基于病程相宜理论的康复训练对老年慢性心力衰竭患者心功能、6 min步行距离及护理效果的影响

目的 探究基于病程相宜理论的康复训练实施效果对老年慢性心力衰竭患者心功能水平、6 min步行距离构成的影响。方法 选取2022年10月至2023年10月本本院收治的慢性心力衰竭患者72例,采用随机数字表法分为对照组(n=36)、观察组(n=36),前者实施常规护理,后者所用护理模式为基于病程相宜理论的康复训练,对比干预效果,以心功能水平、6 min步行距离、生活质量为评估指标。结果 干预前,两组心功能水平对比,差异无统计学意义(P>0.05);干预后,观察组心功能水平优于对照组,差异有统计学意义(P<0.05),观察组6 min步行距离远于对照组,差异有统计学意义(P<0.05),观察组生活质量各项指标数值高于对照组,差异有统计学意义(P<0.05)。结论 老年慢性心衰患者采用基于病程相宜理论的康复训练对于提高自身运动耐力以及促进心功能水平恢复具有突出效果,有利于提升整体生活质量。

阿托伐他汀诱导的MIN6细胞铁死亡及相关机制研究

目的:探讨阿托伐他汀(atorvastatin,Ator)是否可诱导小鼠胰岛β细胞株MIN6细胞发生铁死亡,并探讨其可能的作用机制。方法:将MIN6细胞分为对照组、Ator组、Ator+凋亡抑制剂(Z-VAD-FMK)组、Ator+坏死抑制剂(necrostatin-1,Nec-1)组和Ator+铁死亡抑制剂(ferrostatin-1,Fer-1)组。采用CCK-8法检测细胞活力;透射电镜观察细胞超微结构;荧光显微镜观察活性氧(reactive oxygen species,ROS)和Fe^(2+)水平;酶联免疫吸附试验(enzyme-linked immuno sorbent assay,ELISA)检测丙二醛(malondialdehyde,MDA)和还原型谷胱甘肽(glutathione,GSH)含量;实时荧光定量PCR法(quantitative real-time PCR,RT-qPCR)检测凋亡基因半胱氨酸蛋白酶3(caspase-3)、坏死基因受体结合丝氨酸苏氨酸激酶3(receptor-interacting serine threonine kinase 3,Ripk3)、铁死亡相关基因长链酯酰辅酶A合成酶4(acyl-coA synthetase long-chain family member 4,Acsl4)、前列腺素内过氧化物合酶2(prostaglandin-endoperoxide synthase 2,Ptgs2)和谷胱甘肽过氧化物酶4(glutathione peroxidase 4,Gpx4)的mRNA表达水平;Western blot检测4-羟基壬烯醛(4-hydroxynonenal,4-HNE)和GPX4的蛋白表达水平。结果:与Ator组相比,Ator+Z-VAD-FMK组和Ator+Fer-1组细胞存活率更高(P均<0.01)。透射电镜下Ator组细胞可见凋亡、铁死亡和自噬相关的形态学特征。与对照组相比,Ator组细胞Fe^(2+)相对荧光强度、MDA水平和ROS相对水平均升高,GSH含量下降;caspase-3、Acsl4、Ptgs2的mRNA及4-HNE的蛋白表达增加(P均<0.05),GPX4的mRNA和蛋白表达减少(P<0.05)。与Ator组相比,Ator+Fer-1组Fe^(2+)相对荧光强度、MDA水平和ROS相对水平均下降,GSH含量上升;Acsl4的mRNA表达减少,Gpx4的mRNA表达增加(P均<0.05);4-HNE的蛋白表达减少而GPX4的蛋白表达增加,但差异无统计学意义。结论:Ator可能通过抑制甲羟戊酸途径下调GPX4表达,诱导MIN6细胞发生铁死亡。

慢性心力衰竭病人6 min步行试验与代谢当量的相关性

目的:探讨慢性心力衰竭(CHF)病人6 min步行距离(6MWD)与代谢当量(MET)的相关性。方法:纳入符合射血分数降低标准的CHF病人60例,进行心肺运动试验(CPET)与2次6 min步行试验(6MWT)。采集2次6MWD、峰值摄氧量(VO_(2)peak)、无氧阈下代谢当量(METs-AT)、血氧饱和度(SpO_(2))、心率等数据,采用线性回归分析建立6MWD与METs-AT之间的回归方程模型。结果:正式6MWT中6MWD、VO_(2)peak、METs均较预行6MWT显著提高,分别增加5.02%、7.83%及8.61%,差异有统计学意义(P<0.05),2次6MWT过程中,病人的心率、Borg评分、SpO_(2)变化趋势基本一致;正式6MWD与METs-AT相关存在线性关系(r=0.78),方程式中决定系数R2和常量均具有统计学差异(P<0.05);试验过程中未出现严重不良事件。结论:正式6MWT结果可作为评估运动耐量的准确测量指标,根据6MWT建立回归模型推导METs-AT适用于CHF病人的评估。

细菌分裂调控Min系统的功能、机制及应用研究进展

细菌分裂过程及其调控机制是生物学研究中的根本问题。大多数细菌采用“二分裂”方式分裂,分裂过程主要包括染色体复制和分离、FtsZ组装成Z环、细胞内隔膜形成与子细胞形成等关键步骤。Min系统是Z环组装的主要调控系统之一,由FtsZ抑制蛋白MinC、膜结合的ATP酶MinD和结构拓扑因子MinE组成。早期研究大多聚焦在Min系统的细胞分裂调控功能,然而Min系统还作为细胞内部重要的自组装调控系统参与多种细胞代谢生理过程与细胞表型,具有应用开发潜力。本文首先简要概述Min系统的主要类型、结构特征和摆动模型,随后从细胞分裂、细胞运动性、细菌粘附性与致病性、细胞代谢生理过程等方面总结Min系统的生物学功能,最后介绍Min系统在细胞形态工程、抗菌制剂开发和合成生物学应用等方面的应用潜力。

具有多级适应性休假和Min(N,V)-策略的Geo=G=1排队系统分析

本文研究具有多级适应性休假和Min(N,V)-策略的Geo/G/1排队系统,运用更新过程理论,全概率分解技术和z-变换工具,分析在时刻点n^(+)的队长瞬态概率分布的z-变换表达式.此外,基于瞬态分析,使用洛必达法则得到系统在时刻点n+的队长稳态分布的递推表达式,然后给出稳态队长分布的概率母函数以及平均稳态队长的表达式.接着,探讨在不同时刻点n^(-)、n和外部观察点的稳态概率分布.最后,在建立费用结构模型下,由更新报酬过程理论获得系统长期运行单位时间内所产生的成本期望费用的表达式,并通过数值实例讨论最优控制策略N^(*)以及当连续休假次数为正整数J时的二维最优控制策略(N^(*),J^(*)).

Linguistic landscape and its impacts on place-making in mountain destination:A case of Mogan Mountain Town of Huzhou City,China

The place and placeness are believed to be a crucial perspective for comprehending the transformative dynamics of tourist destinations in the context of globalization and the rapid development of the tourism industry.As the material carrier of language,linguistic landscape is an intermediary for people to endow emotions and personalities into the place,becoming an effective path for exploring the place-making in a certain area.Mogan Mountain is a famous national tourist resort,which is located in Mogan Mountain Town,Deqing County,Huzhou City,China.This is a good case area for studying the relationship between linguistic landscape and placemaking in tourist destinations.There are two theories that form the theoretical foundation of the study,namely the place and the geosemiotics.Firstly,the results showed the overall style of the linguistic landscape.Multilingual labelling(52.7%),official signs(55.3%),and standardized Chinese characters predominantly shaped the linguistic landscape,with English and other languages being complementary.Secondly,from the perspective of three dimensions of the geosemiotics framework,such as inscription,language preference,and placement position,we conduct a comprehensive semiotic analysis of the linguistic landscape,which encompasses diverse facets such as fonts,materials,shifts in state,text vectors,and symmetrical arrangements.Finally,we explore the influence of linguistic landscapes on the place-making of Mogan Mountain through the dimensions of location,locale,and sense of place.The information on linguistic landscapes indicates the geographical location.These linguistic landscapes play an important role in constructing the language order,nostalgic atmosphere,exotic atmosphere,and elegant and tasteful lifestyle of the public space in Mogan Mountain.Readers of linguistic landscapes,including tourists and residents,generate and identify with the unique sense of place in Mogan Mountain.These linguistic landscapes construct the destination placeness of Mogan Mountain that combines both foreign and nostalgic styles,and the integration of Chinese and Western cultures.It will help promote the image positioning of tourism destinations and have practical guiding significance for the tourism planning,landscape-making,language management,and other aspects of tourism destinations.

Dynamically enhanced Autler–Townes splitting by orthogonal XUV fields

Based on numerical solutions of the time-dependent Schr ¨odinger equation, we theoretically investigate the photoelectron spectrum of hydrogen atoms ionized by a pair of ultrashort, intense, and orthogonally polarized laser pulses with a relative time delay in a pump–probe configuration. The pump pulse resonantly excites electrons from the 1s and 2p levels,inducing Rabi oscillations. The resulting dynamically enhanced Autler–Townes(AT) splitting is observed in the photoelectron energy spectrum upon interaction with the second probe pulse. In contrast to the previous parallel-polarization scheme, the proposed orthogonal-polarization configuration enables the resolution of dynamically enhanced AT splitting over a considerably wider range of probe photon energies.

Mito-TEMPO Ameliorates Sodium Palmitate Induced Ferroptosis in MIN6 Cells through PINK1/Parkin-Mediated Mitophagy

Objective Mitochondrial reactive oxygen species(mtROS)could cause damage to pancreaticβ-cells,rendering them susceptible to oxidative damage.Hence,investigating the potential of the mitochondriatargeted antioxidant(Mito-TEMPO)to protect pancreaticβ-cells from ferroptosis by mitigating lipid peroxidation becomes crucial.Methods MIN6 cells were cultured in vitro with 100μmol/L sodium palmitate(SP)to simulate diabetes.FerroOrange was utilized for the detection of Fe2+fluorescence staining,BODIPY581/591C11 for lipid reactive oxygen species,and MitoSox-Red for mtROS.Alterations in mitophagy levels were assessed through the co-localization of lysosomal and mitochondrial fluorescence.Western blotting was employed to quantify protein levels of Acsl4,GPX4,FSP1,FE,PINK1,Parkin,TOMM20,P62,and LC3.Subsequently,interventions were implemented using Mito-TEMPO and Carbonyl cyanide 3-chlorophenylhydrazone(CCCP)to observe changes in ferroptosis and mitophagy within MIN6 cells.Results We found that SP induced a dose-dependent increase in Fe2+and lipid ROS in MIN6 cells while decreasing the expression levels of GPX4 and FSP1 proteins.Through bioinformatics analysis,it has been uncovered that mitophagy assumes a crucial role within the ferroptosis pathway associated with diabetes.Additionally,SP decreased the expression of mitophagy-related proteins PINK1 and Parkin,leading to mtROS overproduction.Conversely,Mito-TEMPO effectively eliminated mtROS while activating the mitophagy pathways involving PINK1 and Parkin,thereby reducing the occurrence of ferroptosis in MIN6 cells.CCCP also demonstrated efficacy in reducing ferroptosis in MIN6 cells.Conclusion In summary,Mito-TEMPO proved effective in attenuating mtROS production and initiating mitophagy pathways mediated by PINK1 and Parkin in MIN6 cells.Consequently,this decreased iron overload and lipid peroxidation,ultimately safeguarding the cells from ferroptosis.

Science and Technology Commissioners Supporting High-Quality Development Project for Hundreds of Counties, Thousands of Towns, and Myriads of Villages in the Context of Rural Revitalization

This paper discusses the important role of science and technology commissioners in the high-quality development of hundreds of counties,thousands of towns,and myriads of villages in the context of rural revitalization,including building bridges,accelerating the transformation of achievements,promoting the value-added of the whole agricultural industry chain,and promoting the rapid development of rural industrial economy.It also discusses the working achievements of science and technology commissioners,in order to promote further development of rural revitalization in Guangdong Province.

Huangyao Ancient Town

HUANGYAO Ancient Town is located in the northeast of Zhaoping County in Hezhou City,south China’s Guangxi Zhuang Autonomous Region.Around 60 kilometers from downtown,the ancient town covers an area of over 3.2 square kilometers.It traces its origin to the early days of the Song Dynasty(960-1279).The town expanded during the Ming Dynasty(1368-1644)and flourished during the Qing Dynasty(1644-1911).

Allocation of Public Service Facilities in Villages and Towns from the Perspective of Smart Contraction: A Case Study of Zixi County, Jiangxi Province

The limitation of traditional layout system and the continuous contraction of villages and towns have brought challenges to the layout of public service facilities.It is an imperative task against this background to integrate space resources and allocate public service facilities scientifically and reasonably.This paper took the system and method of rural public service facility allocation from the perspective of smart contraction as the theoretic support,and Zixi County in Jiangxi Province for example,built the four-level life circle of“county-fundamental-basic-routine”based on the influence factors of facility allocation,village and town contraction strength,rural comprehensive assessment results,residents’trip willingness and demands on public service facilities,finally proposed the suggestions for optimizing the allocation of village and town public service facilities.

基于Max-min distance聚类算法的园地空间聚类--以永泰县嵩口镇为例

空间聚类是空间数据挖掘的重要手段之一。本文研究了一种基于质心点距离的Max-min distance空间聚类算法:通过加载园地图斑数据,计算其园地图斑质心,判断聚类中心之间的距离,并将符合条件的园地图斑进行聚类,最终将聚类结果可视化表达。本文的算法是利用Visual Studio 2017实验平台和ArcGIS Engine组件式开发环境,采用C#语言进行编写。实验结果表明:1)Max-mindistance聚类通过启发式的选择簇中心,克服了K-means选择簇中心过于邻近的缺点,能够适应嵩口镇等山区丘陵地区空间分布呈破碎的园地数据集分布,有效地实现园地的合理聚类;2)根据连片面积将园地空间聚类结果分为大中小三类,未来嵩口镇可以重点发展园地连片规模较大的村庄,形成规模化的青梅种植园。

Landscape Architecture Planning and Design Based on Landscape Ecology Theory:A Case Study of Waterfront Landscape Planning and Design of Dapo Town

With the rapid development of urbanization,landscape architecture has become an indispensable component of daily life.In order to achieve better landscape effects and protect the ecological environment,landscape ecology theory can be integrated into landscape planning to carry out disciplinary crossing and integration research between landscape ecology and landscape architecture.In this study,based on the basic theories of landscape ecology,the waterfront landscape of Dapo Town was designed,with the aim to achieve ecological balance in planning,showcase biodiversity,and create a harmonious landscape ecological environment which can benefit both nature and people.This design combined artificial and natural elements while improving the natural environment,which promoted a harmonious relationship between humans and nature,thus enriching the spatial organization of the city,and playing a role in regulating the urban environment and climate.

Spatial Protection and Development of Traditional Mountain Settlements from the Perspective of Self-organization : A Case Study of Anju Ancient Town in Tongliang District, Chongqing

Through literature research, field research and urban design analysis methods, based on the self-organization theory, the evolution characteristics of the settlement space of Anju Ancient Town with significant characteristics of traditional mountain settlements were analyzed, and then its internal and external influencing factors were summarized. Finally, appropriate protection and development strategies were proposed, such as reasonably planning functional zones, breaking through the single structure, and creating a good living cultural environment. The innovation of this study is that it integrates the self-organization theory into the cognition of traditional mountain settlement space, and discusses the connection between the mountain settlement space and the traditional regional context under the modern development concept, so as to seek the benign protection and reasonable development of traditional mountain settlement space.

城市不同区位15 min生活圈便利度对比研究——以广州市某区为例

15 min生活圈是人类城市生活中最基础的生活范围,除了15 min步行范围,15 min内的公交和自驾区域也成为当下居民日常的活动范围。本研究基于路网计算了城市不同区位15 min步行、公交和自驾3种类型的生活圈,并利用POI获取公服设施的空间分布情况,对生活圈的便利度进行探究。研究发现,研究区中,所有公服设施都呈现出东北西南向的空间分布,且向市区聚集;研究区内15 min步行生活圈的便利度高于自驾和公交,城区的便利度高于城郊和郊区,但郊区和城郊的便利度差异不大。

My Good Friend at Hometown

I have a good friend.His name is Zhang Qiang.He is from Shiyan City,my hometown.He is a middle school student,too.He is very tall and strong.He can play basketball very well.Singing and dancing are his favorite hobbies.I like playing ping-pong.So he often plays ping-pong with me in P.E. class.

Study on the Impacts of Tourism Development in Letong Ancient Town:A Resident Perception-Based Approach

Residents constitute the principal elements of regional tourism deveopment,and their behavioral cognition will play a certain role in promoting local tourism development.In recent years,although tourism along the Sichuan-Tibet Line(318 and 317 routes)has made some progress,there are some problems,such as homogenization of products,a single local culture,and inconspicuous brand characteristics.At the same time,due to the remote geographical location,living conditions,health conditions,and other imperfections,resulting in greater constraints on tourism development.This article employs Letong Ancient Town as a representative case study,and discusses the impact of tourism development on the local economy,environment,society,and culture from the perspective of residents’perception through field research and data analysis,thereby providing a reference for regional tourism development.

Development Strategies for Z County E-sports Town in Chongqing:Integration of E-sports,Tourism,and Agriculture under New Policies

Chongqing Z County E-sports Town was once included in the“first batch of sports and leisure characteristic town pilot project list”issued by the General Administration of Sports of China.After several years of development,the e-sports industry in Z County e-sports town has struggled with issues such as a limited market,low popularity,poor business performance,and a lack of income generation capabilities.These challenges have severely hindered the town’s development,resulting in its exclusion from the recent“Chongqing characteristic town list.”Under the requirements of new policies,it can no longer promote itself,attract investment,or undertake project construction under the name of a“characteristic town”and must undergo redevelopment.This paper employs case analysis and comparative investigation methods to conduct an in-depth empirical study on the development of Z County E-sports Town.It is proposed that under the new policy environment,the town should foster the deep integration of its existing e-sports industry and culture with tourism and agriculture,transforming Z County E-sports Town into a new characteristic tourist destination.The findings of this paper offer a reference for the development of similar towns facing stagnation and provide case support for the construction of tourist destinations featuring e-sports.

慢性心力衰竭患者接受沙库巴曲缬沙坦治疗对6min步行距离的影响分析

分析慢性心力衰竭患者采用沙库巴曲缬沙坦开展治疗,对其6min步行距离等产生的影响。方法 2022年1月到2024年1月时间段内,于本院接受治疗的慢性心力衰竭患者一共有106例,采用信封法对患者进行均等分组,对照组治疗方案采用缬沙坦用药,研究组治疗方案则以对照组为基础加用沙库巴曲缬沙坦,对比组间的6min步行距离及相关指标等差异。结果 与对照组进行比较,研究组治疗的总有效率更高(P<0.05);研究组6min步行距离明显更长(P<0.05);研究组心功能相关指标均显示更优P<0.05);研究组出现的不良反应发生率与对照组差异并不是很明显(P>0.05)。结论 慢性心力衰竭患者接受沙库巴曲缬沙坦用药,患者的疾病治疗总有效率有明显提升,运动耐力、心功能显著增强,患者的6min步行距离更长,且不良反应结果统计显示未明显增加,整体治疗效果都表现得更理想。

彩色多普勒超声联合血清NSE及5 min Apgar评分对新生儿颅内出血的诊断价值及危险因素分析

目的探讨彩色多普勒超声联合血清神经元特异性烯醇化酶(neuron-specific enolase,NSE)及5 min Apgar评分对新生儿颅内出血(intracranial hemorrhage,ICH)的诊断价值及影响ICH发生的危险因素。方法选取2019年2月至2021年3月承德市中心医院新生儿科收治的存在颅脑损伤危险因素的253例新生儿为研究对象,均接受彩色多普勒超声检查,根据是否存在ICH分为ICH组(n=99)和无ICH组(n=154)。观察并比较两组彩色多普勒超声参数[收缩期峰值流速(peak systolicvelocity,PSV)、阻力指数(resistance index,RI)、舒张末期流速(end diastolic velocity,EDV)]、血清NSE水平、5 min Apgar评分情况,分析血清NSE水平、Apgar评分与彩色多普勒超声参数的相关性及三者联合检测对新生儿ICH的诊断价值,并分析ICH发生的主要影响因素。统计学方法采用独立样本t检验、χ^(2)检验、Pearson相关性分析、Logistic回归分析及受试者操作特征(receiver operating characteristic,ROC)曲线分析。结果ICH组与无ICH组PSV[(6.4±1.2)cm/s与(10.1±1.4)cm/s,t=21.628]、RI(0.6±0.1与0.7±0.1,t=8.144)、EDV[(2.5±0.4)cm/s与(3.1±0.4)cm/s,t=13.216]以及5 min Apgar评分[(6.5±1.7)分与(8.8±1.0)分,t=13.308]比较,ICH组均显著低于无ICH组(P值均<0.001);血清NSE水平显著高于无ICH组[(149.1±10.6)μg/L与(95.2±10.4)μg/L,t=40.015,P<0.001]。ICH组血清NSE水平与彩色多普勒超声参数PSV、RI、EDV呈负相关(r值分为-0.573、-0.520、-0.536,P值均<0.05);5 min Apgar评分与彩色多普勒超声参数PSV、RI、EDV呈正相关(r值分别为0.601、0.529、0.505,P值均<0.05)。ROC曲线结果发现,彩色多普勒超声、血清NSE水平、5 min Apgar评分联合诊断新生儿ICH的曲线下面积(area under the curve,AUC)最大,为0.861。单因素分析显示,与无ICH组比较,ICH组患儿的胎龄更小,出生体质量、5 min Apgar评分更低,出生窒息、应用多巴胺、应用机械通气比例及血清NSE水平更高,差异有统计学意义(P值均<0.05)。多因素Logistic回归分析结果显示,胎龄<32周、出生体质量<1500 g、血清NSE水平>117.95μg/L、5 min Apgar评分<7分是诱发ICH的独立危险因素。结论彩色多普勒超声联合血清NSE及5 min Apgar评分可提高ICH的诊断价值;胎龄<32周、出生体质量<1500 g、血清NSE水平>117.95μg/L、5 min Apgar评分<7分是诱发ICH的独立危险因素。