Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from t...Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from two kind of Chinese medicinable herbs were tested. Human melanocytes were obtained from neonatal foreskins and 48-well culture dish covered with fibronectin were used to perform melanocyte adhesion assay; Motility was assessed using the micropore filter method. Results: The extracts of Bu Gu Zhi(Psoralea corylifolia L), Bai Zhi(Dahurian angelica root) obviously showed an effect in increasing of human melanocyte adhesion and migration on fibronectin. Conclusion It is suggested that Buguzhi(Fructus Psoraleae) and Baizhi(Radix Angelicae Dahuricae) might induce melanocyte adhesion and/or migration in the treatment of vitiligo.展开更多
目的 探讨补骨生髓方对去势骨质疏松大鼠Smad/ERK信号通路的影响。 方法 通过采取去性腺法(摘除睾丸法)建立骨质疏松症大鼠实验模型,造模成功后按照实验方案对大鼠进行灌胃治疗,2个月后处死各组大鼠,HE常规染色,并通过免疫组织化学染色...目的 探讨补骨生髓方对去势骨质疏松大鼠Smad/ERK信号通路的影响。 方法 通过采取去性腺法(摘除睾丸法)建立骨质疏松症大鼠实验模型,造模成功后按照实验方案对大鼠进行灌胃治疗,2个月后处死各组大鼠,HE常规染色,并通过免疫组织化学染色的方法检测正常对照组及各中药干预组去势大鼠ERK、p-ERK、Smad4蛋白的表达。 结果 与模型组比较,补骨生髓方高剂量组与中剂量组ERK蛋白的表达差异有统计学意义 ( P <0.05), 而低剂量组与模型组表达差异无统计学意义 ( P >0.05);与模型组比较,补骨生髓方高剂量组与中剂量组p-ERK蛋白的表达差异有统计学意义( P <0.05),而低剂量组与模型组表达差异无统计学意义( P >0.05);与模型组比较,补骨生髓方高剂量组与中剂量组Smad4蛋白表达差异有统计学意义( P <0.05),而低剂量组与模型组比较差异无统计学意义( P >0.05)。 结论 补骨生髓方在一定程度上能够上调ERK、p-ERK、Smad4蛋白的表达水平,其可能是通过调控ERK蛋白磷酸化介导的Smad/ERK信号通路来发挥作用;ERK、Smad4可能是今后治疗骨质疏松的潜在作用靶点,但仍需要进一步开展更深入的研究。展开更多
文摘Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from two kind of Chinese medicinable herbs were tested. Human melanocytes were obtained from neonatal foreskins and 48-well culture dish covered with fibronectin were used to perform melanocyte adhesion assay; Motility was assessed using the micropore filter method. Results: The extracts of Bu Gu Zhi(Psoralea corylifolia L), Bai Zhi(Dahurian angelica root) obviously showed an effect in increasing of human melanocyte adhesion and migration on fibronectin. Conclusion It is suggested that Buguzhi(Fructus Psoraleae) and Baizhi(Radix Angelicae Dahuricae) might induce melanocyte adhesion and/or migration in the treatment of vitiligo.
文摘目的 探讨补骨生髓方对去势骨质疏松大鼠Smad/ERK信号通路的影响。 方法 通过采取去性腺法(摘除睾丸法)建立骨质疏松症大鼠实验模型,造模成功后按照实验方案对大鼠进行灌胃治疗,2个月后处死各组大鼠,HE常规染色,并通过免疫组织化学染色的方法检测正常对照组及各中药干预组去势大鼠ERK、p-ERK、Smad4蛋白的表达。 结果 与模型组比较,补骨生髓方高剂量组与中剂量组ERK蛋白的表达差异有统计学意义 ( P <0.05), 而低剂量组与模型组表达差异无统计学意义 ( P >0.05);与模型组比较,补骨生髓方高剂量组与中剂量组p-ERK蛋白的表达差异有统计学意义( P <0.05),而低剂量组与模型组表达差异无统计学意义( P >0.05);与模型组比较,补骨生髓方高剂量组与中剂量组Smad4蛋白表达差异有统计学意义( P <0.05),而低剂量组与模型组比较差异无统计学意义( P >0.05)。 结论 补骨生髓方在一定程度上能够上调ERK、p-ERK、Smad4蛋白的表达水平,其可能是通过调控ERK蛋白磷酸化介导的Smad/ERK信号通路来发挥作用;ERK、Smad4可能是今后治疗骨质疏松的潜在作用靶点,但仍需要进一步开展更深入的研究。
