Granite is usually composed of quartz,biotite,feldspar,and cracks,and the variation characteristics of these components could reflect the deformation and failure process of rock well.Taking granite as an example,the v...Granite is usually composed of quartz,biotite,feldspar,and cracks,and the variation characteristics of these components could reflect the deformation and failure process of rock well.Taking granite as an example,the video camera was used to record the deformation and failure process of rock.The distribution of meso-components in video images was then identified.The meso-components of rock failure precursors were also discussed.Moreover,a modified LSTM(long short-term memory method)based on SSA(sparrow search algorithm)was proposed to estimate the change of meso-components of rock failure precursor.It shows that the initiation and expansion of cracks are mainly caused by feldspar and quartz fracture,and when the quartz and feldspar exit the stress framework,rock failure occurs;the second large increase of crack area and the second large decrease of quartz or feldspar area may be used as a precursor of rock failure;the precursor time of rock failure based on meso-scopic components is about 4 s earlier than that observed by the naked eye;the modified LSTM network has the strongest estimation ability for quartz area change,followed by feldspar and biotite,and has the worst estimation ability for cracks;when using the modified LSTM network to predict the precursors of rock instability and failure,quartz and feldspar could be given priority.The results presented herein may provide reference in the investigation of rock failure mechanism.展开更多
The classification of infrasound events has considerable importance in improving the capability to identify the types of natural disasters.The traditional infrasound classification mainly relies on machine learning al...The classification of infrasound events has considerable importance in improving the capability to identify the types of natural disasters.The traditional infrasound classification mainly relies on machine learning algorithms after artificial feature extraction.However,guaranteeing the effectiveness of the extracted features is difficult.The current trend focuses on using a convolution neural network to automatically extract features for classification.This method can be used to extract signal spatial features automatically through a convolution kernel;however,infrasound signals contain not only spatial information but also temporal information when used as a time series.These extracted temporal features are also crucial.If only a convolution neural network is used,then the time dependence of the infrasound sequence will be missed.Using long short-term memory networks can compensate for the missing time-series features but induces spatial feature information loss of the infrasound signal.A multiscale squeeze excitation–convolution neural network–bidirectional long short-term memory network infrasound event classification fusion model is proposed in this study to address these problems.This model automatically extracted temporal and spatial features,adaptively selected features,and also realized the fusion of the two types of features.Experimental results showed that the classification accuracy of the model was more than 98%,thus verifying the effectiveness and superiority of the proposed model.展开更多
BACKGROUND:Adeno-associated virus(AAV)gene therapy has been proven to be reliable and safe for the treatment of osteoarthritis in recent years.However,given the complexity of osteoarthritis pathogenesis,single gene ma...BACKGROUND:Adeno-associated virus(AAV)gene therapy has been proven to be reliable and safe for the treatment of osteoarthritis in recent years.However,given the complexity of osteoarthritis pathogenesis,single gene manipulation for the treatment of osteoarthritis may not produce satisfactory results.Previous studies have shown that nuclear factorκB could promote the inflammatory pathway in osteoarthritic chondrocytes,and bone morphogenetic protein 4(BMP4)could promote cartilage regeneration.OBJECTIVE:To test whether combined application of AAV-p65shRNA and AAV-BMP4 will yield the synergistic effect on chondrocytes regeneration and osteoarthritis treatment.METHODS:Viral particles containing AAV-p65-shRNA and AAV-BMP4 were prepared.Their efficacy in inhibiting inflammation in chondrocytes and promoting chondrogenesis was assessed in vitro and in vivo by transfecting AAV-p65-shRNA or AAV-BMP4 into cells.The experiments were divided into five groups:PBS group;osteoarthritis group;AAV-BMP4 group;AAV-p65shRNA group;and BMP4-p65shRNA 1:1 group.Samples were collected at 4,12,and 24 weeks postoperatively.Tissue staining,including safranin O and Alcian blue,was applied after collecting articular tissue.Then,the optimal ratio between the two types of transfected viral particles was further investigated to improve the chondrogenic potential of mixed cells in vivo.RESULTS AND CONCLUSION:The combined application of AAV-p65shRNA and AAV-BMP4 together showed a synergistic effect on cartilage regeneration and osteoarthritis treatment.Mixed cells transfected with AAV-p65shRNA and AAV-BMP4 at a 1:1 ratio produced the most extracellular matrix synthesis(P<0.05).In vivo results also revealed that the combination of the two viruses had the highest regenerative potential for osteoarthritic cartilage(P<0.05).In the present study,we also discovered that the combined therapy had the maximum effect when the two viruses were administered in equal proportions.Decreasing either p65shRNA or BMP4 transfected cells resulted in less collagen II synthesis.This implies that inhibiting inflammation by p65shRNA and promoting regeneration by BMP4 are equally important for osteoarthritis treatment.These findings provide a new strategy for the treatment of early osteoarthritis by simultaneously inhibiting cartilage inflammation and promoting cartilage repair.展开更多
Five species of scorpionflies in the genus Panorpa Linn. are described and illustrated from the Changbai Mountains in Jilin Province, northeast China. Among them, four species are described as new to science, i.e...Five species of scorpionflies in the genus Panorpa Linn. are described and illustrated from the Changbai Mountains in Jilin Province, northeast China. Among them, four species are described as new to science, i.e. Panorpa macrostyla sp.n., Panorpa brevicaudata sp.n., Panorpa choui sp.n., and Panorpa acutipennis sp.n. The fifth species, Panorpa liui Hua, is newly recorded from the province.展开更多
On the basis of information theory and statistical methods, we use mutual information, n- tuple entropy and conditional entropy, combined with biological characteristics, to analyze the long range correlation and shor...On the basis of information theory and statistical methods, we use mutual information, n- tuple entropy and conditional entropy, combined with biological characteristics, to analyze the long range correlation and short range correlation in human Y chromosome palindromes. The magnitude distribution of the long range correlation which can be reflected by the mutual information is PS〉PSa〉PSb （P5a and P5b are the sequences that replace solely Alu repeats and all interspersed repeats with random uneorrelated sequences in human Y chromosome palindrome 5, respectively）; and the magnitude distribution of the short range correlation which can be reflected by the n-tuple entropy and the conditional entropy is PS〉P5a〉PSb〉random uncorrelated sequence. In other words, when the Alu repeats and all interspersed repeats replace with random uneorrelated sequence, the long range and short range correlation decrease gradually. However, the random nncorrelated sequence has no correlation. This research indicates that more repeat sequences result in stronger correlation between bases in human Y chromosome. The analyses may be helpful to understand the special structures of human Y chromosome palindromes profoundly.展开更多
The influence of long regular waves on wind waves are examined in the laboratory tank. The wave spectra of wind waves are compared when there is and there is not long waves. Besides the widely addressed suppression of...The influence of long regular waves on wind waves are examined in the laboratory tank. The wave spectra of wind waves are compared when there is and there is not long waves. Besides the widely addressed suppression of wind waves by long waves, it is also found that, the presence of long regular wave induces low frequency shift of wind waves when long wave slope is small and also its frequencyf is quite apart from wind wave crest frequencies fp. The effect of long wave modulation on wind wave spectra is estimated according to Longuet-Higgins ＆ Stewart （1960） （abbreviated as LS60 afterwards）, which is found to be prominent at the large ratio of fp/f l. It＇s also found that, when the limitation of wave breaking on wind wave steepness is taken account of, the LS60 theory can explain the low frequency shift satisfactorily. The work suggests that, at small long wave slope and large ratio of fp/fl, the LS60 modulation mechanism together with the enhanced wave breaking may dominate the influence of long waves on wind waves.展开更多
An improved Gaussian mixture model （GMM）- based clustering method is proposed for the difficult case where the true distribution of data is against the assumed GMM. First, an improved model selection criterion, the ...An improved Gaussian mixture model （GMM）- based clustering method is proposed for the difficult case where the true distribution of data is against the assumed GMM. First, an improved model selection criterion, the completed likelihood minimum message length criterion, is derived. It can measure both the goodness-of-fit of the candidate GMM to the data and the goodness-of-partition of the data. Secondly, by utilizing the proposed criterion as the clustering objective function, an improved expectation- maximization （EM） algorithm is developed, which can avoid poor local optimal solutions compared to the standard EM algorithm for estimating the model parameters. The experimental results demonstrate that the proposed method can rectify the over-fitting tendency of representative GMM-based clustering approaches and can robustly provide more accurate clustering results.展开更多
Dolichothyreus Usinger ＆ Matsuda, 1959 and D. stigmatus Usinger ＆ Matsuda are recorded from China for the first time. The male of D. stigmatus is redescribed and the diagnostic morphological features of species are...Dolichothyreus Usinger ＆ Matsuda, 1959 and D. stigmatus Usinger ＆ Matsuda are recorded from China for the first time. The male of D. stigmatus is redescribed and the diagnostic morphological features of species are illustrated.展开更多
In order to study the influence of microstructural texture on the growth of short fatigue cracks in metals, the nonequilibrium statistical theory of fatigue fracture correlating a microscopic mechanism with the macros...In order to study the influence of microstructural texture on the growth of short fatigue cracks in metals, the nonequilibrium statistical theory of fatigue fracture correlating a microscopic mechanism with the macroscopic properties is modified to take into consideration the microstructural features of a material, thereby allowing a rationalisation of the experimental data of short fatigue crack growth and long fatigue crack growth. The nonequilibrium statistical theory thus developed relates the growth of cracks with a dislocation mechanism to simulate short fatigue crack growth with the long fatigue crack growth behaviour and predicts the fatigue crack growth rates throughout the fatigue lifetime. The results is finally compared with that of other fatigue theories.展开更多
基金Project(41472254)supported by the National Natural Science Foundation of China。
文摘Granite is usually composed of quartz,biotite,feldspar,and cracks,and the variation characteristics of these components could reflect the deformation and failure process of rock well.Taking granite as an example,the video camera was used to record the deformation and failure process of rock.The distribution of meso-components in video images was then identified.The meso-components of rock failure precursors were also discussed.Moreover,a modified LSTM(long short-term memory method)based on SSA(sparrow search algorithm)was proposed to estimate the change of meso-components of rock failure precursor.It shows that the initiation and expansion of cracks are mainly caused by feldspar and quartz fracture,and when the quartz and feldspar exit the stress framework,rock failure occurs;the second large increase of crack area and the second large decrease of quartz or feldspar area may be used as a precursor of rock failure;the precursor time of rock failure based on meso-scopic components is about 4 s earlier than that observed by the naked eye;the modified LSTM network has the strongest estimation ability for quartz area change,followed by feldspar and biotite,and has the worst estimation ability for cracks;when using the modified LSTM network to predict the precursors of rock instability and failure,quartz and feldspar could be given priority.The results presented herein may provide reference in the investigation of rock failure mechanism.
基金supported by the Shaanxi Province Natural Science Basic Research Plan Project(2023-JC-YB-244).
文摘The classification of infrasound events has considerable importance in improving the capability to identify the types of natural disasters.The traditional infrasound classification mainly relies on machine learning algorithms after artificial feature extraction.However,guaranteeing the effectiveness of the extracted features is difficult.The current trend focuses on using a convolution neural network to automatically extract features for classification.This method can be used to extract signal spatial features automatically through a convolution kernel;however,infrasound signals contain not only spatial information but also temporal information when used as a time series.These extracted temporal features are also crucial.If only a convolution neural network is used,then the time dependence of the infrasound sequence will be missed.Using long short-term memory networks can compensate for the missing time-series features but induces spatial feature information loss of the infrasound signal.A multiscale squeeze excitation–convolution neural network–bidirectional long short-term memory network infrasound event classification fusion model is proposed in this study to address these problems.This model automatically extracted temporal and spatial features,adaptively selected features,and also realized the fusion of the two types of features.Experimental results showed that the classification accuracy of the model was more than 98%,thus verifying the effectiveness and superiority of the proposed model.
文摘BACKGROUND:Adeno-associated virus(AAV)gene therapy has been proven to be reliable and safe for the treatment of osteoarthritis in recent years.However,given the complexity of osteoarthritis pathogenesis,single gene manipulation for the treatment of osteoarthritis may not produce satisfactory results.Previous studies have shown that nuclear factorκB could promote the inflammatory pathway in osteoarthritic chondrocytes,and bone morphogenetic protein 4(BMP4)could promote cartilage regeneration.OBJECTIVE:To test whether combined application of AAV-p65shRNA and AAV-BMP4 will yield the synergistic effect on chondrocytes regeneration and osteoarthritis treatment.METHODS:Viral particles containing AAV-p65-shRNA and AAV-BMP4 were prepared.Their efficacy in inhibiting inflammation in chondrocytes and promoting chondrogenesis was assessed in vitro and in vivo by transfecting AAV-p65-shRNA or AAV-BMP4 into cells.The experiments were divided into five groups:PBS group;osteoarthritis group;AAV-BMP4 group;AAV-p65shRNA group;and BMP4-p65shRNA 1:1 group.Samples were collected at 4,12,and 24 weeks postoperatively.Tissue staining,including safranin O and Alcian blue,was applied after collecting articular tissue.Then,the optimal ratio between the two types of transfected viral particles was further investigated to improve the chondrogenic potential of mixed cells in vivo.RESULTS AND CONCLUSION:The combined application of AAV-p65shRNA and AAV-BMP4 together showed a synergistic effect on cartilage regeneration and osteoarthritis treatment.Mixed cells transfected with AAV-p65shRNA and AAV-BMP4 at a 1:1 ratio produced the most extracellular matrix synthesis(P<0.05).In vivo results also revealed that the combination of the two viruses had the highest regenerative potential for osteoarthritic cartilage(P<0.05).In the present study,we also discovered that the combined therapy had the maximum effect when the two viruses were administered in equal proportions.Decreasing either p65shRNA or BMP4 transfected cells resulted in less collagen II synthesis.This implies that inhibiting inflammation by p65shRNA and promoting regeneration by BMP4 are equally important for osteoarthritis treatment.These findings provide a new strategy for the treatment of early osteoarthritis by simultaneously inhibiting cartilage inflammation and promoting cartilage repair.
文摘Five species of scorpionflies in the genus Panorpa Linn. are described and illustrated from the Changbai Mountains in Jilin Province, northeast China. Among them, four species are described as new to science, i.e. Panorpa macrostyla sp.n., Panorpa brevicaudata sp.n., Panorpa choui sp.n., and Panorpa acutipennis sp.n. The fifth species, Panorpa liui Hua, is newly recorded from the province.
基金This work was supported by the National Natu- ral Science Foundation of China （No.20173023 and No.90203012） and the Specialized Research Fund for the Doctoral Program of Higher Education of China （No.20020730006）.
文摘On the basis of information theory and statistical methods, we use mutual information, n- tuple entropy and conditional entropy, combined with biological characteristics, to analyze the long range correlation and short range correlation in human Y chromosome palindromes. The magnitude distribution of the long range correlation which can be reflected by the mutual information is PS〉PSa〉PSb （P5a and P5b are the sequences that replace solely Alu repeats and all interspersed repeats with random uneorrelated sequences in human Y chromosome palindrome 5, respectively）; and the magnitude distribution of the short range correlation which can be reflected by the n-tuple entropy and the conditional entropy is PS〉P5a〉PSb〉random uncorrelated sequence. In other words, when the Alu repeats and all interspersed repeats replace with random uneorrelated sequence, the long range and short range correlation decrease gradually. However, the random nncorrelated sequence has no correlation. This research indicates that more repeat sequences result in stronger correlation between bases in human Y chromosome. The analyses may be helpful to understand the special structures of human Y chromosome palindromes profoundly.
文摘The influence of long regular waves on wind waves are examined in the laboratory tank. The wave spectra of wind waves are compared when there is and there is not long waves. Besides the widely addressed suppression of wind waves by long waves, it is also found that, the presence of long regular wave induces low frequency shift of wind waves when long wave slope is small and also its frequencyf is quite apart from wind wave crest frequencies fp. The effect of long wave modulation on wind wave spectra is estimated according to Longuet-Higgins ＆ Stewart （1960） （abbreviated as LS60 afterwards）, which is found to be prominent at the large ratio of fp/f l. It＇s also found that, when the limitation of wave breaking on wind wave steepness is taken account of, the LS60 theory can explain the low frequency shift satisfactorily. The work suggests that, at small long wave slope and large ratio of fp/fl, the LS60 modulation mechanism together with the enhanced wave breaking may dominate the influence of long waves on wind waves.
基金The National Natural Science Foundation of China(No.61105048,60972165)the Doctoral Fund of Ministry of Education of China(No.20110092120034)+2 种基金the Natural Science Foundation of Jiangsu Province(No.BK2010240)the Technology Foundation for Selected Overseas Chinese Scholar,Ministry of Human Resources and Social Security of China(No.6722000008)the Open Fund of Jiangsu Province Key Laboratory for Remote Measuring and Control(No.YCCK201005)
文摘An improved Gaussian mixture model （GMM）- based clustering method is proposed for the difficult case where the true distribution of data is against the assumed GMM. First, an improved model selection criterion, the completed likelihood minimum message length criterion, is derived. It can measure both the goodness-of-fit of the candidate GMM to the data and the goodness-of-partition of the data. Secondly, by utilizing the proposed criterion as the clustering objective function, an improved expectation- maximization （EM） algorithm is developed, which can avoid poor local optimal solutions compared to the standard EM algorithm for estimating the model parameters. The experimental results demonstrate that the proposed method can rectify the over-fitting tendency of representative GMM-based clustering approaches and can robustly provide more accurate clustering results.
基金grants from Natural Science Foundation of Beijing (6083023)the National Natural Science and Technology Basic Platform Projects (2005DKA21105,2005DKA21402)Hainan Biological Resources Survey Project (2006FY110500)
文摘Dolichothyreus Usinger ＆ Matsuda, 1959 and D. stigmatus Usinger ＆ Matsuda are recorded from China for the first time. The male of D. stigmatus is redescribed and the diagnostic morphological features of species are illustrated.
文摘In order to study the influence of microstructural texture on the growth of short fatigue cracks in metals, the nonequilibrium statistical theory of fatigue fracture correlating a microscopic mechanism with the macroscopic properties is modified to take into consideration the microstructural features of a material, thereby allowing a rationalisation of the experimental data of short fatigue crack growth and long fatigue crack growth. The nonequilibrium statistical theory thus developed relates the growth of cracks with a dislocation mechanism to simulate short fatigue crack growth with the long fatigue crack growth behaviour and predicts the fatigue crack growth rates throughout the fatigue lifetime. The results is finally compared with that of other fatigue theories.
