Background and Purpose: Therapeutic communication is a new term in family health care nursing, defined by Hohashi (2019) as a method of family intervention, and characterized by inclusion of not only verbal conversati...Background and Purpose: Therapeutic communication is a new term in family health care nursing, defined by Hohashi (2019) as a method of family intervention, and characterized by inclusion of not only verbal conversation but also nonverbal interaction. However, specific therapeutic communication methods have not been systematized. The purpose of this study was to clarify therapeutic communication methods for families/family members from the perspectives of verbal communication and non-verbal communication through a review of existing literature. Methods: We conducted a search using the medical literature databases PubMed and Ichushi-Web using the keywords “therapeutic communication”. Analysis was performed on seven articles from PubMed and 14 articles from Ichushi-Web that described therapeutic communication methods performed by healthcare professionals for families/family members. Through directed content analysis, therapeutic communication methods were subcategorized, and classified into three categories: verbal communication, non-verbal communication, and verbal/non-verbal communication. Results: A total of 23 subcategories were extracted. Verbal communication included 11 subcategories, such as “asking questions using the communicatee’s words as they are”. Non-verbal communication included five subcategories, such as “noticing changes in the content of the communicatee’s story”. And verbal/non-verbal communication featured seven subcategories, such as “making the communicatee aware of one’s own beliefs”. Conclusion: Therapeutic communication methods included basic care/caring in family interviews/meetings, as well as verbal communication and non-verbal communication that act on family/family members’ beliefs. It is believed that changes in family/family members’ beliefs can be used to eliminate, reduce, or improve problematic conditions in the family. .展开更多
目的探讨环状RNA ACAP2(circACAP2)调节微小RNA(miR)-421/B细胞易位基因2(BTG2)轴对心肌梗死(MI)大鼠心肌损伤的影响。方法构建MI大鼠模型和H9c2细胞模型,将80只大鼠分为假手术组、MI组、小干扰RNA-阴性对照(si-NC)组、si-circACAP2组、...目的探讨环状RNA ACAP2(circACAP2)调节微小RNA(miR)-421/B细胞易位基因2(BTG2)轴对心肌梗死(MI)大鼠心肌损伤的影响。方法构建MI大鼠模型和H9c2细胞模型,将80只大鼠分为假手术组、MI组、小干扰RNA-阴性对照(si-NC)组、si-circACAP2组、pcDNA3.1组、pcDNA3.1-circACAP2组、pcDNA3.1-circACAP2+模拟物(mimic)NC组、pcDNA3.1-circACAP2+miR-421 mimic组,每组各10只。将缺氧H9c2细胞置于24孔板中,瞬时转染细胞,分为缺氧组、缺氧+si-NC组、缺氧+si-circACAP2组、缺氧+pcDNA3.1组、缺氧+pcDNA3.1-circACAP2组、缺氧+pcDNA3.1-circACAP2+mimic NC组、缺氧+pcDNA3.1-circACAP2+miR-421 mimic组,另取正常培养的H9c2细胞作为对照组。检测大鼠左心室射血分数(LVEF)、左心室短轴缩短率(LVFS)、心肌梗死情况、心肌组织病理变化、circACAP2、miR-421、BTG2 mRNA表达情况、乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB)活性、H9c2细胞活力和凋亡、心肌组织BTG2蛋白表达、H9c2细胞BTG2蛋白表达。结果MI组circACAP2、BTG2 mRNA表达高于假手术组(1.84±0.21 vs 1.00±0.10,1.68±0.17 vs 1.00±0.10),miR-421表达低于假手术组(0.49±0.05 vs 1.00±0.11,P<0.05);与假手术组比较,MI组梗死面积、CK-MB、LDH活性升高,LVFS、LVEF降低(P<0.05)。与si-NC组比较,si-circACAP2组心肌损伤减轻,LVFS、LVEF升高,梗死面积、CK-MB、LDH活性降低(P<0.05);与缺氧+si-NC组比较,缺氧+si-circACAP2组细胞活力升高,凋亡率、CK-MB和LDH活性降低(P<0.05)。与pcDNA3.1组比较,pcDNA3.1-circACAP2组心肌损伤加重,LVFS、LVEF降低,梗死面积、CK-MB、LDH活性升高(P<0.05);与缺氧+pcDNA3.1组比较,缺氧+pcDNA3.1-circACAP2组细胞活力降低,凋亡率、CK-MB、LDH活性升高(P<0.05)。结论circACAP2在MI大鼠和H9c2细胞中表达上调,沉默circACAP2可能通过调节miR-421/BTG2轴改善心脏功能,减少心肌损伤,提高心肌细胞活力。展开更多
Objective Fatal familial insomnia （FFI） is an autosomal dominant prion disease characterized clinically by inattention, sleep loss, dysautonomia, and motor signs. This study is aimed to investigate clinical and fami...Objective Fatal familial insomnia （FFI） is an autosomal dominant prion disease characterized clinically by inattention, sleep loss, dysautonomia, and motor signs. This study is aimed to investigate clinical and familial characteristics often Chinese Patients with FFI. Methods We identified ten FFI cases from the surveillance network for Creutafeldt- Jakob disease （CJD） in China.Final diagnosis of FFI cases was made in accordance with the WHO criteria for CJD.The main clinical features and family histories of these ten FFI cases were analyzed. Results The median age of ten cases at onset was 38 years （from 19 to 55）. The foremost symptoms seemed to be various, including sleep disturbances, vision disorder, dizziness and anorexia. Sleep disturbances appeared in all cases and lasted in the whole clinical courses. Progressive sympathetic symptoms, memory loss, movement disturbances, myoclonus and hypertension were also frequently observed. The median duration of the disease was 9.5 months. EEG and MRI did not figure out special abnormality. 14-3-3 protein in CSF was positive in five out of eight tested patients. Clear family histories were identified in 8 patients. Conclusion The data from our study confirm that the Chinese FFI cases have similar clinical characteristics as that of the Caucasian cases. Compared with other genetic CJD associated mutations, the genetic frequencies of D178N in PRNP are apparently high among the Chinese cases.展开更多
In family healthcare nursing, the family system unit (i.e., a group in which the members, seen as a whole, mutually interact) is the target of care. As nurses tend to obtain family-related information from particular ...In family healthcare nursing, the family system unit (i.e., a group in which the members, seen as a whole, mutually interact) is the target of care. As nurses tend to obtain family-related information from particular family members in the clinical setting, when assessing families, they often confront the issue of the differences between the theoretical level and methodological level. Although this issue needs resolving for evidence-based family nursing practice, sufficient research is lacking on the methodology related to family assessment. The present study aimed to clarify the factors that affected evaluation of family functioning among couples. Semi-structured interviews were conducted with 10 child-rearing families (couples) using the Survey of Family Environment (SFE) as a family functioning scale. Content analysis identified 12 factors that affected discrepancies in the couples’ evaluations and eight factors that affected agreement in those evaluations. These factors were classified into three categories: factors concerning family or family members;factors concerning questions related to the SFE;and factors concerning the view of the family as a whole. The results of this study should contribute to the development of family assessment tools and effective methods for evaluation of family care.展开更多
Background: About 70% of Japanese children with severe motor and intellectual disabilities (SMID) live at home, and the number is increasing. Family members have an enormous burden of daily physical care for these chi...Background: About 70% of Japanese children with severe motor and intellectual disabilities (SMID) live at home, and the number is increasing. Family members have an enormous burden of daily physical care for these children. A top priority is to understand quality of life (QoL), family function, and family empowerment to effectively support these families. We aimed to assess current living situations of families with a SMID child, and to reveal the relationships between QoL, family function, and family empowerment. Methods: Sixty-five family members from 34 families with a SMID child participated in this study. We assessed 5 parameters using the Japanese versions of the following instruments: World Health Organization Quality of Life 26 (WHOQOL26), Kinder Lebensqualitats Fragebogen (KINDL), Family Assessment Device (FAD), Family Adaptability and Cohesion Evaluation Scale KG-4 (FACESKG-4), and Family Empowerment Scale (FES). Correlation and multiple regression analyses were conducted;QoL score was the objective variable. Results: Participants included 54 parents (34 mothers, 20 fathers) and 11 siblings. The mean age of SMID children was 10.4 ± 5.03 years. Twenty-two children needed multiple types of medical care. The mean age of parents and siblings was 41.5 ± 6.16 years and 15.5 ± 2.35 years, respectively. The mean QoL score (3.28 ± 0.5) was similar to the Japanese average. The mean KINDL score (77.2 ± 12.1) was higher than those of previous studies. The mean FAD score was 1.97 ± 0.32. For FACEKG-4, the score of adaptability was correlated with WHOQOL score (r = 0.459, p < 0.05). The mean score of FES was 113.6 ± 14. As the result of multiple regression analysis, lower family FAD scores ([sb] = ?0.61, p < 0.01) indicated higher family function and greater age of participants (sb = 0.495, p < 0.01) was correlated with higher WHOQOL scores (F = 15.208, p < 0.01). Conclusions: Our results indicated that the individual QoL depended on the age of participants (equals the years of experience caring for a SMID child) and the recognition of family function as a whole. Thus, to improve family members’ QoL, we should focus on individuals and also approach the family as a whole.展开更多
Objectives: The families of these children experience distress both at the time of diagnosis and afterward.A top priority is to understand family empowerment,family function,and family members' quality of life (Qo...Objectives: The families of these children experience distress both at the time of diagnosis and afterward.A top priority is to understand family empowerment,family function,and family members' quality of life (QoL) and to effectively support these families in Japan.The objective of this study was to assess the actual conditions of families living with children having DDs and to explore the factors associated with family empowerment and parents' QoL.Methods: We surveyed ninety-three parents (78 mothers,15 fathers) from 78 families which lived with children with DDs in the capital region of Japan.We assessed two main outcomes using the Japanese versions of the following instruments: Family Empowerment Scale (FES),World Health Organization Quality of Life 26 (WHOQOL26),and other six outcomes.Correlation and multiple regression analyses were conducted.Results: No medication,cooperation with child rearing,assistance from a developmental support center,solved problems related to child rearing,and higher scores in Problem Solving contributed to higher FES scores.Higher WHOQOL26 scores were related to being a full-time housewife,higher self-esteem,no developmental support,a broad emotional support network,higher scores in Problem Solving and Role Function,and lower scores in Affective Reaction and General Function.Conclusions: We revealed that family empowerment and QoL of parents rearing children with DDs in Japan were affected by various subscales of family function and other family attributes.Effective interventions for improving family empowerment and QoL should be researched in the future.展开更多
This paper reports a familial amyloid polyneuropathy (FAP) family in China. This family being investigated had 69 members of five generations. From the third generation, there have been 16 patients. The age of onset w...This paper reports a familial amyloid polyneuropathy (FAP) family in China. This family being investigated had 69 members of five generations. From the third generation, there have been 16 patients. The age of onset was about 3 to 5 decades. The initial symptoms were autonomic nerve symptoms, such as impotence, dyspepsia and diarrhoea, associated with the sensory loss of lower extremities. As the disease progressed, the upper extremities and motor ability were also involved. The duration of disease course was about 8-10 years, most patients died of infection and cachexia. Sural biopsy in 3 patients had showed positive Congo red staining. From the clinical view, this FAP family is similar to FAP I found in Japan. The true classification, however, should be confirmed by further genetic analysis.展开更多
Objectives:Family caregivers raising children with severe motor and intellectual disabilities(SMID)experience the enormous burden of care.The concept of family empowerment is one of the important assessment indexes of...Objectives:Family caregivers raising children with severe motor and intellectual disabilities(SMID)experience the enormous burden of care.The concept of family empowerment is one of the important assessment indexes of family nursing from the perspective of providing comprehensive support for these families.The objective of this study was to identify the factors associated with the empowerment of families raising a child with SMID in Japan.Methods:We conducted a nationwide questionnaire survey involving 1659 primary caregivers raising a child with SMID through 89 special schools.We assessed the main outcomes using the Family Empowerment Scale(FES).We then conducted a multiple linear regression analysis to reveal the factors associated with family empowerment.Results:In total,1362 primary caregivers were included in our study.Our results show that factors contributing to high FES scores are higher age of the primary caregiver,higher education,greater recognition of regional support,lower childcare burden,higher utilization of home visit services,higher usage of a childcare institution,higher household income,and stronger family bonding.Conclusion:Healthcare professionals should carefully assess the state of family empowerment of the primary caregivers who are younger and those who have low education,low household income,high childcare burden,and fragile bonding with the family.Second,they should encourage such families to use regional support resources for childcare.That is,policy makers should consider ways to promote home visits and institutional services for the care of children with SMID,aiming especially for the provision of well-coordinated care and services.展开更多
Dilated cardiomyopathy（DCM）is characterized by the dilated heart chambers and reduced systolic function in the absence of specific aetiology[1].Approximately one third of DCM cases are hereditary.In recent years,DCM...Dilated cardiomyopathy（DCM）is characterized by the dilated heart chambers and reduced systolic function in the absence of specific aetiology[1].Approximately one third of DCM cases are hereditary.In recent years,DCM concomitant with arrhythmias and sudden death resulting from gene mutation has been widely展开更多
In recent years in China,family education has become more and more important.Family plays the most crucial role in children's education.So more and more parents begin to pay much more attention to family education...In recent years in China,family education has become more and more important.Family plays the most crucial role in children's education.So more and more parents begin to pay much more attention to family education than before.However,what ways are best for them to use?Most parents have no idea at all and some are trying to use some education methods learned from books.Little Women is a book from which we can learn a lot of useful things,the obvious one is family education.This novel was written by an American author in 19th century.As a result,both time and place are a little far away from people in China.But at that time,the March's family education did work very well and made far-reaching influence.So in the first part in this paper what will be introduced in is about finding out how the March's family dealt with those unpleasant things,and the second part will discuss some disadvantages of Chinese family education,then the third part will see if March's ways could be used in Chinese families or how to make some changes about their ways so that those ways could work in Chinese families as well as they did in the March's.展开更多
This paper explores the influences of Emotional Regulation (ER) and work schedules on work-family conflict (WFC) among Italian nurses, also accounting for some familial variables. The data used in this study come from...This paper explores the influences of Emotional Regulation (ER) and work schedules on work-family conflict (WFC) among Italian nurses, also accounting for some familial variables. The data used in this study come from a survey conducted on 191 nurses working in two public hospitals of Tuscany (Italy). Stepwise multiple regressions were applied to examine the relationships among these variables, using the WFC as dependent variable. We found that some work related dimensions had direct effects on WFC outcomes;however, these impacts on the criterion variables are modified by the effects exerted by specific ER strategies.展开更多
Background and Aims: A comprehensive family health history still offers the best data for calculating risk of most complex diseases, but lack of informant accuracy hinders its use. Young adults transitioning from the ...Background and Aims: A comprehensive family health history still offers the best data for calculating risk of most complex diseases, but lack of informant accuracy hinders its use. Young adults transitioning from the parental home to more independent university life may be poor historians. The purpose of this study was to describe the quality of family health information acquired by young university students and to characterize the process by which they learn family health facts. Methods: Thematic content analysis was applied to interviews with undergraduate students enrolled in diverse classes across disciplines at a public US university. Results: Two processes, hanging around and trickling down, described the ways young adults learned family health information. The majority of respondents described both empowerment and a sense of vulnerability that resulted from having their family health information. Conclusions: Family health history provided by young adult students is characterized by inaccuracies related to lack of information and misunderstanding of information that is available. Young students are typically transitioning from the care of local primary care physicians into relationships with new healthcare providers, and are interested in family health history. Thus, this transition is an opportunity for nurses to construct an organized family health history with them.展开更多
Families need sufficient learning opportunities about mental illness. Therefore, family peer education program has been developed in Japan following the U.S. and Hong Kong. We aimed to identify program components that...Families need sufficient learning opportunities about mental illness. Therefore, family peer education program has been developed in Japan following the U.S. and Hong Kong. We aimed to identify program components that families recognize which would provide suggestions in modifying the program. We used qualitative and inductive study design. Data were collected through group interviews for six family research participants. We coded the data to focus on relationships between program components, program processes, and impacts. Data Analysis resulted in seven assumptions based on causal relationships and three types of program components: program form, learning style and facilitation skills. The program form is family-led peer group, closed-membership, 5 times per course, and small group. The learning style is combination of textbook and experiential knowledge, family peer learning, and sharing experiences. The facilitation skills are group work focusing on strengths of family members, the acceptance of emotional disclosure, omotenashi, and teamwork skills.展开更多
Background: A previous genome-wide linkage study of alcohol dependence in multiplex families found a suggestive linkage result for a region on Chromosome 1 near microsatellite markers D1S196 and D1S2878. The KIAA0040 ...Background: A previous genome-wide linkage study of alcohol dependence in multiplex families found a suggestive linkage result for a region on Chromosome 1 near microsatellite markers D1S196 and D1S2878. The KIAA0040 gene has been mapped to this region (1q24-q25). A recent genome-wide association study using SAGE (the Study of Addiction: Genetics and Environment) and COGA (Collaborative Study on the Genetics of Alcoholism) found five SNPs within the KIAA0040 gene significantly associated with alcohol dependence. A meta-analysis using data from these sources also found the KIAA0040 gene significantly associated with alcohol dependence. Methods: Using family data consisting of 1000 individuals with phenotypic data (762 with both phenotype and DNA), finer mapping of a 0.3 cM region that included the KIAA0040 gene and a flanking gene was undertaken using SNPs with minor allele frequency (MAF) ≥ 0.15 and pair-wise linkage disequilibrium (LD) of r2 < 0.8 using the HapMap CEU population. Results: Significant FBAT p-values were observed for six SNPs, four within the KIAA0040 gene (rs2269650, rs2861158, rs1008459, rs2272785) and two adjacent to KIAA0040 (rs10912899 and rs3753555). Five haplotype blocks of varying size were identified using HAPLOVIEW. Analysis using the haplotype-based test function of FBAT revealed one two-SNP block (rs1008459rs2272785) associated with alcohol dependence. This block showed a pattern of transmission in which one haplotype, CT, with a frequency of 0.577 was found to be over-transmitted to affected offspring (p = 0.017) while another haplotype, AG, with a frequency of 0.238 was found to be under-transmitted to affected offspring (p = 0.006). A three-SNP block (rs1008459rs2272785-rs375355) showed an overall significant association (p = 0.011) with alcohol dependence with the haplotype ACT over-transmitted to affected offspring (p = 0.016) and the haplotype GAG undertransmitted (p = 0.002). Conclusions: Family-based association analysis shows the KIAA0040 gene significantly associated with alcohol dependence. The potential importance of the KIAA0040 gene for AD risk is currently unknown. However, the present results support earlier findings from a genome-wide association study.展开更多
Background: It is well established that the people with elevated SBP, DBP, BMI and WHR are more prone to cardiovascular disease. However, very few studies have focused on the amount of familial aggregation and heritab...Background: It is well established that the people with elevated SBP, DBP, BMI and WHR are more prone to cardiovascular disease. However, very few studies have focused on the amount of familial aggregation and heritability of these cardiovascular risk factors in Indian population. Therefore, purpose of this study was to investigate the familial aggregation of blood pressures with respect to certain anthropometric traits and to determine the relative roles of heredity in the etiology of SBP and DBP in a sample of families with three generations. Methods: The study has been conducted through house to house family study among three generations such as offspring, parent and grandparent in a scheduled caste community (Ramdasia) in Punjab. A total of 1400 individuals, constituting 380 families were surveyed for blood pressure, pulse rate, pulse pressure and anthropometric measurements to study familial aggregation and heritability for cardiovascular risk factors. The analysis represents a multivariate model which includes the each individual family data for estimation of familial correlation and heritability. Results: All risk factors showed positive familial correlation but magnitudes are different in va rious pairs of combination. Correlations generally are higher among genetically close relatives such as brothersisters or parentoffspring and are lower among spouses. The estimated heri tabilities were 22% for systolic and 27% for diastolic blood pressure, 19% for BMI and 17% for WHR. Conclusions: These results indicate a strong familial aggregation of cardiovascular risk factors such as SBP and DBP in this population and also showed that this familial influence can be detected from anthropometric mea surements and genetic closeness. Almost all anthropometric variables were found to be significant with blood pressures among three ge nerations.展开更多
Kidney Renal Clear Cell Carcinoma(KIRC)is a malignant tumor that carries a substantial risk of morbidity and mortality.The MMP family assumes a crucial role in tumor invasion and metastasis.This study aimed to uncover...Kidney Renal Clear Cell Carcinoma(KIRC)is a malignant tumor that carries a substantial risk of morbidity and mortality.The MMP family assumes a crucial role in tumor invasion and metastasis.This study aimed to uncover the mechanistic relevance of the MMP gene family as a therapeutic target and diagnostic biomarker in Kidney Renal Clear Cell Carcinoma(KIRC)through a comprehensive approach encompassing both computational and molecular analyses.STRING,Cytoscape,UALCAN,GEPIA,OncoDB,HPA,cBioPortal,GSEA,TIMER,ENCORI,DrugBank,targeted bisulfite sequencing(bisulfite-seq),conventional PCR,Sanger sequencing,and RT-qPCR based analyses were used in the present study to analyze MMP gene family members to accurately determine a few hub genes that can be utilized as both therapeutic targets and diagnostic biomarkers for KIRC.By performing STRING and Cytohubba analyses of the 24 MMP gene family members,MMP2(matrix metallopeptidase 2),MMP9(matrix metallopeptidase 9),MMP12(matrix metallopeptidase 12),and MMP16(matrix metallopeptidase 16)genes were denoted as hub genes having highest degree scores.After analyzing MMP2,MMP9,MMP12,and MMP16 via various TCGA databases and RT-qPCR technique across clinical samples and KIRC cell lines,interestingly,all these hub genes were found significantly overexpressed at mRNA and protein levels in KIRC samples relative to controls.The notable effect of the up-regulated MMP2,MMP9,MMP12,and MMP16 was also documented on the overall survival(OS)of the KIRC patients.Moreover,targeted bisulfite-sequencing(bisulfite-seq)analysis revealed that promoter hypomethylation pattern was associated with up-regulation of hub genes(MMP2,MMP9,MMP12,and MMP16).In addition to this,hub genes were involved in various diverse oncogenic pathways.The MMP gene family members(MMP2,MMP9,MMP12,and MMP16)may serve as therapeutic targets and prognostic biomarkers in KIRC.展开更多
文摘Background and Purpose: Therapeutic communication is a new term in family health care nursing, defined by Hohashi (2019) as a method of family intervention, and characterized by inclusion of not only verbal conversation but also nonverbal interaction. However, specific therapeutic communication methods have not been systematized. The purpose of this study was to clarify therapeutic communication methods for families/family members from the perspectives of verbal communication and non-verbal communication through a review of existing literature. Methods: We conducted a search using the medical literature databases PubMed and Ichushi-Web using the keywords “therapeutic communication”. Analysis was performed on seven articles from PubMed and 14 articles from Ichushi-Web that described therapeutic communication methods performed by healthcare professionals for families/family members. Through directed content analysis, therapeutic communication methods were subcategorized, and classified into three categories: verbal communication, non-verbal communication, and verbal/non-verbal communication. Results: A total of 23 subcategories were extracted. Verbal communication included 11 subcategories, such as “asking questions using the communicatee’s words as they are”. Non-verbal communication included five subcategories, such as “noticing changes in the content of the communicatee’s story”. And verbal/non-verbal communication featured seven subcategories, such as “making the communicatee aware of one’s own beliefs”. Conclusion: Therapeutic communication methods included basic care/caring in family interviews/meetings, as well as verbal communication and non-verbal communication that act on family/family members’ beliefs. It is believed that changes in family/family members’ beliefs can be used to eliminate, reduce, or improve problematic conditions in the family. .
文摘目的探讨环状RNA ACAP2(circACAP2)调节微小RNA(miR)-421/B细胞易位基因2(BTG2)轴对心肌梗死(MI)大鼠心肌损伤的影响。方法构建MI大鼠模型和H9c2细胞模型,将80只大鼠分为假手术组、MI组、小干扰RNA-阴性对照(si-NC)组、si-circACAP2组、pcDNA3.1组、pcDNA3.1-circACAP2组、pcDNA3.1-circACAP2+模拟物(mimic)NC组、pcDNA3.1-circACAP2+miR-421 mimic组,每组各10只。将缺氧H9c2细胞置于24孔板中,瞬时转染细胞,分为缺氧组、缺氧+si-NC组、缺氧+si-circACAP2组、缺氧+pcDNA3.1组、缺氧+pcDNA3.1-circACAP2组、缺氧+pcDNA3.1-circACAP2+mimic NC组、缺氧+pcDNA3.1-circACAP2+miR-421 mimic组,另取正常培养的H9c2细胞作为对照组。检测大鼠左心室射血分数(LVEF)、左心室短轴缩短率(LVFS)、心肌梗死情况、心肌组织病理变化、circACAP2、miR-421、BTG2 mRNA表达情况、乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB)活性、H9c2细胞活力和凋亡、心肌组织BTG2蛋白表达、H9c2细胞BTG2蛋白表达。结果MI组circACAP2、BTG2 mRNA表达高于假手术组(1.84±0.21 vs 1.00±0.10,1.68±0.17 vs 1.00±0.10),miR-421表达低于假手术组(0.49±0.05 vs 1.00±0.11,P<0.05);与假手术组比较,MI组梗死面积、CK-MB、LDH活性升高,LVFS、LVEF降低(P<0.05)。与si-NC组比较,si-circACAP2组心肌损伤减轻,LVFS、LVEF升高,梗死面积、CK-MB、LDH活性降低(P<0.05);与缺氧+si-NC组比较,缺氧+si-circACAP2组细胞活力升高,凋亡率、CK-MB和LDH活性降低(P<0.05)。与pcDNA3.1组比较,pcDNA3.1-circACAP2组心肌损伤加重,LVFS、LVEF降低,梗死面积、CK-MB、LDH活性升高(P<0.05);与缺氧+pcDNA3.1组比较,缺氧+pcDNA3.1-circACAP2组细胞活力降低,凋亡率、CK-MB、LDH活性升高(P<0.05)。结论circACAP2在MI大鼠和H9c2细胞中表达上调,沉默circACAP2可能通过调节miR-421/BTG2轴改善心脏功能,减少心肌损伤,提高心肌细胞活力。
基金supported by China Mega-Project for Infectious Disease(2009ZX10004-101,2008ZX)SKLID Development Grant(2008SKLID102,2011SKLID211)+3 种基金National Basic Research Program of China(973 Program)(2007CB310505)sponsored by the Young Scholar Scientific Research Foundation of China CDC(2012A102)Chinese National Natural Science Foundation Grants 30771914 and 30800975 Institution Technique R&D Grant(2008EG150300)
文摘Objective Fatal familial insomnia （FFI） is an autosomal dominant prion disease characterized clinically by inattention, sleep loss, dysautonomia, and motor signs. This study is aimed to investigate clinical and familial characteristics often Chinese Patients with FFI. Methods We identified ten FFI cases from the surveillance network for Creutafeldt- Jakob disease （CJD） in China.Final diagnosis of FFI cases was made in accordance with the WHO criteria for CJD.The main clinical features and family histories of these ten FFI cases were analyzed. Results The median age of ten cases at onset was 38 years （from 19 to 55）. The foremost symptoms seemed to be various, including sleep disturbances, vision disorder, dizziness and anorexia. Sleep disturbances appeared in all cases and lasted in the whole clinical courses. Progressive sympathetic symptoms, memory loss, movement disturbances, myoclonus and hypertension were also frequently observed. The median duration of the disease was 9.5 months. EEG and MRI did not figure out special abnormality. 14-3-3 protein in CSF was positive in five out of eight tested patients. Clear family histories were identified in 8 patients. Conclusion The data from our study confirm that the Chinese FFI cases have similar clinical characteristics as that of the Caucasian cases. Compared with other genetic CJD associated mutations, the genetic frequencies of D178N in PRNP are apparently high among the Chinese cases.
文摘In family healthcare nursing, the family system unit (i.e., a group in which the members, seen as a whole, mutually interact) is the target of care. As nurses tend to obtain family-related information from particular family members in the clinical setting, when assessing families, they often confront the issue of the differences between the theoretical level and methodological level. Although this issue needs resolving for evidence-based family nursing practice, sufficient research is lacking on the methodology related to family assessment. The present study aimed to clarify the factors that affected evaluation of family functioning among couples. Semi-structured interviews were conducted with 10 child-rearing families (couples) using the Survey of Family Environment (SFE) as a family functioning scale. Content analysis identified 12 factors that affected discrepancies in the couples’ evaluations and eight factors that affected agreement in those evaluations. These factors were classified into three categories: factors concerning family or family members;factors concerning questions related to the SFE;and factors concerning the view of the family as a whole. The results of this study should contribute to the development of family assessment tools and effective methods for evaluation of family care.
文摘Background: About 70% of Japanese children with severe motor and intellectual disabilities (SMID) live at home, and the number is increasing. Family members have an enormous burden of daily physical care for these children. A top priority is to understand quality of life (QoL), family function, and family empowerment to effectively support these families. We aimed to assess current living situations of families with a SMID child, and to reveal the relationships between QoL, family function, and family empowerment. Methods: Sixty-five family members from 34 families with a SMID child participated in this study. We assessed 5 parameters using the Japanese versions of the following instruments: World Health Organization Quality of Life 26 (WHOQOL26), Kinder Lebensqualitats Fragebogen (KINDL), Family Assessment Device (FAD), Family Adaptability and Cohesion Evaluation Scale KG-4 (FACESKG-4), and Family Empowerment Scale (FES). Correlation and multiple regression analyses were conducted;QoL score was the objective variable. Results: Participants included 54 parents (34 mothers, 20 fathers) and 11 siblings. The mean age of SMID children was 10.4 ± 5.03 years. Twenty-two children needed multiple types of medical care. The mean age of parents and siblings was 41.5 ± 6.16 years and 15.5 ± 2.35 years, respectively. The mean QoL score (3.28 ± 0.5) was similar to the Japanese average. The mean KINDL score (77.2 ± 12.1) was higher than those of previous studies. The mean FAD score was 1.97 ± 0.32. For FACEKG-4, the score of adaptability was correlated with WHOQOL score (r = 0.459, p < 0.05). The mean score of FES was 113.6 ± 14. As the result of multiple regression analysis, lower family FAD scores ([sb] = ?0.61, p < 0.01) indicated higher family function and greater age of participants (sb = 0.495, p < 0.01) was correlated with higher WHOQOL scores (F = 15.208, p < 0.01). Conclusions: Our results indicated that the individual QoL depended on the age of participants (equals the years of experience caring for a SMID child) and the recognition of family function as a whole. Thus, to improve family members’ QoL, we should focus on individuals and also approach the family as a whole.
基金This study was funded by the Ministry of Education,Culture,Sports,Science,and Technology of Japan (Grant-in-Aid for Young Scientists (A),2014-2018,No.26713057)
文摘Objectives: The families of these children experience distress both at the time of diagnosis and afterward.A top priority is to understand family empowerment,family function,and family members' quality of life (QoL) and to effectively support these families in Japan.The objective of this study was to assess the actual conditions of families living with children having DDs and to explore the factors associated with family empowerment and parents' QoL.Methods: We surveyed ninety-three parents (78 mothers,15 fathers) from 78 families which lived with children with DDs in the capital region of Japan.We assessed two main outcomes using the Japanese versions of the following instruments: Family Empowerment Scale (FES),World Health Organization Quality of Life 26 (WHOQOL26),and other six outcomes.Correlation and multiple regression analyses were conducted.Results: No medication,cooperation with child rearing,assistance from a developmental support center,solved problems related to child rearing,and higher scores in Problem Solving contributed to higher FES scores.Higher WHOQOL26 scores were related to being a full-time housewife,higher self-esteem,no developmental support,a broad emotional support network,higher scores in Problem Solving and Role Function,and lower scores in Affective Reaction and General Function.Conclusions: We revealed that family empowerment and QoL of parents rearing children with DDs in Japan were affected by various subscales of family function and other family attributes.Effective interventions for improving family empowerment and QoL should be researched in the future.
文摘This paper reports a familial amyloid polyneuropathy (FAP) family in China. This family being investigated had 69 members of five generations. From the third generation, there have been 16 patients. The age of onset was about 3 to 5 decades. The initial symptoms were autonomic nerve symptoms, such as impotence, dyspepsia and diarrhoea, associated with the sensory loss of lower extremities. As the disease progressed, the upper extremities and motor ability were also involved. The duration of disease course was about 8-10 years, most patients died of infection and cachexia. Sural biopsy in 3 patients had showed positive Congo red staining. From the clinical view, this FAP family is similar to FAP I found in Japan. The true classification, however, should be confirmed by further genetic analysis.
基金This study was funded by Grant-in-Aid for Scientific Research(15K15846 and 18H03093).
文摘Objectives:Family caregivers raising children with severe motor and intellectual disabilities(SMID)experience the enormous burden of care.The concept of family empowerment is one of the important assessment indexes of family nursing from the perspective of providing comprehensive support for these families.The objective of this study was to identify the factors associated with the empowerment of families raising a child with SMID in Japan.Methods:We conducted a nationwide questionnaire survey involving 1659 primary caregivers raising a child with SMID through 89 special schools.We assessed the main outcomes using the Family Empowerment Scale(FES).We then conducted a multiple linear regression analysis to reveal the factors associated with family empowerment.Results:In total,1362 primary caregivers were included in our study.Our results show that factors contributing to high FES scores are higher age of the primary caregiver,higher education,greater recognition of regional support,lower childcare burden,higher utilization of home visit services,higher usage of a childcare institution,higher household income,and stronger family bonding.Conclusion:Healthcare professionals should carefully assess the state of family empowerment of the primary caregivers who are younger and those who have low education,low household income,high childcare burden,and fragile bonding with the family.Second,they should encourage such families to use regional support resources for childcare.That is,policy makers should consider ways to promote home visits and institutional services for the care of children with SMID,aiming especially for the provision of well-coordinated care and services.
基金the funds of "the Youth Fund of Nantong Health Bureau 2015",ID:WQ2015009
文摘Dilated cardiomyopathy（DCM）is characterized by the dilated heart chambers and reduced systolic function in the absence of specific aetiology[1].Approximately one third of DCM cases are hereditary.In recent years,DCM concomitant with arrhythmias and sudden death resulting from gene mutation has been widely
文摘In recent years in China,family education has become more and more important.Family plays the most crucial role in children's education.So more and more parents begin to pay much more attention to family education than before.However,what ways are best for them to use?Most parents have no idea at all and some are trying to use some education methods learned from books.Little Women is a book from which we can learn a lot of useful things,the obvious one is family education.This novel was written by an American author in 19th century.As a result,both time and place are a little far away from people in China.But at that time,the March's family education did work very well and made far-reaching influence.So in the first part in this paper what will be introduced in is about finding out how the March's family dealt with those unpleasant things,and the second part will discuss some disadvantages of Chinese family education,then the third part will see if March's ways could be used in Chinese families or how to make some changes about their ways so that those ways could work in Chinese families as well as they did in the March's.
文摘This paper explores the influences of Emotional Regulation (ER) and work schedules on work-family conflict (WFC) among Italian nurses, also accounting for some familial variables. The data used in this study come from a survey conducted on 191 nurses working in two public hospitals of Tuscany (Italy). Stepwise multiple regressions were applied to examine the relationships among these variables, using the WFC as dependent variable. We found that some work related dimensions had direct effects on WFC outcomes;however, these impacts on the criterion variables are modified by the effects exerted by specific ER strategies.
文摘Background and Aims: A comprehensive family health history still offers the best data for calculating risk of most complex diseases, but lack of informant accuracy hinders its use. Young adults transitioning from the parental home to more independent university life may be poor historians. The purpose of this study was to describe the quality of family health information acquired by young university students and to characterize the process by which they learn family health facts. Methods: Thematic content analysis was applied to interviews with undergraduate students enrolled in diverse classes across disciplines at a public US university. Results: Two processes, hanging around and trickling down, described the ways young adults learned family health information. The majority of respondents described both empowerment and a sense of vulnerability that resulted from having their family health information. Conclusions: Family health history provided by young adult students is characterized by inaccuracies related to lack of information and misunderstanding of information that is available. Young students are typically transitioning from the care of local primary care physicians into relationships with new healthcare providers, and are interested in family health history. Thus, this transition is an opportunity for nurses to construct an organized family health history with them.
文摘Families need sufficient learning opportunities about mental illness. Therefore, family peer education program has been developed in Japan following the U.S. and Hong Kong. We aimed to identify program components that families recognize which would provide suggestions in modifying the program. We used qualitative and inductive study design. Data were collected through group interviews for six family research participants. We coded the data to focus on relationships between program components, program processes, and impacts. Data Analysis resulted in seven assumptions based on causal relationships and three types of program components: program form, learning style and facilitation skills. The program form is family-led peer group, closed-membership, 5 times per course, and small group. The learning style is combination of textbook and experiential knowledge, family peer learning, and sharing experiences. The facilitation skills are group work focusing on strengths of family members, the acceptance of emotional disclosure, omotenashi, and teamwork skills.
文摘Background: A previous genome-wide linkage study of alcohol dependence in multiplex families found a suggestive linkage result for a region on Chromosome 1 near microsatellite markers D1S196 and D1S2878. The KIAA0040 gene has been mapped to this region (1q24-q25). A recent genome-wide association study using SAGE (the Study of Addiction: Genetics and Environment) and COGA (Collaborative Study on the Genetics of Alcoholism) found five SNPs within the KIAA0040 gene significantly associated with alcohol dependence. A meta-analysis using data from these sources also found the KIAA0040 gene significantly associated with alcohol dependence. Methods: Using family data consisting of 1000 individuals with phenotypic data (762 with both phenotype and DNA), finer mapping of a 0.3 cM region that included the KIAA0040 gene and a flanking gene was undertaken using SNPs with minor allele frequency (MAF) ≥ 0.15 and pair-wise linkage disequilibrium (LD) of r2 < 0.8 using the HapMap CEU population. Results: Significant FBAT p-values were observed for six SNPs, four within the KIAA0040 gene (rs2269650, rs2861158, rs1008459, rs2272785) and two adjacent to KIAA0040 (rs10912899 and rs3753555). Five haplotype blocks of varying size were identified using HAPLOVIEW. Analysis using the haplotype-based test function of FBAT revealed one two-SNP block (rs1008459rs2272785) associated with alcohol dependence. This block showed a pattern of transmission in which one haplotype, CT, with a frequency of 0.577 was found to be over-transmitted to affected offspring (p = 0.017) while another haplotype, AG, with a frequency of 0.238 was found to be under-transmitted to affected offspring (p = 0.006). A three-SNP block (rs1008459rs2272785-rs375355) showed an overall significant association (p = 0.011) with alcohol dependence with the haplotype ACT over-transmitted to affected offspring (p = 0.016) and the haplotype GAG undertransmitted (p = 0.002). Conclusions: Family-based association analysis shows the KIAA0040 gene significantly associated with alcohol dependence. The potential importance of the KIAA0040 gene for AD risk is currently unknown. However, the present results support earlier findings from a genome-wide association study.
文摘Background: It is well established that the people with elevated SBP, DBP, BMI and WHR are more prone to cardiovascular disease. However, very few studies have focused on the amount of familial aggregation and heritability of these cardiovascular risk factors in Indian population. Therefore, purpose of this study was to investigate the familial aggregation of blood pressures with respect to certain anthropometric traits and to determine the relative roles of heredity in the etiology of SBP and DBP in a sample of families with three generations. Methods: The study has been conducted through house to house family study among three generations such as offspring, parent and grandparent in a scheduled caste community (Ramdasia) in Punjab. A total of 1400 individuals, constituting 380 families were surveyed for blood pressure, pulse rate, pulse pressure and anthropometric measurements to study familial aggregation and heritability for cardiovascular risk factors. The analysis represents a multivariate model which includes the each individual family data for estimation of familial correlation and heritability. Results: All risk factors showed positive familial correlation but magnitudes are different in va rious pairs of combination. Correlations generally are higher among genetically close relatives such as brothersisters or parentoffspring and are lower among spouses. The estimated heri tabilities were 22% for systolic and 27% for diastolic blood pressure, 19% for BMI and 17% for WHR. Conclusions: These results indicate a strong familial aggregation of cardiovascular risk factors such as SBP and DBP in this population and also showed that this familial influence can be detected from anthropometric mea surements and genetic closeness. Almost all anthropometric variables were found to be significant with blood pressures among three ge nerations.
基金The authors would like to extend their sincere appreciation to the Researchers Supporting Project Number(RSP2023R457),King Saud University,Riyadh,Saudi Arabia.
文摘Kidney Renal Clear Cell Carcinoma(KIRC)is a malignant tumor that carries a substantial risk of morbidity and mortality.The MMP family assumes a crucial role in tumor invasion and metastasis.This study aimed to uncover the mechanistic relevance of the MMP gene family as a therapeutic target and diagnostic biomarker in Kidney Renal Clear Cell Carcinoma(KIRC)through a comprehensive approach encompassing both computational and molecular analyses.STRING,Cytoscape,UALCAN,GEPIA,OncoDB,HPA,cBioPortal,GSEA,TIMER,ENCORI,DrugBank,targeted bisulfite sequencing(bisulfite-seq),conventional PCR,Sanger sequencing,and RT-qPCR based analyses were used in the present study to analyze MMP gene family members to accurately determine a few hub genes that can be utilized as both therapeutic targets and diagnostic biomarkers for KIRC.By performing STRING and Cytohubba analyses of the 24 MMP gene family members,MMP2(matrix metallopeptidase 2),MMP9(matrix metallopeptidase 9),MMP12(matrix metallopeptidase 12),and MMP16(matrix metallopeptidase 16)genes were denoted as hub genes having highest degree scores.After analyzing MMP2,MMP9,MMP12,and MMP16 via various TCGA databases and RT-qPCR technique across clinical samples and KIRC cell lines,interestingly,all these hub genes were found significantly overexpressed at mRNA and protein levels in KIRC samples relative to controls.The notable effect of the up-regulated MMP2,MMP9,MMP12,and MMP16 was also documented on the overall survival(OS)of the KIRC patients.Moreover,targeted bisulfite-sequencing(bisulfite-seq)analysis revealed that promoter hypomethylation pattern was associated with up-regulation of hub genes(MMP2,MMP9,MMP12,and MMP16).In addition to this,hub genes were involved in various diverse oncogenic pathways.The MMP gene family members(MMP2,MMP9,MMP12,and MMP16)may serve as therapeutic targets and prognostic biomarkers in KIRC.
