怎样区分“资格”与“资质”

据可锐职业顾问一项调查显示，有7成以上经验丰富的“职场老人”无法晋升，遭遇职业的7年之痒，与之相反，工作经验在5年左右，有近5成人可以顺利进入企业高层。为什么会出现如此之大的反差？可锐通过实际案例剖析其中奥秘。

AFLP Fingerprint and SCAR Marker of Watermelon Core Collection

The identification of germplasm is an important step for effective utilization of the available germplasm. In previous studies, isoenzyme, RAPD and SSR techniques had been used to conduct the genetic identification of watermelon ( Citrullus lanatus (Thunb.) Mansf.), but their effectiveness was limited due to the extremely narrow genetic background among watermelon genotypes. In this research, amplified fragment length polymorphism (AFLP), which was reported as a reliable technique with high efficiency in detecting polymorphism, was used to conduct genetic analysis and variety identification of thirty genotypes of watermelon core collection that represent a wide range of breeding and commercially available germplasm. As a result, a DNA fingerprint based on 15 bands amplified with four primer combinations was developed. In this fingerprint, each genotype has its unique fingerprint pattern and can be distinguished from each other. Furthermore, in or der to facilitate the utilization of AFLP marker in practice, one specific AFLP band of genotype 'PI296341' coming from fragment amplified by primer combination E-AT/M-CAT was successfully converted into a sequence characterized amplified region (SCAR) marker.

Study on Present Status of Plant Germplasm Resources Conservation and Countermeasures

Plant germplasm resources is the material foundation and key point for agricultural production, crop breeding and bioengineering. China is one of the centers for the origin of the cultivated plants worldwide, as well as one of the countries with most abundant plant resources and the highest biodiversity. Strengthening research in the conservation and utilization of plant germplasm resources is of significant value and importance. In this study, the present status of conservation, utilization, and existing problems of plant germplasm resources in China and around the world were reviewed; further, through analyzing measures taken by global countries for plant germplasm resources protection, countermeasures for plant germplasm resources conservation in China were also presented from three aspects, namely, collection and conservation, information network construction and national legislation and policies.

Genetic Diversity of Achnatherum Splendens

[Objective] Study on the genetic diversities of A.splendens from different areas of xinjiang. [Method] The genetic diversities among seven populations and within two populations were analyzed by RAPD. [Result] Genetic clustering results presented that the relationships among populations of A. splendens are directly related with geographic positions, and within population are related with habitats. [Conclusion] RAPD technique can be used to study genetic diversity of A. splendens.

SSR-based Analysis on the Genetic Relationship of 21 Maize Germplasms in Guizhou Province

To reveal the genetic diversity of maize germplasm resources in Guizhou Province, we screened 20 pairs of SSR primers out of 100 pairs tested for amplify-ing the al eles in 21 shares of maize germplasm col ected there. Total y 85 al eles were identified from the experimental materials, and each primer pair amplified 2-9 al eles, with an average of 4.25. The polymorphism information content （PIC） values varied from 0.19 to 0.74, with an average of 0.50. The clustering analysis by UPG-MA indicated that the genetic distance among the tested lines was 0.22-0.63, with an average of 0.43. This provided the theoretical foundations for establishing the finger print chromatogram of maize, further for exploring and utilizing maize germplasms Guizhou Province.

Research on Background and Utilization of Germplasm Resources in Maize

[Objective] The research aimed at introducing the background and utilization of germplasm resources in maize.[Method] The research progress on genetic background and utilization of maize germplasm resources was comprehensively reviewed in this paper,including maize heterotic groups and heterotic patterns,their flexible application in breeding and the utilization of various types of original materials.[Result] The results show that germplasm resource is the premise and basis for maize breeding.In-depth understanding and a reasonable selection of germplasm resources can significantly improve the breeding efficiency and speed up the breeding process.At the same time,it is considered that the selection and construction of original materials in maize breeding can not be limited to the heterotic groups and heterotic patterns,but has to be used reasonablely according to different types of germplasm resources and individual breeding objective and idea as well.[Conclusion] This study had broadened the idea and provided inspiration for the maize breeding practice in China.

ISSR Analysis of Genetic Relationship of Germplasm Resource in Prunus mume Sibe. et Zucc

Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120 fragments were amplified, of which 98 were polymorphic loci, accounting for 81.67% of total. Tested materials were divided into 3 classes, as was fundamentally accorded with the traditional classification base on horticulture. There was no obvious difference in geographic relationship among the clustering results.

Research Progress on Germplasm Resources of Common Bean

The origin, classification, utilization and evaluation of germplasm resources, and breeding methods were introduced. The researches of biotic or/and abiotic stress, exploration of genomics and proteomics in common bean in recent years were reviewed in this paper, in order to provide theoretical reference for utilization and innovation of germplasm resources in common bean.

Quality and change analysis of forest resource in typical Changbai Mountain forest region

The utilization and changes of forest resources were studied in the Lishuihe Forest Bureau. Based on remote sensing images in 1985 and 1999, changes of major forest resources were analyzed by statistical and overlap method and classified quantitatively. The results showed that in recent 15 years, logging spots and man-made young forest changed violently, which was due to human activities. Different forest management manners and harvesting intensity played an important role in forest resources change. Dongsheng and Xilinhe tree farms were typical cases of different forest status and management for the Bu-reau, where forest succession was intervened by either human or natural disturbance. Dongsheng Tree Farm underwent a light harvest intensity and maintained a unit stock volume of 536.27 m3hm-2, as much as that of broadleaf/Korean pine forest of Changbai Mountain Natural Reserve; Xilinhe Tree Farm underwent an intense harvest and was composed of secondary forests, where mature forests just had a small percentage and the unit stock volume was low. The study was useful to guide future forest management. What抯 more, problems found in the research were also analyzed and reasonable advice was given to the local forest management.

Subcellular Distribution of Cadmium in Mining Ecotype Sedum alfredii

The mining ecotype Sedum alfredii Hance could tolerate and grow normally in a nutritive solution containing cadmium (Cd) as high as 400 mumol/L. Under such a high Cd concentration, the subcellular accumulation of Cd in root, stem and leaf of this plant was found to be the highest in the cell wall, less in the soluble fraction and lowest in the cell organs. The mode of subcellular distribution of Cd in the mining ecotype S. alfredii was similar to other hyper accumulators of heavy metals, in which Cd was distributed more in the aerial part of plant. The results suggest that the mining ecotype S. alfredii is a new species of Cd hyperaccumulator.

Collection and Preservation of Cashew Germplasms Resources Research Progress in China

The cashew tree is a typical tropical nut tree species in China, and the collection, identification and innovated utilization of excellent cashew germplasm resources are of great significance to increase the income of farmers and agricultural benefits in the tropical area of China. This study summarized the major advances on cashew germplasm resources in China, analyzed the major research progress and existing problems from the aspects of the collection and evaluation, resistances, genetic diversity of cashew germplasm resources, and put forward the work ideas to analyze the construction of core germplasm bank and the genetic stability of the major biological characteristics of cashew germplasm resources, which were of important significance for the improvement of the cultivar classification and resource evaluation system of cashew germplasm resources in China.

Establishment and Share of Tea Germplasm Database of Yunnan Province

[Objective] The paper was to establish tea germplasm database of Yunnan Province,and promote sharing of the tea germplasm resources.[Method] Eight hundred and thirty copies of tea germplasm resources of Yunnan Province were first systematically documented by using Access database software,the generic description of 631 tea resources and characteristic description of 300 tea resources were submitted for e-platform,then linked with the national e-platform for natural scientific and technological resources,and the tea germplasm database of Yunnan Province was established.[Result] Based on the conservation and utilization status of tea germplam resources,the sharing and utilization framework of tea germplam resources was presented.Many problems and suggestion about tea germplasm resources in the process of conservation,documentation concordance and sharing were pointed out.For example,conservation areas were separated and system was not completed;the main traits assessment and identification researching work had not completely accomplished and sharing was inefficient.[Conclusion] The paper laid foundation for standardized,digitized and information-based management of tea germplasm resources.

Evaluation and Screening of Aceraceae Germplasm Resources

[Objective] The aim was to screen out excellent Aceraceae germplasm resources which can be applied in gardens and urban landscape. [Method] The set of 17 biological traits of 24 Aceraceae species were observed and recorded. Taking into consideration aesthetic evaluation of different groups of people, the studied species were graded in quantitative scale. The species with higher overall score is considered better germplasm resource. [Result] The Aceraceae species with top ten highest overall scores were： Acer japonicum ＇Aconitifolium＇ （45.5）, Acer palmatum var. thunbergii （43.8）, Acer palmatum （41.8）, Acer elegantulum （39.2）, Acer elegantulum var. macrurum （39.2）, Acer rubrum （39.1）, Acer Cordatum（38.8）, Acer truncatum （38.3）, Acer olivaceum （37.7） and Acer acutum （37.3）. [Conclusion] The above ten species can be used preferentially as excellent Aceraceae germplasm resources for landscaping.

Genetic Diversity Analysis of 44 Shares of Hibiscus cannabinus L. Germplasm Resources Using ISSR Molecular Marker

[Objective] This study was to reveal the genetic diversity and genetic relationship of the kenaf(Hibiscus cannabinus L.) resources from different origins, thus providing basis for genetic improvement and molecular marker-assisted breeding of kenaf. [Method] Ninety one ISSR molecular markers were used for amplification on 44 shares of kenaf germplasm resources, of which 21 showing good diversity and clear bands were chosen for PCR amplification. Based on amplification results, the genetic similarity coefficients among kenaf germplasm resources were calculated by using analytic software NTSYSpc-2.10e, and phylogenetic tree was then established via UPGMA. [Result] Totally 169 bands were amplified using the 21 screened primers, averagely 8.05 bands were amplified from each primer. Of them, 141 bands were polymorphic, accounting for 83.4%. When genetic similarity coefficient 0.887 was used as criterion L1, these 44 shares of kenaf germplasm could be classified to be 32 shares of cultivars and 12 shares of wild type or half-wild type varieties. When genetic similarity coefficient 0.897 was used as criterion L2, these 32 shares of cultivars could be further grouped into four sub-clusters. The genetic diversities between cultivars and wild type or half-wild type varieties were between 0.46-0.91, showing huge hereditary difference; while that among 32 cultivars were between 0.85-0.97, suggesting that genetic relationships among cultivars are relatively close and their genetic similarities are rather narrow. [Conclusion] ISSR could well determine the genetic similarities among kenaf germplasm resources and provide valuable molecular information for selecting parents of hybrid cross, which can lay a good foundation for DNA mapping of kenaf germplasm resources.

Study on Genetic Diversity of Agronomic Traits and Genetic Relationships among Core Collections of Bitter Gourd

[Objective] This study aimed to investigate the genetic diversity of agronomic traits and genetic relationships among core collections of bitter gourd.[Method] Total 141 germplasms of bitter gourd were selected,and the genetic diversity of 13 agronomic traits was analyzed.In addition,total 46 core collections of bitter gourd were employed,and their genetic relationships were analyzed based on the phenotypic values and genotypic values of 5 agronomic traits,respectively.[Result] The genetic diversity analysis of agronomic traits showed that the genetic diversity indexes of the 4 qualitative traits of bitter gourd germplasms ranged from 0.46 to 1.34;the distribution of the 9 quantitative traits data was more dispersed with average coefficient of variation of 20.02%.The genetic relationship analysis showed that based on the phenotypic values and genotypic values of the 5 quantitative traits,the genetic distances among the 46 core collections of bitter gourd were different.Based on the genotypic values,the genetic distances among the 46 bitter gourd core collections ranged from 0.84 to 10.71.The 46 germplasms were divided into 17 groups with the rescaled distance of 8.5,which further classified the relationships among different germplasms.[Conclusion] This study will lay a solid foundation for the effective utilization of core collections and new variety breeding in bitter gourd.

Evaluation of Seed Oil Content in Cucumber Germplasm

With 35 different ecological types of cucumber germplasm resources as test materials, the soxhlet extraction was used to extract the crude fat from the cu- cumber seeds to analyze the seed oil content, with the aim to lay foundations for the application and new variety breeding related with cucumber seed oil. The results showed that there were significant differences in seed oil content between different cucumber varieties with the amplitude of variation rangeing from 31.03% to 40.59%, among which the seed oil content of south China cucumber was the highest of 40.59%, while the seed oil content of north China cucumber was the lowest of 31.03%. Four va.rieties with oil content of greater than 38.50% and four varieties with oil content of less than 32.01% werescreened out.

Resistance Evaluation of Capsicum spp. Germplasm to Meloidogyne incognita

[Objective] This study aimed to screen out hot pepper germplasms highly resistant to Meloidogyne incognita, thereby providing resistant resources for hot pep- per breeding. [Method] Comprehensive analysis combining cluster analysis and sub- ordinate function was conducted through determining related resistance indexes of 67 hot pepper germplasms 50 days after inoculated with M. incognita. [Result] The effects of M. incognita on related resistance indexes were significantly different am- ong the hot pepper germplasms. Egg index and gall index had abundant genetic variation with variation coefficients of 143.16% and 118.95%, respectively. Based on the gall indexes, cluster analysis of hot pepper germplasms was performed. The 67 hot pepper germplasms were divided into 4 groups （resistant, moderately resistant, susceptible and high susceptible）. The resistance intensity of the hot pepper germplasms were ranked according to the sum of subordinate function values of various resistance indexes. The total function values of Rela 2 and L506M were the largest （2.00）, indicating that these two germplasms were immune to M. incognita. The total function values of L287-2, L522-1M, L504M, L515-2, 13SM100-1, L512M, L292-1, L319, L316, L317, 13SM87-1 and Rela 5 were larger than 1.95, indicating that these germplasms were highly resistant to M. incognita. [Conclusion] This study could provide certain resistant resources for resistance breeding of hot pepper to M. incognita.

EST-SSR Marker-based Genetic Diversity Analysis of Rhododendron simsii Germplasm in Guifeng Mountain

Rhododendron simsii（Ericaeae：Rhododendron） has high ornamental value and ecological value.In this study,7 pairs of novel EST-SSR markers were developed from the genomic sequence of R.simsii,and they were used to investigate the genetic diversity of 32 natural R.simsii samples from Guifeng Mountain,Hubei Province.Results showed that a total of 31 polymorphic bands were amplified with allele number per locus of 4.43.Mean values of heterozygosity（Ho） and expected heterozygosity（He） were 0.679 58 and 0.723 14,respectively.This research will not only enrich the existing SSR database,but also lay a foundation for subsequent studies about molecular marker-assisted breeding,genetic diversity analysis,genetic structure analysis and phylogenetic analysis.

On the Identification and Evaluation of Salt Tolerance of Peanut Germplasm

For screening out salt tolerance germplasm and providing materials for ge- netic research of peanut, based on the indexes including relative germination poten- tial, relative germination rate, relative germination index and salt-injury rate, 128 peanut germplasms were selected for salt-tolerant identification and estimation under 2.5% NaCI. We found significant but various depressing levels of germination rate under salt stress among different germplasms, and only 5% of 128 germplasms were highly resistant to salinity. We also found that the relative germination index was a useful evaluation index for salt tolerance besides salt damage rate and rela- tive germination rate. After all, we comprehensively screened out 7 materials （JS011, JS024, JS125, JS491, JS523, JS524 and JS525） as salt tolerance germplasms for further research. Key

Fruit Anatomy of sect. Paracamellia with Reference to Their Significance of Germplasm Resources Exploitation

[Objective] To utilize the germplasm resources,new systematic evidence for the classification of sect.Paracamellia,genus Camellia is found in this article.[Method] Fruit anatomy of 10 species representing sect.Paracamellia was investigated.And the main component of sect.Paracamellia in the genus Camellia was studied by MVSP software.[Result] The exocarp was thin;mesocarp was broad,having a large number of stone cells;endocarp was lignified,and separated easily from seed.The principle components analysis suggested that the distance between species was far,showing far relation;the current study did not support the formation of Camellia grijsii（C.grijsii）and Camellia yuhsienensis（C.yuhsienensis）,Camellia shensiensis（C.shensiensis）should not be recognized as a variety of C.yuhsienensis.In addition,it also proved Ming＇s combining of Camellia confusa（C.confusa）and Camellia kissi（C.kissi）was unnatural.[Conclusion] The study provided theoretical basis for the development and application of sect.Paracamellia in the genus Camellia.