AIMTo analyze the research activity on liver abscess (LA) and identify the main topic clusters in the area.METHODSWe identified all documents using the medical subject heading “LA” indexed in t...AIMTo analyze the research activity on liver abscess (LA) and identify the main topic clusters in the area.METHODSWe identified all documents using the medical subject heading “LA” indexed in the MEDLINE database between 2001 and 2015. We performed a descriptive bibliometric analysis, characterizing the evolution of scientific activity, the publication types of the documents, the document categories of clinical interest (case reports, clinical trials, evaluation studies, meta-analysis, observational studies, practice guidelines and validation studies) and the geographic distribution of the research. We also carried out an analysis of networks and research clusters in order to identify the main topic areas of research.RESULTSOur search yielded a total of 1278 documents, showing a stable scientific production over the study period and a marked multidisciplinary nature. The research was dominated by case reports (65.9% of the documents analyzed). In terms of geographic distribution, researchers from the United States led in the number of signatures (n = 229), followed by those from Taiwan (n = 185), India (n = 145), Japan (n = 144), South Korea (n = 100), and China (n = 84). With regard to amebic LA, the top-producing countries were India and Mexico (n = 69 each), followed by the United States (n = 29). In the case of pyogenic LA, Taiwan Residents researchers led scientific production (n = 71), followed by the United States (n = 39) and China (n = 29). The most active areas of research in the field are diagnosis via computerized tomography scan, differential diagnosis with regard to liver cancer, treatment with antimicrobial agents, and Klebsiella infections (including bacteremia).CONCLUSIONClinical case reports associated with diagnosis and treatment are the main topic of study, highlighting the importance of this document type in advancing knowledge.展开更多
Pure fraction (92%-95%) of phagocytes (FP) and a mixture of amoebocytes(62%) and morula cells (38 %) FPMC of the holothurian Eupentacta fraudatrix (Holothuroidea, Dendrochirota) were obtained by using ficoll verograph...Pure fraction (92%-95%) of phagocytes (FP) and a mixture of amoebocytes(62%) and morula cells (38 %) FPMC of the holothurian Eupentacta fraudatrix (Holothuroidea, Dendrochirota) were obtained by using ficoll verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin of Yersinia pseudotuberculosis (TST) at different concentrations ( 0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5 fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians.展开更多
AIM:Many defense factors of the mother's colostrum or milk protect infants from intestinal, respiratory and systemic infections. In the present study, we investigated the effect of colostrum and mature human milk ...AIM:Many defense factors of the mother's colostrum or milk protect infants from intestinal, respiratory and systemic infections. In the present study, we investigated the effect of colostrum and mature human milk on E. histolytica parasites in vitro.METHODS:Samples of human milk were collected from 5 healthy lactating mothers.The medium with human milk at concentrations of 2%, 5% and 10% was obtained.RESULTS:The lethal effect of E. histolytica on the medium supplemented with different concentrations of both colostrum and mature human milk was significant during the first 30min. We also detected that the results of colostrum and mature human milk were similar. No statistically significant differences were found between same concentrations of colostrum and mature human milk at the same times.CONCLUSION:Colostrum and mature human milk have significant lethal effect on E. histolytica and protect against its infection in breast fed children.展开更多
A 54-year-old man presented with rectal pain and bleeding secondary to ulcerated,necrotic rectal and cecal masses that resembled colorectal carcinoma upon colonoscopy.These masses were later determined to be benign am...A 54-year-old man presented with rectal pain and bleeding secondary to ulcerated,necrotic rectal and cecal masses that resembled colorectal carcinoma upon colonoscopy.These masses were later determined to be benign amebomas caused by invasive Entamoeba histolytica,which regressed completely with medical therapy.In Western countries,the occurrence of invasive protozoan infection with formation of amebomas is very rare and can mistakenly masquerade as a neoplasm.Not surprisingly,there have been very few cases reported of this clinical entity within the United States.Moreover,we report a patient that had an extremely rare occurrence of two synchronous lesions,one involving the rectum and the other situated in the cecum.We review the current literature on the pathogenesis of invasive E.histolytica infection and ameboma formation,as well as management of this rare disease entity at a western medical center.展开更多
AIM:The effects of cobalt,copper,cadmium and barium ions on the cysts of Entamoeba histolyt/ca (E,histolytica), an amebic dysentery agent,cultured in Robinson medium were investigated. METHODS:E.histolytica cysts and ...AIM:The effects of cobalt,copper,cadmium and barium ions on the cysts of Entamoeba histolyt/ca (E,histolytica), an amebic dysentery agent,cultured in Robinson medium were investigated. METHODS:E.histolytica cysts and trophozoites isolated from a patient with amebiasis were cultivated in the medium, incubated at 37℃ for a period of 4 days and 40×10~4/ml amebic cysts were then transferred to a fresh medium.At the second stage,0.05,0.1 and 0.2 mM of selected metal ions were added to the medium,and the effects of these ions on parasitic reproduction compared with the control group were observed. RESULTS:It was determined that the number of living parasites in all the groups containing metal ions decreased significantly starting from 30 minutes (P<0.01).CuCl_2 showed the highest lethal effect on E.histolytica cysts,whereas the lowest lethal effect was observed with CoCl_2.It was also seen that the number of living cells was decreased as the ion concentration and exposure time were increased,and that there were no living parasites in the medium at the end of 24 h (P<0.01). CONCLUSION:It may be stated that the effect of ever- increasing contamination of the environment with metal waste materials on parasites should be investigated further.展开更多
文摘AIMTo analyze the research activity on liver abscess (LA) and identify the main topic clusters in the area.METHODSWe identified all documents using the medical subject heading “LA” indexed in the MEDLINE database between 2001 and 2015. We performed a descriptive bibliometric analysis, characterizing the evolution of scientific activity, the publication types of the documents, the document categories of clinical interest (case reports, clinical trials, evaluation studies, meta-analysis, observational studies, practice guidelines and validation studies) and the geographic distribution of the research. We also carried out an analysis of networks and research clusters in order to identify the main topic areas of research.RESULTSOur search yielded a total of 1278 documents, showing a stable scientific production over the study period and a marked multidisciplinary nature. The research was dominated by case reports (65.9% of the documents analyzed). In terms of geographic distribution, researchers from the United States led in the number of signatures (n = 229), followed by those from Taiwan (n = 185), India (n = 145), Japan (n = 144), South Korea (n = 100), and China (n = 84). With regard to amebic LA, the top-producing countries were India and Mexico (n = 69 each), followed by the United States (n = 29). In the case of pyogenic LA, Taiwan Residents researchers led scientific production (n = 71), followed by the United States (n = 39) and China (n = 29). The most active areas of research in the field are diagnosis via computerized tomography scan, differential diagnosis with regard to liver cancer, treatment with antimicrobial agents, and Klebsiella infections (including bacteremia).CONCLUSIONClinical case reports associated with diagnosis and treatment are the main topic of study, highlighting the importance of this document type in advancing knowledge.
文摘Pure fraction (92%-95%) of phagocytes (FP) and a mixture of amoebocytes(62%) and morula cells (38 %) FPMC of the holothurian Eupentacta fraudatrix (Holothuroidea, Dendrochirota) were obtained by using ficoll verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin of Yersinia pseudotuberculosis (TST) at different concentrations ( 0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5 fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians.
文摘AIM:Many defense factors of the mother's colostrum or milk protect infants from intestinal, respiratory and systemic infections. In the present study, we investigated the effect of colostrum and mature human milk on E. histolytica parasites in vitro.METHODS:Samples of human milk were collected from 5 healthy lactating mothers.The medium with human milk at concentrations of 2%, 5% and 10% was obtained.RESULTS:The lethal effect of E. histolytica on the medium supplemented with different concentrations of both colostrum and mature human milk was significant during the first 30min. We also detected that the results of colostrum and mature human milk were similar. No statistically significant differences were found between same concentrations of colostrum and mature human milk at the same times.CONCLUSION:Colostrum and mature human milk have significant lethal effect on E. histolytica and protect against its infection in breast fed children.
文摘A 54-year-old man presented with rectal pain and bleeding secondary to ulcerated,necrotic rectal and cecal masses that resembled colorectal carcinoma upon colonoscopy.These masses were later determined to be benign amebomas caused by invasive Entamoeba histolytica,which regressed completely with medical therapy.In Western countries,the occurrence of invasive protozoan infection with formation of amebomas is very rare and can mistakenly masquerade as a neoplasm.Not surprisingly,there have been very few cases reported of this clinical entity within the United States.Moreover,we report a patient that had an extremely rare occurrence of two synchronous lesions,one involving the rectum and the other situated in the cecum.We review the current literature on the pathogenesis of invasive E.histolytica infection and ameboma formation,as well as management of this rare disease entity at a western medical center.
文摘AIM:The effects of cobalt,copper,cadmium and barium ions on the cysts of Entamoeba histolyt/ca (E,histolytica), an amebic dysentery agent,cultured in Robinson medium were investigated. METHODS:E.histolytica cysts and trophozoites isolated from a patient with amebiasis were cultivated in the medium, incubated at 37℃ for a period of 4 days and 40×10~4/ml amebic cysts were then transferred to a fresh medium.At the second stage,0.05,0.1 and 0.2 mM of selected metal ions were added to the medium,and the effects of these ions on parasitic reproduction compared with the control group were observed. RESULTS:It was determined that the number of living parasites in all the groups containing metal ions decreased significantly starting from 30 minutes (P<0.01).CuCl_2 showed the highest lethal effect on E.histolytica cysts,whereas the lowest lethal effect was observed with CoCl_2.It was also seen that the number of living cells was decreased as the ion concentration and exposure time were increased,and that there were no living parasites in the medium at the end of 24 h (P<0.01). CONCLUSION:It may be stated that the effect of ever- increasing contamination of the environment with metal waste materials on parasites should be investigated further.
