焦页XX-HF井近钻头仪器“落鱼”打捞技术

涪陵页岩气二期开发过程中,为了能够及时发现地层变化,钻井平台部署的第一口井在三开井段一般使用近钻头仪器,以便及时调整井眼轨迹,提高优质页岩的钻遇率。动力钻具前面安装近钻头仪器和钻头,导致动力钻具传动轴负载增加,传动轴易发生疲劳破坏。焦页XX-HF井钻进至3971.42 m处,近钻头仪器无信号,起钻后发现动力钻具传动轴断裂,近钻头仪器和钻头落井。本井“落鱼”较短且处于水平段,“鱼头”难找,起下钻摩阻大,不易通过遇阻判断打捞工具是否接触“鱼顶”。通过对孔内情况仔细分析,制定可行的打捞方案,现场加工母锥打捞工具,最终成功将井下“落鱼”安全捞出。本井所采取的处理思路和技术措施对今后同类事故的处理具有一定的借鉴意义。

FN-1井复杂条件下深井侧钻技术分析

以塔里木油田FN - 1探井为例 ,阐述深井复杂地层侧钻的 2种工艺方法 ,即初级分支钻进和高级分支定向钻进 ,分析对比 2种工艺方法的优缺点 。

LXLT-B型连续油管打捞筒

31.75的连续油管具有外径小、壁薄、挠曲性好等特性。如果断落到井里 ,其打捞非常困难。在卡瓦打捞筒的基础上改进设计出LXLT -B型连续油管打捞筒。介绍其结构原理和现场试验情况。

Comparison of intestinal bacterial communities in grass carp,Ctenopharyngodon idellus,from two different habitats

The intestinal bacteria of vertebrates form a close relationship with their host.External and internal conditions of the host,including its habitat,affect the intestinal bacterial community.Similarly,the intestinal bacterial community can,in turn,influence the host,particularly with respect to disease resistance.We compared the intestinal bacterial communities of grass carp that were collected from farm-ponds or a lake.We conducted denaturing gradient gel electrophoresis of amplified 16S rRNA genes,from which 66 different operational taxonomic units were identified.Using both the unweighted pair-group method with arithmetic means clustering and principal component analysis ordination,we found that the intestinal bacterial communities from the two groups of pond fish were clustered together and inset into the clusters of wild fish,except for DF-7,and there was no significant correlation between genetic diversity of grass carp and their intestinal bacterial communities(Mantel one-tailed test,R=0.157,P=0.175).Cetobacterium appeared more frequently in the intestine of grass carp collected from pond.A more thorough understanding of the role played by intestinal microbiota on fish health would be of considerable benefit to the aquaculture industry.

A Comparative Study of Fish Assemblages Near Aquaculture, Artificial and Natural Habitats

Habitat plays a critical role in regulating fish community structure. Using the data collected from a monthly trammel net survey in Ma'an archipelago off the east coast of China, we evaluated impacts of five different habitats(artificial reefs, mussel farms, cage aquaculture, rocky reefs and soft bottom) on fish assemblages. This study suggests that artificial reefs(AR) have significantly higher species richness, abundance and diversity than mussel farms(MF) or soft bottom(SB) habitats during most seasons, and that fish taxa in the AR habitats are similar to those in the rocky reef(RR) habitats. Two different fish assemblage patterns were revealed in the study area using non-metric multidimensional scaling ordination: an assemblage dominated by reef fishes(especially by Scorpaenidae species) in AR, RR and cage aquaculture(CA) habitats and an assemblage dominated by Sciaenidae species in MF and SB habitats. We suggest that reef fishes play a key role in differentiating fish community structures in the study area. Although few differences in fish abundance and diversity were found between the CA and SB habitats, a more diverse age structure was observed in the CA habitats. A much more complex fish assemblage and enhanced population of local species were established as a result of the presence of both floating and fixed artificial structures, probably through improved survival rates.

Fish community diversity in the middle continental shelf of the East China Sea

The status of fishery stocks in the coastal waters of China is far from ideal, mainly due to climate change and the impacts of human activities （e.g., pollution and overfishing）. Thus, the restoration and protection of fishery resources have become critical and complex. The stability and balanced structure of the fish community is a basic foundation for the protection of fishery resources. Based on data collected from bottom trawls by the R/V Beidou in continental shelf of the East China Sea in November 2006 and February 2007, changes in the composition and diversity of fish species and functional groups were analyzed. The research area was divided into offshore waters and inshore waters by the two-way indicator species analysis （TWIA）. The results showed that the dominant species were different between offshore waters and inshore waters and also varied with the survey time. The most abundant family was Sciaenidae and Teraponidae in November 2006, Sciaenidae, Engraulidae and Triglidae were most abundant in February 2007. The species belonged mainly to mobile piscivores （G6）, benthivores/piscivores （G4）, benthivores （G3） and planktivores （G1）, and the dominant species in November 2006 were commercial species （e.g. Larimichthys polyactis and Trichiurusjaponicus）, but small-sized species were dominant in February 2007 （e.g., Harpadon nehereus, Benthosema pterotum, Champsodon capensis, and Acropoma japonieum）. The species diversity showed a similar trend as the functional group diversity. Stations with higher diversity were mainly distributed in inshore waters in February 2007, whereas higher diversity was found in offshore waters in November 2006. The highest biomass and species number were found in G6 group, followed by the G4, G5 and GI groups. The distribution of the number of individuals of each functional group showed the opposite trend as that of the biomass distribution. In addition, the size spectra were mainly concentrated around 3-29 cm, and the individual number of fish species gradually decreased with the increase in body size, but the relative biomass showed a moderate fluctuation in each size class. These changes showed that species with faster growth rate and earlier maturity age became dominant in continental shelf of the East China Sea. So the variations in biological characteristics of fish should be fully considered in maintaining sustainable utilization of fishery resource.

Community structure of planktonic copepods in artificial reefs of Wenchang,Hainan

To understand the characteristics of population structure of planktonic copepods and their response mechanisms to marine environmental factors in different seasons,the community structure of planktonic copepods in samples obtained from 7 stations in artificial reefs of Wenchang,Hainan Province in March and September 2019 were investigated.A total of 33 species of planktonic copepods belonging to 23 genera within 17 families were identified in artificial reefs,with 27 species and 21 genera in spring,and 23 species and 19 genera in fall,respectively.The identified planktonic copepods were further categorized into estuarine groups,warm temperate groups,warm water coastal groups,and warm water widespread groups.The predominant species in spring were mainly composed of Temora turbinata and Canthocalanus pauper,whereas Tortanus gracilis and Subeucalanus subcrassus were dominant species in fall.The species and abundance of planktonic copepods in spring were higher than that in fall,while the diversity index and evenness were vice versa,with obvious seasonal differences.In addition,there was a correlation between the population of copepods and physicochemical environmental factors.The major warm water group of copepods in spring showed different degree of correlation with environmental factors,such as NO2-N,NH3-N,pH,water depth,temperature etc.,while the population of copepods in fall was affected by limiting factors such as dissolved oxygen,pH,and temperature.In conclusion,copepods species inartificial reefs were rich and diverse,and their community structure tended to be stable.In addition,the surrounding water quality was generally good,and the whole marine ecosystem and ecological environment were in good condition.

Influence of a large dam on the longitudinal patterns of fish assem- blages in Qingyi Stream

Using seasonally collected data(2009-2010) from 15 sampling sites that represent first- to fifth-order streams within the Qingyi watershed,we examined the spatio-temporal patterns of fish assemblages along two longitudinal gradients to explore the effects of a large dam on fish assemblages at the watershed scale.No significant variation was observed in either species richness or assemblage structure across seasons.Species richness significantly varied according to stream order and gradient.Dam construction appeared to decrease species richness upstream substantially,while a significant decrease between gradients only occurred within fourth-order streams.Along the gradient without the large dam,fish assemblage structures presented distinct separation between two neighboring stream orders,with the exception of fourth-order versus fifth-order streams.However,the gradient disrupted by a large dam displayed the opposite pattern in the spatial variation of fish assemblages related with stream orders.Significant between-gradient differences in fish assemblage structures were only observed within fourth-order streams.Species distributions were determined by local habitat environmental factors,including elevation,substrate,water depth,current discharge,wetted width,and conductivity.Our results suggested that dam construction might alter the longitudinal pattern in fish species richness and assemblage structure in Qingyi Stream,despite the localized nature of the ecological effect of dams.

Status and historical changes in the fish community in Erhai Lake

Erhai Lake is the second largest freshwater lake on the Yunnan Plateau, Southwest China. In recent decades, a number of exotic fish species have been introduced into the lake and the fish community has changed considerably. We evaluated the status of the fish community based on surveys with multimesh gillnet, trap net, and benthic fyke-net between May 2009 and April 2012. In addition, we evaluated the change in the community using historical data （1952-2010） describing the fish community and fishery harvest. The current fish community is dominated by small-sized fishes, including Pseudorasbora parva, Rhinogobius giurinus, Micropercops swinhonis, Hemiculter leucisculus, and Rhinogobius cliffordpopei. These accounted for 87.7% of the 22 546 total specimens collected. Omnivorous and carnivorous species dominated the community. A canonical correspondence analysis （CCA） plot revealed that the distribution of fishes in the lake is influenced by aquatic plants, water temperature, pH, and season. The abundance of indigenous species has declined sharply, and a majority of endemic species have been extirpated from the lake （a decrease from seven to two species）. In contrast, the number of exotic species has increased since the 1960s to a total of 22 at present. The fishery harvest decreased initially following the 1960s, but has since increased due to the introduction of non-native fish and stocking of native fish. The fishery harvest was significantly correlated with total nitrogen, not total phosphorus, during the past 20 years. Based on our results, we discuss recommendations for the restoration and conservation of the fish resources in Erhai Lake.

Status of two Coreius species in the Three Gorges Reservoir,China

Dam construction alters natural flow regimes which,in turn,cause significant changes in fish communities during and after impoundment. The construction of the Three Gorges Reservoir,from impoundment of the Changjiang(Yangtze) River,China,may have affected native fish species. Thus,the status of two lotic freshwater fish species,Coreius heterodon and C.guichenoti,were monitored in the Three Gorges Reservoir,including fish abundance,individual composition,growth,condition,and mortality. Data on both species were gathered from upstream,midstream and downstream areas of the reservoir and,where available,from studies published before and after dam construction. Lower abundance,slower growth,a less diversified age structure,poorer fish condition(indicated by hepatosomatic index) and higher mortalities were recorded in sites nearest the dam compared with upstream areas. Furthermore,after final impoundment,individual Coreius species inhabiting the area changed,with young individuals becoming more abundant,while upstream of the reservoir the two Coreius species became smaller at a given age. The results show that the status of the two Coreius species was subject to dramatic changes after impoundment.

Composition of fish communities in an intertidal salt marsh creek in the Changjiang River estuary,China

Fish communities in a （third-order） intertidal creek in Dongtan marsh in the Changjiang （Yangtze） River estuary were investigated seasonally for one year. A total of 1 996 fish specimens （10 967.8 g） comprising 26 species and 15 families were collected. Abundances of fish communities in the intertidal salt marsh creek were primarily dominated by Boleophthalmus pectinirostris （19.8%）, Collichthys lucidus （18.6%）, Periophthalmus magnuspinnatus （18.2%）, Liza haematocheilus （17.9%）, and secondarily by Mugilogobius abel （8.5%）, L. carinatus （7.2%）, Odontamblyopus lacepedii （4.3%）, and Acanthogobius ommaturus （3.9%）; another 18 species were present only occasionally. Non-MDS ordination and SIMPER analysis indicated that there were two fish communities in the intertidal salt marsh creek. In spring, the communities were dominated by B. pectinirostris, P. magnuspinnatus, C. lucidus and M. abei; in summer, autumn, and winter by L. haematocheilus, L. carinatus, A. ommaturus and O. lacepedii. Some species showed strong habitat selection; L. carinatus and P magnuspinnatus were distributed mainly in the upper and middle creek, while B. pectinirostris, M. abei and O. lacepedii inhabited the middle and lower creek. The study indicated that the salt marshes of the Changjiang River estuary are an important nursery and feeding habitat for many fishes and should be protected.

The impact of geomorphology of marsh creeks on fish assemblage in Changjiang River estuary

Tidal marshes are an important habitat and nursery area for fi sh.In the past few decades,rapid economic development in the coastal areas of China has led to the interruption and destruction of an increasing number of tidal marshes.The growing interest in tidal marsh restoration has increased the need to understand the relationship between geomorphological features and fi sh assemblages in the design of marsh restoration projects.We studied temporal variations in,and the effects of creek geomorphological features on,the estuarine tidal creek fi sh community.Using modifi ed channel nets,we sampled fi sh monthly from March 2007 to February 2008 from seven tidal creeks along an intertidal channel system in Chongming Dongtan National Nature Reserve.Fourteen creek geomorphological variables were measured or derived to characterize intertidal creek geomorphological features.The Gobiidae,with 10 species,was the most speciesrich family.The most abundant fi sh species were Liza affi nis,Chelon haematocheilus,and Lateolabrax maculatus.The fi sh community was dominated by juvenile marine transients,which comprised about 80% of the total catch.The highest abundance of fi sh occurred in June and July,and the highest biomass occurred in December.Canonical redundancy analyses demonstrated that depth,steepness,cross-sectional area,and volume signifi cantly affected the fi sh species assemblage.L.affi nis favored small creeks with high elevations.Synechogobius ommaturus,Acanthogobius luridus,and Carassius auratus preferred deep,steep creeks with a large cross-sectional area and volume.These fi ndings indicate that the geomorphological features of tidal creeks should be considered in the conservation and sustainable management of fi sh species and in the restoration of salt marshes.

Characterization of dominant and cellulolytic bacterial communities along the gut of silver carp Hypophthalmichthys molitrix during cyanobacterial blooms

Silver carp is one of the most important planktivorous fish in Chinese aquaculture and plays a significant role controlling cyanobacterial blooms. A balanced gut microbiota is crucial for growth and health of the host because of its important roles in immune defense, digestion of complex carbohydrates, and production of enterocytes. In our study, the dominant bacterial and cellulolytic bacterial （Clostridium 1, Clostridium 111, Clostridium XIVab, and Fibrobacter） communities in the contents and mucus of the silver carp gut （foregut, midgut, and hindgut） were analyzed by denaturing gradient gel electrophoresis and quantitative polymerase chain reaction （qPCR） analyses. The results revealed that the dominant and cellulolytic bacterial communities were significantly different among gut regions as well as in contents and mucus. Bacterial diversity and richness in contents and mucus increased along the gut and were higher in contents than those in local mucus. A sequence analysis of gut samples exhibited the conservative phylotypes of Proteobacteria, Actinobacteria, and Firmicutes. The gut of silver carp harbored an abundance of cellulolytic bacteria, particularly Clostridium XIVab. The foregut segment had the highest proportions of the four cellulolytic bacteria, followed by the midgut and hindgut. However, the proportions of cellulolytic species in the silver carp gut was much lower than those in the terrestrial vertebrate gastrointestinal tract. We conclude that gut bacteria could help silver carp obtain energy from cyanobacteria, which may be why silver carp can maintain high growth rates during cyanobacterial blooms.

Spatial and seasonal variations in the trophic spectrum of demersal fish assemblages in Jiaozhou Bay, China

Trophic structure of fish communities is fundamental for ecosystem-based fisheries management, and trophic spectrum classifies fishes by their positions in food web, which provides a simple summary on the trophic structure and ecosystem function. In this study, both fish biomass and abundance trophic spectra were constructed to study the spatial and seasonal variations in the trophic structure of demersal fish assemblages in Jiaozhou Bay, China. Data were collected from four seasonal bottom trawl surveys in Jiaozhou Bay from February to November in 2011. Trophic levels(TLs) of fishes were determined by nitrogen stable isotope analysis. This study indicated that most of these trophic spectra had a single peak at trophic level(TL) of 3.4–3.7, suggesting that demersal fish assemblages of Jiaozhou Bay were dominated by secondary consumers(eg. Pholis fangi and Amblychaeturichthys hexanema). The spatial and seasonal variations of trophic spectra of Jiaozhou Bay reflected the influence of fish reproduction, fishing pressure and migration of fishes. Two-way analysis of variance(ANOVA) showed that seasonal variations in trophic spectra in Jiaozhou Bay were significant(P <0.05), but variations among different areas were not significant( P >0.05). The trophic spectrum has been proved to be a useful tool to monitor the trophic structure of fish assemblages. This study highlighted the comprehensive application of fish biomass and abundance trophic spectra in the study on trophic structure of fish assemblages.

The characteristic of phytoplankton community and its variation at artificial reef area of the coastal sea of Tianjin

Three cruises were investigated respectively in May,July and November 2016 in the artificial reef area of 2010,2012,and 2014 and the non-artificial reef area(control area)in the coastal area of Tianjin.Each area had 3 sampling stations,a total of 12 sites.The result showed that 58 taxa of phytoplankton were identified which belongs to 2 phyla 28 genera,Among these species,bacillariophyta including 19 genera 44 species,pyrrhophyta including 9 genera and 14 species.Give priority to with diatom species,accounting for 75.9%of the total species,followed by dinoflagellates,accounting for 24.1%.Dominant phytoplankton species give priority to with coscinodiscus and Chaetoceros of bacillariophyta,mainly including Coscinodiscus wailesii,C.asteromphalus,C.granii,C.oculus-iridis,C.spp.,Chaetoceros castracanei,C.lorenzianus,C.curvisetus,Pseudo-nitzschiapungens,Guianardia delicatula,Rhizosolenia setigera,Proboscia alata f.indica and Noctilluca scintillans.Phytoplankton cell abundance revealed obviously seasonal changes,the average cell abundance respectively was 94.79×10^4 cells/m^4、39.53×10^4 cells/m^4 and 21.5×10^4 cells/m^3.Phytoplankton diversity index has obviously seasonal variation,and in November,shannon-wiener diversity index,Margalef index and Pielou index are higher than in May and July.Comparing the artificial reef areas with the control area,the phytoplankton cells abundance of the control area in May was significantly higher than the artificial reef areas.In July and November,the artificial reef area of 2014 phytoplankton cell abundance was significantly higher than other artificial reef area and the control area.Diversity index of phytoplankton in the control area and the artificial reef of 2012 was lower than the artificial reef area of 2010 and 2014,and lowest in non-artificial reef areas.Obviously,the construction of artificial reef had significant effect for the improvement of the phytoplankton community diversity,but the phytoplankton community diversity is not always increasing and fluctuating to a certain extent as time goes by.

Do physicochemical variables regulate the distribution of zooplankton communities in reservoirs dominated by filter-feeding carp?

The temporal and spatial distributions of zooplankton communities in the upper, middle, and lower reaches of the Xin＇anjiang Reservoir, Zhejiang, China, were investigated monthly, between 2009 and 2010. Silver carp （Hypophthalmichthys moBtrix） and bighead carp （Aristichthys nobilis） dominated the pelagic fish community of this large, deep reservoir. Cladocerans were distributed evenly throughout the reservoir. Rotifers were mainly found in the upper reaches, while copepods tended to assemble in the lower reaches. The Pearson correlation analysis and stepwise multiple regression were used to identify the major physicochemical gradients influencing community variations. Zooplankton community distributions were influenced by water temperature, dissolved oxygen, phosphorus, nitrogen, and silicon. Excess nutrients, in particular silicon, stimulated rotifer growth. Based on these findings, it is possible to use rotifer density as a bioindicator of eutrophic status in deep reservoir ecosystems.

Effects of Channelization on Fish Biomass in River Ecosystems

Channel straightening in a naturally meandering river is a common historical trigger of channel incision which typically results in stream bank destabilization. Several of the larger river systems in the upland portion of the Yazoo River Basin have subjected channelization resulting in profound changes in the physical and geomorphological characteristics of these systems. Fish were sampled using electroshocking gear and hoop nets to evaluate the impact of stream bank destabilization and loss of habitat heterogeneity resulting from channelization on fish communities. While distinct differences in the fish communities were evident, only the Skuna appeared to have characteristics of a damaged system. More than 95% of the biomass was comprised of species reaching an adult length of less than 300 mm. The lotic omnivorous fishes that dominated the biomass from Skuna are often associated with smaller streams rather than rivers. Furthermore, 72% of the catch consisted of fish preferring littoral zone habitats. The shallow depth and lack of woody debris in Skuna provided a selective advantage for smaller species of fish that could use shoreline habitats as protection from the current. Based on results from the Skuna River, channel straightening that leads to channel incision, bank failure and over widening provide habitats too shallow to support a community of fishes typical of northern Mississippi riverine system. This information may be useful in making comparison of damaged riverine ecosystems and assist managers in determining impairment and success in the TMDL （Total Maximum Daily Load） process.

Seasonal variation in species composition and abundance of demersal fish and invertebrates in a Seagrass Natural Reserve on the eastern coast of the Shandong Peninsula,China

Seagrass habitats are structurally complex ecosystems, which support high productivity and biodiversity. In temperate systems the density of seagrass may change seasonally, and this may influence the associated fish and invertebrate community. Little is known about the role of seagrass beds as possible nursery areas for fish and invertebrates in China. To study the functioning of a seagrass habitat in northern China, demersal fish and invertebrates were collected monthly using traps, from February 2009 to January 2010. The density, leaf length and biomass of the dominant seagrass Zostera marina and water temperature were also measured. The study was conducted in a Seagrass Natural Reserve(SNR) on the eastern coast of the Shandong Peninsula, China. A total of 22 fish species and five invertebrate species were recorded over the year. The dominant fish species were Synechogobius ommaturus, Sebastes schlegelii, Pholis fangi, Pagrus major and Hexagrammos otakii and these species accounted for 87% of the total number of fish. The dominant invertebrate species were Charybdis japonica and Octopus variabilis and these accounted for 98% of the total abundance of invertebrates. There was high temporal variation in species composition and abundance. The peak number of fish species occurred in August–October 2009, while the number of individual fish and biomass was highest during November 2009. Invertebrate numbers and biomass was highest in March, April, July and September 2009. Temporal changes in species abundance of fishes and invertebrates corresponded with changes in the shoot density and leaf length of the seagrass, Zostera marina.

Fish Community Structure and Habitat Preference in Hinval Freshwater Stream of Garhwal Himalayas, India

Fish diversity in different habitats and their preference for a particular habitat was studied in a freshwater stream Hinval in Western Himalayas of Garhwal region. A total of 7 fish species belonging to 2 orders were recorded. The maximum fish diversity and dominance was reported in the cyprinids. Two species of order Cypriniformes, Tot putitora （Ham.） and Barilius bendelisis （Ham.） were recorded at all the three sites, although they were more abundant at sites I and II. Among the Barilius spp., Barilius bendelisis （Ham.） was the most common at all the study sites, while Barilius barna （Ham.） and Barilius vagra （Ham.） were only found at Ⅰand Ⅱsites. The pool habitat was the most preferred habitat of almost all the species with （＇H＇=0.521-0.594） tbllowed by rapid and riffle habitat respectively.