陆虎 Land Rover它是全世界越野车爱好者的最爱,被称为“有史以来最棒的汽车”,“四轮驱动的美洲豹”。1947年,工程师威克斯兄弟根据二战后国内外的环境,认为有着强硬底盘,四轮驱动的多用途卡车最适合当时的需要。用简单、铝制的车体,...陆虎 Land Rover它是全世界越野车爱好者的最爱,被称为“有史以来最棒的汽车”,“四轮驱动的美洲豹”。1947年,工程师威克斯兄弟根据二战后国内外的环境,认为有着强硬底盘,四轮驱动的多用途卡车最适合当时的需要。用简单、铝制的车体,不仅具有在恶劣环境下更为耐用的特点,而且解决了缺乏钢铁的问题。英国罗孚公司根据这一设想,在1948年推出了首款命名为陆虎的农用小卡车,自此陆虎就成为四轮驱动的代名词,其传统与时尚结合的车型,豪华。展开更多
AIM： To investigate the anticancer activity of a chinese medical mixture, WRCP （warming and relieving Cold Phlegm）, on hepatocarcinoma Bel-7402 cells. METHODS： Fingerprints of WRCP, which were composed of aqueous ...AIM： To investigate the anticancer activity of a chinese medical mixture, WRCP （warming and relieving Cold Phlegm）, on hepatocarcinoma Bel-7402 cells. METHODS： Fingerprints of WRCP, which were composed of aqueous extracts of Aconitum carmichae/i, Rhizoma bolbostemmatis, Phytolacca acinosa, Panax notoginseng and Gekko swinhonis Gdenther, and aconitine, which could be isolated from Aconitum carmichaeli and have the potential toxicity, were identified by high pressure liquid chromatography. Bel-7402 cells were grown in the presence of WRCP, As203 or all-trans-retinoic acid （ATRA）. Cell proliferation and viability were determined by trypan blue stain. Apoptosis and cell cycle of Bel-7402 cells were detected by flow cytometry. Morphologic and ultrastructural variations were determined under optic and electronic microscopy. The secretion of alpha-fetoprotein and albumin was detected by radioimmunoassay. RESULTS： The average quality of aconitine is 1.15：1： 0.10 μg per 7.5 g extracts. WRCP could suppress the proliferation and viability of Bel-7402 cells. The percentage of apoptosis cells and S phase cells increased on WRCP-treated cells. Treated with WRCP, Bel-7402 cells showed ultrastructural features of differentiation. The alpha-fetoprotein secretion decreased while the albumin secretion increased （P 〈 0.001, P 〈 0.001, respectively） markedly in WRCP-treated cells. CONCLUSION： WRCP can affect the proliferation, differentiation and apoptosis of Bel-7402 cells. It can arrest cells in S phase and has strong cytotoxicity to Bel-7402 cells.展开更多
文摘陆虎 Land Rover它是全世界越野车爱好者的最爱,被称为“有史以来最棒的汽车”,“四轮驱动的美洲豹”。1947年,工程师威克斯兄弟根据二战后国内外的环境,认为有着强硬底盘,四轮驱动的多用途卡车最适合当时的需要。用简单、铝制的车体,不仅具有在恶劣环境下更为耐用的特点,而且解决了缺乏钢铁的问题。英国罗孚公司根据这一设想,在1948年推出了首款命名为陆虎的农用小卡车,自此陆虎就成为四轮驱动的代名词,其传统与时尚结合的车型,豪华。
文摘AIM： To investigate the anticancer activity of a chinese medical mixture, WRCP （warming and relieving Cold Phlegm）, on hepatocarcinoma Bel-7402 cells. METHODS： Fingerprints of WRCP, which were composed of aqueous extracts of Aconitum carmichae/i, Rhizoma bolbostemmatis, Phytolacca acinosa, Panax notoginseng and Gekko swinhonis Gdenther, and aconitine, which could be isolated from Aconitum carmichaeli and have the potential toxicity, were identified by high pressure liquid chromatography. Bel-7402 cells were grown in the presence of WRCP, As203 or all-trans-retinoic acid （ATRA）. Cell proliferation and viability were determined by trypan blue stain. Apoptosis and cell cycle of Bel-7402 cells were detected by flow cytometry. Morphologic and ultrastructural variations were determined under optic and electronic microscopy. The secretion of alpha-fetoprotein and albumin was detected by radioimmunoassay. RESULTS： The average quality of aconitine is 1.15：1： 0.10 μg per 7.5 g extracts. WRCP could suppress the proliferation and viability of Bel-7402 cells. The percentage of apoptosis cells and S phase cells increased on WRCP-treated cells. Treated with WRCP, Bel-7402 cells showed ultrastructural features of differentiation. The alpha-fetoprotein secretion decreased while the albumin secretion increased （P 〈 0.001, P 〈 0.001, respectively） markedly in WRCP-treated cells. CONCLUSION： WRCP can affect the proliferation, differentiation and apoptosis of Bel-7402 cells. It can arrest cells in S phase and has strong cytotoxicity to Bel-7402 cells.
