Wearable sensing systems have been designed to monitor health conditions in real-time by detecting analytes in human biofluids.Wound diagnosis remains challenging,necessitating suitable materials for high-performance ...Wearable sensing systems have been designed to monitor health conditions in real-time by detecting analytes in human biofluids.Wound diagnosis remains challenging,necessitating suitable materials for high-performance wearable sensors to offer prompt feedback.Existing devices have limitations in measuring pH and the concentration of pH-dependent electroactive species simultaneously,which is crucial for obtaining a comprehensive understanding of wound status and optimizing biosensors.Therefore,improving materials and analysis system accuracy is essential.This article introduces the first example of a flexible array capable of detecting pyocyanin,a bacterial virulence factor,while correcting dynamic pH fluctuations.We demonstrate that this combined sensor enhances accuracy by mitigating the impact of pH variability on pyocyanin sensor response.Customized screen-printable inks were developed to enhance analytical performance.The analytical performances of two sensitive sensor systems(i.e.,fully-printed porous graphene/multiwalled carbon nanotube(CNT)and polyaniline/CNT composites for pyocyanin and pH sensors)are evaluated.Partial least square regression is employed to analyze nonzero-order data arrays from square wave voltammetric and potentiometric measurements of pyocyanin and pH sensors to establish a predictive model for pyocyanin concentration in complex fluids.This sensitive and effective strategy shows potential for personalized applications due to its affordability,ease of use,and ability to adjust for dynamic pH changes.展开更多
[目的]探讨鸡热休克蛋白8(heat shock protein family A member 8,HSPA8)的生物学特性及其在应激状态下胸腺中的表达模式。[方法]试验以固始鸡胸腺为材料,利用PCR技术扩增并克隆HSPA8基因CDS区,通过生物信息学方法分析其生物学特性和结...[目的]探讨鸡热休克蛋白8(heat shock protein family A member 8,HSPA8)的生物学特性及其在应激状态下胸腺中的表达模式。[方法]试验以固始鸡胸腺为材料,利用PCR技术扩增并克隆HSPA8基因CDS区,通过生物信息学方法分析其生物学特性和结构特点,并检测其在不同组织中的相对表达量。构建断喙应激和热应激模型,采用实时荧光定量PCR技术检测炎症因子和HSPA8基因在不同应激模型鸡胸腺中的表达情况。[结果]鸡HSPA8基因CDS区长度为1 941 bp,共编码氨基酸646个,与红原鸡基因序列相似性为98.92%;HSPA8基因核苷酸序列的系统发育树结果表明,固始鸡与火鸡亲缘关系最近,与哺乳动物次之,与低等鱼类亲缘关系最远;HSPA8蛋白属于酸性蛋白,稳定性和亲水性强,不属于分泌蛋白且无跨膜结构域,有多个磷酸化和糖基化修饰位点,主要在细胞核内发挥作用,具有2个保守性结构域,与DNAJC5、HSPB1、DNAJA1和GAK等蛋白存在相互作用;HSAP8基因在雏鸡各组织中广泛表达,其中在胰腺中表达量最高,在胸肌中表达量最低;应激模型评价结果发现,断喙应激和热应激导致鸡血清皮质酮、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)等含量显著增加(P<0.05),T细胞亚群(CD3^(+))和免疫球蛋白G(IgG)含量有所降低;断喙应激模型鸡胸腺中IL-1β、IL-6、TNF-α和IL-8基因表达量在第1天显著升高(P<0.05),第5天显著降低(P<0.05);热应激模型鸡胸腺中IL-1β、IL-6、TNF-α和IL-8基因表达量显著升高(P<0.05);HSPA8基因在断喙后第1和3天胸腺中表达量显著升高(P<0.05),第5天显著降低(P<0.05);在持续热应激组中显著降低(P<0.05)。[结论]HSPA8基因参与鸡胸腺应激性免疫应答反应,可作为反映家禽是否处于应激性免疫抑制状态的可靠指标。以上结果为进一步研究HSPA8的免疫调控功能提供参考依据。展开更多
车辆检测是智能交通系统和自动驾驶的重要组成部分。然而,实际交通场景中存在许多不确定因素,导致车辆检测模型的准确率低实时性差。为了解决这个问题,提出了一种快速准确的车辆检测算法——YOLOv8-DEL。使用DGCST(dynamic group convol...车辆检测是智能交通系统和自动驾驶的重要组成部分。然而,实际交通场景中存在许多不确定因素,导致车辆检测模型的准确率低实时性差。为了解决这个问题,提出了一种快速准确的车辆检测算法——YOLOv8-DEL。使用DGCST(dynamic group convolution shuffle transformer)模块代替C2f模块来重构主干网络,以增强特征提取能力并使网络更轻量;添加的P2检测层能使模型更敏锐地定位和检测小目标,同时采用Efficient RepGFPN进行多尺度特征融合,以丰富特征信息并提高模型的特征表达能力;通过结合GroupNorm和共享卷积的优点,设计了一种轻量型共享卷积检测头,在保持精度的前提下,有效减少参数量并提升检测速度。与YOLOv8相比,提出的YOLOv8-DEL在BDD100K数据集和KITTI数据集上,mAP@0.5分别提高了4.8个百分点和1.2个百分点,具有实时检测速度(208.6 FPS和216.4 FPS),在检测精度和速度方面实现了更有利的折中。展开更多
基金the Talent Management Project of Prince of Songkla University
文摘Wearable sensing systems have been designed to monitor health conditions in real-time by detecting analytes in human biofluids.Wound diagnosis remains challenging,necessitating suitable materials for high-performance wearable sensors to offer prompt feedback.Existing devices have limitations in measuring pH and the concentration of pH-dependent electroactive species simultaneously,which is crucial for obtaining a comprehensive understanding of wound status and optimizing biosensors.Therefore,improving materials and analysis system accuracy is essential.This article introduces the first example of a flexible array capable of detecting pyocyanin,a bacterial virulence factor,while correcting dynamic pH fluctuations.We demonstrate that this combined sensor enhances accuracy by mitigating the impact of pH variability on pyocyanin sensor response.Customized screen-printable inks were developed to enhance analytical performance.The analytical performances of two sensitive sensor systems(i.e.,fully-printed porous graphene/multiwalled carbon nanotube(CNT)and polyaniline/CNT composites for pyocyanin and pH sensors)are evaluated.Partial least square regression is employed to analyze nonzero-order data arrays from square wave voltammetric and potentiometric measurements of pyocyanin and pH sensors to establish a predictive model for pyocyanin concentration in complex fluids.This sensitive and effective strategy shows potential for personalized applications due to its affordability,ease of use,and ability to adjust for dynamic pH changes.
文摘[目的]探讨鸡热休克蛋白8(heat shock protein family A member 8,HSPA8)的生物学特性及其在应激状态下胸腺中的表达模式。[方法]试验以固始鸡胸腺为材料,利用PCR技术扩增并克隆HSPA8基因CDS区,通过生物信息学方法分析其生物学特性和结构特点,并检测其在不同组织中的相对表达量。构建断喙应激和热应激模型,采用实时荧光定量PCR技术检测炎症因子和HSPA8基因在不同应激模型鸡胸腺中的表达情况。[结果]鸡HSPA8基因CDS区长度为1 941 bp,共编码氨基酸646个,与红原鸡基因序列相似性为98.92%;HSPA8基因核苷酸序列的系统发育树结果表明,固始鸡与火鸡亲缘关系最近,与哺乳动物次之,与低等鱼类亲缘关系最远;HSPA8蛋白属于酸性蛋白,稳定性和亲水性强,不属于分泌蛋白且无跨膜结构域,有多个磷酸化和糖基化修饰位点,主要在细胞核内发挥作用,具有2个保守性结构域,与DNAJC5、HSPB1、DNAJA1和GAK等蛋白存在相互作用;HSAP8基因在雏鸡各组织中广泛表达,其中在胰腺中表达量最高,在胸肌中表达量最低;应激模型评价结果发现,断喙应激和热应激导致鸡血清皮质酮、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)等含量显著增加(P<0.05),T细胞亚群(CD3^(+))和免疫球蛋白G(IgG)含量有所降低;断喙应激模型鸡胸腺中IL-1β、IL-6、TNF-α和IL-8基因表达量在第1天显著升高(P<0.05),第5天显著降低(P<0.05);热应激模型鸡胸腺中IL-1β、IL-6、TNF-α和IL-8基因表达量显著升高(P<0.05);HSPA8基因在断喙后第1和3天胸腺中表达量显著升高(P<0.05),第5天显著降低(P<0.05);在持续热应激组中显著降低(P<0.05)。[结论]HSPA8基因参与鸡胸腺应激性免疫应答反应,可作为反映家禽是否处于应激性免疫抑制状态的可靠指标。以上结果为进一步研究HSPA8的免疫调控功能提供参考依据。
