During the past few decades, there has been a shift in the focus of language research, from how to teach to how to learn. And among so many learning strategies, metacognitive strategies have gradually aroused research...During the past few decades, there has been a shift in the focus of language research, from how to teach to how to learn. And among so many learning strategies, metacognitive strategies have gradually aroused researchers' attention. However, in China. there is a lack of studies on the application of metacognitive strategies in English reading, especially those conducted towards high school students. The present thesis introduces metacognitive strategies and its application in English reading of senior high school, which is based on the analysis of the current status of reading teaching and learning.展开更多
This experimental study investigated how text difficulty and different working memory capacity(WMC)affected Chinese EFL learners’reading comprehension and their tendency to engage in task-unrelated thoughts,that is,m...This experimental study investigated how text difficulty and different working memory capacity(WMC)affected Chinese EFL learners’reading comprehension and their tendency to engage in task-unrelated thoughts,that is,mind wandering(MW),in the course of reading.Sixty first-year university non-English majors participated in the study.A two-factor mixed experimental design of 2(text difficulty:difficult and simple)×2(WMC:high/large and low/small)was employed.Results revealed that 1)the main and interaction effects of WMC and text difficulty on voluntary MW were significant,whereas those on involuntary MW were not;2)while reading the easy texts,the involuntary MW of high-WMC individuals was less frequent than that of low-WMC ones,whereas while reading the difficult ones,the direct relationship between WMC and involuntary MW was not found;and that 3)high-WMC individuals had a lower overall rate of MW and better reading performance than low-WMC individuals did,but with increasing text difficulty,their rates of overall MW and voluntary MW were getting higher and higher,and the reading performance was getting lower and lower.These results lend support to WM theory and have pedagogical implications for the instruction of L2 reading.展开更多
A quadrature mixer with a shared transconductor stage is analyzed,including voltage conversion gain, linearity, noise figure, and image rejection. The analysis indicates it has better performance than a conventional G...A quadrature mixer with a shared transconductor stage is analyzed,including voltage conversion gain, linearity, noise figure, and image rejection. The analysis indicates it has better performance than a conventional Gilbert mixer pair in commutating mode. A quadrature down-conversion mixer based on this topology is designed and optimized for an ultra high frequency RFID reader. Operating in the 915MHz ISM band, the presented quadrature mixer measures a conversion gain of 12.5dB,an IIP3 of 10dBm, an IIP2 of 58dBm, and an SSB noise figure of 17.6riB. The chip was fabricated in a 0. 18μm 1P6M RF CMOS process and consumes only 3mA of current from a 1.8V power supply.展开更多
This paper makes an analysis of the cognitive nature of metaphor, expounds the view that metaphor is the necessity of cognitive process of vocabulary and reading teaching, especially for the English major. By employin...This paper makes an analysis of the cognitive nature of metaphor, expounds the view that metaphor is the necessity of cognitive process of vocabulary and reading teaching, especially for the English major. By employing some typical examples from the English-major textbooks, it advances how to train and raise specific teaching ways by utilizing metaphorical thinking.展开更多
Reading and writing are input and output respectively. The relation between reading and writing is closely related. Reading is the preparation for good writing. And writing theory or law can provide readers some clues...Reading and writing are input and output respectively. The relation between reading and writing is closely related. Reading is the preparation for good writing. And writing theory or law can provide readers some clues for better understanding. The relation between reading and writing can help our English teaching in and out class. Also the way of thinking influences our reading and writing.展开更多
Transitivity system is one of the frequently used analytical tools in critical linguistics. It has often been considered as a useful analytical model, and employed both in stylistics and critical linguistics. This pap...Transitivity system is one of the frequently used analytical tools in critical linguistics. It has often been considered as a useful analytical model, and employed both in stylistics and critical linguistics. This paper briefly introduces the transitivity model and its applications within critical linguistics based on the analysis of an article of media discourse, and aims to raise readers' critical language awareness to improve their critical reading ability.展开更多
The human beings can express the outside world and their mental feelings by languages, they know the materials' nature by comparing with their similarities. The languages are not only tools in understanding,communica...The human beings can express the outside world and their mental feelings by languages, they know the materials' nature by comparing with their similarities. The languages are not only tools in understanding,communication and thinking, but also one element of the cultures. Thinking is a kind of abstract skill, and the language is the vehicle of thinking, through which people can understand the human beings' thinking methods and cultural characters. The languages are decided by the culture. Reading comprehension is the readers' own behavior, during which the schema plays a key role and readers must combine the new information with their former cultural knowledge, rebuilding the new information so as to understand the deep meanings of the articles.展开更多
Language teaching is a highly complex phenomenon so that it is closely related with philosophy of education, theoretical linguistics and cognitive science. The language system is constructed by adopting a series of co...Language teaching is a highly complex phenomenon so that it is closely related with philosophy of education, theoretical linguistics and cognitive science. The language system is constructed by adopting a series of cognitive strategies within human beings' experiences. On the basis of outlook of experience, reading and comprehension can be regarded as a process of prediction, perception, reasoning and retrieving, in which the reader is an experiencing individual. Thus, experience is a clue in the reading process.展开更多
Based on the interactive theory of reading, this study mainly explored the etlects ot topic familiarity and second language proficiency on IVA (Incidental Vocabulary Acquisition) of second language through reading. ...Based on the interactive theory of reading, this study mainly explored the etlects ot topic familiarity and second language proficiency on IVA (Incidental Vocabulary Acquisition) of second language through reading. By using two different measures (translation production and selection), this study found: (1) Vocabulary can be acquired incidentally in reading passages; (2) There are no significant interactive effects of topic familiarity and second language proficiency on vocabulary acquisition, but the two independent variables of topic familiarity and second language proficiency exerted their positively significant effect on incidental vocabulary acquisition, and (3) As for the two vocabulary measures, learners can acquire more words in translation selection than in translation production.展开更多
Generation of induced pluripotent stem (iPS) cells from somatic cells has been achieved successfully by simultaneous viral transduction of defined reprogramming transcription factors (TFs). However, the process re...Generation of induced pluripotent stem (iPS) cells from somatic cells has been achieved successfully by simultaneous viral transduction of defined reprogramming transcription factors (TFs). However, the process requires multiple viral vectors for gene delivery. As a result, generated iPS cells harbor numerous viral integration sites in their genomes. This can increase the probability of gene mutagenesis and genomic instability, and present significant barriers to both research and clinical application studies of iPS cells. In this paper, we present a simple lentivirus reprogramming system in which defined factors are fused in-frame into a single open reading frame (ORF) via self-cleaving 2A sequences. A GFP marker is placed downstream of the transgene to enable tracking of transgene expression. We demonstrate that this polycistronic expression system efficiently generates iPS cells. The generated iPS cells have normal karyotypes and are similar to mouse embryonic stem cells in morphology and gene expression. Moreover, they can differentiate into cell types of the three embryonic germ layers in both in vitro and in vivo assays. Remarkably, most of these iPS cells only harbor a single copy of viral vector. This system provides a valuable tool for generation of iPS cells, and our data suggest that the balance of expression of transduced reprogramming TFs in each cell is essential for the reprogramming process. More importantly, when delivered by non-integrating gene-delivery systems, this re-engineered single ORF will facilitate efficient generation of human iPS cells free of genetic modifications.展开更多
The fifth generation (5G) wireless communication is currently a hot research topic and wireless communication systems on high speed railways (HSR) are important applications of 5G technologies. Existing stud- ies ...The fifth generation (5G) wireless communication is currently a hot research topic and wireless communication systems on high speed railways (HSR) are important applications of 5G technologies. Existing stud- ies about 5G wireless systems on high speed railways (HSR) often utilize ideal channel parameters and are usually based on simple scenarios. In this paper, we evaluate the down- link throughput of 5G HSR communication systems on three typical scenarios including urban, cutting and viaduct with three different channel estimators. The channel parameters of each scenario are generated with tapped delay line (TDL) models through ray-tracing sim- ulations, which can be considered as a good match to practical situations. The channel estimators including least square (LS), linear minimum mean square error (LMMSE), and our proposed historical information based ba- sis expansion model (HiBEM). We analyze the performance of the HiBEM estimator in terms of mean square error (MSE) and evaluate the system throughputs with different channel estimates over each scenario. Simulation results are then provided to corroborate our proposed studies. It is shown that our HiBEM estimator outperforms other estimators and that the sys-tem throughput can reach the highest point in the viaduct scenario.展开更多
An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. c...An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene.展开更多
A substantial part of the human genome is derived from transposable elements;remnants of ancient retroviral infections.Conservative estimates set the percentage of human endogenous retroviruses(HERVs) in the genome at...A substantial part of the human genome is derived from transposable elements;remnants of ancient retroviral infections.Conservative estimates set the percentage of human endogenous retroviruses(HERVs) in the genome at 8%.For the most part,the interplay between mutations,epigenetic mechanisms and posttranscriptional regulations silence HERVs in somatic cells.We first highlight mechanisms by which activation of members of several HERV families may be associated with tumor development before discussing the arising chances for both diagnosis and therapy.It has been shown that at least in some cases,tumor cells expressing HERV open reading frames(ORFs) thus gain tumor-promoting functions.However,since these proteins are not expressed in healthy tissues,they become prime target structures.Of potential pharmacological interest are the prevention of HERV transposition,the inhibition of HERV-encoded protein expression and the interference with these proteins' activities.Evidence from recent studies unequivocally proves that HERV ORFs represent a very interesting source of novel tumor-specific antigens with even the potential to surpass entity boundaries.The development of new tumor(immune-) therapies is a very active field and true tumor-specific targets are of outstanding interest since they minimize the risk of autoimmunity and could reduce side effects.Finally,we postulate on main future research streams in order to stimulate discussion on this hot topic.展开更多
Objective To investigate the variability of human cytomegalovirus (HCMV) UL138 open reading frame (ORF) in clinical strains. Methods HCMV UL138 ORF was amplified by polymerase chain reaction (PCR) and PCR amplif...Objective To investigate the variability of human cytomegalovirus (HCMV) UL138 open reading frame (ORF) in clinical strains. Methods HCMV UL138 ORF was amplified by polymerase chain reaction (PCR) and PCR amplification products were sequenced directly, and the data were analyzed in 19 clinical strains. Results LIL138 ORF in all 30 clinical strains was amplified successfully. Compared with that of Toledo strain, the nucleotide and amino acid sequence identifies of LIL138 ORF in all strains were 97.41% to 99.41% and 98.24% to 99.42%, respectively. All of the nucleofide mutations were substitutions. The spatial structure and post-translational modification sites of HL138 encoded proteins were conserved. The result of phylogenetic tree showed that HCMV HL138 sequence variations were not definitely related with different clinical symptoms. Conclusion HCMV UL138 ORF in clinical strains is high conservation, which might be helpful for UL138 encoded protein to play a role in latent infection of HCMV.展开更多
文摘During the past few decades, there has been a shift in the focus of language research, from how to teach to how to learn. And among so many learning strategies, metacognitive strategies have gradually aroused researchers' attention. However, in China. there is a lack of studies on the application of metacognitive strategies in English reading, especially those conducted towards high school students. The present thesis introduces metacognitive strategies and its application in English reading of senior high school, which is based on the analysis of the current status of reading teaching and learning.
文摘This experimental study investigated how text difficulty and different working memory capacity(WMC)affected Chinese EFL learners’reading comprehension and their tendency to engage in task-unrelated thoughts,that is,mind wandering(MW),in the course of reading.Sixty first-year university non-English majors participated in the study.A two-factor mixed experimental design of 2(text difficulty:difficult and simple)×2(WMC:high/large and low/small)was employed.Results revealed that 1)the main and interaction effects of WMC and text difficulty on voluntary MW were significant,whereas those on involuntary MW were not;2)while reading the easy texts,the involuntary MW of high-WMC individuals was less frequent than that of low-WMC ones,whereas while reading the difficult ones,the direct relationship between WMC and involuntary MW was not found;and that 3)high-WMC individuals had a lower overall rate of MW and better reading performance than low-WMC individuals did,but with increasing text difficulty,their rates of overall MW and voluntary MW were getting higher and higher,and the reading performance was getting lower and lower.These results lend support to WM theory and have pedagogical implications for the instruction of L2 reading.
文摘A quadrature mixer with a shared transconductor stage is analyzed,including voltage conversion gain, linearity, noise figure, and image rejection. The analysis indicates it has better performance than a conventional Gilbert mixer pair in commutating mode. A quadrature down-conversion mixer based on this topology is designed and optimized for an ultra high frequency RFID reader. Operating in the 915MHz ISM band, the presented quadrature mixer measures a conversion gain of 12.5dB,an IIP3 of 10dBm, an IIP2 of 58dBm, and an SSB noise figure of 17.6riB. The chip was fabricated in a 0. 18μm 1P6M RF CMOS process and consumes only 3mA of current from a 1.8V power supply.
文摘This paper makes an analysis of the cognitive nature of metaphor, expounds the view that metaphor is the necessity of cognitive process of vocabulary and reading teaching, especially for the English major. By employing some typical examples from the English-major textbooks, it advances how to train and raise specific teaching ways by utilizing metaphorical thinking.
文摘Reading and writing are input and output respectively. The relation between reading and writing is closely related. Reading is the preparation for good writing. And writing theory or law can provide readers some clues for better understanding. The relation between reading and writing can help our English teaching in and out class. Also the way of thinking influences our reading and writing.
文摘Transitivity system is one of the frequently used analytical tools in critical linguistics. It has often been considered as a useful analytical model, and employed both in stylistics and critical linguistics. This paper briefly introduces the transitivity model and its applications within critical linguistics based on the analysis of an article of media discourse, and aims to raise readers' critical language awareness to improve their critical reading ability.
文摘The human beings can express the outside world and their mental feelings by languages, they know the materials' nature by comparing with their similarities. The languages are not only tools in understanding,communication and thinking, but also one element of the cultures. Thinking is a kind of abstract skill, and the language is the vehicle of thinking, through which people can understand the human beings' thinking methods and cultural characters. The languages are decided by the culture. Reading comprehension is the readers' own behavior, during which the schema plays a key role and readers must combine the new information with their former cultural knowledge, rebuilding the new information so as to understand the deep meanings of the articles.
文摘Language teaching is a highly complex phenomenon so that it is closely related with philosophy of education, theoretical linguistics and cognitive science. The language system is constructed by adopting a series of cognitive strategies within human beings' experiences. On the basis of outlook of experience, reading and comprehension can be regarded as a process of prediction, perception, reasoning and retrieving, in which the reader is an experiencing individual. Thus, experience is a clue in the reading process.
文摘Based on the interactive theory of reading, this study mainly explored the etlects ot topic familiarity and second language proficiency on IVA (Incidental Vocabulary Acquisition) of second language through reading. By using two different measures (translation production and selection), this study found: (1) Vocabulary can be acquired incidentally in reading passages; (2) There are no significant interactive effects of topic familiarity and second language proficiency on vocabulary acquisition, but the two independent variables of topic familiarity and second language proficiency exerted their positively significant effect on incidental vocabulary acquisition, and (3) As for the two vocabulary measures, learners can acquire more words in translation selection than in translation production.
文摘Generation of induced pluripotent stem (iPS) cells from somatic cells has been achieved successfully by simultaneous viral transduction of defined reprogramming transcription factors (TFs). However, the process requires multiple viral vectors for gene delivery. As a result, generated iPS cells harbor numerous viral integration sites in their genomes. This can increase the probability of gene mutagenesis and genomic instability, and present significant barriers to both research and clinical application studies of iPS cells. In this paper, we present a simple lentivirus reprogramming system in which defined factors are fused in-frame into a single open reading frame (ORF) via self-cleaving 2A sequences. A GFP marker is placed downstream of the transgene to enable tracking of transgene expression. We demonstrate that this polycistronic expression system efficiently generates iPS cells. The generated iPS cells have normal karyotypes and are similar to mouse embryonic stem cells in morphology and gene expression. Moreover, they can differentiate into cell types of the three embryonic germ layers in both in vitro and in vivo assays. Remarkably, most of these iPS cells only harbor a single copy of viral vector. This system provides a valuable tool for generation of iPS cells, and our data suggest that the balance of expression of transduced reprogramming TFs in each cell is essential for the reprogramming process. More importantly, when delivered by non-integrating gene-delivery systems, this re-engineered single ORF will facilitate efficient generation of human iPS cells free of genetic modifications.
基金supported by the National Natural Science Foundation of China(Grant Nos.61522109,61671253,61571037and 91738201)the Fundamental Research Funds for the Central Universities(No.2016JBZ006)+1 种基金the Natural Science Foundation of Jiangsu Province(Grant Nos.BK20150040and BK20171446)the Key Project of Natural Science Research of Higher Education Institutions of Jiangsu Province(No.15KJA510003)
文摘The fifth generation (5G) wireless communication is currently a hot research topic and wireless communication systems on high speed railways (HSR) are important applications of 5G technologies. Existing stud- ies about 5G wireless systems on high speed railways (HSR) often utilize ideal channel parameters and are usually based on simple scenarios. In this paper, we evaluate the down- link throughput of 5G HSR communication systems on three typical scenarios including urban, cutting and viaduct with three different channel estimators. The channel parameters of each scenario are generated with tapped delay line (TDL) models through ray-tracing sim- ulations, which can be considered as a good match to practical situations. The channel estimators including least square (LS), linear minimum mean square error (LMMSE), and our proposed historical information based ba- sis expansion model (HiBEM). We analyze the performance of the HiBEM estimator in terms of mean square error (MSE) and evaluate the system throughputs with different channel estimates over each scenario. Simulation results are then provided to corroborate our proposed studies. It is shown that our HiBEM estimator outperforms other estimators and that the sys-tem throughput can reach the highest point in the viaduct scenario.
基金Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)
文摘An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene.
基金Supported by Grants from the State Mecklenburg-Vorpommern and from Deutsche Krebshilfe,No.108446
文摘A substantial part of the human genome is derived from transposable elements;remnants of ancient retroviral infections.Conservative estimates set the percentage of human endogenous retroviruses(HERVs) in the genome at 8%.For the most part,the interplay between mutations,epigenetic mechanisms and posttranscriptional regulations silence HERVs in somatic cells.We first highlight mechanisms by which activation of members of several HERV families may be associated with tumor development before discussing the arising chances for both diagnosis and therapy.It has been shown that at least in some cases,tumor cells expressing HERV open reading frames(ORFs) thus gain tumor-promoting functions.However,since these proteins are not expressed in healthy tissues,they become prime target structures.Of potential pharmacological interest are the prevention of HERV transposition,the inhibition of HERV-encoded protein expression and the interference with these proteins' activities.Evidence from recent studies unequivocally proves that HERV ORFs represent a very interesting source of novel tumor-specific antigens with even the potential to surpass entity boundaries.The development of new tumor(immune-) therapies is a very active field and true tumor-specific targets are of outstanding interest since they minimize the risk of autoimmunity and could reduce side effects.Finally,we postulate on main future research streams in order to stimulate discussion on this hot topic.
基金Supported by the National Natural Science Foundation of China (30801254)
文摘Objective To investigate the variability of human cytomegalovirus (HCMV) UL138 open reading frame (ORF) in clinical strains. Methods HCMV UL138 ORF was amplified by polymerase chain reaction (PCR) and PCR amplification products were sequenced directly, and the data were analyzed in 19 clinical strains. Results LIL138 ORF in all 30 clinical strains was amplified successfully. Compared with that of Toledo strain, the nucleotide and amino acid sequence identifies of LIL138 ORF in all strains were 97.41% to 99.41% and 98.24% to 99.42%, respectively. All of the nucleofide mutations were substitutions. The spatial structure and post-translational modification sites of HL138 encoded proteins were conserved. The result of phylogenetic tree showed that HCMV HL138 sequence variations were not definitely related with different clinical symptoms. Conclusion HCMV UL138 ORF in clinical strains is high conservation, which might be helpful for UL138 encoded protein to play a role in latent infection of HCMV.