Sequential experimental observation on the curative effect of Yingbupu decoction of Zhuang medicine on stage I and II acute kidney injury

Objective:To observe the clinical efficacy of the Zhuang medicine Yingbupu decoction on stage I and II acute kidney injury through sequential test.Methods:The open one-way qualitative response sequential design of experiments was adopted,and the patients with AKI in phase I and II who met the inclusion criteria were divided into the treatment group and the control group according to the order of hospitalization by random number table.On the basis of basic treatment,the treatment group was treated with Zhuang medicine Yingbupu decoction,and the control group was treated with Jinshuibao tablet.The clinical efficacy,TCM syndrome score,24 h urine volume,serum creatinine(Scr),microalbumin in urine(mAlb),neutrophil Gelatinase related lipid delivery albumin(NGAL)of the two groups were compared,and the adverse reactions and complications of the two groups were observed.Results:After 14 d of treatment,when the treatment group reached the 10th case,the experimental line contacted the upper bound U-line and reached the experimental standard to terminate the experiment.The effective hypothesis was accepted,and it was believed that the Zhuang medicine Yingbupu decoction had a therapeutic effect on stage I and II AKI.The conclusion was drawn that the treatment group received the Zhuang medicine Yingbupu.The clinical effective rate and improvement days were similar between the two groups,and there was no significant difference(P>0.05).However,the integral value of traditional Chinese medicine syndrome in the treatment group was lower than that in the control group(P<0.05),After treatment,the Scr,mAlb,and NGAL levels of patients in both groups were lower than before treatment(P<0.05).After treatment,the Scr,mAlb,and NGAL values in the treatment group were significantly lower than those in the control group(P<0.05).After treatment,the 24-hour urine volume in both groups was higher than that before treatment,and the values in the treatment group were significantly higher than those in the control group(P<0.05).During the treatment period,there were no significant adverse reactions or complications in either group.Conclusion:The Zhuang medicine Yingbupu decoction is effective in treating stage I and II AKI,and the Zhuang medicine Yingbupu can significantly improve the symptoms and quality of life of patients with stage I and II AKI.Its improvement of renal function is better than that of Jinshuibao tablets,and its safety is good.

Transcriptomic and metabolomic analysis of the effects of Zhenwu decoction on kidney yang deficiency pattern in chronic kidney disease

Objective:To explore the kidney yang deficiency pattern(KYDP)in a chronic kidney disease(CKD)rat model and the mechanisms underlying the effects of Zhenwu decoction(ZWD)by conducting tran-scriptomic and metabolomic analyses.Methods:Adriamycin(ADR)combined with hydrocortisone(HC)was used to induce CKD with KYDP in rats.ADR was injected into the tail vein twice.HC was injected intramuscularly for 8 weeks.ZWD was administered by gavage for 8 weeks.The general condition was observed,24-h urine protein was detected,serum corticosterone,triiodothyronine,thyroxine,TSH,testosterone,cAMP,and cGMP levels were determined,and pathological analysis was conducted.Transcriptomic and metabolomic analyses were conducted to screen differentially expressed genes(DEGs),differentially expressed metabolites(DEMs),and differentially expressed pathways(DEPs).The core DEMs and DEGs were input to Metab-oanalyst 5.0 to identify the pathways affected by ZWD.Results:In the HC group,KYDP symptoms were observed.Compared with control group,the levels of 24-h urine protein,TSH,and cGMP significantly increased(all P<0.01),and corticosterone,triiodothyronine,thyroxine,and cAMP significantly decreased(all P<0.01)in the HC group.After ZWD intervention,the levels of above-mentioned indicators could be reversed to some extent.Pathological analysis in the HC group revealed kidney lesions.DEGs in the ZWD group were mainly associated with pathways such as nucleotide synthesis and endocrine pathways.In the ZWD group,differences in biosynthesis of unsat-urated fatty acids and butanoate metabolism were observed.The following pathways were significantly affected by ZWD:arachidonic acid metabolism,valine,leucine,and isoleucine biosynthesis,linoleic acid metabolism,and alpha-linolenic acid metabolism.Conclusion:ZWD can be used to treat KYDP in CKD through regulating arachidonic acid metabolism,valine,leucine,and isoleucine biosynthesis,linoleic acid metabolism,and alpha-linolenic acid metabolism.

Efficacy and safety of the decoction of nourishing marrow and raising energy in terms of Traditional Chinese Medicine in patients with vascular dementia:a randomized controlled trial

OBJECTIVE: To explore the efficacy and safety of the decoction of nourishing marrow and raising energy in terms of Traditional Chinese Medicine(TCM) in patients with vascular dementia.METHODS: Totally 86 patients with vascular dementia patients hospitalized from September 2010 to January 2015 were recruited and randomly divided into two groups: control group and observation group. Patients in control group received the conventional treatment, whereas patients in another group were treated with the therapy of nourishing marrow and raising energy according to the theory of TCM. Blesse dementia scale scores were measured before and after the treatment for both groups and adverse effect was documented as well.RESULTS: After treatment, Blesse dementia scale scores reduced in both groups, and the difference in score reduction between the two groups was significant(P<0.05). The rate of adverse events was significantly lower in observation group than in control group(P<0.05).CONCLUSION: Our results demonstrate that the decoction of nourishing marrow and raising energy was an effective and safe alternative to vascular dementia in human subjects.

Art of Nourishing Life According to the Kidney Meridian in Zhenjiu Dacheng(The Great Compendium of Acupuncture and Moxibustion)

“Daoyin Benjing”(Art of Nourishing Life)discusses the kidney meridian in depth.It is a dense and heterogeneous text that is heavily influenced by Daoism.The text proposes rules for the maintenance of life using language that is often cryptic and under-standable only to the initiated.This language is closely interwoven with the classical terms of traditional Chinese medicine.The kidney is the home of vital energy,which is a type of sexual energy and the root of life.Engagement in moderate sexual activity nourishes life and leads to a long life.This is the goal of every Daoist follower and the goal of every doctor.

Effects of Gancao Nourish-Yin Decoction on Liver Metabolic Profiles in hTNF-αTransgenic Arthritic Model Mice

Objective Gancao Nourish-Yin Decoction(GNYD)has been applied to clinical rheumatoid arthritis(RA)patients,and it had shown effectiveness not only in disease activity controlling but also in improving patients'physical status.However,its mechanism of function has not been investigated.Metabolic perturbations have been associated with RA,and targeting the metabolic profile is one of the ways to manage the disease.The aim of this study is to observe the effect of GNYD on metabolic changes of human tumor necrosis factorα(hTNF-α)transgenic arthritic model mice.Methods hTNF-αtransgenic arthritic model mice were divided into the control group and the GNYD group with six mice in each group.After 8 weeks of treatment,liver tissues of mice in both groups were obtained for liquid chromatography-mass spectrometry analysis.Significantly regulated metabolites by GNYD treatment were first identified,followed by Kyoto Encyclopedia of Genes and Genomes pathway and network analysis.Results A total of 126 metabolites were detected in the liver.Compared with the control group,17 metabolites in the GNYD group were significantly altered.Specifically,thiamine,gamma-L-glutamyl-L-valine,pantothenic acid,pyridoxal(vitamin B6),succinic acid,uridine 5′-diphospho-glucuronic acid,uridine,allantoic acid,N-acetyl-D-glucosamine,nicotinamide ribotide,and N2,N2-dimethylguanosine were down-regulated by GNYD treatment,whereas isobutyrylglycine,N-acetylcadaverine,N-carbamoyl-L-aspartic acid,L-anserine,creatinine,and cis-4-hydroxy-D-proline were up-regulated.Six metabolic pathways were significantly altered including the alanine,aspartate,and glutamate metabolism;pyrimidine metabolism;thiamine metabolism;amino sugar and nucleotide sugar metabolism;pantothenate and CoA biosynthesis;and citrate cycle.Integrative metabolic network analysis suggested the possibility of GNYD having both positive and negative effects on RA through the suppression of angiogenesis and the promotion of leukocyte extravasation into the synovium,respectively.Conclusions GNYD can modulate the hepatic metabolism of hTNF-αtransgenic arthritic model mice.Further optimization of this decoction may lead to better therapeutic effects on RA patients.

Clinical observation of kidney-qi deficiency syndrome patients with hypertension by Bushenjiangya decoction

OBJECTIVE: To observe the clinical efficacy of Bushenjiangya(reinforcing kidney and lowering blood pressure) decoction in curing hypertension of kidney Qi deficiency type. METHODS: First 60 patients with hypertension of kidney Qi deficiency type were divided into control group and treatment group randomly; the conventional western medicine was given to patients of the control group for antihypertensive therapy, while the traditional Chinese medicine Bushenjiangya decoction was added for patients in the treatment group based on the therapy for those in control group; after 2 months, the blood pressure, clinical efficacy and urine microalbumin changes of patients in the two groups before and after the treatment were observed. RESULTS: a. The systolic pressure of patients in the two groups was all reduced obviously(P<0.05); b. The total effective rate of experiment group in antihypertensive effect was 76.7%, while that of control group was 83.3%(P>0.05); c. The total effective rate of experiment group in TCM syndrome effect was 86.7%, while that of control group was 63.3%, showing that the effect of experiment group was better than that of control group(P<0.05); d. The 24 h urine microalbumin reduction of experiment group was better than that of control group(P<0.05). CONCLUSIONS: The curative effect of Bushenjiangya decoction in combination with the western medicine is better than that of simple western medicine for hypertension of kidney Qi deficiency type, and also the urine microalbumin can be obviously reduced to protect the kidney function.

滋肾养心安神方治疗心肾不交型失眠的疗效及对过度觉醒状态的影响

目的 观察滋肾养心安神方治疗心肾不交型失眠的疗效及对过度觉醒状态的影响。方法 选择2023年4—11月上海市第五人民医院中医科诊治的心肾不交型失眠患者94例,按随机数字表法分为治疗组和对照组,每组47例。对照组给予艾司唑仑片口服4周;治疗组于第1~2周给予艾司唑仑片联合滋肾养心安神方治疗,第3~4周仅给予滋肾养心安神方治疗。观察比较2组治疗前后匹兹堡睡眠质量指数(PSQI)评分、中医证候评分、过度觉醒量表(HAS)评分、生活质量量表(SF-36)评分,统计2组治疗4周后失眠疗效、中医证候疗效。结果 2组治疗后PSQI各项评分及总分、HAS评分均较治疗前明显降低(P均<0.05),且治疗组各项评分均明显低于对照组(P均<0.05)。2组治疗后中医证候中不寐、出汗、心悸、五心烦热、头晕、健忘评分及总评分和治疗组治疗后腰酸、咽干少津、月经不调/梦遗阳痿评分均较治疗前明显降低(P均<0.05),且治疗组不寐、出汗、心悸、五心烦热、头晕、腰酸、健忘、咽干少津评分及总评分均明显低于对照组(P均<0.05)。2组治疗后SF-36总分及各项评分均较治疗前明显升高(P均<0.05),且治疗组治疗后SF-36总分及一般健康状况、健康变化、生理功能、生理职能、情感职能、社会功能、精力、精神健康评分均明显高于对照组(P均<0.05)。治疗组失眠总有效率为89.36%(42/47),中医证候总有效率为97.87%(46/47),对照组分别为72.34%(34/47)和8.51%(4/47),治疗组失眠和中医证候总有效率均明显高于对照组(P均<0.05)。结论 滋肾养心安神方治疗心肾不交型失眠疗效显著,可明显改善患者睡眠质量和过度觉醒状态,减轻中医症状,提高生活质量。

李宜瑞辨治注意缺陷多动障碍经验

注意缺陷多动障碍是儿童时期常见的以注意力不集中、多动和冲动为特征的精神-心理-行为障碍疾病之一。李宜瑞教授认为,本病系脏腑阴阳失调所致,心脾肾不足乃病机之本,常兼肝亢、痰湿等标证。临证时宜审慎辨证,用药以四君子汤为基础方,重视健脾,合用龙骨、首乌藤、白芍、石菖蒲、远志、益智、合欢皮、连翘等以增智养神、祛痰开窍,心脾肾同治;同时根据伴随症状进行加减用药,标本兼顾,以调和脏腑阴阳,从而改善注意缺陷多动障碍患儿的注意力、记忆力。

基于气阴两虚夹瘀运用五参汤治疗肺癌术后发热浅析

肺癌术后发热有非感染性发热与感染性发热之分,在气虚、阴虚、瘀血阻滞病理状态下形成热蕴状态,是为非感染性发热;而术中感染或外感风寒入里化热则形成感染性发热表现。其基本病机均可概括为气阴两虚夹瘀。五参汤具有益气养阴、清热解毒之功,为证属正气亏虚、外感邪毒者而设,用于肺癌术后发热契合其病机。

名中医陈锋从“肝主筋、为罢极之本”论治腰椎间盘突出症经验

腰椎间盘突出症主要由椎间盘各部分(包括髓核、纤维环及软骨板)出现不同程度的退行性改变,受外力作用的因素下,椎间盘的纤维环破裂,髓核组织从破裂处突出或脱出于后方或椎管内,刺激或压迫相邻脊神经根,产生腰部疼痛,一侧或双侧下肢麻木、疼痛等一系列临床症状。中医学通常把本病归纳在“腰痛”范畴,病因病机以肾虚为本,兼有外感风寒湿热或瘀血邪阻。陈锋教授认为肝脏亦为本病的核心病机之一,提出从“肝主筋,为罢极之本”溯发病之源,以肝不束筋骨致椎间盘退变为始、肝疏泄失司经不络属致腰背筋脉失常为标、肝筋劳损过度罢极不复致腰部筋力涣散为变的核心病机,日久腰背部气血失调、筋骨失衡发为本病。临证善于抓主症、辨主因、守病机,专病专药随症加减化裁,采用补肝养血、行气祛瘀为遣方思路,同时中药内服与外敷结合,注重手法推拿、腰背肌功能锻炼,发挥中医中药的临床价值。

益气补肾止喘汤结合中医推拿对哮喘患儿PET、C-ACT评分的影响

目的:观察益气补肾止喘汤结合中医推拿对哮喘患儿正电子发射计算机断层显像(positron emission tomography PET)、儿童哮喘控制测试(children-asthma control test, C-ACT)评分的影响。方法:选取太原市中医医院2021年1月至2022年12月收治的哮喘患儿104例,均符合小儿哮喘临床诊断标准,随机分成对照组和研究组,每组52例。对照组给予常规西药治疗,研究组给予益气补肾止喘汤结合中医推拿治疗。观察两组患儿治疗前后肺功能相关指标[1秒用力呼气容积(forced expiratory volume in one second, FEV1)、用力肺活量(forced vital capacity, FVC)与FEV1/FVC]、正电子发射计算机断层显像(positron emission tomography, PET)、C-ACT评分及临床症状消失时间、临床疗效。结果:研究组治疗后FEV1、FVC、FEV1/FVC高于对照组,差异有统计学意义(P<0.05);对照组有效率为67.31%,研究组有效率为96.15%,两组有效率比较,差异具有统计学意义(P<0.05);研究组临床症状消失时间短于对照组,差异具有统计学意义(P<0.05);研究组治疗后PET、C-ACT评分高于对照组,差异具有统计学意义(P<0.05)。结论:益气补肾止喘汤结合中医推拿能提高哮喘患儿肺功能,改善其临床症状。

健脾养正消癥方对脾胃虚弱型中晚期胃癌化疗患者肠道菌群的影响

目的基于肠道微生态学探讨健脾养正消癥方辅助胃癌化疗可能的机制。方法将2021年1—12月于江苏省中医院住院治疗的46例脾胃虚弱型中晚期胃癌患者随机分为对照组23例和试验组23例,另以同期健康志愿者30例作为健康组。对照组患者给予以奥沙利铂联合氟尿嘧啶类为基础的化疗方案治疗,试验组患者在化疗(方案同对照组)基础上给予健脾养正消癥方加减口服,2组均完成2个化疗周期治疗。比较2组患者治疗前后KPS评分、中医证候积分及治疗后胃肠道不良反应发生情况;采集2组患者治疗前后和30例健康人的粪便样本,利用16SrDNA测序技术分析肠道菌群物种结构、相对丰度。结果试验组患者治疗后KPS评分明显高于治疗前及对照组(P均<0.05),中医证候积分明显低于治疗前及对照组(P均<0.05),对照组患者治疗前后KPS评分及中医证候积分比较差异均无统计学意义(P均>0.05)。试验组患者治疗后腹泻的发生率明显低于对照组(P<0.05)。3组Shannon指数比较差异均无统计学意义(P均>0.05)。2组患者治疗前后Chao指数均明显高于健康组(P均<0.05),但试验组患者治疗后Chao指数明显低于对照组(P<0.05),试验组患者治疗后菌群物种结构较对照组更接近健康组。2组患者治疗前梭杆菌门、脱硫弧菌属、梭杆菌属及颤螺旋菌科UCG_002属的相对丰度均明显高于健康组(P均<0.05),霍氏真杆菌属明显低于健康组(P均<0.05)。对照组患者治疗后霍氏真杆菌属相对丰度明显下降(P<0.05),颤螺旋菌科UCG_002属相对丰度明显上升(P<0.05);试验组患者治疗后霍氏真杆菌属相对丰度明显上升(P<0.05),梭杆菌门、梭杆菌属相对丰度明显下降(P均<0.05),颤螺旋菌科UCG_002属相对丰度治疗前后比较差异无统计学意义(P>0.05)。结论健脾养正消癥方可能通过调节肠道菌群的丰富度,逆转化疗导致的部分肠道菌群变化,增加有益菌,减少条件致病菌,从而发挥辅助脾胃虚弱型中晚期胃癌化疗的作用。

根据“肾藏精”理论及老年不寐病机从肾虚精亏论治老年不寐

我国患有睡眠障碍的老年人约占40%~70%,睡眠障碍已经成为严重困扰老年人生活质量的关键因素。改善老年失眠可以大大提高老年人生活质量。基于“肾藏精”理论并结合老年人生理病理特点,老年人先天之精、后天之精、肾阴、肾阳亏虚均能致不寐。老年不寐的病机,为“以虚为本”,即肾精亏虚是根,痰饮瘀血互结、虚实并见,易变生他病,常兼杂心肝火旺及脾虚。吾师陈民临床常用补肾填精之方引火汤,配以清肝健脾之药治疗老年不寐。并附典型案例1则。

柴胡桂枝干姜汤病机新解与临床应用

柴胡桂枝干姜汤出自《伤寒杂病论》,历代医家对其病机认识各有不同,导师袁红霞从原文入手,条文互参,参考《神农本草经》和《黄帝内经》等,以经解经,得出柴胡桂枝干姜汤的病机有以下两点:一是表邪仍在,邪热内陷;二是阳虚为主兼以阴伤。据此将柴胡桂枝干姜汤功效拆分为祛邪、扶阳、滋阴三部分,并根据疾病所处阶段不同予以加减应用。如欲以祛邪为主,若见表邪盛则增加柴胡、黄芩用量以解表祛邪,若见邪热内陷明显则炙甘草改生甘草以增强清热之功,同时可减少桂枝用量以达到宣通气机之用;如欲以扶阳为主,则增加干姜用量,严重时致肾阳虚损可配伍附子理中丸;若欲以滋阴为主,则减干姜用量,增加栝楼根、牡蛎用量以增强滋阴清热生津之力。附病案1则。

朱涛如经验方新制龙牡汤探析及临床应用

探讨芜湖市名老中医朱涛如经验方新制龙牡汤的组方用意和临床应用。类比新制龙牡汤与经典名方柴胡加龙骨牡蛎汤、桂枝加龙骨牡蛎汤的异同:三方虽均有安神定志之用意,但与柴胡加龙骨牡蛎汤相比,新制龙牡汤针对的是无形虚邪而非有形实邪;与桂枝加龙骨牡蛎汤相比,新制龙牡汤更偏于滋阴而非温阳。新制龙牡汤具有育阴潜阳、调和营卫等功效。本文总结朱老临床应用该方治疗高血压眩晕、梦遗滑精、肾盂肾炎等疾病的经验,类比其他医家相似医案治法治意的异曲同工之处。另附笔者临床应用新制龙牡汤加减治疗帕金森综合征医案一则。

附子理中汤“补火生土”治疗溃疡性结肠炎机制探讨

溃疡性结肠炎(ulcerative colitis,UC)是以腹泻腹痛、黏液脓血便为主要特征的慢性肠炎,发病率呈逐年上升趋势,病变可累及结肠、直肠等部位,临床上具有难治愈、病程长、易复发等特点。临床中附子理中汤常联合西药、中医外治法等治疗脾肾阳虚型UC,现有大量实验研究和临床实践证实附子理中汤治疗脾肾阳虚型UC具有独特的优势。附子理中汤通过“补火生土”补肾阳滋脾土之阳实现“温补命门以培土,暖脾固肠而止泻”,以脾肾双补、温阳驱寒来治疗脾肾阳虚之证。且有相关实验及临床研究印证附子理中汤主要从抗炎镇痛、维持肠道免疫平衡、修复肠黏膜、调节肠道菌群、缓解氧化应激等方面治疗UC。该文将对附子理中汤“补火生土”治疗脾肾阳虚型UC的中医理论基础、作用机制以及临床研究进行系统论述,旨在为研究附子理中汤治疗UC提供进一步的理论依据以及实践参考。

彭剑虹运用育阴潜阳法治疗原发性干燥综合征经验介绍

介绍彭剑虹教授运用育阴潜阳法治疗原发性干燥综合征的临床经验。原发性干燥综合征属中医燥痹范畴。彭剑虹教授认为,燥痹并非单纯的津液亏乏,其发病存在阴精的不足、阴虚阳亢的特点。临证参何炎燊名老中医运用育阴潜阳法治疗阴虚阳亢疾病之核心思想,运用育阴潜阳法治疗燥病,常用方为三甲复脉汤,并在此方的基础上加减滋补肝肾、益气健脾、活血通络之药。

滋阴潜阳汤配合耳穴按压对高血压患者左心室舒张功能的影响

目的:观察滋阴潜阳汤配合耳穴按压对高血压患者左心室舒张功能的影响。方法:将90例高血压患者根据随机数字表法分为常规组和研究组,每组各45例。常规组给予耳穴按压治疗,研究组在常规组治疗的基础上联合滋阴潜阳汤治疗。监测两组患者治疗前后24 h血压变化情况,比较两组患者不良反应发生率及治疗前后主动脉内径(aorta,AO)、左心室射血分数(left ventricular ejection fraction,LVEF)、E峰、A峰、E/A、凝血因子Ⅷ、抗凝血酶Ⅲ、组织型纤溶酶原激活物(tissue-type plasminogenactivator,t-PA)等指标变化情况。结果:两组患者治疗后各项血压指标低于本组治疗前,且治疗后研究组低于常规组(P<0.05)。两组患者治疗后AO、LVEF、E峰、A峰及E/A高于本组治疗前,且治疗后研究组高于常规组(P<0.05)。两组患者治疗后凝血因子Ⅷ、抗凝血酶Ⅲ及t-PA等各项凝血纤溶系统指标高于本组治疗前,且治疗后研究组高于常规组(P<0.05)。研究组不良反应发生率低于常规组(P<0.05)。结论:滋阴潜阳汤配合耳穴按压治疗高血压疗效确切,可降低患者血压水平,改善凝血纤溶系统指标及左心室舒张功能,且不良反应较少。

国医大师沈宝藩应用养心汤治疗失眠临床经验分享

失眠为临床常见病。长期失眠,严重影响患者的身心健康和生活质量。国医大师沈宝藩从医60余年,擅治内科疾病。对于治疗失眠,积累了丰富的经验。沈宝藩教授认为失眠主要病机为心肝血虚,心神不宁。治疗上以养血安神为法,创立了养心汤,意在养血安神、调肝养心,临床收效颇佳,附验案两则加以阐明。

基于PI3K/Akt/NF-κB信号通路探讨滋阴补肾方对糖尿病合并去卵巢骨质疏松大鼠骨代谢的影响

目的基于磷脂酰肌醇-3-激酶/蛋白激酶B/核因子κB(PI3K/Akt/NF-κB)信号通路探究滋阴补肾方对糖尿病合并去卵巢骨质疏松大鼠骨代谢的影响。方法将60只SPF级雌性大鼠随机分为对照组、模型组、骨化三醇组及滋阴补肾方高、中、低剂量组,每组10只。除对照组外,其余组大鼠均摘除双侧卵巢、喂食高糖高脂饲料并注射链脲佐菌素建立糖尿病合并去卵巢骨质疏松模型。造模成功后,滋阴补肾方高、中、低剂量组分别按照干药剂量20 g/kg、10 g/kg、5 g/kg灌胃滋阴补肾方药液,骨化三醇组灌胃0.1μg/kg骨化三醇,对照组和模型组灌胃等量生理盐水,均1次/d,连续8周。末次灌胃结束后,股动脉取血,自动生化分析仪测定空腹血糖(FPG)、钙、磷、三酰甘油(TG)、胆固醇(TC)水平;腹主动脉取血,ELISA法测定糖化血红蛋白(HbA1c)、空腹胰岛素(FINS)水平,计算胰岛素抵抗指数(HOMA-IR);RT-PCR法检测骨组织中PI3K、Akt、NF-κB mRNA相对表达量。结果与对照组比较,模型组FPG、HbA1c、HOMA-IR、TG、TC水平及骨组织中NF-κB mRNA相对表达量均明显升高(P均<0.05),FINS、血钙、血磷水平及骨组织中PI3K mRNA、Akt mRNA相对表达量均明显降低(P均<0.05);与模型组比较,骨化三醇组和滋阴补肾方各组FPG、HbA1c、HOMA-IR、TG、TC水平及骨组织中NF-κB mRNA相对表达量均明显降低(P均<0.05),FINS、血钙、血磷水平及骨组织中PI3K mRNA、Akt mRNA相对表达量均明显升高(P均<0.05),且滋阴补肾方高剂量组各指标改善情况均明显优于滋阴补肾方低剂量组(P均<0.05)。结论滋阴补肾方可通过介导PI3K/Akt/NF-κB信号通路改善糖尿病合并去卵巢骨质疏松大鼠的骨代谢,同时可调节糖代谢,减轻胰岛素抵抗。