^(60)Co-γ射线与NaN_3复合处理对珍汕97A的诱变效应

以珍汕 97A为材料 ,通过不同剂量的 6 0 Co- γ射线和 Na N3药剂对珍汕 97A进行复合处理 ,分析研究它们的诱变效应。结果表明 ,随着辐射剂量的增加 ,M1群体中的育性恢复突变率有所下降 ;而分离出不育株的 M2 株系占育性恢复突变 M1株系的比例逐渐增加 ;与珍汕97A相比 ,M2 分离出的不育株有的仅发生农艺性状的变异 ,有的则在不育性的表达和农艺性状上均发生变异 ;与珍汕 97B相比 ,育性恢复的 M2 群体部分农艺性状发生了多种变异 ,并在M2 群体中出现了无花粉型不育株及弯秆突变体。此外 ,还讨论了诱变技术对不育系的改良与创新的有关问题。

不同基因型苹果的离体再生特性及其对NaN_3的反应

不同基因型苹果叶片培养物再生特性不同，主要表现在再生频率的高低和再生类型的差异。通过培养基中的激素和培养过程的光照调节，可以有效地提高各基因型品种的再生频率。不同基因型苹果叶片培养物对ＮａＮ３诱变处理的反应不同，总体而言，其存活率和再生频率均随ＮａＮ３浓度的提高而下降。不同基因型苹果的适宜ＮａＮ３浓度应有所不同，以３０％存活率为标准，对Ｍ２６、嗄拉、首红、长富２及岩富１０进行诱变处理的适宜浓度分别为３ｍｍｏｌ／Ｌ、３ｍｍｏｌ／Ｌ、３ｍｍｏｌ／Ｌ、２ｍｍｏｌ／Ｌ和２ｍｍｏｌ／Ｌ。

一种DNAN基熔铸炸药压滤装药工艺安全性分析

为确保混合炸药在压滤装药工艺过程中的安全性,通过工艺过程安全性分析,获得了工艺安全性风险因素及其分类;利用试验测试和数值仿真手段,对一种DNAN基熔铸混合炸药(MCX-D)的本质安全性、相容性、机械和热刺激等装药工艺安全性影响因素进行了分析与研究。结果表明,炸药本质上是安全的,压滤工艺过程中组分变化和热损伤导致炸药本身感度的升高有限(机械感度≤40%),符合使用要求;装药在温度和压力双重作用下的内部温升仅为0.3℃左右,不存在热爆炸的可能性。由此证明DNAN基熔铸炸药压滤装药工艺是安全可靠的。

NaN_3和紫外线复合诱变对苜蓿愈伤组织在干旱和盐胁迫下生长的影响

以图牧2号苜蓿愈伤组织为材料,对其进行不同浓度NaN_3及紫外线诱变剂量和不同浓度NaCl及PEG胁迫处理,观察愈伤组织生长状况,以愈伤组织的存活率确定各处理的半致死浓度(LC_(50))或剂量(LD_(50))和致死浓度(LC_(100))或剂量(LD100),并对愈伤组织进行脯氨酸、可溶性糖、丙二醛含量和CAT酶活性的测定。结果表明:诱变和胁迫影响了苜蓿愈伤组织生长,随NaN_3浓度和紫外线诱变剂量的增加,愈伤组织的存活率下降,NaN_3的LC_(50)和LC_(100)分别为4.0×10^(-3)mol·L^(-1)和5.5×10^(-3)mol·L^(-1),在距离为20cm时,紫外线诱变LD_(50)和LD_(100)分别为6、8min;随NaCl和PEG选择压力的加大,愈伤组织的存活率下降,NaCl的LC_(50)和LC_(100)分别为1.5%和2.0%,PEG的LC_(50)和LC_(100)分别为25%和30%。各诱变和胁迫处理比较,经NaN3和紫外线复合诱变及PEG和NaCl双重选择压力处理愈伤组织存活率最低为16%。NaN3和紫外线复合诱变的愈伤组织脯氨酸、可溶性糖含量、CAT酶活性增加,丙二醛含量下降。诱变和逆境胁迫筛选的苜蓿愈伤组织,可以提高苜蓿愈伤组织对盐旱的抵抗能力,可为利用体细胞扩大苜蓿抗逆性变异来源提供基础。

Olfactory Responses of Tomicus yunnanensis to Different Non-host Plants

[ Objective ] With Cupressustorulosa, Cinnamomum camphora and Cyclobalanopsis Oerst as test materials, the paper studied the effects of different non- host plants on olfactory responses of Tomicus yunnanensis. [ Method ] The needles of Pinus yunnanensis were mixed with the leaves of Cupressustondosa, C. cam- phora and C. Oerst according to the ratios of 0 g ： 6 g, 1 g ： 5 g, 2 g ： 4 g, 3 g ： 3 g, 4 g ： 2 g, 5 g ： 1 g and 6 g ： 0 g, and the mixtures were put in the re- spanse arm of Y-tube olfactometer as odor source to observe the olfactory behavior of T. yunnanensis, the empty arm was set as control. [ Result ] When the needles of P. yunnanensis were mixed with the leaves of non-hest plants according to the ratio of 1 g ： 5 g and 2 g ： 4 g, they had less difference on attractive rate to T. yun- nanensis compared with complete P. yunnanensis needles in mixture （ratio： 6 g ： 0 g）, and the maximum difference was 14%. When the needles ofP. yunnanen- sis were mixed with non-host leaves according to the ratio of 1 g ： 5 g, 2 g ： 4 g, the attractive rate to T. yunnanensis decreased compared with complete P. yun- nanensis needles in mixture （ ratio： 6 g ： 0 g）, and the decrease value in maximum was 40%. [ Conclusion] When the ratio of non-host plants was relatively small in mixed leaves, non-host had less impact on olfactory responses of T. yunnanensis. As the proportion of non-hest leaves gradually increased, the attractive rate of leaf mixtures to T. yunnanensis was gradually small. The results could provide reference for determination of mixed ratio in construction of mixed forest and the de- velopment of botanical attractive and repellent of T. yunnanensis.

小针刀对肌张力增高大鼠背根神经节PN3和NaN表达的影响

目的观察小针刀疗法对肌张力增高大鼠背根神经节内PN3和NaN表达的影响。方法24只sD大鼠随机分为空白组、对照组和针刀组，采用类似股薄肌肌张力增高方法进行造模。对大鼠0～14天的机械痛阚进行连续测定，间断测量热痛阈。采用荧光定量PCR法对大鼠背根神经节PN3和NaN基因mRNA转录水平进行对比分析。结果针刀组第9天以后的机械痛阈与对照组比较，差异有统计学意义（P〈0．01），小针刀疗法明显改善了肌张力增高大鼠的机械痛阈。与对照组比较，针刀组第10天热痛阈改善明显（P〈0．05）。与空白组比较，对照组PN3mRNA明显上调（P〈0．01），小针刀针刺之后，PN3mRNA上调趋势得到阻止（P〈0．05）。结论小针刀疗法镇痛的分子通路与阻止电压门控钠通道PN3mRNA的上调相关。

坐骨神经压迫性损伤大鼠背根神经节NaN/SNS2钠通道表达的变化

目的 :观察电压门控钠通道NaN /SNS2在大鼠坐骨神经慢性压迫性损伤 (chronicconstric tioninjury ,CCI) 14天后的变化 ,探讨慢性神经痛的发生机制。方法 :以逆转录多聚酶链反应(RT PCR)半定量分析CCI后大鼠背根神经节 (Dorsalrootgamglion ,DRG)钠通道NaN/SNS2转录物的变化 ,以及全细胞膜片钳技术记录CCI对急性分离大鼠背根神经节TTX R钠电流的影响。结果 :CCI术后 14天 ,感觉神经元特异性的TTX R钠通道转录物NaN/SNS2下调 ,与对照组相比 ,下降了大约 30 % ,TTX R钠电流密度明显减弱 ,但不影响其激活与稳态失活。其激活曲线的V1/2 分别为 - 2 6 .6 378mV、- 2 6 .6 30 6mV ,k为 6 .786 4mV、6 .732 3mV ;失活曲线的V1/2 分别为 -35 .5 84 4mV、- 37.2 188mV ,k为 8.2 792mV、8.4 6 47mV。结论 :钠通道NaN/SNS2与慢性神经痛后初级感觉神经元过度兴奋有关。

SG-NAN网络中的中继选择及其数量优化方法

邻域网(Neighborhood Area Network,NAN)中数据传输的可靠性是智能电网(Smart Grid,SG)研究领域的研究热点,但鲜有将SG-NAN中数据传输的可靠性与整体传输数据率的优化问题结合。因此,结合SG-NAN中整体传输功率的优化研究现状,通过固定源功率的功率分配算法,得出单个中继节点中继转发下的整体传输功率的最小化与中继节点位置选择之间的关系,并在此基础上进一步分析研究了多级协作通信组成的SG-NAN网络中传输数据率的最大化与中继节点数量之间的关系。仿真及分析结果表明,在保证可靠性的前提下,中继节点的选择和数量的优化能够实现SG-NAN网络中整体传输功率最小化和整体传输数据率的最大化。

NaN_3在苹果砧木组培苗诱变耐盐筛选中的应用

以苹果砧木试管继代苗为试材 ,用化学诱变剂NaN3对其叶片进行诱变处理 ,研究结果发现NaN3对叶片再生有明显影响 ,其存活率、再生率和再生芽数均比对照有所下降 ;NaN3处理的离体叶片再生植株经NaCl筛选后 ,其侧芽萌发数和相对生物产量与对照相比有一定程度的升高 。

肺炎链球菌主要毒力蛋白神经氨酸酶NanA的基本特性研究

目的研究肺炎链球菌主要毒力蛋白神经氨酸酶Nan A的基本特性,为新型疫苗研究打下基础。方法分离、纯化和鉴定重组Nan A;利用生物信息学和蛋白分析软件探索Nan A的蛋白特性。结果 Nan A全长1 039个氨基酸,其功能单位为第316-791位氨基酸片段,活性与全长Nan A无差异;Nan A片段水溶性和稳定性佳;Nan A蛋白表面负性电荷为主,活性中心以正性电荷为主,Tyr752或Asp372突变即可令其失活。结论Nan A可作为新型肺炎链球菌疫苗研发的新靶点。

Research on Flora in Qingxiu Mountain Scenic Area in Nanning City

Qingxiu Mountain Scenic Area is a famous tourism destination in Nanning City.Based on site survey,identification and data sorting for many years,the features of plant flora in Qingxiu Mountain Scenic Area were analyzed as below:① There are totally 1 431 species of vascular plant species,belonging to 752 genera and 174 families(including varieties,cultivated variations and variants,the same as below),among which there are 48 species of fern,belonging to 36 genera and 21 families,42 species of gymnosperm,belonging to 14 genera and 8 families,1 341 species of angiosperm,belonging to 702 genera and 145 families;② There are diversified species dominated by introduced and cultivated species;③ Relatively simple geographical components,mostly are tropical and subtropical regions;④ Being located in the transitional zone of tropical and subtropical regions with obvious tropical marginality;⑤ Most of the introduced species are rare and endangered ones,rarely are unique or ancient species.

Study on the geochemical characteristics of ocean-ridge and oceanic-island volcanic rocks in the Nan-Uttaradit zone, northern Thailand

Field investigations and laboratory integrated research as indicated that ophiolite mélange in the Nan-Uttaradit zone, northern Thailand, consists of fragments of tectonites such as metamorphic peridotite (extremely silicified serpentinite), cumulates (pyroxenolite, gabbro, and gabbro-diorite), ocean-ridge basalt, oceanic-island ba-salt and radiolarian silicalite, and it was formed during D3-P. The rock series, rock types and petrogeochemical characteristics of metamorphic tholeiites in the Nan area of the Nan-Uttaradit zone are similar to those of ocean-ridge basalts (C1) in China's Ailaoshan zone. As for the Hawaiites in the Nan area of the Nan-Uttaradit zone, their major elements, REEs and trace elements are similar to those of oceanic-island basalts in China's Jinshanjiang zone (P11). In the Uttaradit area of this zone the metamorphic alkaline basalts show transitional petrogeochemical characteristics between ocean-ridge basalts and oceanic-island basalts, which were still formed in oceanic-island environments. The above-described basalts are all oceanic volcanic rocks and they are the most important part of the Paleo-Tethys oceanic crust in the Nan-Uttaradit zone.

NaN_3诱变对赤小豆种子萌发与幼苗抗氧化系统的影响

叠氮化钠(NaN_3)诱变是获得基因水平变异体的重要途径,了解叠氮化钠处理时间和处理浓度等基本条件是进行诱变育种的前提。本研究采用0.0,0.2,0.6,1.0,1.4,1.8mmol/L的叠氮化钠溶液浓度对赤小豆种子进行诱变处理,处理时间为16,20,24h。通过发芽率及相对发芽率确定叠氮化钠诱变赤小豆的适宜浓度和时间(半致死剂量LD 50)。此外,还对赤小豆M1代幼苗中超氧化物歧化酶(SOD)、过氧化物酶(POD)以及过氧化氢酶(CAT)活性进行检测,探究叠氮化钠对赤小豆幼苗抗氧化系统的影响。结果表明:叠氮化钠对赤小豆诱变处理时间为20h时的半致死浓度为1.0mmol/L。叠氮化钠对赤小豆幼苗的SOD、POD活性表现为低浓度促进,高浓度抑制;对赤小豆幼苗的CAT活性有促进作用。当叠氮化钠浓度为1.0mmol/L时,赤小豆幼苗的SOD活性最高;而浓度为0.6mmol/L时,POD活性最高。

PREPARATION OF MULTI-WALLED CARBON NANOTUBES USING NiO CATALYST SYNTHESIZED BY HYDROTHERMAL METHOD

The Ni(OH)2/SiO2 binary colloid was prepared using Ni(NO3)2.6H2O and (C2H5O)4SiO4 as starting materials and was used to form NiO/SiO2. composite powder by hydrothermal method and desiccant method in open air respectively. Multi-walled carbon nanotubes (MWCNTs) were synthesized respectively by chemical vapor deposition using the NiO/SiO2 catalyst prepared by different methods. The phase and morphology of the catalysts and the morphology, output yield and purity of MWCNTs were compared by XRD, TEM and SEM. The results show that the catalyst powder prepared by hydrothermal method, compared with that by desiccant method, is smaller, better dispersion and has stronger catalytic activity. Pure MWCNTs with smaller tube diameter and narrow range could be obtained at a high yield using that NiO/SiO2 powder prepared by hydrothermal method as catalyst.

Using a water-confined carbon nanotube to probe the electricity of sequential charged segments of macromolecules

The detection of macromolecular conformation is particularly important in many physical and biological applications. Here we theoretically explore a method for achieving this detection by probing the electricity of sequential charged segments of macromolecules. Our analysis is based on molecular dynamics simulations, and we investigate a single file of water molecules confined in a half-capped single-walled carbon nanotube （SWCNT） with an external electric charge of ＋e or -e （e is the elementary charge）. The charge is located in the vicinity of the cap of the SWCNT and along the centerline of the SWCNT. We reveal the picosecond timescale for the re-orientation （namely, from one unidirectional direction to the other） of the water molecules in response to a switch in the charge signal, -e -＋ ＋e or ＋e -＋ --e. Our results are well understood by taking into account the electrical interactions between the water molecules and between the water molecules and the external charge. Because such signals of re-orientation can be magnified and transported according to Tu et al. [2009 Proc. Natl. Aead. Sci. USA 106 18120], it becomes possible to record fingerprints of electric signals arising from sequential charged segments of a macromolecule, which are expected to be useful for recognizing the conformations of some particular macromolecules.

Synthesis, Crystal Structure and Cytotoxic Activity of a New NAN-190 Analogue

The NAN-190 analogue 2-(2-(2-(4-(2-methoxyphenyl)piperazin-1-yl)ethoxy)ethyl)isoindoline-1,3-dione(1, C23H27N3O4, Mr = 409.48) was synthesized via a three-step reaction and characterized by 1H NMR, 13 C NMR, ESIMS and single-crystal X-ray diffraction. The crystal belongs to orthorhombic, space group Pna21 with a = 7.6445(15), b = 38.851(8), c = 7.1316(14) A, V = 2118.0(7) A3, Z = 4, Dc = 1.2840 mg/mm3, μ = 0.089 mm-1, F(000) = 872.4, R = 0.0545 and w R = 0.1681. The single-crystal X-ray structural analysis reveals that the piperazine ring in compound 1 presents a stable and minimum energy chair conformation. In addition, the preliminary cytotoxic assay shows that the title compound exhibits strong and selective inhibitory activity against DU145 cells(IC50 = 5.88 ± 1.02 μM).

基于NaN-Bicluster SMOTE的非均衡信贷数据分类研究及应用

为了有效评估非均衡信贷数据中的借款人信用风险,基于合成少数过采样技术(Synthetic minority oversampling technique,SMOTE)、自然近邻(Natural neighbor,NaN)和双聚类(Bicluster)构建了NaN?Bicluster SMOTE方法以改进SMOTE。首先使用无参数的自然近邻设定采样样本选取的逻辑规则,规避了r近邻划分样本时产生的不稳定性;其次基于自然近邻稳定结构规定安全范围设定的逻辑规则,避免合成样本成为噪声样本;然后使用双聚类挖掘局部规则,以合成样本继承局部规则的方式改进SMOTE合成公式;最后,在Prosper小额贷款平台的非均衡信贷数据集上将NaN?Bicluster SMOTE与若干采样方法和机器学习模型进行对比分析,并进一步使用统计检验方法验证其性能的优越性。

Evaluation of Heavy Metals Contamination on Representative Fruits in Nanning

[ Objective] The research aimed to analyze the contents of heavy metals in the typical fruits of Nanning and evaluate the pollution status of heavy metals in fruits of Nanning. [ Method] Five kinds of typical fruits （litchi, longan, dragon fruit, mangosteen and pineapple） in Nanning were collected to measure contents of heavy metals including Cd, Pb, As and Hg. According to standard of pollution-free fruits （GB18406.2 -2001 ）, pollution evaluation was conducted, and cumulative degrees of different fruits on a variety of heavy metals were contrasted. [ Result ] The heavy metal contents of all fruits were qualified. Comprehensive pollution index of dragon fruit was the maximum, and its cumulative capability on various heavy metals was the strongest. [ Conclusion] The pollution degrees of all fruits were safety in Nanning, and pollution level was clean.

Research on Plant Diversity of Street Greening in Nanning

Based on the research of plant diversity of 50 roads and the analysis of the plant species richness and composition,some aspects are compared:the frequency of species occuring,the diversity of evergreen plants and native plants.The ornamental characteristics diversity of plants are analyzed,and the inadequate aspects are proposed to make some countermeasures.

Preparation of hypocrellin B nanoparticlesfor photodynamic therapy

OBJECTIVE To prepare hypocrellin B-loaded apoferritin nanoparticles(HB-AFT NPs)for photodynamic therapy on tumor.METHODS HB-AFT NPs were prepared by taking advantage of the reversibleunfolding and refolding character of apoferritin in different pH environments.The photophysical and photobiological properties of hypocrellin B-loaded apoferritin were measured.RESULETS HB molecules were successfully encapsulated within apoferritincavity.HB-AFT-NPs exhibited higher ROS productionthan free HB.Additionally,phototoxicity of HB-AFT NPs to MDA-MB-231 cells was significantly improved as compared to free HB.CONCLUSION Together these results demonstrate that hypocrellin B-loaded apoferritin might be a potential nanoscale photosensitizer.