the globally optimal chrominance values can be computed by solving a sparse linear system of equations.Through the colorization experiments on various test images,it is confirmed that the colorized images obtained by our proposed method have more vivid colors and sharper boundaries than those obtained by the traditional method.The peak signal to noise ratio(PSNR) of the colorized images and the average estimation error of the chrominance values relative to the original images also show that our proposed method gives more precise estimation than the traditional method.展开更多
A new matting algorithm based on color distance and differential distance is proposed to deal with the problem that many matting methods perform poorly with complex natural images.The proposed method combines local sa...A new matting algorithm based on color distance and differential distance is proposed to deal with the problem that many matting methods perform poorly with complex natural images.The proposed method combines local sampling with global sampling to select foreground and background pairs for unknown pixels and then a new cost function is constructed based on color distance and differential distance to further optimize the selected sample pairs.Finally,a quadratic objective function is used based on matte Laplacian coming from KNN matting which is added with texture feature.Through experiments on various test images,it is confirmed that the results obtained by the proposed method are more accurate than those obtained by traditional methods.The four-error-metrics comparison on benchmark dataset among several algorithms also proves the effectiveness of the proposed method.展开更多
基金Supported by the Program from Ludong University(043312)~~
文摘[Objective]The study was to analyze the karyotype of Allium macrostemon Bunge in Laizhou City of Shandong Province.[ Method] The root tip of A. macrostemon was pretreated with 8-hydroxyquinoline solution, fixed, dissociated and stained for preparing the glass slide to reveal the chromosome number via the microscopic examination; the sparse cells with good chromosome morphology were photographed under microscope. [ Result ]Allium macrostemon Bunge in Laizhou City introduced in this study was tdploid; its somatic chromosome number was 24 and karyotype formula was K（2n） =3x=24m（2SAT） ＋ 1Bs, thus the karyotype belongs to 1A type. One of the chromosome No. 3 contained satellite, and chromosome deletion may be existed in one of the chromosome No. 5. In addition, B chromosome was observed in some cells.[ Conclusion] This introduction of triploid A. macrostemon found in China was the first time.
基金National Natural Science Foundation of China(No.61201412)
文摘In practical application,mean shift tracking algorithm is easy to generate tracking drift when the target and the background have similar color distribution.Based on the mean shift algorithm,a kind of background weaken weight is proposed in the paper firstly.Combining with the object center weight based on the kernel function,the problem of interference of the similar color background can be solved.And then,a model updating strategy is presented to improve the tracking robustness on the influence of occlusion,illumination,deformation and so on.With the test on the sequence of Tiger,the proposed approach provides better performance than the original mean shift tracking algorithm.
基金Supported by Demonstration and Promotion Project of Shanghai Municipal Committee of Agriculture[HNKT(2015)2-7]Jiangsu Agricultural"Three New Engineering"Project[SXGC(2017)208]Jiangsu Agricultural Science and Technology Innovation Fund[CX(15)1029]~~
文摘In order to develop green good, reduce the use of chemical fertilizer and pesticide, realize ＂double reduction＂ and synergism, improve safety and quality of products, protect the ecological environment and promote the healthy and steady development of strawberry industry, the goals and key technology of fertilizer-pesticide ＂double reduction＂ and synergism for greenhouse strawberry are summed up in this paper targeting at the current situation of strawberry production and combined with the new technology achievements at home and abroad. The key technology includes six items： healthy seedling cultivating technique, soil improvement and continuous cropping obstacle treatment technique, agricultural ecological prevention tech- nique, physicochemical trap of pest control technique, biological control technique and low residual risk chemical control technique.
文摘Though Yurman province contains some 562 known species of fish, no cell lines from any of these have been made available to date. To protect germplasm resources and provide an effective tool in solving problems at cellular level of Anabarilius grahami, a fish endemic to Fuxian Lake, Yunnan, China, we established and characterized the major features of a continuous cell line （AGF II） from the caudal fin tissue of A. grahami. This AGF II cell line consists of fibroblast-like cells and has been subeultured more than 60 times over the course of a year. The cell line was maintained in DMEM/F12 supplemented with 10% FBS, with a cellular doubling time of 51.1 h. We continued with more experiments to optimize the culture and storage conditions, and found a variety of interesting results： cells could grow at temperature between 24 ~C and 28 ~C, with the optimal temperature of 28 ~C. Likewise, the growth rate ofA. grahami fin cells increased when the FBS proportion increased from 5% to 20%, with the optimal growth at the concentrations of 20% FBS, cells were able to grow in L-15 and DMEM/FI2 with optimal growth at L-15; DMSO is a better eryoprotectant than Glycerol, EG and MeOH for AGFII cells with optimal concentration of 5% DMSO. Chromosome analysis also showed that the distribution of chromosome number varies from 38 to 52, with a modal peak at 48 chromosomes, accounting for 39.8% of all cells. Using the same primer pairs specific to mtDNA, the AGF II cell sequences obtained by PCR were identical to those from muscle tissues ofA. grahami. Both chromosome analysis and PCR amplification confirmed the AGF II cells were from A. grahami, also indicating that that current long-term artificial propagation ofA. grahami has been successful. Finally, we noted that when cells were transfected with pEYFP-N1 and pECFP-N1 plasmid, bright fluorescent signals were observed, suggesting that this cell line may be suitable for use in transfection and future gene expression studies.
基金Supported by Agricultural Seed Breeding Program of Shandong Province in 2014([2014]96)
文摘[Objective] This study was conducted to obtain tetraploid Pteroceltis tatar# nowii Maxim. with excellent ornamental traits. [Method] The stem apex growing points of Pteroceltis tatarinowii Maxim. were treated with different concentrations of colchicine solution for different hours to figure out a proper method and obtain poly- ploids. [Result] The most effective induction was obtained by treatment with 0.6%- 0.8% colchicine for 72 h with 34.2% mutation rate. Flow cytometry and chromosome observation of the stem apex growing point of P. tatarinowii Maxim. proved that the tetraploid plants were successfully obtained with chromosome number 2n=4x=36. [Conclusion] The result not only fills the blank of polyploid breeding of P. tatarinowii, but also provides an effective way to broaden the methods of cultivation of fast- growing, high-quality, disease-resilience, new varieties of Pteroceltis.
文摘The somaclone, C39, derived by tissue culture from the obligate apomict Paspalum dilatatum cv Raki (2n=50), had 50 chromosomes and a karyotype apparently identical to Raki.SC2 seedlings of C39 showed a high degree of phenotypic variation which was of ten associated with increased chromosome numbers, but some of the variant seedlings were karyotypically indistinguishable from Raki or C39. Plants with increased chromosome numbers exhibited a high degree of intraplant chromosome variation (aneusomaty). In one of the SC2seedlings, the chromosome number of root tip cells varied from 58 to 82 and in several other seedlings the range was more than 10. The results suggested that the ability to form seed apomictically was much reduced in C39 and that this plant showed some capacity for sexual reproduction and the resulting seedlings, with a chromosome number of about 70, were genetically unstable. Of 11 SC2 seedlings examined cytolog ically, 6 did not produce any viable seed. Seedlings grown from seed of the remaining 5 plants showed that aneusomaty persisted in the SC3 generation. SC3 seedlings which were phenotypically similar to their maternal parent showed a similar range of chromosome numbers to that parent. Some of the SC3 seedlings exhibited an even wider range of chromosome numbers (e.g.56-136), and these plants were all dwarfs.
基金Supported by the National Natural Science Foundation of China (No.30270235)
文摘We performed a karyotype analysis on four species from Phylum Nemertea using regenerating somatic cells. Two palaeonemertean species, Cephalothrix hongkongiensis and Cephalothrix sp., had the same chromosome number （2n=28）, but different karyotypes （16 m＋10 sm＋2 st, NF=54 and 22 m＋4 sm＋2 st, NF=54, respectively）. The karyotypes of the two heteronemerteans, Notospermus geniculatus and Ramphogordius sanguineus were 2n=38, 30 m＋6 sm＋2 sm/st （NF=76/74） and 2n=56, 48 m＋4 sm＋4 sin/st CNF=112/108）, respectively.
基金The project was supported by the National Natural Science Foundation of China(30370135)
文摘The present paper deals with the genus Willowsia and its Chinese species. A new species, Willowsia guangxiensis, sp. nov., is described from Guangxi of South China.
基金the Natural Science Foundation(Grant No.51178463)the Fundamental Research Funds for the Central Universities(10CX04018A)of China for financial support of this studythe Environment and Safety Technology Center of China University of Petroleum for its technical and logistical assistance
文摘For dealing with high-salinity wastewater in the refinery, the high cost of driving heat source is the disadvantage of multi-effect distillation (MED) so it is of great importance to evaluate the performance of low-temperature heat source for conducting MED and select the optimal temperature for it. Both the MED and the low-temperature heat sources studied in this paper were from a typical refinery located in northwestern China. Besides, a new methodology to evaluate heat sources as the optimal candidate was proposed for MED based on the grey system theory. Five process units, which included 18 fluids of the refinery, were named as the evaluation projects. Three factors, which included safety effects, total costs and characteristics of low-temperature heat sources were determined as the evaluation indexes, the values of which were established through the analyses. The results obtained through the grey correlation analyses have revealed that the grey correlation degrees of these units were 0.661(AVDU), 0.732 (#1 FCCU), 0.618 (#2 FCCU), 0.535 (#1 DCU), and 0.572 (#2 DCU), respectively. Thus, the optimal heat source was provided from #1 FCCU. Through further analyses of the fluids from #1 FCCU, the grey correlation degrees of the fluids were 0.597 (oil and gas at top of tower), 0.714 (recycle oil and gas), and 0.512 (diesel), respectively. Thus, the optimal heat source was the oil and gas recycle stream.
基金Supported by the Knowledge Innovation Program of Chinese Academy of Sciences (Nos.KZCX2-YW-213,and O72715)
文摘This paper reports the occurrence of a marine brachyuran crab species Eucrate alcocki Serene, in Serene et al., 1973, of the family Euryplacidae Stimpson, 1871, for first time from India, based on a male specimen from Parangipettai fish landing centre in Bay of Bengal, Southeast Coast of India. Although morphologically corresponding with what is currently defined as E. alcocki, the color pattern of the carapace of the present specimen is rather different from that of the Chinese material-only the anterior fifth of the carapace is marked with scattered red spots, the rest of the surface is yellowish, with four unusually shaped red blotches which almost look like Sanskrit characters.
文摘High quality polytene chromosome maps （n=3） of a Himalayan Simuliid Simulium praelargum Datta, 1973 are presented and represent the first cytological description of a taxon found in the feuerborni group, subgenus Nevermannia. Polytene chromosomes one （I） and two （II） are metacentric, chromosome three （III） is submetacentric with the length of each chromosome occupying 37.25 %, 31.36 % and 31.34 % of the total complement length, respectively. Typical simuliid diagnostic intergeneric chromosomal markers are found within the polytene complement of this species. The nucleolar organizer （N.O.） is found at the base of the short arm of chromosome one （IS）, the Ring of Balbiani （R.B.）, double bubble （D.B.） and triad occur in the short arm of chromosome two （IIS）, the Parabalbiani Ring （EB.） and grey band （gb） occur in the long arm of chromosome two （IIL） and the Blister （BL） and Capsule （Ca） occur in the short arm of chromosome three （IIIS）.Terminal bands at the end of IIIS are heterochromatinized and present atypically with respect to other simuliid fauna. Populations studied so far are unique among the Simuliidae in that they exhibit chromosome structural monomorphism. These high resolution polytene chromosome maps will form the basis for future cytological characterization and phylogenetic comparisons amongst members of the feuerborni group
基金Supported by the National Natural Science Foundation of China(No.61073089)the Joint Funds of the National Natural Science,Foundation of China(No.U1304616)the Qinhuangdao Research&Development Program of Science&Technology(No.2012021A044)
文摘A new local cost function is proposed in this paper based on the linear relationship assumption between the values of the color components and the intensity component in each local image window,then a new quadratic objective function is derived from it and the globally optimal chrominance values can be computed by solving a sparse linear system of equations.Through the colorization experiments on various test images,it is confirmed that the colorized images obtained by our proposed method have more vivid colors and sharper boundaries than those obtained by the traditional method.The peak signal to noise ratio(PSNR) of the colorized images and the average estimation error of the chrominance values relative to the original images also show that our proposed method gives more precise estimation than the traditional method.
基金Supported by the National Natural Science Foundation of China(No.61133009,U1304616)
文摘A new matting algorithm based on color distance and differential distance is proposed to deal with the problem that many matting methods perform poorly with complex natural images.The proposed method combines local sampling with global sampling to select foreground and background pairs for unknown pixels and then a new cost function is constructed based on color distance and differential distance to further optimize the selected sample pairs.Finally,a quadratic objective function is used based on matte Laplacian coming from KNN matting which is added with texture feature.Through experiments on various test images,it is confirmed that the results obtained by the proposed method are more accurate than those obtained by traditional methods.The four-error-metrics comparison on benchmark dataset among several algorithms also proves the effectiveness of the proposed method.
