我国行政公益诉讼中的“过滤”机制

行政公益诉讼“过滤”机制,是检察机关从发现线索到提起诉讼这一过程中应当考虑的各种因素的有机集合体。目前的“过滤”机制存在着过于抽象、操作性不强的缺陷,对检察机关不具有太大的指导意义,这主要是因为制度建设初期经验不足以及未能充分吸收已有成果。从现有的立法、司法和学术理论出发,行政公益诉讼有三道“过滤”机制,第一道即提出检察建议的条件,第二道即检察建议自身,第三道即启动行政公益诉讼的条件。每一道“过滤”机制都有数个需要注意的重要问题,如要尽量扩大提起行政公益诉讼的范围,在针对行政不作为提起行政公益诉讼时应更为谨慎,解释“国家利益”和“社会公共利益”时不应过于严格,要特别注重检察建议的使用,等等。

Expression and Significance of PTEN in Bladder Transitional Cell Carcinoma

Objective： To explore the expression of anti-oncogene PTEN in bladder transitional cell carcinoma and its clinical significance. Methods： Using immunohistochemical S-P methods, the expression of PTEN gene was detected in 62 specimens of bladder cancer and 18 specimens of normal bladder tissue. Results： In the 62 bladder cancers, the positive rate of PTEN was 53.2% （33/62）. All 18 normal bladder tissues were positive for the PTEN expression. The expression of PTEN was negatively correlated to tumor grades （P〈0.05）. Conclusion： The reduced expression of PETN might play an important role in carcinogenesis and progression of bladder cancer. Detection of PTEN might be useful for judgement of tumor development and prognosis.

Viral and host causes of fatty liver in chronic hepatitis B

AIM: To investigate the viral and host causes of fatty liver in chronic hepatitis B patients and the role of fat deposits in liver damage.METHODS: A total of 164 patients (113 males and 51 females, average age 35±11.3 years, and range 10-62 years) with previously untreated chronic hepatitis B were included in the study. The patients were divided into two groups depending on the result of liver biopsy: group without steatosis (100 patients with ＜5% hepatosteatosis) and group with steatosis (64 patients with ＞5% hepatosteatosis). The groups were compared in terms of gender, body mass index (BMI), liver enzymes (ALT, AST, ALP, GGT), cholesterol, triglyceride, HBeAg, viral load, and histological findings. In the group with steatosis, the patients were subdivided depending on the degree of steatosis into mild group (45 patients with 5-24% steatosis), and severe group (19 patients with ＞25% steatosis). RESULTS: In the group of chronic hepatitis B with steatosis, the mean age, BMI, cholesterol, and triglyceride levels were significantly higher than those in the group without steatosis (P＜0.05). Steatosis was found in 53 (46.9%) of male patients and 11 (22%) of female patients (P＜0.05). No significant difference was found in the positivity of ALT, AST, ALP, GGT, HBeAg, viral load, histological activity index (HAI) and stage between the two groups (P＞0.05). In the group with severe steatosis, the BMI was significantly higher than that in the group with mild steatosis (P＜0.05). No significant difference was found in the other parameters between the groups (P＞0.05). CONCLUSION: Steatosis in chronic hepatitis B appears to be a result of metabolic factors of the host rather than the effect of viruses. Steatosis is unrelated to the HAI and degree of fibrosis, which are considered as the histological indicators of liver damage.

The Expression and Significance of KAl1 and Ki67 in Bladder Transitional Cell Carcinoma

OBJECTIVE To explore the expression and significance of the tumor metastatic suppressor gene the KAI1 and Ki67 antigen in bladder transitional cell carcinoma （BTCC）. METHODS Two-stepped immuno-histochemical staining was used to detect the expression of the KAI1 protein and Ki67 antigen. The reverse transcription polymerase chain reaction （RT-PCR） was used to detect the KAI1 mRNA in 54 BTCC specimens and 32 normal bladder counterparts. 13-actin was the internal control. RESULTS The KAI1 protein was mainly expressed on cell membranes at cell-to-cell borders, with uniform and continuous staining in normal bladder transitional epithelium. However, the number of positive-staining cells was greatly decreased in BTCC epithelium, and with an increase in the stage and Grade and appearance of lymph node metastasis the staining was non-uniform and discontinuous. The Ki67 antigen was expressed in the nucleus, and with an increase in the stage and Grade and appearance of lymph node metastasis, the Ki67 expression increased. The Ki67 antigen was negatively related to the expression of KAI1 （P〈0.01）.The expression level of KAI1 mRNA was much greater in normal bladder transitional epithelium compared to BTCC, moreover, with an increase in the Grade, infiltration depth and appearance of lymph node metastasis, the expression of KAI1 mRNA was reduced. CONCLUSION The expression of KAI1 protein may be used as a prognostic marker to indicate the degree of infiltration and metastasis. Measurement of KAI1 and Ki67 expression together may be helpful in evaluating the metastatic potential and prognosis of BTCC patients.

Research on fault detection method for heat pump air conditioning system under cold weather

Building energy consumption accounts for nearly 40% of global energy consumption, HVAC （Heating, Ventilating, and Air Conditioning） systems are the major building energy consumers, and as one type of HVAC systems, the heat pump air conditioning system, which is more energy-efficient compared to the traditional air conditioning system, is being more widely used to save energy. However, in northern China, extreme climatic conditions increase the cooling and heating load of the heat pump air conditioning system and accelerate the aging of the equipment, and the sensor may detect drifted parameters owing to climate change. This non-linear drifted parameter increases the false alarm rate of the fault detection and the need for unnecessary troubleshooting. In order to overcome the impact of the device aging and the drifted parameter, a Kalman filter and SPC （statistical process control） fault detection method are introduced in this paper. In this method, the model parameter and its standard variance can he estimated by Kalman filter based on the gray model and the real-time data of the air conditioning system. Further, by using SPC to construct the dynamic control limits, false alarm rate is reduced. And this paper mainly focuses on the cold machine failure in the component failure and its soft fault detection. This approach has been tested on a simulation model of the ＂Sino-German Energy Conservation Demonstration Center＂ building heat pump air-conditioning system in Shenyang, China, and the results show that the Kalman filter and SPC fault detection method is simple and highly efficient with a low false alarm rate, and it can deal with the difficulties caused by the extreme environment and the non-linear influence of the parameters, and what＇s more, it provides a good foundation for dynamic fault diagnosis and fault prediction analysis.

Multigene tracking of quasispecies in viral persistence and clearance of hepatitis C virus

AIM: To investigate the evaluation of hepatitis C virus (HCV) quasispecies in the envelope region and its relationship with the outcome of acute hepatitis C. METHODS: HCV quasispecies were characterized in specimens collected every 2-6 mo from a cohort of acutely HCV-infected subjects. We evaluated two individuals who spontaneously cleared viremia and three individuals with persistent viremia by cloning 33 1-kb amplicons that spanned E1 and the 5' half of E2, including hypervariable region 1 (HVR1). To assess the quasispecies complexity and to detect variants for sequencing, 33 cloned cDNAs representing each specimen were assessed bya combined method of analysis of a single-stranded conformational polymorphism and heteroduplex analysis. The rates of both synonymous and nonsynonymous substitutions for the E1, HVR1 and E2 regions outside HVR1 were analyzed.RESULTS: Serum samples collected from chronic phase of infection had higher quasispecies complexity than those collected from acute phase of infection in all individuals examined. The genetic diversity (genetic distance) within HVR1 was consistently higher than that in the complete E1 (0.0322±0.0068 vs-0.0020±0.0014, P＜0.05) and E2 regions outside HVR1 (0.0322±0.0068 vs0.0017±0.0011, P＜0.05) in individuals with persistent viremia, but did not change markedly over time in those with clearance of viremia. For individuals with persistent viremia, the rate of nonsynonymous substitutions within the HVR1 region (2.76×10-3±1.51×10-3) predominated and gradually increased, as compared with that in the E1 and E2 regions outside HVR1 (0.23×10-3±0.15×10-3, 0.50×10-3±0.10×10-3).By contrast, the rates of both nonsynonymous and synonymous substitutions for the E1 and E2 regions including HVR1 were consistently lower in individuals withclearance of viremia. CONCLUSION: HCV persistence is associated with a complexity quasispecies and positive selection of HVR1by the host immune system.

A new improvement of TCP based on adaptive filtering

On the basis of studying the standard TCP retransmission mechanism, this paper proposes a method to adopt the theory of adaptive filtering in the field for the estimation of round trip time （RTT）. Then this method is embedded in TCP for estimation of RTT, and defined as RTT-AF TCP. In the schemes with Only-TCP flow and with TCP and UDP flow fighting for the link, the experiment results were made and analyzed by using the standard TCP and the RTT-AF TCP. The experimental results show higher throughput and lower loss rate of packets by using RTT-AF TCP for data transmission, and achieve excellent effect.

纠纷格式化:立案过程中的纠纷转化研究

众多既有研究习惯性关注"纠纷及其解决",常常忽略纠纷本身的转化。本文基于对"立案"这一司法程序中纠纷转化环节的剖析,阐释生活世界的诸要素如何通过"纠纷格式化"进入法律世界。在纠纷转化为案件的过程中,系统化的法律逻辑与日常化的生活逻辑的交织互动持续发生,纠纷格式化的内涵包括对纠纷的定性、命名、形式化与文牍化等,并具体呈现为"内部过滤"、"外部包装"两大阶段,"过滤—包装"机制成为立案过程中纠纷转化的核心机制。这显示出主张程序正义的司法程序与约定俗成的惯例在互动中形成了一种互为制约、互为限度的紧张关系,同时也一定程度上实现了法律世界与生活世界的沟通融合。

Electrospinning with a spindle-knot structure for effective PM_(2.5) capture

Long-term air contamination and pollution challenges in particulate matter(PM) have raised fervent concerns for public health, e.g., the PM physical damage or the bacteria and virus carried by the PM. The desired air filter, seeking both high filtration efficiency and low pressure drop remains challenging. Here, we report a bio-inspired spindle-knot halloysite nanotube microsphere-incorporated nanofiber(HNM-NF) filter with the assembly and shape structures of the spider silk. The resulting HNM-NFs exhibit integrated properties of high surface energy, hydrophilicity and strong PM capture. The spindle-knot structures could shrink the outer pore size on two-dimensional(2 D) surface and construct the fluffy 3 D reticular architecture, facilitating high-efficiency air pollutant capture(>85.0%) while maintaining low resistance to airflow(~39 Pa). The spindle-knot construction method was applicable to various materials(i.e.,Al_(2)O_(3), ZnO and TiO_(2)) and volume production of the microsphere-incorporated NF cartridge. The diversified spindleknot construction will be valuable for adapting to meet different filtration requirements.