Tree peony(Paeonia suffruticosa Andrews)is a well-known ornamental plant with high economic value,but the short fluorescence is a key obstacle to its ornamental value and industry development.High temperature accelera...Tree peony(Paeonia suffruticosa Andrews)is a well-known ornamental plant with high economic value,but the short fluorescence is a key obstacle to its ornamental value and industry development.High temperature accelerates flower senescence and abscission,but the associated mechanisms are poorly understood.In this study,the tandem mass tag(TMT)proteome and label-free quantitative ubiquitome from tree peony cut flowers treated with 20℃for 0 h(RT0),20℃or 28℃for 60 h(RT60 or HT60)were examined based on morphological observation,respectively.Totally,6970 proteins and 1545 lysine ubiquitinated(Kub)sites in 844 proteins were identified.Hydrophilic residues(such as glutamate and aspartate)neighboring the Kub sites were in preference,and 36.01%of the Kub sites were located on the protein surface.The differentially expressed proteins(DEPs)and Kub-DEPs in HT60 vs RT60 were mainly enriched in ribosomal protein,protein biosynthesis,secondary metabolites biosynthesis,flavonoid metabolism,carbohydrate catabolism,and auxin biosynthesis and signaling revealed by GO and KEGG analysis,accompanying the increase of endogenous abscisic acid(ABA)accumulation and decrease of endogenous indoleacetic acid(IAA)level.Additionally,the expression patterns of six enzymes(SAMS,ACO,YUC,CHS,ANS and PFK)putatively with Kub modifications were analyzed by proteome and real-time quantitative RT-PCR.The cell-free degradation assays showed PsSAMS and PsACO proteins could be degraded via the 26 S proteasome system in tree peony flowers.Finally,a working model was proposed for the acceleration of flower senescence and abscission by high temperature.In summary,all results contributed to understanding the mechanism of flower senescence induced by high temperature and prolonging fluorescence in tree peony.展开更多
The herbaceous peony(Paeonia lactiflora Pall.)has high ornamental value.Replanting problems occur when seedlings are replanted into previous holes.We studied the root system and soil environment of the'Dongjingnvl...The herbaceous peony(Paeonia lactiflora Pall.)has high ornamental value.Replanting problems occur when seedlings are replanted into previous holes.We studied the root system and soil environment of the'Dongjingnvlang'variety under a continuous planting regime of one,four,and seven years,and a replanting regime of one and four years.Under the condition of continuous planting,with the increase of number of years,pH,ammonium nitrogen,and nitrate nitrogen decreased in the rhizosphere and non-rhizosphere soils,whereas organic matter,available phosphorus and potassium,enzyme activities,and the number of bacteria,fungi,and actinomycetes increased.Under the condition of replanting,with the increase of number of years,fungi and actinomycetes in both soils increased,while pH,organic matter,nutrients,enzyme activities,and bacterial number decreased.pH,organic matter,nutrient content,enzyme activity and the number of bacterial were lower in soil replanted for four years,whereas the abundance of fungi and actinomycetes was higher,altering the soil from“bacterial high-fertility”to“fungal low-fertility”with increasing years of replanting.The activity of antioxidant enzymes and MDA content in roots of peony in replanting were higher than those in continuous planting,while the content of osmotic regulatory substances in replanting was lower than that in continuous planting.The results showed that there were no obvious adverse factors in soil during seven years of continuous planting,and herbaceous peony could maintain normal growth and development.However,soils after four years of replanting were not suitable for herbaceous peony growth.Benzoic acid increased with years of replanting,which potentially caused replanting problems.This study provides a theoretical basis for understanding the mechanism of replanting problems in the herbaceous peony.展开更多
Tree peony(Paeonia suffruticosa Andr.)is a traditional Chinese flower,which prefers cool weather.However,high temperature in summer in the middle and lower reaches of the Yangtze River restricts its growth and develop...Tree peony(Paeonia suffruticosa Andr.)is a traditional Chinese flower,which prefers cool weather.However,high temperature in summer in the middle and lower reaches of the Yangtze River restricts its growth and development.In this study,osmotic regulation,antioxidant enzyme activities,and photosynthetic characteristics of tree peony in response to high-temperature stress were investigated.The results showed that high-temperature stress had destroyed the cell membrane,manifested as the increased relative electrical conductivity and malondialdehyde content.Moreover,high-temperature stress led to excessive accumulation of reactive oxygen species,thereby,activating antioxidant enzyme activities.Also,photosynthetic parameters and chlorophyll fluorescence parameters directly reflected the damage to the photosystem II reflection center under high-temperature stress.In addition,high-temperature stress led to stomatal closure and chloroplast damage.This study revealed the physiological responses of tree peony to high-temperature stress,laying a foundation for the promotion of tree peony in high-temperature areas and the improvement of high-temperature resistance.展开更多
Herbaceous peony(Paeonia lactiflora Pall.),as a high-end cut flower in the international market,has high ornamental and medicinal values.But in Northern China,drought is a major environmental factor influencing the gr...Herbaceous peony(Paeonia lactiflora Pall.),as a high-end cut flower in the international market,has high ornamental and medicinal values.But in Northern China,drought is a major environmental factor influencing the growth and development of P.lactiflora.Quantitative real-time polymerase chain reaction(qRT-PCR)can evaluate gene expression levels under different stress conditions,and stable internal reference is the key for qRT-PCR.At present,there is no systematic screening of internal reference for correcting gene expressions of P.lactiflora in response to drought stress.In this study,10 candidate genes[ubiquitin(UBQ2),UBQ1,elongation factor 1-α(EF-1α),Histidine(His),eukaryotic initiation factor(eIF),tubulin(TUB),actin(ACT),UBQ3,ACT2,RNA polymerase II(RNA Pol II)]were chosen,and 4 analysis methods were used to compare the stabilities for these 10 genes coping with drought stress.Due to the difference of operation methods,the results of different analysis were distinct,and the final comprehensive analysis indicated that EF-1αwas a relatively stable internal reference gene for P.lactiflora under drought stress.Also,UBQ1 and UBQ2 were the best reference gene combination according to GeNorm analysis.This study will lay a foundation for screening the key genes of P.lactiflora in response to drought stress.展开更多
Phoenix dactylifera(Fam:Arecaceae)is originated from the Mesopotamia.The date fruit,was eaten fresh,in various processed forms and dried.The fruits were used traditionally as general tonic,for the treatment of liver d...Phoenix dactylifera(Fam:Arecaceae)is originated from the Mesopotamia.The date fruit,was eaten fresh,in various processed forms and dried.The fruits were used traditionally as general tonic,for the treatment of liver diseases,memory disturbances,fever,inflammation,paralysis,loss of consciousness,nervous disorders and consumed by pregnant women before and after delivery.However,all parts of the plant were used for some purpose.Dates fruits were considered a complete diet and a very important item of food,with plenty of vitamins and minerals.It contained a wide range of secondary metabolites.It possessed many pharmacological effects included anticancer,antidiabetic,anti-inflammatory,antimicrobial,antiparasitic,antioxidant,anti-toxin,cardiovascular,hypolipidemic,gastrointestinal,immunomodullatory,neural,hepato and reno-protective,reproductive and wound healing effects.This review highlighted the chemical constituents,nutritional and pharmacological effects of Phoenix dactylifera.展开更多
Background: The synergistic mixture of Cyperus esculentus, Phoenix dactylifera and Cocos nucifera (STCD) aqueous extract is a common drink in Port Harcourt, Nigeria. It is assumed to have various health benefits. Howe...Background: The synergistic mixture of Cyperus esculentus, Phoenix dactylifera and Cocos nucifera (STCD) aqueous extract is a common drink in Port Harcourt, Nigeria. It is assumed to have various health benefits. However, the synergistic mixture of the content has not been studied scientifically, hence the need to evaluate its effect on the liver and kidney being part of the body’s metabolic organs. Aim: This study evaluated the synergistic mixture of Cyperus esculentus, Phoenix dactylifera and Cocos nucifera (STCD) aqueous extract in male albino rats. Methods: Acute toxicity LD<sub>50</sub> of STCD was carried out, afterwards, fifteen male albino rats were grouped into three groups with 5 rats in each group;Control, 200 mg/kg, 400 mg/kg STCD. The rats were administered STCD orally 24 hourly, for 21 days, with feed and water ad libitum. At the end of the experiment, blood samples were collected for biochemical analysis of the liver and kidney biomarkers, while the liver and kidney tissues were harvested for histopathological examination using standard laboratory methods. Descriptive statistics were computed and expressed as Mean ± SD. One-way ANOVA and Turkeys test was performed. P value ≤0.05 was considered statistically significant. Results: Acute toxicity LD50 of STCD was observed to be ≥2404.2 mg/kg body weight. An increase in the percentage body weight difference of 8.39% and 2.86% was observed for 200 and 400 mg/kg STCD groups. Also, the liver weight was observed to increase in 400 mg/kg (3.92 ± 1.42) in comparison to the control group (3.48 ± 1.61), a decrease in the kidney weight was observed in all groups administered STCD in comparison to the control group. Administration of STCD at both 200 and 400 mg/kg revealed a decrease in the concentration of the hepatic biomarkers AST, ALT, ALP, TP, Albumin, Total and conjugated bilirubin. The kidney biomarker Urea was observed to decrease in concentration for 200 mg/kg STCD (4.60 ± 1.83) and 400 mg/kg STCD (4.76 ± 0.74) when compared to the control group (6.32 ± 2.74). A decrease in Creatinine was observed in 200 mg/kg (91.80 ± 34.69) and 400 mg/kg (98.60 ± 15.53) in comparison to the control group (117.60 ± 42.88). The histological examination of the liver of rats administered STCD revealed structural normal central vein, hepatocytes and portal tract. The kidney examination revealed normal glomeruli and normal tubule. Conclusion: The findings of this study opine that STCD improved the health of both the liver and kidney as evidenced via the biomarkers and histological examinations of the liver and kidney. This study therefore recommends the intake of STCD at moderate doses for improved liver and kidney function due to its bioactive compounds and nutritional content.展开更多
The peony industry is a characteristic industry in Heze City,and oil-use peony is an emerging industry.Heze City has planned oil-use peony as a key support and development industry in the local area.The oil-use peony ...The peony industry is a characteristic industry in Heze City,and oil-use peony is an emerging industry.Heze City has planned oil-use peony as a key support and development industry in the local area.The oil-use peony industry is full of treasures,but there are still many urgent problems to be solved for its sustained and healthy development:cultivation and planting,variety selection,product processing,and enterprise development,which have hindered the development of the oil-use peony industry.This paper delved into the comprehensive benefits of oil-use peony and its industrial development,providing some reference for the further development of the oil-use peony industry in Heze.展开更多
Oil-use peony is a treasure with broad prospects.Heze City plans to develop oil-use peony as a local key industry.However,there are still many urgent problems that need to be solved for the sustained and healthy devel...Oil-use peony is a treasure with broad prospects.Heze City plans to develop oil-use peony as a local key industry.However,there are still many urgent problems that need to be solved for the sustained and healthy development of the oil-use peony industry.This paper delved into the comprehensive benefits of oil-use peony and its industrial development from the aspects of cultivating high-quality germplasm resources,technological innovation,policy support,developing leading enterprises,improving industrialization system,comprehensive promotion,and strengthening storage and transportation technology,aiming to provide some reference for further development of Heze oil-use peony industry.展开更多
以牡丹籽壳为原料,利用大孔树脂和C18反相键合硅胶柱(C18柱)联合分离短叶松素,回收率为(98.33±0.64)%。用70%(体积分数)乙醇提取牡丹籽壳粗黄酮(Mu Dan Ke Flavonoids,MDKF),得率最高为(10.54±0.13)%;比较了6种大孔树脂(AB-8...以牡丹籽壳为原料,利用大孔树脂和C18反相键合硅胶柱(C18柱)联合分离短叶松素,回收率为(98.33±0.64)%。用70%(体积分数)乙醇提取牡丹籽壳粗黄酮(Mu Dan Ke Flavonoids,MDKF),得率最高为(10.54±0.13)%;比较了6种大孔树脂(AB-8、S-8、DM301、HPD600、HPD100和D101)的吸附率和解析率,发现S-8大孔树脂的吸附率和解析率最佳,分别为83.47%和84.46%;优化S-8大孔树脂分离MDKF的最佳条件为:上样液质量浓度1.6 mg/mL,上样液流速2.0 mL/min,洗脱剂乙醇体积分数60%,洗脱液流速1.5 mL/min,洗脱液体积100 mL;对经过C18柱分离后的组分进行LC-MS分析得到短叶松素,回收率为(98.33±0.64)%;分离前后抗氧化能力比较:C18纯化物>S-8大孔树脂纯化物>MDKF粗提物;分子对接实验表明,短叶松素与超氧化物歧化酶、过氧化氢酶、过氧化物酶、谷胱甘肽过氧化物酶均有结合能力,过氧化氢酶的结合能力最强为-9.1 kcal/mol。实验表明,短叶松素具有较好的抗氧化能力,可作为食品、药品或化妆品等抗氧化剂的添加,应用前景非常广泛,对牡丹籽壳的进一步开发利用提供理论支持。展开更多
基金supported by National Natural Science Foundation of China(Grant Nos.32072614 and 31972452)Shandong Provincial Natural Science Foundation(Grant Nos.ZR2020MC146 and ZR2020QC160)Seed improvement project of Shandong Province(Grant No.2020LZGC011-1-4)。
文摘Tree peony(Paeonia suffruticosa Andrews)is a well-known ornamental plant with high economic value,but the short fluorescence is a key obstacle to its ornamental value and industry development.High temperature accelerates flower senescence and abscission,but the associated mechanisms are poorly understood.In this study,the tandem mass tag(TMT)proteome and label-free quantitative ubiquitome from tree peony cut flowers treated with 20℃for 0 h(RT0),20℃or 28℃for 60 h(RT60 or HT60)were examined based on morphological observation,respectively.Totally,6970 proteins and 1545 lysine ubiquitinated(Kub)sites in 844 proteins were identified.Hydrophilic residues(such as glutamate and aspartate)neighboring the Kub sites were in preference,and 36.01%of the Kub sites were located on the protein surface.The differentially expressed proteins(DEPs)and Kub-DEPs in HT60 vs RT60 were mainly enriched in ribosomal protein,protein biosynthesis,secondary metabolites biosynthesis,flavonoid metabolism,carbohydrate catabolism,and auxin biosynthesis and signaling revealed by GO and KEGG analysis,accompanying the increase of endogenous abscisic acid(ABA)accumulation and decrease of endogenous indoleacetic acid(IAA)level.Additionally,the expression patterns of six enzymes(SAMS,ACO,YUC,CHS,ANS and PFK)putatively with Kub modifications were analyzed by proteome and real-time quantitative RT-PCR.The cell-free degradation assays showed PsSAMS and PsACO proteins could be degraded via the 26 S proteasome system in tree peony flowers.Finally,a working model was proposed for the acceleration of flower senescence and abscission by high temperature.In summary,all results contributed to understanding the mechanism of flower senescence induced by high temperature and prolonging fluorescence in tree peony.
基金supported by grants from the National Natural Science Foundation of China(Grant No.31670663).
文摘The herbaceous peony(Paeonia lactiflora Pall.)has high ornamental value.Replanting problems occur when seedlings are replanted into previous holes.We studied the root system and soil environment of the'Dongjingnvlang'variety under a continuous planting regime of one,four,and seven years,and a replanting regime of one and four years.Under the condition of continuous planting,with the increase of number of years,pH,ammonium nitrogen,and nitrate nitrogen decreased in the rhizosphere and non-rhizosphere soils,whereas organic matter,available phosphorus and potassium,enzyme activities,and the number of bacteria,fungi,and actinomycetes increased.Under the condition of replanting,with the increase of number of years,fungi and actinomycetes in both soils increased,while pH,organic matter,nutrients,enzyme activities,and bacterial number decreased.pH,organic matter,nutrient content,enzyme activity and the number of bacterial were lower in soil replanted for four years,whereas the abundance of fungi and actinomycetes was higher,altering the soil from“bacterial high-fertility”to“fungal low-fertility”with increasing years of replanting.The activity of antioxidant enzymes and MDA content in roots of peony in replanting were higher than those in continuous planting,while the content of osmotic regulatory substances in replanting was lower than that in continuous planting.The results showed that there were no obvious adverse factors in soil during seven years of continuous planting,and herbaceous peony could maintain normal growth and development.However,soils after four years of replanting were not suitable for herbaceous peony growth.Benzoic acid increased with years of replanting,which potentially caused replanting problems.This study provides a theoretical basis for understanding the mechanism of replanting problems in the herbaceous peony.
基金supported by Jiangsu Modern Agricultural Industrial Technology System(JATS[2022]489)Agricultural Science and Technology Independent Innovation Fund of Jiangsu Province(CX(22)3186)+1 种基金Policy Guidance Program of Jiangsu Province-Science and Technology Special Project of Northern Jiangsu Province(SZ-SQ2021041)the Qing Lan Project of Jiangsu Province and High-Level Talent Support Program of Yangzhou University.
文摘Tree peony(Paeonia suffruticosa Andr.)is a traditional Chinese flower,which prefers cool weather.However,high temperature in summer in the middle and lower reaches of the Yangtze River restricts its growth and development.In this study,osmotic regulation,antioxidant enzyme activities,and photosynthetic characteristics of tree peony in response to high-temperature stress were investigated.The results showed that high-temperature stress had destroyed the cell membrane,manifested as the increased relative electrical conductivity and malondialdehyde content.Moreover,high-temperature stress led to excessive accumulation of reactive oxygen species,thereby,activating antioxidant enzyme activities.Also,photosynthetic parameters and chlorophyll fluorescence parameters directly reflected the damage to the photosystem II reflection center under high-temperature stress.In addition,high-temperature stress led to stomatal closure and chloroplast damage.This study revealed the physiological responses of tree peony to high-temperature stress,laying a foundation for the promotion of tree peony in high-temperature areas and the improvement of high-temperature resistance.
基金funded by Jiangsu Agricultural Science and Technology Innovation Fund[CX(20)2030 to J.T.]Qing Lan Project of Jiangsu Province and High-Level Talent Support Program of Yangzhou University.The Key Disciplines of Jiangsu Province.
文摘Herbaceous peony(Paeonia lactiflora Pall.),as a high-end cut flower in the international market,has high ornamental and medicinal values.But in Northern China,drought is a major environmental factor influencing the growth and development of P.lactiflora.Quantitative real-time polymerase chain reaction(qRT-PCR)can evaluate gene expression levels under different stress conditions,and stable internal reference is the key for qRT-PCR.At present,there is no systematic screening of internal reference for correcting gene expressions of P.lactiflora in response to drought stress.In this study,10 candidate genes[ubiquitin(UBQ2),UBQ1,elongation factor 1-α(EF-1α),Histidine(His),eukaryotic initiation factor(eIF),tubulin(TUB),actin(ACT),UBQ3,ACT2,RNA polymerase II(RNA Pol II)]were chosen,and 4 analysis methods were used to compare the stabilities for these 10 genes coping with drought stress.Due to the difference of operation methods,the results of different analysis were distinct,and the final comprehensive analysis indicated that EF-1αwas a relatively stable internal reference gene for P.lactiflora under drought stress.Also,UBQ1 and UBQ2 were the best reference gene combination according to GeNorm analysis.This study will lay a foundation for screening the key genes of P.lactiflora in response to drought stress.
文摘Phoenix dactylifera(Fam:Arecaceae)is originated from the Mesopotamia.The date fruit,was eaten fresh,in various processed forms and dried.The fruits were used traditionally as general tonic,for the treatment of liver diseases,memory disturbances,fever,inflammation,paralysis,loss of consciousness,nervous disorders and consumed by pregnant women before and after delivery.However,all parts of the plant were used for some purpose.Dates fruits were considered a complete diet and a very important item of food,with plenty of vitamins and minerals.It contained a wide range of secondary metabolites.It possessed many pharmacological effects included anticancer,antidiabetic,anti-inflammatory,antimicrobial,antiparasitic,antioxidant,anti-toxin,cardiovascular,hypolipidemic,gastrointestinal,immunomodullatory,neural,hepato and reno-protective,reproductive and wound healing effects.This review highlighted the chemical constituents,nutritional and pharmacological effects of Phoenix dactylifera.
文摘Background: The synergistic mixture of Cyperus esculentus, Phoenix dactylifera and Cocos nucifera (STCD) aqueous extract is a common drink in Port Harcourt, Nigeria. It is assumed to have various health benefits. However, the synergistic mixture of the content has not been studied scientifically, hence the need to evaluate its effect on the liver and kidney being part of the body’s metabolic organs. Aim: This study evaluated the synergistic mixture of Cyperus esculentus, Phoenix dactylifera and Cocos nucifera (STCD) aqueous extract in male albino rats. Methods: Acute toxicity LD<sub>50</sub> of STCD was carried out, afterwards, fifteen male albino rats were grouped into three groups with 5 rats in each group;Control, 200 mg/kg, 400 mg/kg STCD. The rats were administered STCD orally 24 hourly, for 21 days, with feed and water ad libitum. At the end of the experiment, blood samples were collected for biochemical analysis of the liver and kidney biomarkers, while the liver and kidney tissues were harvested for histopathological examination using standard laboratory methods. Descriptive statistics were computed and expressed as Mean ± SD. One-way ANOVA and Turkeys test was performed. P value ≤0.05 was considered statistically significant. Results: Acute toxicity LD50 of STCD was observed to be ≥2404.2 mg/kg body weight. An increase in the percentage body weight difference of 8.39% and 2.86% was observed for 200 and 400 mg/kg STCD groups. Also, the liver weight was observed to increase in 400 mg/kg (3.92 ± 1.42) in comparison to the control group (3.48 ± 1.61), a decrease in the kidney weight was observed in all groups administered STCD in comparison to the control group. Administration of STCD at both 200 and 400 mg/kg revealed a decrease in the concentration of the hepatic biomarkers AST, ALT, ALP, TP, Albumin, Total and conjugated bilirubin. The kidney biomarker Urea was observed to decrease in concentration for 200 mg/kg STCD (4.60 ± 1.83) and 400 mg/kg STCD (4.76 ± 0.74) when compared to the control group (6.32 ± 2.74). A decrease in Creatinine was observed in 200 mg/kg (91.80 ± 34.69) and 400 mg/kg (98.60 ± 15.53) in comparison to the control group (117.60 ± 42.88). The histological examination of the liver of rats administered STCD revealed structural normal central vein, hepatocytes and portal tract. The kidney examination revealed normal glomeruli and normal tubule. Conclusion: The findings of this study opine that STCD improved the health of both the liver and kidney as evidenced via the biomarkers and histological examinations of the liver and kidney. This study therefore recommends the intake of STCD at moderate doses for improved liver and kidney function due to its bioactive compounds and nutritional content.
基金Supported by Social Science Planning Project of Heze City in 2022(HSKGH[2022]3)。
文摘The peony industry is a characteristic industry in Heze City,and oil-use peony is an emerging industry.Heze City has planned oil-use peony as a key support and development industry in the local area.The oil-use peony industry is full of treasures,but there are still many urgent problems to be solved for its sustained and healthy development:cultivation and planting,variety selection,product processing,and enterprise development,which have hindered the development of the oil-use peony industry.This paper delved into the comprehensive benefits of oil-use peony and its industrial development,providing some reference for the further development of the oil-use peony industry in Heze.
基金Supported by Social Science Planning Project of Heze City in 2022 (HSKGH[2022]3)Key Laboratory of Plant Biology,Heze University.
文摘Oil-use peony is a treasure with broad prospects.Heze City plans to develop oil-use peony as a local key industry.However,there are still many urgent problems that need to be solved for the sustained and healthy development of the oil-use peony industry.This paper delved into the comprehensive benefits of oil-use peony and its industrial development from the aspects of cultivating high-quality germplasm resources,technological innovation,policy support,developing leading enterprises,improving industrialization system,comprehensive promotion,and strengthening storage and transportation technology,aiming to provide some reference for further development of Heze oil-use peony industry.
文摘以牡丹籽壳为原料,利用大孔树脂和C18反相键合硅胶柱(C18柱)联合分离短叶松素,回收率为(98.33±0.64)%。用70%(体积分数)乙醇提取牡丹籽壳粗黄酮(Mu Dan Ke Flavonoids,MDKF),得率最高为(10.54±0.13)%;比较了6种大孔树脂(AB-8、S-8、DM301、HPD600、HPD100和D101)的吸附率和解析率,发现S-8大孔树脂的吸附率和解析率最佳,分别为83.47%和84.46%;优化S-8大孔树脂分离MDKF的最佳条件为:上样液质量浓度1.6 mg/mL,上样液流速2.0 mL/min,洗脱剂乙醇体积分数60%,洗脱液流速1.5 mL/min,洗脱液体积100 mL;对经过C18柱分离后的组分进行LC-MS分析得到短叶松素,回收率为(98.33±0.64)%;分离前后抗氧化能力比较:C18纯化物>S-8大孔树脂纯化物>MDKF粗提物;分子对接实验表明,短叶松素与超氧化物歧化酶、过氧化氢酶、过氧化物酶、谷胱甘肽过氧化物酶均有结合能力,过氧化氢酶的结合能力最强为-9.1 kcal/mol。实验表明,短叶松素具有较好的抗氧化能力,可作为食品、药品或化妆品等抗氧化剂的添加,应用前景非常广泛,对牡丹籽壳的进一步开发利用提供理论支持。