Lutein and lutein esters in marigold flowers was quantitatively determined by high performance chromatography （HPLC） with ODS-C 18 column.A mixture of CH3CNCD*2CH3OHCD*2CH3COOCH2CH3 with volume ratio of 55∶1∶44 wa...Lutein and lutein esters in marigold flowers was quantitatively determined by high performance chromatography （HPLC） with ODS-C 18 column.A mixture of CH3CNCD*2CH3OHCD*2CH3COOCH2CH3 with volume ratio of 55∶1∶44 was used as mobile phase at a flow rate of 1.0 mL/min and detection was carried out at 460nm. The column temperature was about 20℃. The contents of lutein and lutein esters were determined by analytical curve of lutein since lutein and lutein esters have the same spectral characteristics. Determination results of hexane extracts and saponified samples of lutein show that the saponification transforms the esters into free lutein. The increase of the content of dipalmitate and palmitate stearate reveals that the reaction includes transesterifications.展开更多
With rutin standard sample as the comparison, flavonoid extraction rate as an indicator, UV-2450 ultraviolet visible light spectrophotometer to measure flavonoid content in planted Trollius chinensis through orthogona...With rutin standard sample as the comparison, flavonoid extraction rate as an indicator, UV-2450 ultraviolet visible light spectrophotometer to measure flavonoid content in planted Trollius chinensis through orthogonal experiment, this experiment optimizes process condition of flavonoid in Trollius chinensis through water extraction and ethanol extraction. The result shows that flavonoid extraction rate of Trollius chinensis through ethanol extraction method is obviously higher than water extraction, and the optimal extraction process condition is： ethanol concentration is 75%, extraction time is 90min, extraction times are 3, and fluid material ratio is 20：1.展开更多
It takes flavonoid extraction rate as indicator and adopts the method of orthogonal experiment to study process of total flavonoid in Trollius chinensis through ultrasonic wave assisted extraction, microwave assisted ...It takes flavonoid extraction rate as indicator and adopts the method of orthogonal experiment to study process of total flavonoid in Trollius chinensis through ultrasonic wave assisted extraction, microwave assisted extraction and backflow extraction, as well as optimization of process parameter. The result indicates that in terms of extraction efficiency, microwave extraction method is better than ultrasonic radiation extraction method, which is better than backflow extraction method. Optimal process parameter through backflow extraction is： backflow temperature 60℃, fluid material ratio 1：20, ethanol density 65%, backflow time 60min; optimal process parameter through ultrasonic wave assisted extraction is： ultrasonic radiation temperature 70℃, fluid material ratio 1：20, ethanol density 65%, ultrasonic wave radiation time 45rain; optimal process parameter of microwave assisted extraction is： microwave radiation temperature 60℃, fluid material ratio 1：20, ethanol density 75%, microwave radiation time 45min.展开更多
Parkinson’s disease(PD) is a common degenerative disease of the central nervous system, and the pathologic features are mainly degeneration of substantianigra and dopamine neurons. Studies have shown that safflower f...Parkinson’s disease(PD) is a common degenerative disease of the central nervous system, and the pathologic features are mainly degeneration of substantianigra and dopamine neurons. Studies have shown that safflower flavonoid extract(SAFE) exhibits the neuroprotective effect. In this study, the safflower flavonoid extract drop pills(SAFE-DPs) for anti-PD were prepared by the heating and melting method using SAFE and matrix PEG6000. The performances of the pills were evaluated with powder X-ray diffraction(PXRD), differential scanning calorimetry(DSC), Fourier transform infrared spectroscopy(FT-IR), scanning electron microscopy(SEM) and dissolution testing. The analysis results demonstrated an amorphous state for SAFE dispersion in the matrix PEG6000 without any chemical reaction. The SAFE-DPs demonstrated acceptable chemical and physical stability irrespective of the manufacturing process and the storage period. Dissolution testing in three dissolution media(pH 1.0, pH 6.8 and pH 7.5) indicated that SAFE-DPs had excellent dissolution property. The transport of Kaempferol-3-rutinoside(K3 R) on the Caco-2 monolayer and the absorption of K3 R in situ intestinal perfusion revealed that the principal component of SAFE had a good transport and absorption capacity. Therefore, the drop pills had better release and absorption in the gastrointestinal tract, corresponding with the pharmacological and pharmacodynamic results for PD in vivo.展开更多
As an effective component of safflower,hydroxysafflor yellow A(HSYA)has the effect of promoting blood circulation,removing blood stasis,and relieving pain,and it has a certain effect on blood stasis and wind-induced P...As an effective component of safflower,hydroxysafflor yellow A(HSYA)has the effect of promoting blood circulation,removing blood stasis,and relieving pain,and it has a certain effect on blood stasis and wind-induced Parkinson‘s disease(PD).However,the current research mostly involves a single intervention mechanism,which is not conducive to the clinical transformation of this type of drugs.In the present study,rotenone was used to construct a PD cell model,and the protective effect of HSYA on the PD cell model was evaluated by cell viability and mitochondrial membrane potential.TTD database was used to query PD-related therapeutic targets,Swiss Target Prediction database to query HSYA-related targets,STRING database was used to search the gene interaction relationship of common targets,and ClueGO pathway was adopted to enrich and analyze common targets and their interaction targets,as well as to explore the comprehensive intervention mechanism.The results showed that rotenone could successfully establish the PD cell model,and HSYA had significant protective effect on PD cell model.Through the network pharmacological analysis,36 PD-related therapeutic targets and 88 HSYA-related targets were queried.The common targets of PD and HSYA were FKBP1A,HTR1A,SLC6A4 and SLC6A3.To enrich four common targets and their interaction targets through REACTOME pathway,eight cell signal pathways were obtained,and six cell biological processes were obtained through biological process pathway enrichment.展开更多
文摘Lutein and lutein esters in marigold flowers was quantitatively determined by high performance chromatography （HPLC） with ODS-C 18 column.A mixture of CH3CNCD*2CH3OHCD*2CH3COOCH2CH3 with volume ratio of 55∶1∶44 was used as mobile phase at a flow rate of 1.0 mL/min and detection was carried out at 460nm. The column temperature was about 20℃. The contents of lutein and lutein esters were determined by analytical curve of lutein since lutein and lutein esters have the same spectral characteristics. Determination results of hexane extracts and saponified samples of lutein show that the saponification transforms the esters into free lutein. The increase of the content of dipalmitate and palmitate stearate reveals that the reaction includes transesterifications.
文摘With rutin standard sample as the comparison, flavonoid extraction rate as an indicator, UV-2450 ultraviolet visible light spectrophotometer to measure flavonoid content in planted Trollius chinensis through orthogonal experiment, this experiment optimizes process condition of flavonoid in Trollius chinensis through water extraction and ethanol extraction. The result shows that flavonoid extraction rate of Trollius chinensis through ethanol extraction method is obviously higher than water extraction, and the optimal extraction process condition is： ethanol concentration is 75%, extraction time is 90min, extraction times are 3, and fluid material ratio is 20：1.
文摘It takes flavonoid extraction rate as indicator and adopts the method of orthogonal experiment to study process of total flavonoid in Trollius chinensis through ultrasonic wave assisted extraction, microwave assisted extraction and backflow extraction, as well as optimization of process parameter. The result indicates that in terms of extraction efficiency, microwave extraction method is better than ultrasonic radiation extraction method, which is better than backflow extraction method. Optimal process parameter through backflow extraction is： backflow temperature 60℃, fluid material ratio 1：20, ethanol density 65%, backflow time 60min; optimal process parameter through ultrasonic wave assisted extraction is： ultrasonic radiation temperature 70℃, fluid material ratio 1：20, ethanol density 65%, ultrasonic wave radiation time 45rain; optimal process parameter of microwave assisted extraction is： microwave radiation temperature 60℃, fluid material ratio 1：20, ethanol density 75%, microwave radiation time 45min.
基金Science and Technology Major Projects:Significant New-Drugs Creation(Grant No.2012ZX09103201-042)
文摘Parkinson’s disease(PD) is a common degenerative disease of the central nervous system, and the pathologic features are mainly degeneration of substantianigra and dopamine neurons. Studies have shown that safflower flavonoid extract(SAFE) exhibits the neuroprotective effect. In this study, the safflower flavonoid extract drop pills(SAFE-DPs) for anti-PD were prepared by the heating and melting method using SAFE and matrix PEG6000. The performances of the pills were evaluated with powder X-ray diffraction(PXRD), differential scanning calorimetry(DSC), Fourier transform infrared spectroscopy(FT-IR), scanning electron microscopy(SEM) and dissolution testing. The analysis results demonstrated an amorphous state for SAFE dispersion in the matrix PEG6000 without any chemical reaction. The SAFE-DPs demonstrated acceptable chemical and physical stability irrespective of the manufacturing process and the storage period. Dissolution testing in three dissolution media(pH 1.0, pH 6.8 and pH 7.5) indicated that SAFE-DPs had excellent dissolution property. The transport of Kaempferol-3-rutinoside(K3 R) on the Caco-2 monolayer and the absorption of K3 R in situ intestinal perfusion revealed that the principal component of SAFE had a good transport and absorption capacity. Therefore, the drop pills had better release and absorption in the gastrointestinal tract, corresponding with the pharmacological and pharmacodynamic results for PD in vivo.
基金Education Department of Hebei Province(Grant No.ZD2019107)。
文摘As an effective component of safflower,hydroxysafflor yellow A(HSYA)has the effect of promoting blood circulation,removing blood stasis,and relieving pain,and it has a certain effect on blood stasis and wind-induced Parkinson‘s disease(PD).However,the current research mostly involves a single intervention mechanism,which is not conducive to the clinical transformation of this type of drugs.In the present study,rotenone was used to construct a PD cell model,and the protective effect of HSYA on the PD cell model was evaluated by cell viability and mitochondrial membrane potential.TTD database was used to query PD-related therapeutic targets,Swiss Target Prediction database to query HSYA-related targets,STRING database was used to search the gene interaction relationship of common targets,and ClueGO pathway was adopted to enrich and analyze common targets and their interaction targets,as well as to explore the comprehensive intervention mechanism.The results showed that rotenone could successfully establish the PD cell model,and HSYA had significant protective effect on PD cell model.Through the network pharmacological analysis,36 PD-related therapeutic targets and 88 HSYA-related targets were queried.The common targets of PD and HSYA were FKBP1A,HTR1A,SLC6A4 and SLC6A3.To enrich four common targets and their interaction targets through REACTOME pathway,eight cell signal pathways were obtained,and six cell biological processes were obtained through biological process pathway enrichment.
