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Comprehensive analyses of the proteome and ubiquitome revealed mechanism of high temperature accelerating petal abscission in tree peony 被引量:1
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作者 Chunying Liu Ziqi Liu +4 位作者 Yanchao Yuan Yuxi Zhang Yifu Fang Junqiang Chen Shupeng Gai 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第1期205-222,共18页
Tree peony(Paeonia suffruticosa Andrews)is a well-known ornamental plant with high economic value,but the short fluorescence is a key obstacle to its ornamental value and industry development.High temperature accelera... Tree peony(Paeonia suffruticosa Andrews)is a well-known ornamental plant with high economic value,but the short fluorescence is a key obstacle to its ornamental value and industry development.High temperature accelerates flower senescence and abscission,but the associated mechanisms are poorly understood.In this study,the tandem mass tag(TMT)proteome and label-free quantitative ubiquitome from tree peony cut flowers treated with 20℃for 0 h(RT0),20℃or 28℃for 60 h(RT60 or HT60)were examined based on morphological observation,respectively.Totally,6970 proteins and 1545 lysine ubiquitinated(Kub)sites in 844 proteins were identified.Hydrophilic residues(such as glutamate and aspartate)neighboring the Kub sites were in preference,and 36.01%of the Kub sites were located on the protein surface.The differentially expressed proteins(DEPs)and Kub-DEPs in HT60 vs RT60 were mainly enriched in ribosomal protein,protein biosynthesis,secondary metabolites biosynthesis,flavonoid metabolism,carbohydrate catabolism,and auxin biosynthesis and signaling revealed by GO and KEGG analysis,accompanying the increase of endogenous abscisic acid(ABA)accumulation and decrease of endogenous indoleacetic acid(IAA)level.Additionally,the expression patterns of six enzymes(SAMS,ACO,YUC,CHS,ANS and PFK)putatively with Kub modifications were analyzed by proteome and real-time quantitative RT-PCR.The cell-free degradation assays showed PsSAMS and PsACO proteins could be degraded via the 26 S proteasome system in tree peony flowers.Finally,a working model was proposed for the acceleration of flower senescence and abscission by high temperature.In summary,all results contributed to understanding the mechanism of flower senescence induced by high temperature and prolonging fluorescence in tree peony. 展开更多
关键词 Tree peony PROTEOME Ubiquitome High temperature Flower senescence
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Changes in the root system of the herbaceous peony and soil properties under different years of continuous planting and replanting 被引量:4
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作者 Anqi Xie Limin Sun +4 位作者 Dongliang Zhang Yang Li Zemiao Liu Xue Li Xia Sun 《Horticultural Plant Journal》 SCIE CAS CSCD 2023年第4期801-810,共10页
The herbaceous peony(Paeonia lactiflora Pall.)has high ornamental value.Replanting problems occur when seedlings are replanted into previous holes.We studied the root system and soil environment of the'Dongjingnvl... The herbaceous peony(Paeonia lactiflora Pall.)has high ornamental value.Replanting problems occur when seedlings are replanted into previous holes.We studied the root system and soil environment of the'Dongjingnvlang'variety under a continuous planting regime of one,four,and seven years,and a replanting regime of one and four years.Under the condition of continuous planting,with the increase of number of years,pH,ammonium nitrogen,and nitrate nitrogen decreased in the rhizosphere and non-rhizosphere soils,whereas organic matter,available phosphorus and potassium,enzyme activities,and the number of bacteria,fungi,and actinomycetes increased.Under the condition of replanting,with the increase of number of years,fungi and actinomycetes in both soils increased,while pH,organic matter,nutrients,enzyme activities,and bacterial number decreased.pH,organic matter,nutrient content,enzyme activity and the number of bacterial were lower in soil replanted for four years,whereas the abundance of fungi and actinomycetes was higher,altering the soil from“bacterial high-fertility”to“fungal low-fertility”with increasing years of replanting.The activity of antioxidant enzymes and MDA content in roots of peony in replanting were higher than those in continuous planting,while the content of osmotic regulatory substances in replanting was lower than that in continuous planting.The results showed that there were no obvious adverse factors in soil during seven years of continuous planting,and herbaceous peony could maintain normal growth and development.However,soils after four years of replanting were not suitable for herbaceous peony growth.Benzoic acid increased with years of replanting,which potentially caused replanting problems.This study provides a theoretical basis for understanding the mechanism of replanting problems in the herbaceous peony. 展开更多
关键词 Herbaceous peony Replanting problems Continuous planting Soil environment Phenolic acids PAEONIFLORIN
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Osmotic Regulation,Antioxidant Enzyme Activities and Photosynthetic Characteristics of Tree Peony(Paeonia suffruticosa Andr.)in Response to High-Temperature Stress
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作者 Zhipeng Sheng Jiasong Meng +1 位作者 Jun Tao Daqiu Zhao 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第11期3133-3147,共15页
Tree peony(Paeonia suffruticosa Andr.)is a traditional Chinese flower,which prefers cool weather.However,high temperature in summer in the middle and lower reaches of the Yangtze River restricts its growth and develop... Tree peony(Paeonia suffruticosa Andr.)is a traditional Chinese flower,which prefers cool weather.However,high temperature in summer in the middle and lower reaches of the Yangtze River restricts its growth and development.In this study,osmotic regulation,antioxidant enzyme activities,and photosynthetic characteristics of tree peony in response to high-temperature stress were investigated.The results showed that high-temperature stress had destroyed the cell membrane,manifested as the increased relative electrical conductivity and malondialdehyde content.Moreover,high-temperature stress led to excessive accumulation of reactive oxygen species,thereby,activating antioxidant enzyme activities.Also,photosynthetic parameters and chlorophyll fluorescence parameters directly reflected the damage to the photosystem II reflection center under high-temperature stress.In addition,high-temperature stress led to stomatal closure and chloroplast damage.This study revealed the physiological responses of tree peony to high-temperature stress,laying a foundation for the promotion of tree peony in high-temperature areas and the improvement of high-temperature resistance. 展开更多
关键词 Tree peony high-temperature stress antioxidant enzyme photosynthetic characteristics
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Selection of Stable Reference Genes for Quantitative Real-Time PCR on Herbaceous Peony (Paeonia lactiflora Pall.) in Response to Drought Stress
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作者 Zhipeng Sheng Yuting Luan +2 位作者 Cong Xu Jun Tao Daqiu Zhao 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第3期801-814,共14页
Herbaceous peony(Paeonia lactiflora Pall.),as a high-end cut flower in the international market,has high ornamental and medicinal values.But in Northern China,drought is a major environmental factor influencing the gr... Herbaceous peony(Paeonia lactiflora Pall.),as a high-end cut flower in the international market,has high ornamental and medicinal values.But in Northern China,drought is a major environmental factor influencing the growth and development of P.lactiflora.Quantitative real-time polymerase chain reaction(qRT-PCR)can evaluate gene expression levels under different stress conditions,and stable internal reference is the key for qRT-PCR.At present,there is no systematic screening of internal reference for correcting gene expressions of P.lactiflora in response to drought stress.In this study,10 candidate genes[ubiquitin(UBQ2),UBQ1,elongation factor 1-α(EF-1α),Histidine(His),eukaryotic initiation factor(eIF),tubulin(TUB),actin(ACT),UBQ3,ACT2,RNA polymerase II(RNA Pol II)]were chosen,and 4 analysis methods were used to compare the stabilities for these 10 genes coping with drought stress.Due to the difference of operation methods,the results of different analysis were distinct,and the final comprehensive analysis indicated that EF-1αwas a relatively stable internal reference gene for P.lactiflora under drought stress.Also,UBQ1 and UBQ2 were the best reference gene combination according to GeNorm analysis.This study will lay a foundation for screening the key genes of P.lactiflora in response to drought stress. 展开更多
关键词 Herbaceous peony QRT-PCR reference gene drought stress
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Phoenix dactylifera:traditional uses,chemical constituents,nutritional benefit and therapeutic effects
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作者 Ali Esmail Al-Snafi Mahdi Murshd Thuwaini 《Traditional Medicine Research》 2023年第4期14-28,共15页
Phoenix dactylifera(Fam:Arecaceae)is originated from the Mesopotamia.The date fruit,was eaten fresh,in various processed forms and dried.The fruits were used traditionally as general tonic,for the treatment of liver d... Phoenix dactylifera(Fam:Arecaceae)is originated from the Mesopotamia.The date fruit,was eaten fresh,in various processed forms and dried.The fruits were used traditionally as general tonic,for the treatment of liver diseases,memory disturbances,fever,inflammation,paralysis,loss of consciousness,nervous disorders and consumed by pregnant women before and after delivery.However,all parts of the plant were used for some purpose.Dates fruits were considered a complete diet and a very important item of food,with plenty of vitamins and minerals.It contained a wide range of secondary metabolites.It possessed many pharmacological effects included anticancer,antidiabetic,anti-inflammatory,antimicrobial,antiparasitic,antioxidant,anti-toxin,cardiovascular,hypolipidemic,gastrointestinal,immunomodullatory,neural,hepato and reno-protective,reproductive and wound healing effects.This review highlighted the chemical constituents,nutritional and pharmacological effects of Phoenix dactylifera. 展开更多
关键词 phoenix dactylifera TRADITIONAL constituents NUTRITION PHARMACOLOGY
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Synergistic Mixture of Cyperus esculentus, Phoenix dactylifera and Cocos nucifera Aqueous Extract: Its Liver and Kidney Benefits in Male Albino Rat Model
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作者 Tamuno-Boma Odinga Cletus Barizoge Lemii +4 位作者 Iyaeneomi Ransome Daka Christine U. Gabriel-Brisibe Sarah K. Enebeli Iyingiala Austin-Asomeji Felicia Ucheawaji Edward 《Journal of Biosciences and Medicines》 2023年第10期63-75,共13页
Background: The synergistic mixture of Cyperus esculentus, Phoenix dactylifera and Cocos nucifera (STCD) aqueous extract is a common drink in Port Harcourt, Nigeria. It is assumed to have various health benefits. Howe... Background: The synergistic mixture of Cyperus esculentus, Phoenix dactylifera and Cocos nucifera (STCD) aqueous extract is a common drink in Port Harcourt, Nigeria. It is assumed to have various health benefits. However, the synergistic mixture of the content has not been studied scientifically, hence the need to evaluate its effect on the liver and kidney being part of the body’s metabolic organs. Aim: This study evaluated the synergistic mixture of Cyperus esculentus, Phoenix dactylifera and Cocos nucifera (STCD) aqueous extract in male albino rats. Methods: Acute toxicity LD<sub>50</sub> of STCD was carried out, afterwards, fifteen male albino rats were grouped into three groups with 5 rats in each group;Control, 200 mg/kg, 400 mg/kg STCD. The rats were administered STCD orally 24 hourly, for 21 days, with feed and water ad libitum. At the end of the experiment, blood samples were collected for biochemical analysis of the liver and kidney biomarkers, while the liver and kidney tissues were harvested for histopathological examination using standard laboratory methods. Descriptive statistics were computed and expressed as Mean ± SD. One-way ANOVA and Turkeys test was performed. P value ≤0.05 was considered statistically significant. Results: Acute toxicity LD50 of STCD was observed to be ≥2404.2 mg/kg body weight. An increase in the percentage body weight difference of 8.39% and 2.86% was observed for 200 and 400 mg/kg STCD groups. Also, the liver weight was observed to increase in 400 mg/kg (3.92 ± 1.42) in comparison to the control group (3.48 ± 1.61), a decrease in the kidney weight was observed in all groups administered STCD in comparison to the control group. Administration of STCD at both 200 and 400 mg/kg revealed a decrease in the concentration of the hepatic biomarkers AST, ALT, ALP, TP, Albumin, Total and conjugated bilirubin. The kidney biomarker Urea was observed to decrease in concentration for 200 mg/kg STCD (4.60 ± 1.83) and 400 mg/kg STCD (4.76 ± 0.74) when compared to the control group (6.32 ± 2.74). A decrease in Creatinine was observed in 200 mg/kg (91.80 ± 34.69) and 400 mg/kg (98.60 ± 15.53) in comparison to the control group (117.60 ± 42.88). The histological examination of the liver of rats administered STCD revealed structural normal central vein, hepatocytes and portal tract. The kidney examination revealed normal glomeruli and normal tubule. Conclusion: The findings of this study opine that STCD improved the health of both the liver and kidney as evidenced via the biomarkers and histological examinations of the liver and kidney. This study therefore recommends the intake of STCD at moderate doses for improved liver and kidney function due to its bioactive compounds and nutritional content. 展开更多
关键词 Cyperus esculentus (Tiger nuts) phoenix dactylifera (Dates) and Cocos nucifera (Coconuts) LIVER KIDNEY Male Rats
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Research on the Development Status of Oil-use Peony Industry in Heze City
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作者 Menglu GUO Ruiming BI 《Agricultural Biotechnology》 CAS 2023年第3期37-41,共5页
The peony industry is a characteristic industry in Heze City,and oil-use peony is an emerging industry.Heze City has planned oil-use peony as a key support and development industry in the local area.The oil-use peony ... The peony industry is a characteristic industry in Heze City,and oil-use peony is an emerging industry.Heze City has planned oil-use peony as a key support and development industry in the local area.The oil-use peony industry is full of treasures,but there are still many urgent problems to be solved for its sustained and healthy development:cultivation and planting,variety selection,product processing,and enterprise development,which have hindered the development of the oil-use peony industry.This paper delved into the comprehensive benefits of oil-use peony and its industrial development,providing some reference for the further development of the oil-use peony industry in Heze. 展开更多
关键词 Oil-use peony Heze City Utilization value Problem
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Research on Sustainable Development Strategies for Oil-use Peony in Heze City
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作者 Menglu GUO Ruiming BI 《Agricultural Biotechnology》 CAS 2023年第4期30-32,57,共4页
Oil-use peony is a treasure with broad prospects.Heze City plans to develop oil-use peony as a local key industry.However,there are still many urgent problems that need to be solved for the sustained and healthy devel... Oil-use peony is a treasure with broad prospects.Heze City plans to develop oil-use peony as a local key industry.However,there are still many urgent problems that need to be solved for the sustained and healthy development of the oil-use peony industry.This paper delved into the comprehensive benefits of oil-use peony and its industrial development from the aspects of cultivating high-quality germplasm resources,technological innovation,policy support,developing leading enterprises,improving industrialization system,comprehensive promotion,and strengthening storage and transportation technology,aiming to provide some reference for further development of Heze oil-use peony industry. 展开更多
关键词 Oil-use peony Heze City Utilization value Problem
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研华SSD携手Phoenix开发基于UEFI的安全解决方案
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《单片机与嵌入式系统应用》 2023年第11期96-96,共1页
研华与BIOS固件专家Phoenix合作推出基于UEFI(Unified Extensible Firmware Interface,统一可扩展固件接口)的SSD安全软件工具---SQErase。SQErase旨在开创数据安全的未来,可通过UEFI删除关键数据,不受操作系统的限制,为坚固耐用型工业... 研华与BIOS固件专家Phoenix合作推出基于UEFI(Unified Extensible Firmware Interface,统一可扩展固件接口)的SSD安全软件工具---SQErase。SQErase旨在开创数据安全的未来,可通过UEFI删除关键数据,不受操作系统的限制,为坚固耐用型工业应用提供了全面的存储安全解决方案。研华SSD SQFlash系列产品为军事、金融、医疗保健、交通和政务应用提供了更高的安全性和可靠性。 展开更多
关键词 安全解决方案 软件工具 数据安全 UEFI phoenix FLASH 固件 SSD
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叶面喷施胺鲜酯及其与油菜素内酯复配液对油用牡丹生理特性及产量的影响 被引量:1
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作者 李晓鹏 肖瑞雪 +6 位作者 张利霞 张亚冰 余世玺 陈轲 李仁杰 罗宁 侯小改 《山东农业科学》 北大核心 2024年第1期97-104,共8页
为筛选出适合油用牡丹高产高效栽培中的植物生长调节剂应用技术,本试验以凤丹为材料,分别于盛花期(4月7日)、籽粒形成期(4月18日)、果荚与籽粒快速生长期(5月10日)叶面喷施不同质量浓度的胺鲜酯(D1、D2和D3)及其与油菜素内酯复配液(BD1... 为筛选出适合油用牡丹高产高效栽培中的植物生长调节剂应用技术,本试验以凤丹为材料,分别于盛花期(4月7日)、籽粒形成期(4月18日)、果荚与籽粒快速生长期(5月10日)叶面喷施不同质量浓度的胺鲜酯(D1、D2和D3)及其与油菜素内酯复配液(BD1、BD2和BD3),研究其对植株生理特性、籽粒产量指标(百粒重、单果籽粒重、单株籽粒重及籽粒产量)的影响。结果表明,与对照组相比,喷施植物生长调节剂处理的牡丹叶片生理特性和籽粒产量关键参数均有明显提高:7月3日时,BD2处理叶片SOD活性较对照显著提高5.24%;5月19日和7月3日时,所有处理叶片MDA含量均较对照显著降低;7月3日时,所有处理叶片可溶性糖含量与对照均有显著性差异,其中BD2处理最高,较对照增加33.84%;6月10日时,所有处理叶片总叶绿素含量均显著高于对照,其中BD3处理增幅最大,为98.11%;3个测定时期,除6月10日的BD3处理外其他处理叶片净光合速率均显著高于对照,且随着胺鲜酯质量浓度的提高呈现先增加后降低趋势。所有处理单株籽粒重及籽粒产量均显著高于对照,其中BD3增幅均最大,分别为10.45%与10.44%。相关性分析表明,SOD活性与Chl a、Chl b、Car、总叶绿素含量和净光合速率呈极显著正相关,与可溶性糖含量呈显著正相关,与MDA含量呈极显著负相关;总叶绿素含量与净光合速率呈极显著正相关,净光合速率与单果籽粒重、单株籽粒重和籽粒产量呈显著正相关。综之,12、16 mg/L胺鲜酯与0.05 mg/L油菜素内酯复配喷施油用牡丹植株的生理特性表现优异,并可获得较高籽粒产量。 展开更多
关键词 油用牡丹 凤丹 胺鲜酯 油菜素内酯 生理特性 籽粒产量
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22个芍药品种种籽功能性成分分析 被引量:1
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作者 王浩 魏硕 +4 位作者 何莹龙 马慧丽 李昱莹 郭丽丽 侯小改 《安徽农业科学》 CAS 2024年第5期195-200,共6页
以22个芍药品种为试验材料,探究其出仁率、出油率以及种籽中籽油、籽粕和种皮中的功能性成分和含量。结果表明,通过超临界流体萃取仪提取芍药籽油,测得参试品种出油率为15.96%~26.62%,平均为22.12%;经气相色谱-质谱联用仪分析表明,参试... 以22个芍药品种为试验材料,探究其出仁率、出油率以及种籽中籽油、籽粕和种皮中的功能性成分和含量。结果表明,通过超临界流体萃取仪提取芍药籽油,测得参试品种出油率为15.96%~26.62%,平均为22.12%;经气相色谱-质谱联用仪分析表明,参试芍药籽油中的脂肪酸主要为油酸、亚麻酸、亚油酸、硬脂酸、棕榈酸等,籽油中的总不饱和脂肪酸超过85%,营养价值和保健功能较高,具有开发利用的潜力;芍药籽油榨取后副产物籽粕中总黄酮、芍药苷和粗蛋白的含量分别为7.78 mg/g、24.85 mg/g和16.92%,种皮中总黄酮和白藜芦醇的含量分别为107.30和3.11 mg/g。综上所述,籽粕和种皮既可作为功能性营养保健品的原料开发利用,也可作为新型高营养饲料使用,表明芍药具有较高的综合利用潜力,能够为相关产业的发展提供重要参考。 展开更多
关键词 芍药 籽油 籽粕 种皮 功能性成分 含量
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芍药根茎形成发育过程中内源激素和碳水化合物的变化
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作者 王慧娟 王二强 +6 位作者 符真珠 李艳敏 王晓晖 袁欣 高杰 王利民 张和臣 《河南农业科学》 北大核心 2024年第3期118-124,共7页
为了明确芍药根茎形成发育过程中内源激素和碳水化合物的变化趋势,以芍药实生苗为试验材料,观测根茎初始形成和膨大发育期的形态变化,并对其内源激素及碳水化合物含量的变化进行研究。结果表明,根茎的形成和发育可划分为4个时期,分别为... 为了明确芍药根茎形成发育过程中内源激素和碳水化合物的变化趋势,以芍药实生苗为试验材料,观测根茎初始形成和膨大发育期的形态变化,并对其内源激素及碳水化合物含量的变化进行研究。结果表明,根茎的形成和发育可划分为4个时期,分别为营养生长期(Ⅰ)、根茎转换期(Ⅱ)、膨大期(Ⅲ)、膨大后期(Ⅳ)。根茎形成发育前期差异激素较多,其中,根茎转换期(Ⅱ)ABA和GA3含量急剧升高,达到峰值,分别为94.96、541.53 ng/g,随后显著下降,表明二者共同调节了根茎的初始形成。生长素类和细胞分裂素类在根茎转换期(Ⅱ)和膨大期(Ⅲ)表达量较高,对根茎的发育和膨大起主导作用。葡萄糖和果糖等还原糖含量在根茎转换期(Ⅱ)显著下降后保持在较低水平,蔗糖和淀粉含量则随根茎的形成和发育呈逐渐上升趋势,是根茎储存和积累的主要碳水化合物。 展开更多
关键词 芍药 根茎 内源激素 碳水化合物
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S7-1500 PLC与Phoenix Quadro EU检漏仪通讯的研发与应用
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作者 孟丽红 张川 王辉 《中国科技期刊数据库 工业A》 2023年第9期182-187,共6页
在压氦及氦检漏装置生产线中配置了3个检漏仪,检漏仪型号为Phoenix Quadro EU,品牌为德国莱宝,分别用于检漏装置A、检漏装置B和人工检漏系统,测试系统本底和工件的漏率。以往项目检漏仪漏率数据是通过检漏仪与工控机组态软件通讯,将数... 在压氦及氦检漏装置生产线中配置了3个检漏仪,检漏仪型号为Phoenix Quadro EU,品牌为德国莱宝,分别用于检漏装置A、检漏装置B和人工检漏系统,测试系统本底和工件的漏率。以往项目检漏仪漏率数据是通过检漏仪与工控机组态软件通讯,将数据在组态软件上显示,漏率是检漏系统的关键数据,并有大量的数据处理需要完成,这种方式的缺点是:生成的数据有时和实际数据会有差异,这个跟组态软件采集周期有关;数据处理有时会造成数据丢失,导致数据记录和结果不可靠,这个和组态软件采集不到触发信号有关;一旦发生数据没有记录的情况,需要重新进行检漏测试,造成时间和资源的浪费,而且对于系统的可靠性也存在质疑。本项目控制系统采用西门子公司的S7-1500系列PLC,采用检漏仪直接和PLC进行通讯的方式采集某一时刻检漏仪漏率数据,将数据存入数据库生成生产报表,并将结果在监控系统组态软件上显示。实际测试结果表明数据和结果可靠,工作效率高,系统可靠性高。 展开更多
关键词 通讯 检漏仪 西门子S7-1500 phoenix QUADRO 检漏 莱宝
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S-8大孔树脂-C18柱联用分离牡丹籽壳中短叶松素及抗氧化研究
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作者 张迎阳 邹林玲 +4 位作者 陈文涛 孙承骏 邹平 徐莹 满在伟 《食品与发酵工业》 CAS CSCD 北大核心 2024年第6期152-158,共7页
以牡丹籽壳为原料,利用大孔树脂和C18反相键合硅胶柱(C18柱)联合分离短叶松素,回收率为(98.33±0.64)%。用70%(体积分数)乙醇提取牡丹籽壳粗黄酮(Mu Dan Ke Flavonoids,MDKF),得率最高为(10.54±0.13)%;比较了6种大孔树脂(AB-8... 以牡丹籽壳为原料,利用大孔树脂和C18反相键合硅胶柱(C18柱)联合分离短叶松素,回收率为(98.33±0.64)%。用70%(体积分数)乙醇提取牡丹籽壳粗黄酮(Mu Dan Ke Flavonoids,MDKF),得率最高为(10.54±0.13)%;比较了6种大孔树脂(AB-8、S-8、DM301、HPD600、HPD100和D101)的吸附率和解析率,发现S-8大孔树脂的吸附率和解析率最佳,分别为83.47%和84.46%;优化S-8大孔树脂分离MDKF的最佳条件为:上样液质量浓度1.6 mg/mL,上样液流速2.0 mL/min,洗脱剂乙醇体积分数60%,洗脱液流速1.5 mL/min,洗脱液体积100 mL;对经过C18柱分离后的组分进行LC-MS分析得到短叶松素,回收率为(98.33±0.64)%;分离前后抗氧化能力比较:C18纯化物>S-8大孔树脂纯化物>MDKF粗提物;分子对接实验表明,短叶松素与超氧化物歧化酶、过氧化氢酶、过氧化物酶、谷胱甘肽过氧化物酶均有结合能力,过氧化氢酶的结合能力最强为-9.1 kcal/mol。实验表明,短叶松素具有较好的抗氧化能力,可作为食品、药品或化妆品等抗氧化剂的添加,应用前景非常广泛,对牡丹籽壳的进一步开发利用提供理论支持。 展开更多
关键词 牡丹籽壳 短叶松素 大孔树脂 C18 分子对接
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基于可视化思维探讨白芍炮制历史沿革及临床应用研究
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作者 张晓鸣 刘产明 +3 位作者 朱月琴 华丰 戴亦娴 高毕华 《中医药学报》 CAS 2024年第7期36-41,共6页
目的:基于可视化分析比较探讨白芍炮制及临床应用的历史与发展。方法:采用Excel软件对历代炮制方法、朝代、出处、现代炮制工艺及古今临床应用等信息进行提取和统计分析,采用Cytoscape3.7.1软件构成“白芍-炮制方法-朝代-出处”网络,采... 目的:基于可视化分析比较探讨白芍炮制及临床应用的历史与发展。方法:采用Excel软件对历代炮制方法、朝代、出处、现代炮制工艺及古今临床应用等信息进行提取和统计分析,采用Cytoscape3.7.1软件构成“白芍-炮制方法-朝代-出处”网络,采用Citespace 6.2.R4软件对纳入文献关键词进行可视化图谱分析。结果:白芍古今炮制方法以酒制和炒制为主,而其他大部分炮制方法现今面临失传的困境。纵观白芍炮制品的古今应用略有不同,其现代临床应用趋向于在传统“辨证”治疗的疾病观中结合具体病灶观。结论:白芍传统炮制方法与临床应用,既需要经验的传承,并在存同异中不断发展,又需要现代政策的引导和创新支撑,才能切实实现特色炮制品种的开发与活态化临床转化应用。 展开更多
关键词 白芍 炮制 历史沿革 可视化 炮制作用 临床应用
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油用牡丹凤丹的单株产量构成要素
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作者 汪成忠 李庆魁 +4 位作者 尹原森 杨继 宋志平 吴计划 胡胜甜 《经济林研究》 北大核心 2024年第3期289-294,共6页
【目的】为促进凤丹优良种质筛选和高产品种培育提供参考。【方法】以6龄凤丹为研究对象,在果实开裂期,检测10个生长性状(主枝基径、主枝长、2年生枝基径、2年生枝长、小枝长、小枝基径、复叶数量、叶柄长、复叶长和复叶宽)和5个繁殖性... 【目的】为促进凤丹优良种质筛选和高产品种培育提供参考。【方法】以6龄凤丹为研究对象,在果实开裂期,检测10个生长性状(主枝基径、主枝长、2年生枝基径、2年生枝长、小枝长、小枝基径、复叶数量、叶柄长、复叶长和复叶宽)和5个繁殖性状(单果数量、单果长、种子量、败育单果荚数量和产量),分析各性状的相关性,采用逐步回归法筛选与产量相关的性状,再对所筛选的性状进行通径分析,明确其对产量的效应。【结果】凤丹的繁殖性状指标的变异系数大于生长性状。与产量显著相关的性状有种子数量、单果长、2年生枝基径、2年生枝长和小枝长;种子数量对种子产量的影响最大(相关系数R=0.811),其次为单果长(R=0.210),小枝长、2年生枝基径和2年生枝长通过影响种子数量和单果长来对种子质量产生影响。以产量(y)为因变量,以种子数量(x_(1))、单果长(x_(2))、小枝长(x_(3))、2年生枝基径(x_(4))为自变量,构建的凤丹单株产量模型为y=0.142x_(1)+1.123x_(2)+0.027x_(3)+0.014x_(1)x_(2)+0.011x_(1)x_(4)-0.043x_(2)x_(4)-3.386。【结论】单株产量构成的关键要素为种子数量(果实数量)和单果长(果实大小),说明凤丹的单株产量由“库”的大小所决定,同时受到“源”强度的影响。实际生产中在调“源”的同时,更应关注扩“库”增产措施的应用。 展开更多
关键词 油用牡丹 凤丹 单株产量 产量构成
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发酵牡丹籽粕对黄山黑鸡生产性能、蛋品质、蛋黄中脂肪酸含量及血清生化指标的影响
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作者 刘伟 陈相名 +7 位作者 马瑞钰 朱涌峰 戚仁荣 李俊营 万意 李岩 舒翔 詹凯 《动物营养学报》 CAS CSCD 北大核心 2024年第2期874-884,共11页
本试验旨在研究发酵牡丹籽粕对黄山黑鸡生产性能、蛋品质、蛋黄中脂肪酸含量及血清生化指标的影响。选取240日龄产蛋率及体重相近的黄山黑鸡864只,随机分为3组,每组4个重复,每个重复72只。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮... 本试验旨在研究发酵牡丹籽粕对黄山黑鸡生产性能、蛋品质、蛋黄中脂肪酸含量及血清生化指标的影响。选取240日龄产蛋率及体重相近的黄山黑鸡864只,随机分为3组,每组4个重复,每个重复72只。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加5%(5%FPSM组)和9%发酵牡丹籽粕(9%FPSM组)的试验饲粮。预试期1周,正试期8周。结果表明:1)与对照组相比,5%FPSM组的产蛋率显著升高(P<0.05),料蛋比和平均日采食量显著降低(P<0.05);9%FPSM组的产蛋率和平均日采食量极显著降低(P<0.01)。2)与对照组相比,9%FPSM组的蛋形指数显著升高(P<0.05),蛋壳颜色显著降低(P<0.05)。3)与对照组相比,5%FPSM组的蛋黄中ω-3多不饱和脂肪酸(PUFA)含量显著升高(P<0.05);9%FPSM组的蛋黄中总饱和脂肪酸(SFA)含量和ω-6/ω-3 PUFA显著或极显著降低(P<0.05或P<0.01),总PUFA和ω-3 PUFA含量显著或极显著升高(P<0.05或P<0.01)。4)与对照组相比,5%FPSM组的血清甘油三酯(TG)、免疫球蛋白A(IgA)、免疫球蛋白G(IgG)含量和碱性磷酸酶(ALP)活性及总抗氧化力(T-AOC)极显著升高(P<0.01),血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和免疫球蛋白M(IgM)含量极显著降低(P<0.01);9%FPSM组的血清低密度脂蛋白(LDL)、TG、尿素氮(UN)、IgA、IgM含量和ALP活性及T-AOC显著或极显著升高(P<0.05或P<0.01),血清高密度脂蛋白(HDL)和丙二醛(DMA)含量极显著降低(P<0.01)。由此可见,饲粮中添加5%的发酵牡丹籽粕可提高黄山黑鸡的生产性能;添加5%和9%的发酵牡丹籽粕可提高黄山黑鸡的蛋黄中ω-3 PUFA含量,提高机体抗氧化能力和免疫力。本试验条件下,黄山黑鸡饲粮中发酵牡丹籽粕的适宜添加比例为5%。 展开更多
关键词 发酵牡丹籽粕 黄山黑鸡 生产性能 蛋品质 脂肪酸 血清生化指标
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外源GA_(3)对“白雪塔”牡丹花期生理效应的影响
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作者 陶良如 米银法 《北方园艺》 CAS 北大核心 2024年第20期37-45,共9页
以观赏牡丹“白雪塔”为试材,采用植株喷施法,研究了外源赤霉素(GA_(3))对牡丹花期生理效应的影响,以期为牡丹产业中的催花调控、切花生产及旅游创收等方面提供参考依据。结果表明:外施500 mg·L-1GA_(3)可使“白雪塔”提前4~5 d开... 以观赏牡丹“白雪塔”为试材,采用植株喷施法,研究了外源赤霉素(GA_(3))对牡丹花期生理效应的影响,以期为牡丹产业中的催花调控、切花生产及旅游创收等方面提供参考依据。结果表明:外施500 mg·L-1GA_(3)可使“白雪塔”提前4~5 d开花且花期延长2~3 d,有效拓宽了观赏花期。同时还显著降低了幼叶和花瓣内脱落酸(ABA)含量,提高了GA_(3)、生长素(IAA)含量及IAA/ABA、GA_(3)/ABA、(IAA+GA_(3))/ABA比值。低含量的ABA和高比值的(IAA+GA_(3))/ABA是“白雪塔”花期延长的重要内部激素调控因素。此外,外施GA_(3)还显著降低了花期衰老进程中的超氧阴离子(O·2)产生速率和丙二醛(MDA)含量,提高了可溶性蛋白质含量及超氧化物歧化酶(SOD)活性。与各自对照相比,幼叶内可溶性蛋白质含量和SOD活性分别比对照提高了39.3%~118.2%、20.7%~38.3%;花瓣可溶性蛋白质含量和SOD活性则分别提高了43.0%~60.3%、23.9%~57.0%(P<0.05)。可溶性蛋白质含量及SOD活性的提高,有效缓解了因开花衰老诱发的过量活性氧对细胞膜脂造成的过氧化伤害,在抑制初花至败花期时的组织衰老方面,发挥了重要的防御作用。 展开更多
关键词 牡丹 外源GA_(3) 花期 生理效应 “白雪塔”
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文化生态学视角下“凤阳凤画”的文化根源及价值向度分
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作者 金灿 朱金鑫 《吉林艺术学院学报》 2024年第4期20-27,共8页
文化生态学认为生态环境与人类文化之间相互构建,生态环境塑造并影响着人类文化的产生和发展,人类文化活动也不断地影响和改变其所处的自然和社会环境。“凤阳凤画”是安徽凤阳地区的美术类非遗项目,自然环境为其诞生提供了创作灵感和素... 文化生态学认为生态环境与人类文化之间相互构建,生态环境塑造并影响着人类文化的产生和发展,人类文化活动也不断地影响和改变其所处的自然和社会环境。“凤阳凤画”是安徽凤阳地区的美术类非遗项目,自然环境为其诞生提供了创作灵感和素材,人文历史环境影响了其主题思想和画面风格,而社会文化环境则为它的传承和发展提供了支持和保障。立足文化生态学视角分析“凤阳凤画”的产生和发展,并从思想内涵、艺术表现及资源开发等多个维度探讨其价值,同时,对“凤阳凤画”的未来发展提出一些可供参考的建议,以期望促进“凤阳凤画”的传承与发展,为振兴乡村文化和经济的繁荣做出积极贡献。 展开更多
关键词 凤阳凤画 文化根源 价值向度 乡村振兴
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景观型木麻黄新品种′如意凤′
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作者 李茂瑾 叶功富 +4 位作者 陈胜 王小红 苏宝川 苏亲桂 郑焙华 《福建林业科技》 2024年第3期89-90,100,共3页
在福建省惠安赤湖国有防护林场国家木麻黄种质资源库中,经过长期观察发现特异性状的单株,然后对特异性状单株进行无性繁殖获得无性系苗木进行DUS测试,于2023年获得植物新品种的授权(品种名:如意凤,品种权号:20230152)。新品种′如意凤... 在福建省惠安赤湖国有防护林场国家木麻黄种质资源库中,经过长期观察发现特异性状的单株,然后对特异性状单株进行无性繁殖获得无性系苗木进行DUS测试,于2023年获得植物新品种的授权(品种名:如意凤,品种权号:20230152)。新品种′如意凤′的树冠呈圆锥形,主干树皮块状开裂如蛇纹,树皮灰褐色;齿状叶尖端红色,紧贴;符合植物新品种特异性、一致性和稳定性要求,具有较高的景观价值。研究结果可为滨海地区的绿化和美化提供种质资源。 展开更多
关键词 景观型 木麻黄 DUS测试 ′如意凤′
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