Mandarin fish von Hippel-Lindau protein regulates the NF-κB signaling pathway via interaction with IκB to promote fish ranavirus replication

The von Hippel-Lindau tumor suppressor protein(VHL),an E3 ubiquitin ligase,functions as a critical regulator of the oxygen-sensing pathway for targeting hypoxia-inducible factors.Recent evidence suggests that mammalian VHL may also be critical to the NF-κB signaling pathway,although the specific molecular mechanisms remain unclear.Herein,the roles of mandarin fish(Siniperca chuatsi)VHL(scVHL)in the NF-κB signaling pathway and mandarin fish ranavirus(MRV)replication were explored.The transcription of scVHL was induced by immune stimulation and MRV infection,indicating a potential role in innate immunity.Dual-luciferase reporter gene assays and reverse transcription quantitative PCR(RT-qPCR)results demonstrated that scVHL evoked and positively regulated the NF-κB signaling pathway.Treatment with NF-κB signaling pathway inhibitors indicated that the role of scVHL may be mediated through scIKKα,scIKKβ,scIκBα,or scp65.Co-immunoprecipitation(Co-IP)analysis identified scIκBαas a novel target protein of scVHL.Moreover,scVHL targeted scIκBαto catalyze the formation of K63-linked polyubiquitin chains to activate the NF-κB signaling pathway.Following MRV infection,NF-κB signaling remained activated,which,in turn,promoted MRV replication.These findings suggest that scVHL not only positively regulates NF-κB but also significantly enhances MRV replication.This study reveals a novel function of scVHL in NF-κB signaling and viral infection in fish.

A Content Analysis Study Describing Information Regarding Female Genital Mutilation Present in US Medical Textbooks

This study examines the depth of information regarding Female Genital Mutilation (FGM)/Female Cutting (FC) present in US medical textbooks. According to Klein et al. (2018) [1], despite substantial attention regarding the effects of FGM, this practice is still ongoing all over the world. Healthcare providers are very important actors who need to understand FGM in order to provide care to girls and women subjected to it, but the small amount of existing work in this area shows a low level of knowledge and training about FGM/FC among U.S. physicians. The Center for Disease Control and Prevention (CDC) first published the estimated number of girls at risk of FGM/FC in the U.S. to be about 168,000 in 1997;that number had increased to 513,000 girls in the US by 2012 (according to Goldberg et al.) In this study, a small sample of medical textbooks were analyzed for the degree to which the textbooks include information regarding FGM/FC. The results of this study show that US medical textbooks do not include information regarding FGM/FC, suggesting that most medical providers don’t have knowledge and training about FGM/FC. The inadequate information on FGM/FC in medical textbooks reflects a culturally biased lack of recognition of the number of girls and women affected in the US. This maintains an ongoing pattern of silence that reinforces gender inequality. Considering the inadequate information covered in US medical textbooks on FGM/FC, and the concordant lack of provider training to provide advice and care to affected girls and women, incorrect and missed diagnoses may lead to harm to victims of FGM/FC. Providers would be better equipped to care for women and girls if their medical training included information regarding FGM/FC.

The Islamic Religion in Course Curriculums of High School History Textbooks in the Republic of Macedonia

History textbooks can and primarily should contribute to the strengthening of students’awareness of universal human values.However,history textbooks can also deepen the divisions,prejudices,and uncritical attitudes,contrary to the goals of education.The youth population,which is still building and creating their point of view towards the world is the main group in the education process.The learning period,in the elementary,as well as in the secondary education is of utmost importance for the development of every human.To that end,history,through the contents of the past largely shapes our perceptions of every individual,ourselves,and the others.Since we are living in a multiethnic and multi-confessional society,I have decided to conduct an analysis of the teaching contents of high school textbooks related to religion.Another reason for the analysis is the role of religion in the formation of Balkan nations,which have made it one of the key elements of their national identities.It is often given the role of raising and strengthening the national awareness,patriotism,as well as the country homogeneity,which most often leads to forming prejudices,divisions,and discrimination towards people of minority religions.The large volume of content related to the religion,and also the mere fact that Muslims are the second largest religion group in the population,I have decided to analyze the contents which are related only to the Islamic religion.The goal is to look for both the positive and negative sides of those contents in history textbooks in secondary schools,which are backed by state institutions that are supposed to take care of the development of competitive,creative,progressive,and ethically formed human potential.The critical review will give an additional impulse to the efforts of eliminating those parts of the teaching contents that create divisions and prejudices,especially in history textbooks,so that its studying can become the key factor in the building of trust between people of different religions.

Exploring Phonetic Differences and Cross-Linguistic Influences: A Comparative Study of English and Mandarin Chinese Pronunciation Patterns

Exploring the differences in phonemes and cross-linguistic influences, particularly by comparing the pronunciation patterns of English and Mandarin, is crucial for language learners. Such comparative studies can help learners better understand and overcome the pronunciation difficulties encountered during second language acquisition. English and Mandarin have significant differences in their vowel and consonant systems, tones, intonation, and syllable structures. A deep understanding of these differences allows learners to engage in targeted pronunciation training, reducing accent interference. This research provides guidance for improving language teaching methods, enabling teachers to design more effective pronunciation strategies and exercises based on the students’ native language backgrounds, thus enhancing learning outcomes. Additionally, cross-linguistic studies aid in improving speech recognition and conversion technologies, achieving higher accuracy and naturalness in multilingual speech processing systems. From a cultural exchange perspective, understanding and respecting the phonetic characteristics of different languages help to enhance the effectiveness and mutual understanding in cross-cultural communication. The comparative study of English and Mandarin pronunciation patterns not only provides a theoretical foundation for language education and technological applications but also promotes effective communication in multilingual environments. This thesis uses a comparative research method to elucidate the study of English and Mandarin pronunciation patterns. It begins with an analysis of the characteristics and similarities of the pronunciation patterns in both languages. The thesis then examines the differences between English and Mandarin pronunciation patterns through four aspects: the number and complexity of vowel phonemes, types and distribution of consonant phonemes, tones and stress, and intonation and phonetic phenomena. Finally, based on the comparative analysis of the differences, the thesis offers targeted policy recommendations for learning English and Mandarin.

Exploration and Research on Textbook Quality Evaluation Index System Based on Engineering Major in Higher Education

The report of the Chinese 20th National Congress put forward“strengthening the construction and management of textbooks”for the first time,which indicates the importance of textbook construction and highlights the vital position of textbooks in the overall development of the country.From the perspective of textbook quality,and based on the research and analysis of the policy requirements and current evaluation indicators of higher education textbooks,this paper establishes the textbook quality evaluation index system composed of basic indicators and evaluation indicators.This system is based on the principles of science,comprehensiveness,operability,and target-oriented approach.It provides a relatively scientific,objective,and fair reference system for the management and evaluation of textbooks.

The Existing Problems and Optimization Paths of Digital Textbooks Under Background of Internet

With the rapid development of information technology,the combination of terminal technology,big data and mobile Internet and textbooks has become an irresistible trend in the modern education field.Under the context of the Internet,carrying out education and teaching activities based on digital textbooks can give full play to the rich media,openness and interaction of digital textbooks,broaden students′horizon,enrich students′knowledge,and promote the improvement of students′ability and all-round development.However,in the specific teaching practice,there are also problems such as old compilation ideas,single compilation mode and low efficiency of personalized learning.Therefore,schools and teachers need to constantly innovate the presentation and arrangement of digital textbooks,strengthen technical support,deepen students′understanding of the teaching content of digital textbooks,promote the comprehensive development of students and improve the effectiveness of digital textbook teaching.

Issues and Countermeasures in Chinese FLTRP Primary School Textbooks under the New Curriculum Standard

The New Curriculum Standard(NCS)is the norm and quality requirement of the Chinese national educational curriculum as well as the basis of teaching management,curriculum evaluation,and textbook compilation.In textbook design,the selection of textbook materials should meet the objectives and requirements of the NCS.Since appropriate teaching materials are essential to curriculum standards and effective teaching,we must follow the consistency between textbook materials and curriculum standards to achieve an ideal degree of conformity with the textbook system and teaching objectives and promote the sustainable development of the English curriculum reform.However,the current primary school English textbooks fall short of the NCS,which leads to difficulties in implementing teaching tasks and achieving teaching goals.Given these difficulties,we should analyze the causes from three dimensions:linguistic knowledge,cultural awareness,and learning strategies.In addition,a high recurrence of phonetic knowledge,systematic grammar presentation,diversified learning styles,level grading,and in-depth involvement of cultural factors are keys to the perfect conformity of the NCS and English textbooks.

Measurement Analysis and Evaluation of Twenty- Five Years of Chinese Mathematics Textbooks: Visual Analysis Based on CiteSpace Knowledge Graph (1999-2024)

At present,textbooks based on core literacy have become the inevitable demands of China’s curriculum reform,and the literacy of textbook goal construction is the key to the implementation of core literacy requirements,which is a huge challenge for textbook compilers.In this paper,we use the visual metrology of the CiteSpace knowledge graph to analyze Chinese mathematics textbooks(1999-2024),hoping to guide the future direction of Chinese mathematics textbook research.

Knowledge Graph Analysis of International Chinese Language Textbooks Based on CiteSpace

Drawing upon relevant papers from Chinese core journals and CSSCI source journals in the CNKI China Academic Journals Full-Text Database spanning from 1992 to 2023,this study utilizes CiteSpace as a research tool to visually analyze the knowledge graph structure of research on international Chinese language textbooks in China.The study maps out the publication timeline,authors,institutions,collaborative networks,and keywords pertaining to research on international Chinese language textbooks.The findings indicate that research on international Chinese language textbooks commenced early and continues to maintain a certain level of research interest,yet lacks sufficient research output.Research institutions predominantly reside in universities and publishing groups specializing in language or education,with collaboration between institutions being relatively scarce.High-frequency keywords in recent research on international Chinese language textbooks include“Chinese language textbooks for the Foreigners,”“Chinese language textbooks,”“Teaching Chinese Language for the Foreigners,”“Textbook compilation,”“International Chinese Language Education and Localization,”which reflect a diversified research perspective with interdisciplinary trends.Future research priorities encompass research on localization,customization of textbooks,and evaluation of textbooks which represent forefront directions of research.

Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi

Carnivorous fish have poor tolerance to carbohydrate in feed and low utilization rate of carbohydrate.Therefore,the balance of carbohydrate and lipids in the nutrient metabolism of carnivorous fish,the ef fective conversion and utilization of carbohydrate and lipids,and the feedback regulation of feeding are the key links for the e fficient utilization of carnivorous fish feed.Carbohydrate response element binding protein(ChREBP)is a new transcription factor found in recent years in the glucose signaling pathway,and can also participate in feeding regulation.We performed in-vivo and in-vitro experiments to reveal the role of ChREBP in the glucose metabolism and feeding in mandarin fish.The mRNA expression of ChREBP and appetite regulatory factors were measured after intraperitoneal injection of glucose in mandarin fish Siniperca chuatsi and cotransfection with glucose and glucose+siRNA in the hypothalamic cells in mandarin fish.The results reveal that at hour 2 and 4 post intraperitoneal injection with 1 mg/g BW glucose,the blood glucose level of the mandarin fish increased significantly,but food intake decreased significantly,and it also displayed a significantly increased ChREBP mRNA expression levels in liver.At hour 4 post injection,hypothalamic ChREBP mRNA level was significantly increased,whereas the mRNA expression levels of appetite genes neuropeptide Y(npy)and agouti-related peptide(AgRP)were decreased significantly.When the glucose concentration was 40 mmol/L,the expression level of ChREBP mRNA in mandarin fish hypothalamic cells was significantly up-regulated,but the expression level of appetite gene npy mRNA was down-regulated.When siRNA and glucose were co-transfected into mandarin fish brain cells,the expression level of chrebp mRNA was significantly decreased,and the appetite gene npy mRNA was significantly increased.The results indicated that glucose regulated food intake through the modulation of appetite gene npy by ChREBP.

Analysis of textbook outcomes for ampullary carcinoma patients following pancreaticoduodenectomy

BACKGROUND Textbook outcomes(TOs)have been used to assess the quality of surgical treatment for many digestive tumours but not ampullary carcinoma(AC).AIM To discuss the factors associated with achieving a TO and further explore the prognostic value of a TO for AC patients undergoing curative pancreaticoduodenectomy(PD).METHODS Patients who underwent PD at the China National Cancer Center between 1998 and 2020 were identified.A TO was defined by R0 resection,examination of≥12 Lymph nodes,no prolonged hospitalization,no intensive care unit treatment,no postoperative complications,and no 30-day readmission or mortality.Cox regression analysis was used to identify the prognostic value of a TO for overall survival(OS)and recurrence-free survival(RFS).Logistic regression was used to identify predictors of a TO.The rate of a TO and of each indicator were compared in patients who underwent surgery before and after 2010.RESULTS Ultimately,only 24.3%of 272 AC patients achieved a TO.A TO was independently associated with improved OS[hazard ratio(HR):0.443,95%confidence interval(95%CI):0.276-0.711,P=0.001]and RFS(HR:0.379,95%CI:0.228-0.629,P<0.001)in the Cox regression analysis.Factors independently associated with a TO included a year of surgery between 2010 and 2020(OR:4.549,95%CI:2.064-10.028,P<0.001)and N1 stage disease(OR:2.251,95%CI:1.023-4.954,P=0.044).In addition,the TO rate was significantly higher in patients who underwent surgery after 2010(P<0.001)than in those who underwent surgery before 2010.CONCLUSION Only approximately a quarter(24.3%)of AC patients achieved a TO following PD.A TO was independently related to favourable oncological outcomes in AC and should be considered as an outcome measure for the quality of surgery.Further multicentre research is warranted to better elucidate its impact.

中国特色教育学教材体系:价值意蕴、逻辑依循与建构路径

教育学教材是对教育学学科知识的系统阐述,是教育学学科体系、学术体系和话语体系的核心载体。加快构建中国特色教育学教材体系既是学科发展之需,也是时代发展之要。中国特色教育学教材体系建设有其自身内在的逻辑取向,具体表现为坚持马克思主义指导地位,奠定思想根基和根本保证是政治逻辑;凸显育人为本,落实立德树人根本任务是价值逻辑;以文化自信为精神硬核,承担培根铸魂重任是文化逻辑;运用系统观念,推动体系内外诸多环节的有机融合是方法逻辑;遵循守正创新,为教育学教材体系建设注入源头活水是实践逻辑。在着力推进中国教育学“三大体系”建设的进程中,教育学教材体系的建构路径主要包括秉守正确的政治方向和价值取向,明晰教育学教材体系建设目标;加强相关理论研究,筑牢教育学教材体系建设基础;强调中国自主知识体系建构,彰显教育学教材体系建设特色;完善教材建设制度,提升教育学教材体系建设质量。

The Grammatical Categorization of Mandarin在/zài:Spatiality,Temporality and Semantic Construal

Mandarin在(pinyin:zài)is the most frequently used character in representing spatial and temporal relationship.Current studies mostly focus on its lexical meaning and syntactic structure while cognitive features of its grammatical categories have been neglected.This paper investigates into the categorization of zài by conducting a morphosyntactic test among College English majors in China.The results show that:prototypes are organizing the grammatical categories of zài at all levels in terms of intra-categorial gradience;the semantic construal of zài construction could significantly influence the accuracy of the grammatical categorization of zài;the syntactic structure can provide viable cue for the identification of grammatical categories of zài;spatiality,temporality and the status of existing are three essential semantic features encoded by zài,the concurrence of which leads to various degree of inter-categorial vagueness,indicating a conflict between the rigid grammatical classification and the indeterminate nature of the grammatical functions of zai,suggesting the necessity to reconsider the efficacy of applying indiscriminately the Anglo-Saxon grammar into the study of Chinese spatial-temporal constructions.

Investigation on the Integration of “Gao” in Hong Kong Chinese and Mainland Mandarin

From the diachronic dimension,this paper makes a two-way interactive investigation on the integration of Hong Kong Chinese and Mainland Mandarin.“Gao”is a delexical verb in contemporary Chinese.In different words and expressions related to“gao”character,there are mutual absorptions between Chinese mainland and Hong Kong,which is characterized by starting from nothing to something and from less to more.

职业教育新形态教材的主要特征、实践审视与开发策略

“教材形态”是教材作为一种教学中介的外在形态与内在形态的表征形式,教材形态变化引领着课程教学的变革,进而影响着教育的高质量发展。职业教育新形态教材具有内容选择的职业情境性、编写体例的工作过程性、知识与技能更新的及时性、学习内容的丰富性和学习环境的可交互性等特征。职业教育新形态教材开发实践中,存在教材定位偏离起点、教材质量参差不齐、开发团队水平不高等问题。职业教育新形态教材的开发策略为:回归培养“完整人”的逻辑起点;完善教材开发团队的运行机制;践行工作过程系统化编写体例;导入学习情境化模块单元。

新时代高质量数字化教材建设的价值意蕴、基本逻辑和实践路径

新时代高质量数字化教材建设是国家教材体系现代化的应有之义,需要探析其育人价值、人本价值与技术价值,为数字化教材建设提供方向指引。新时代高质量数字化教材建设要坚持以学生“善学”为出发点的目标逻辑、以个性化教学为着眼点的内容逻辑、以技术赋能为着力点的形态逻辑。构建新时代高质量数字化教材就要做到:把握数字化教材建设的时代方位,即包括坚持中国共产党的领导、注重数字素养的培养及贯彻“数字中国”战略;擘画数字化教材建设的未来图景,形成数字化教材与纸质教材互构的内容体系、数字化教材与个性化发展需求互动的服务体系、数字化教材与技术互融的应用体系;完善保障机制,明确数字化教材建设主体间权责关系,健全规章体系,创新管理机制。

“理解当代中国”翻译类教材使用研究——以《汉法翻译教程》为例

培养符合时代需求的高水平翻译人才始终是翻译教育领域的核心课题。提升翻译人才培养质量的一个重要途径在于加强教材建设。“理解当代中国”翻译类教材定位明确、理念新颖、设计科学,为翻译教学提供了可靠依据和有力支撑。如何科学使用该系列教材,最大程度地发挥其育人作用,无疑是当下翻译人才培养中应着力探究的问题。本文以《汉法翻译教程》为例,从注重价值引领、把握正确的翻译原则以及注重翻译能力提升、实施分析—探究—互动三位一体式教学等方面对“理解当代中国”翻译类教材的使用展开深入探讨,以期为新时代翻译人才培养提供些许有益的参考。

以多元能力为导向的大学英语教材编写实践研究

作为教学内容的主要载体和教学必备工具,大学英语教材直接影响教师如何组织课堂、实施教学,学生加工什么样的输入、进行什么样的产出、培养什么样的能力。为满足新时代培养国际化人才的需求,大学英语教学确立了多元能力培养目标,这些目标应通过教材编写落实在具体的教学内容和活动设计上,从而引导教学实践真正服务于能力培养。本研究以《新未来大学英语》教材为例,探讨以多元能力为导向的大学英语教材的编写,提炼核心原则与策略,以期为教材开发与教学设计提供参考。

职业教育国家规划教材内容特征分析——以财经商贸大类为例

内容是教材的灵魂。采用内容分析法,从教材设计理念、教材结构、教材设计、纸数融合四个维度,对财经商贸大类“十四五”职业教育国家规划教材内容特征进行分析。研究发现:认可能力本位教材设计理念,与标准对接不足;“章—节”式目录结构仍占较大比重,教材内容主题分歧明显;标题式项目、理实叠加现象突出;数字资源以二维码融入微课视频为主,存在不一致、使用率不高问题。据此,提出提升高职专业教学标准适应性、内容描述详尽度和时效性,重视专业核心课程教材的技术理论知识开发,以实践性问题为纽带整合理论与实践知识,结合课程内容特性与学习者需求开发教材配套的数字化资源等优化对策。

The Role and Positioning of Digital Textbooks in Vocational Education in Relation to Teachers

In recent years,with the rapid advancement of digital technologies,digitization in vocational education has become a major trend.Among these advancements,digital textbooks have emerged at the forefront of digitization,significantly impacting both their form and content.This article aims to analyze the existing issues in the use of digital textbooks in vocational education and emphasize the importance of recognizing the vital role of teachers in the wave of digital textbook innovation.It is crucial to clarify the role and positioning of teachers to avoid inadvertently diminishing their classroom significance while prioritizing technological innovations.Teachers should fully leverage the role of digital textbooks as tools for organizing classroom activities and recognize the core and essence of classroom teaching as digital technologies enter the scope of vocational education.By doing so,teachers can contribute to the high-quality development of modern vocational education.