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Preparation for CSST:Star-galaxy Classification using a Rotationally Invariant Supervised Machine Learning Method
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作者 Shiliang Zhang Guanwen Fang +6 位作者 Jie Song Ran Li Yizhou Gu Zesen Lin Chichun Zhou Yao Dai Xu Kong 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2024年第9期136-146,共11页
Most existing star-galaxy classifiers depend on the reduced information from catalogs,necessitating careful data processing and feature extraction.In this study,we employ a supervised machine learning method(GoogLeNet... Most existing star-galaxy classifiers depend on the reduced information from catalogs,necessitating careful data processing and feature extraction.In this study,we employ a supervised machine learning method(GoogLeNet)to automatically classify stars and galaxies in the COSMOS field.Unlike traditional machine learning methods,we introduce several preprocessing techniques,including noise reduction and the unwrapping of denoised images in polar coordinates,applied to our carefully selected samples of stars and galaxies.By dividing the selected samples into training and validation sets in an 8:2 ratio,we evaluate the performance of the GoogLeNet model in distinguishing between stars and galaxies.The results indicate that the GoogLeNet model is highly effective,achieving accuracies of 99.6% and 99.9% for stars and galaxies,respectively.Furthermore,by comparing the results with and without preprocessing,we find that preprocessing can significantly improve classification accuracy(by approximately 2.0% to 6.0%)when the images are rotated.In preparation for the future launch of the China Space Station Telescope(CSST),we also evaluate the performance of the GoogLeNet model on the CSST simulation data.These results demonstrate a high level of accuracy(approximately 99.8%),indicating that this model can be effectively utilized for future observations with the CSST. 展开更多
关键词 methods:data analysis techniques:image processing stars:imaging
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Analysis of the Cicatricial Acceleration Method (MAC®) in Skin Repair in Wistar Rattus norvegicus with Induced Chemical Burns
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作者 Marcus Vinicius de Mello Pinto Sandroval Francisco Torres +9 位作者 Aline Ronis Sampaio Karin Yuri Fernandez Iturra Miriam Viviane Baron Patricia Froes Carlos Ruiz da Silva Daiane Paza Maria Elena Silva Alvarez Josefa Jeanette Ugalde Juliano Tibola Esteban Fortuny 《Journal of Biosciences and Medicines》 2024年第9期204-214,共11页
Introduction: The cicatricial acceleration method (MAC®) promotes photobiological effects of an anti-inflammatory and healing nature. Its therapeutic radiation is emitted, producing photobiostimulant effects that... Introduction: The cicatricial acceleration method (MAC®) promotes photobiological effects of an anti-inflammatory and healing nature. Its therapeutic radiation is emitted, producing photobiostimulant effects that result in rapid tissue repair and better tissue quality. The treatment of burns has always been a challenge, which involves both performing surgery and controlling and guiding scar regeneration, avoiding possible morbidities. Objective: To evaluate the effects of applying the MAC methodology with an AlGa (aluminum, gallium arsenide) laser on the time and quality of tissue repair in the skin of rats after induced chemical burns. Method: 22 adult male rats were subjected to a second-degree chemical burn on the back using 50% trichloroacetic acid. After the burns, the animals were randomly separated into 2 groups: control and experimental. The control group (G1) received placebo laser therapy and the laser group (G2) underwent laser irradiation with an energy density of 100 J/cm2. Histological analysis and macroscopic evaluation were carried out by means of the paper template method. Results: Group G1 showed (53%) of the necrosis area and group G2 showed (11%) necrosis area. Conclusion: The cicatricial acceleration method (MAC®) favored the repair of wounds caused by a 2nd-degree chemical burn, optimizing time and improving quality. 展开更多
关键词 Chemical Burn HEALING SCARRING Cicatricial Acceleration method (MAC®) Tissue Repair
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Lone-Star retractor perineal exposure method for laparoscopic abdominal perineal resection of rectal cancer
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作者 Jun Ma Dai-Bin Tang +3 位作者 Yu-Quan Tang Da-Tian Wang Peng Jiang Ya-Ming Zhang 《World Journal of Gastrointestinal Surgery》 SCIE 2024年第8期2528-2537,共10页
BACKGROUND The abdominal perineal resection(APR),historically referred to as Mile’s proce-dure,stands as a time-honored surgical intervention for rectal cancer manage-ment.Advancements in surgical techniques and the ... BACKGROUND The abdominal perineal resection(APR),historically referred to as Mile’s proce-dure,stands as a time-honored surgical intervention for rectal cancer manage-ment.Advancements in surgical techniques and the advent of neoadjuvant therapies have significantly improved the rate of sphincter preservation among patients afflicted with rectal cancer,including those with ultralow rectal cancer.Despite these improvements,APR maintains its irreplaceable role in the clinical landscape,particularly for cases involving low rectal cancer with encroachment on the external anal sphincter or levator ani muscles.Optimal perineal exposure stands as a pivotal phase in APR,given that the precision of this maneuver is directly correlated with both the safety of the surgery and the patient’s subse-quent long-term prognosis.AIM To evaluate the value of Lone-Star retractor(LSR)perineal exposure method in the treatment for laparoscopic APR of rectal cancer.METHODS We reviewed the records of 38 patients with rectal cancer at Anqing Municipal Hospital from January 2020 to December 2023,including 20 patients who underwent the APR procedure with a LSR perineal exposure method(LSR group)and 18 patients who underwent the APR procedure with a conventional perineal exposure method(control group).In the LSR group,following incision of the skin and subcutaneous tissue,the LSR was placed and dynamically adjusted according to the surgical plane to fully expose the perineal operative field.RESULTS A total of 38 patients underwent laparoscopic APR,none of whom were found to have distant metastasis upon intraoperative exploration.Perineal blood loss,the postoperative hospital stays and the wound pain scores in the LSR group were significantly lower than those in the control group.A single surgeon completed the perineal operation significantly more often in the LSR group than in the control group(P<0.05).The incidence of infection via the perineal incision in the LSR group was significantly lower than that in the control group(P<0.05).No cases of distant metastasis or local recurrence were found among the patients at the postoperative follow-up.CONCLUSION The application of the LSR technique might be helpful for performing perineal exposure during APR for rectal cancer and could reduce the incidence of perineal complications,shorten the postoperative hospital stay,improve postoperative pain,and allow one surgeon to perform the perineal operation. 展开更多
关键词 Rectal cancer Lone-star retractor Abdominal perineal resection Perineal exposure method Technique
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An Adaptive Spectral Conjugate Gradient Method with Restart Strategy
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作者 Zhou Jincheng Jiang Meixuan +2 位作者 Zhong Zining Wu Yanqiang Shao Hu 《数学理论与应用》 2024年第3期106-118,共13页
As a generalization of the two-term conjugate gradient method(CGM),the spectral CGM is one of the effective methods for solving unconstrained optimization.In this paper,we enhance the JJSL conjugate parameter,initiall... As a generalization of the two-term conjugate gradient method(CGM),the spectral CGM is one of the effective methods for solving unconstrained optimization.In this paper,we enhance the JJSL conjugate parameter,initially proposed by Jiang et al.(Computational and Applied Mathematics,2021,40:174),through the utilization of a convex combination technique.And this improvement allows for an adaptive search direction by integrating a newly constructed spectral gradient-type restart strategy.Then,we develop a new spectral CGM by employing an inexact line search to determine the step size.With the application of the weak Wolfe line search,we establish the sufficient descent property of the proposed search direction.Moreover,under general assumptions,including the employment of the strong Wolfe line search for step size calculation,we demonstrate the global convergence of our new algorithm.Finally,the given unconstrained optimization test results show that the new algorithm is effective. 展开更多
关键词 Unconstrained optimization Spectral conjugate gradient method Restart strategy Inexact line search Global convergence
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骨科机器人联合Starr骨盆复位架治疗Tile C型骨盆环骨折 被引量:1
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作者 姜刚强 焦福德 +3 位作者 应霁翀 虞天明 刘建磊 庄云强 《中国骨伤》 CAS CSCD 2024年第5期445-450,共6页
目的:探讨骨科机器人联合Starr骨盆复位架治疗Tile C型骨盆环骨折的临床疗效。方法:2019年10月至2021年5月采用机器人联合Starr骨盆复位架治疗14例Tile C型骨盆环骨折患者,男9例,女5例;年龄33~69岁。14例患者均为新鲜闭合骨折且不合并... 目的:探讨骨科机器人联合Starr骨盆复位架治疗Tile C型骨盆环骨折的临床疗效。方法:2019年10月至2021年5月采用机器人联合Starr骨盆复位架治疗14例Tile C型骨盆环骨折患者,男9例,女5例;年龄33~69岁。14例患者均为新鲜闭合骨折且不合并股骨、胫腓骨骨折等损伤。入院后4~7 d完成手术,术中在可透视碳素床,通过Starr骨盆复位架牵引复位骨盆环,联合骨科机器人治疗C型骨盆环骨折。观察手术时间、出血量、单枚螺钉置入透视次数、骨折复位质量、患肢功能及并发症等。采用Matta评分标准进行放射学复位评价,末次随访采用Majeed骨盆功能评分系统进行临床疗效评价。结果:14例患者均顺利完成手术,手术时间84~141 min,手术出血量20~50 ml,单枚螺钉置入透视次数4~9次。14例患者均获得随访,随访时间12~24个月。术后所有骨折获得骨性愈合,愈合时间3~7个月,未发现内固定断裂、螺钉松动、感染、神经损伤等并发症。依据Matta影像学复位评估标准:优9例,良4例,可1例。末次随访采用Majeed骨盆功能评分系统:优10例,良4例。结论:机器人联合Starr骨盆复位架治疗C型骨盆环新鲜骨折操作简单、缩短了手术和麻醉时间、并发症少,降低了手术风险,实现了骨盆骨折的微创治疗。但对于陈旧性骨折,因骨折复位困难,该技术并不适用。对于螺钉进钉点区域粉碎的骨折,通道螺钉置入困难,建议采用其他方式固定。对于伴有骨盆外伤史或骨盆发育异常者,术前需仔细评估有无经皮螺钉通道,避免损伤神经、血管。 展开更多
关键词 骨科机器人 starr骨盆复位架 骨盆骨折 螺钉 内固定
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日本沼虾StAR基因克隆及其低氧胁迫下表达分析 被引量:1
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作者 郑诚 薛程 +1 位作者 赵倩倩 孙盛明 《水产学报》 CAS CSCD 北大核心 2024年第3期64-75,共12页
为了研究类固醇激素合成急性调节蛋白(steroidogenic acute regulatory protein, StAR)在日本沼虾应答低氧胁迫过程中所发挥的作用,实验应用c DNA末端快速扩增(RACE)PCR技术克隆了日本沼虾StAR全长cDNA序列,并利用在线软件对其序列特征... 为了研究类固醇激素合成急性调节蛋白(steroidogenic acute regulatory protein, StAR)在日本沼虾应答低氧胁迫过程中所发挥的作用,实验应用c DNA末端快速扩增(RACE)PCR技术克隆了日本沼虾StAR全长cDNA序列,并利用在线软件对其序列特征进行生物信息学分析,采用半定量RT-PCR方法分析StAR在日本沼虾不同组织的分布情况,采用实时荧光定量PCR(qRT-PCR)与免疫印迹(Western blot)技术对其在不同低氧胁迫阶段中的表达规律进行检测,观察低氧胁迫下精巢组织中StAR蛋白免疫组化定位。结果显示,日本沼虾StAR其cDNA全长3 361 bp (NCBI登录号:MW263908),包括5′-UTR 323 bp,3′-UTR 2 129 bp,开放阅读框909 bp,编码302个氨基酸。氨基酸序列比对及系统进化分析结果显示,日本沼虾StAR基因与三疣梭子蟹等甲壳动物StAR聚类一支,具有最近的亲缘关系。半定量RT-PCR检测结果显示,StAR在日本沼虾不同组织中均有表达,其表达量在肝胰腺和心脏组织中最低,在精巢组织中表达处于较高水平。使用qRT-PCR技术检测日本沼虾精巢中StAR在低氧胁迫下时空表达情况,结果表明,与对照组相比,低氧处理组精巢中StAR基因表达量在1~48 h均显著上调,而在低氧处理组96 h显著降低。此外,本实验对StAR进行了原核表达及抗血清制备,采用Western blot检测StAR蛋白表达丰度基本与基因表达模式相似,免疫组化结果显示,StAR蛋白在精细胞与间质细胞中均有表达。综上所述,长期低氧胁迫显著降低了StAR基因的转录表达水平,并且该基因可能在类固醇合成路径及精子发生过程中均发挥重要作用。本研究结果为进一步解析日本沼虾类固醇激素合成分子机制奠定基础。 展开更多
关键词 日本沼虾 star 克隆 低氧 表达分析
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STAR基因与东湖杂交奶绵羊繁殖性状的关联分析 被引量:1
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作者 李桂丽 李丹妮 +6 位作者 王丽 吴卓芯 王钰晨 郭磊 张希云 张磊 宋宇轩 《中国畜牧杂志》 CAS CSCD 北大核心 2024年第1期137-142,共6页
本实验旨在探究类固醇激素合成急性调节蛋白(Steroidogenic Acute Regulatory Protein,STAR)基因多态性与东湖杂一代奶绵羊发情鉴定评分、产羔数的相关性,鉴定出影响绵羊繁殖性能的关键性位点。本研究以339只东湖杂一代奶绵羊为研究对象... 本实验旨在探究类固醇激素合成急性调节蛋白(Steroidogenic Acute Regulatory Protein,STAR)基因多态性与东湖杂一代奶绵羊发情鉴定评分、产羔数的相关性,鉴定出影响绵羊繁殖性能的关键性位点。本研究以339只东湖杂一代奶绵羊为研究对象,采用竞争性等位基因特异性PCR(KASP)技术对STAR基因的g.31959747T>C、g.31959875A>C2个位点进行基因分型,并利用GLM模型对SNP位点与发情鉴定评分和产羔数进行关联分析。结果表明,g.31959747T>C与奶绵羊发情鉴定评分呈极显著相关,TT型的发情表现更优,但与产羔数差异不显著;g.31959875A>C与发情评分和产羔数均无显著关系;聚合基因型与繁殖性状的关联分析结果表明,TCAC型个体在发情评分、平均产羔数方面均较低。综上,STAR基因g.31959747T>C位点是影响奶绵羊发情评分的关键性位点,可根据育种目标将g.31959747T>C位点作为提高奶绵羊同期发情效果的分子标记,同时将TCAC聚合基因型及时淘汰。本研究结果可为奶绵羊繁殖性状的选育提供参考。 展开更多
关键词 star 东湖杂交奶绵羊 关联分析 发情鉴定评分 产羔数
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STAR基因在不同发育期绵羊睾丸和附睾中的表达与组织分布
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作者 蒋雨婷 王霞 +5 位作者 宋娟娟 李晨硕 王彩丽 陈浩林 李讨讨 马友记 《中国畜禽种业》 2024年第4期77-84,1,共9页
为探讨STAR基因在发育的绵羊睾丸和附睾中的表达模式及潜在功能,该研究采集3月龄、 1周岁和3周岁藏绵羊睾丸和附睾(头、体和尾)组织样本,利用实时荧光定量PCR、蛋白免疫印迹和免疫组织荧光染色法检测STAR基因的表达及其编码蛋白的细胞... 为探讨STAR基因在发育的绵羊睾丸和附睾中的表达模式及潜在功能,该研究采集3月龄、 1周岁和3周岁藏绵羊睾丸和附睾(头、体和尾)组织样本,利用实时荧光定量PCR、蛋白免疫印迹和免疫组织荧光染色法检测STAR基因的表达及其编码蛋白的细胞定位。结果发现,随着年龄的增加,在藏绵羊睾丸和附睾(附睾头除外)中STAR基因在转录水平的表达总体呈下降趋势,而在蛋白质水平的表达呈上调趋势。STAR蛋白主要存在于藏绵羊睾丸间质细胞和附睾假复层纤毛上皮主细胞。鉴于间质细胞和主细胞的主要生物学功能是分泌睾酮和分泌促进精子成熟所需的蛋白质,由此推测,STAR基因可能主要在睾丸间质细胞和附睾主细胞的发育和功能维持中发挥作用,进而促进雄激素的合成和精子的成熟。 展开更多
关键词 绵羊 star基因 睾丸 附睾
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A Review on Sources,Extractions and Analysis Methods of a Sustainable Biomaterial:Tannins 被引量:2
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作者 Antonio Pizzi Marie-Pierre Laborie Zeki Candan 《Journal of Renewable Materials》 EI CAS 2024年第3期397-425,共29页
Condensed and hydrolysable tannins are non-toxic natural polyphenols that are a commercial commodity industrialized for tanning hides to obtain leather and for a growing number of other industrial applications mainly ... Condensed and hydrolysable tannins are non-toxic natural polyphenols that are a commercial commodity industrialized for tanning hides to obtain leather and for a growing number of other industrial applications mainly to substitute petroleum-based products.They are a definite class of sustainable materials of the forestry industry.They have been in operation for hundreds of years to manufacture leather and now for a growing number of applications in a variety of other industries,such as wood adhesives,metal coating,pharmaceutical/medical applications and several others.This review presents the main sources,either already or potentially commercial of this forestry by-materials,their industrial and laboratory extraction systems,their systems of analysis with their advantages and drawbacks,be these methods so simple to even appear primitive but nonetheless of proven effectiveness,or very modern and instrumental.It constitutes a basic but essential summary of what is necessary to know of these sustainable materials.In doing so,the review highlights some of the main challenges that remain to be addressed to deliver the quality and economics of tannin supply necessary to fulfill the industrial production requirements for some materials-based uses. 展开更多
关键词 TANNINS FLAVONOIDS SOURCES extraction methods analysis methods
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Free Energy of Anisotropic Strangeon Stars 被引量:1
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作者 Shichuan Chen Yong Gao +1 位作者 Enping Zhou Renxin Xu 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2024年第2期50-57,共8页
Can pulsar-like compact objects release further huge free energy besides the kinematic energy of rotation?This is actually relevant to the equation of state of cold supra-nuclear matter,which is still under hot debate... Can pulsar-like compact objects release further huge free energy besides the kinematic energy of rotation?This is actually relevant to the equation of state of cold supra-nuclear matter,which is still under hot debate.Enormous energy is surely needed to understand various observations,such asγ-ray bursts,fast radio bursts and softγ-ray repeaters.In this paper,the elastic/gravitational free energy of solid strangeon stars is revisited for strangeon stars,with two anisotropic models to calculate in general relativity.It is found that huge free energy(>10^(46)erg)could be released via starquakes,given an extremely small anisotropy((p_(t)-p_(r))/p_(r)~10^(-4),with pt/pr the tangential/radial pressure),implying that pulsar-like stars could have great potential of free energy release without extremely strong magnetic fields in the solid strangeon star model. 展开更多
关键词 (stars:)pulsars general-methods numerical-(stars:)gamma-ray bursts general
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Drilling-based measuring method for the c-φ parameter of rock and its field application 被引量:3
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作者 Bei Jiang Fenglin Ma +5 位作者 Qi Wang Hongke Gao Dahu Zhai Yusong Deng Chuanjie Xu Liangdi Yao 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第1期65-76,共12页
The technology of drilling tests makes it possible to obtain the strength parameter of rock accurately in situ. In this paper, a new rock cutting analysis model that considers the influence of the rock crushing zone(R... The technology of drilling tests makes it possible to obtain the strength parameter of rock accurately in situ. In this paper, a new rock cutting analysis model that considers the influence of the rock crushing zone(RCZ) is built. The formula for an ultimate cutting force is established based on the limit equilibrium principle. The relationship between digital drilling parameters(DDP) and the c-φ parameter(DDP-cφ formula, where c refers to the cohesion and φ refers to the internal friction angle) is derived, and the response of drilling parameters and cutting ratio to the strength parameters is analyzed. The drillingbased measuring method for the c-φ parameter of rock is constructed. The laboratory verification test is then completed, and the difference in results between the drilling test and the compression test is less than 6%. On this basis, in-situ rock drilling tests in a traffic tunnel and a coal mine roadway are carried out, and the strength parameters of the surrounding rock are effectively tested. The average difference ratio of the results is less than 11%, which verifies the effectiveness of the proposed method for obtaining the strength parameters based on digital drilling. This study provides methodological support for field testing of rock strength parameters. 展开更多
关键词 Digital drilling Rock crushing zone c-u parameter Measurement method Field application
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Efficient slope reliability and sensitivity analysis using quantile-based first-order second-moment method 被引量:1
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作者 Zhiyong Yang Chengchuan Yin +2 位作者 Xueyou Li Shuihua Jiang Dianqing Li 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第10期4192-4203,共12页
This paper introduces a novel approach for parameter sensitivity evaluation and efficient slope reliability analysis based on quantile-based first-order second-moment method(QFOSM).The core principles of the QFOSM are... This paper introduces a novel approach for parameter sensitivity evaluation and efficient slope reliability analysis based on quantile-based first-order second-moment method(QFOSM).The core principles of the QFOSM are elucidated geometrically from the perspective of expanding ellipsoids.Based on this geometric interpretation,the QFOSM is further extended to estimate sensitivity indices and assess the significance of various uncertain parameters involved in the slope system.The proposed method has the advantage of computational simplicity,akin to the conventional first-order second-moment method(FOSM),while providing estimation accuracy close to that of the first-order reliability method(FORM).Its performance is demonstrated with a numerical example and three slope examples.The results show that the proposed method can efficiently estimate the slope reliability and simultaneously evaluate the sensitivity of the uncertain parameters.The proposed method does not involve complex optimization or iteration required by the FORM.It can provide a valuable complement to the existing approximate reliability analysis methods,offering rapid sensitivity evaluation and slope reliability analysis. 展开更多
关键词 Slope reliability Sensitivity analysis QUANTILE First-order second-moment method(FOSM) First-order reliability method(FORM)
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A comparison study on structure-function relationship of polysaccharides obtained from sea buckthorn berries using different methods:antioxidant and bile acid-binding capacity 被引量:5
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作者 Qiaoyun Li Zuman Dou +5 位作者 Qingfei Duan Chun Chen Ruihai Liu Yueming Jiang Bao Yang Xiong Fu 《Food Science and Human Wellness》 SCIE CSCD 2024年第1期494-505,共12页
In this study,the structural characters,antioxidant activities and bile acid-binding ability of sea buckthorn polysaccharides(HRPs)obtained by the commonly used hot water(HRP-W),pressurized hot water(HRP-H),ultrasonic... In this study,the structural characters,antioxidant activities and bile acid-binding ability of sea buckthorn polysaccharides(HRPs)obtained by the commonly used hot water(HRP-W),pressurized hot water(HRP-H),ultrasonic(HRP-U),acid(HRP-C)and alkali(HRP-A)assisted extraction methods were investigated.The results demonstrated that extraction methods had significant effects on extraction yield,monosaccharide composition,molecular weight,particle size,triple-helical structure,and surface morphology of HRPs except for the major linkage bands.Thermogravimetric analysis showed that HRP-U with filamentous reticular microstructure exhibited better thermal stability.The HRP-A with the lowest molecular weight and highest arabinose content possessed the best antioxidant activities.Moreover,the rheological analysis indicated that HRPs with higher galacturonic acid content and molecular weight showed higher viscosity and stronger crosslinking network(HRP-C,HRP-W and HRP-U),which exhibited stronger bile acid binding capacity.The present findings provide scientific evidence in the preparation technology of sea buckthorn polysaccharides with good antioxidant and bile acid binding capacity which are related to the structure affected by the extraction methods. 展开更多
关键词 Sea buckthorn Extraction method STRUCTURE Rheological properties Antioxidant activity Bile acid binding capacity
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靶向小鼠Star基因的CRISPR/Cas9系统构建与打靶效力评价
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作者 韦妙灵 韦柳青 +3 位作者 杨惠元 牧苏婉 李夏 李勇 《华夏医学》 CAS 2024年第5期29-35,共7页
目的利用CRISPR/Cas9系统对小鼠Star基因进行精确编辑,构建模拟人类STAR基因突变的细胞模型。方法选择中国先天性类脂质性肾上腺皮质增生症(CLAH)患者的热点突变区域,并设计相应的单导向RNA(sgRNA)。构建表达sgRNA的重组质粒,将其与慢... 目的利用CRISPR/Cas9系统对小鼠Star基因进行精确编辑,构建模拟人类STAR基因突变的细胞模型。方法选择中国先天性类脂质性肾上腺皮质增生症(CLAH)患者的热点突变区域,并设计相应的单导向RNA(sgRNA)。构建表达sgRNA的重组质粒,将其与慢病毒包装质粒共同转染至HEK-293T细胞并制备慢病毒颗粒以感染小鼠TCMK细胞。采用嘌呤霉素进行病毒感染后阳性细胞的筛选,提取阳性细胞的基因组DNA,通过聚合酶链式反应(PCR)扩增目标位点的基因组DNA片段,并利用TA克隆和Sanger测序技术,对编辑位点与效率进行统计分析。结果成功设计了有效的sgRNA序列,并利用CRISPR/Cas9系统成功构建了编辑小鼠Star基因的细胞模型。结论本研究通过精确编辑小鼠Star基因,成功构建了模拟人类STAR基因突变的CRISPR/Cas9系统,为深入研究CLAH的致病机制及探索潜在治疗方法提供有力工具,具有重要的科学意义和应用价值。 展开更多
关键词 基因编辑 CRISPR/Cas9 单导向RNA 小鼠star基因 先天性类脂质性肾上腺皮质增生症
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Material point method simulation of hydro-mechanical behaviour in twophase porous geomaterials: A state-of-the-art review 被引量:2
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作者 Xiangcou Zheng Shuying Wang +1 位作者 Feng Yang Junsheng Yang 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第6期2341-2350,共10页
The material point method(MPM)has been gaining increasing popularity as an appropriate approach to the solution of coupled hydro-mechanical problems involving large deformation.In this paper,we survey the current stat... The material point method(MPM)has been gaining increasing popularity as an appropriate approach to the solution of coupled hydro-mechanical problems involving large deformation.In this paper,we survey the current state-of-the-art in the MPM simulation of hydro-mechanical behaviour in two-phase porous geomaterials.The review covers the recent advances and developments in the MPM and their extensions to capture the coupled hydro-mechanical problems involving large deformations.The focus of this review is aiming at providing a clear picture of what has or has not been developed or implemented for simulating two-phase coupled large deformation problems,which will provide some direct reference for both practitioners and researchers. 展开更多
关键词 Coupled problems Hydro-mechanical behaviour Large deformation Material Point method(MPM)
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Volumetric lattice Boltzmann method for pore-scale mass diffusionadvection process in geopolymer porous structures 被引量:1
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作者 Xiaoyu Zhang Zirui Mao +6 位作者 Floyd W.Hilty Yulan Li Agnes Grandjean Robert Montgomery Hans-Conrad zur Loye Huidan Yu Shenyang Hu 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第6期2126-2136,共11页
Porous materials present significant advantages for absorbing radioactive isotopes in nuclear waste streams.To improve absorption efficiency in nuclear waste treatment,a thorough understanding of the diffusion-advecti... Porous materials present significant advantages for absorbing radioactive isotopes in nuclear waste streams.To improve absorption efficiency in nuclear waste treatment,a thorough understanding of the diffusion-advection process within porous structures is essential for material design.In this study,we present advancements in the volumetric lattice Boltzmann method(VLBM)for modeling and simulating pore-scale diffusion-advection of radioactive isotopes within geopolymer porous structures.These structures are created using the phase field method(PFM)to precisely control pore architectures.In our VLBM approach,we introduce a concentration field of an isotope seamlessly coupled with the velocity field and solve it by the time evolution of its particle population function.To address the computational intensity inherent in the coupled lattice Boltzmann equations for velocity and concentration fields,we implement graphics processing unit(GPU)parallelization.Validation of the developed model involves examining the flow and diffusion fields in porous structures.Remarkably,good agreement is observed for both the velocity field from VLBM and multiphysics object-oriented simulation environment(MOOSE),and the concentration field from VLBM and the finite difference method(FDM).Furthermore,we investigate the effects of background flow,species diffusivity,and porosity on the diffusion-advection behavior by varying the background flow velocity,diffusion coefficient,and pore volume fraction,respectively.Notably,all three parameters exert an influence on the diffusion-advection process.Increased background flow and diffusivity markedly accelerate the process due to increased advection intensity and enhanced diffusion capability,respectively.Conversely,increasing the porosity has a less significant effect,causing a slight slowdown of the diffusion-advection process due to the expanded pore volume.This comprehensive parametric study provides valuable insights into the kinetics of isotope uptake in porous structures,facilitating the development of porous materials for nuclear waste treatment applications. 展开更多
关键词 Volumetric lattice Boltzmann method(VLBM) Phase field method(PFM) Pore-scale diffusion-advection Nuclear waste treatment Porous media flow Graphics processing unit(GPU) parallelization
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The Completeness of Accreting Neutron Star Binary Candidates from the Chinese Space Station Telescope
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作者 Hao Shen Shun-Yi Lan Xiang-Cun Meng 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2024年第9期28-36,共9页
A neutron star(NS)has many extreme physical conditions,and one may obtain some important information about an NS via accreting neutron star binary(ANSB)systems.The upcoming Chinese Space Station Telescope(CSST)provide... A neutron star(NS)has many extreme physical conditions,and one may obtain some important information about an NS via accreting neutron star binary(ANSB)systems.The upcoming Chinese Space Station Telescope(CSST)provides an opportunity to search for a large sample of ANSB candidates.Our goal is to check the completeness of the potential ANSB samples from CSST data.In this paper,we generate some ANSBs and normal binaries under the CSST photometric system by binary evolution and binary population synthesis method and use a machine learning method to train a classification model.Although the Precision(94.56%)of our machine learning model is as high as before study,the Recall is only about 63.29%.The Precision/Recall is mainly determined by the mass transfer rate between the NSs and their companions.In addition,we also find that the completeness of ANSB samples from CSST photometric data by the machine learning method also depends on the companion mass and the age of the system.ANSB candidates with a low initial mass companion star(0.1 M_(⊙)to 1 M_(⊙))have a relatively high Precision(94.94%)and high Recall(86.32%),whereas ANSB candidates with a higher initial mass companion star(1.1 M_(⊙)to 3 M_(⊙))have similar Precision(93.88%)and quite low Recall(42.67%).Our results indicate that although the machine learning method may obtain a relatively pure sample of ANSBs,a completeness correction is necessary for one to obtain a complete sample. 展开更多
关键词 stars:neutron X-rays:binaries methods:analytical
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A Localization Method of High Energy Transients for All-sky Gamma-ray Monitor
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作者 Yi Zhao Wangchen Xue +62 位作者 Shaolin Xiong Qi Luo Yuanhao Wang Jiacong Liu Heng Yu Xiaoyun Zhao Yue Huang Jinyuan Liao Jianchao Sun Xiaobo Li Qibin Yi Ce Cai Shuo Xiao Shenglun Xie Chao Zheng Yanqiu Zhang Chenwei Wang Wenjun Tan Zhiwei Guo Chaoyang Li Zhenghua An Gang Chen Yanqi Du Min Gao Ke Gong Dongya Guo Jiang He Jianjian He Bing Li Gang Li Xinqiao Li Jing Liang Xiaohua Liang Yaqing Liu Xiang Ma Rui Qiao Liming Song Xinying Song Xilei Sun Jin Wang Ping Wang Xiangyang Wen Hong Wu Yanbing Xu Sheng Yang Dali Zhang Fan Zhang Hongmei Zhang Peng Zhang Shu Zhang Zhen Zhang Shijie Zheng Keke Zhang Xingbo Han Haiyan Wu Hu Tai Hao Geng Gaopeng Lu Wei Xu Fanchao Lyu Hongbo Zhang Fangjun Lu Shuangnan Zhang 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2024年第10期25-35,共11页
Fast and reliable localization of high-energy transients is crucial for characterizing the burst properties and guiding the follow-up observations.Localization based on the relative counts of different detectors has b... Fast and reliable localization of high-energy transients is crucial for characterizing the burst properties and guiding the follow-up observations.Localization based on the relative counts of different detectors has been widely used for all-sky gamma-ray monitors.There are two major methods for this count distribution localization:χ^(2)minimization method and the Bayesian method.Here we propose a modified Bayesian method that could take advantage of both the accuracy of the Bayesian method and the simplicity of the χ^(2)method.With comprehensive simulations,we find that our Bayesian method with Poisson likelihood is generally more applicable for various bursts than the χ^(2)method,especially for weak bursts.We further proposed a location-spectrum iteration approach based on the Bayesian inference,which could alleviate the problems caused by the spectral difference between the burst and location templates.Our method is very suitable for scenarios with limited computation resources or timesensitive applications,such as in-flight localization software,and low-latency localization for rapidly follow-up observations. 展开更多
关键词 methods:data analysis methods:analytical (stars:)gamma-ray burst:general
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The EOSs and the Blatant Discrepancy in Modelling Massive Neutron Stars: Origin and a Possible Solution Method
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作者 Ahmad A. Hujeirat Mauritz M. Wicker 《Journal of Modern Physics》 2023年第11期1458-1463,共6页
Exploring the state of ultra-cold supranuclear dense matter that makes up the cores of massive neutron stars is one of the greatest unresolved problems in modern physics. In this letter, we show that when the interior... Exploring the state of ultra-cold supranuclear dense matter that makes up the cores of massive neutron stars is one of the greatest unresolved problems in modern physics. In this letter, we show that when the interiors of pulsars are made of compressible and dissipative normal matter, the commonly used solution procedures combined with the known EOSs yield widely scattered solutions and poorly determined radii. A remarkable agreement emerges, however, if pulsars harbour cores that are made of incompressible entropy-free superfluids (SuSu-matter) embedded in flat spacetimes. Such supranuclear dense matter should condensate to form false vacua as predicated by non-perterbative QCD vacuum. The solutions here are found to be physically consistent and mathematically elegant, irrespective of the object’s mass. Based thereon, we conclude that the true masses of massive NSs may differ significantly from those revealed by direct observation. 展开更多
关键词 General Relativity PULSARS Neutron stars EOSs QCD INCOMPRESSIBILITY SUPERFLUIDITY Super-Conductivity
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Possible Habitats for NH_(3),NH_(2)D,H^(13)CN,HC^(15)N,SO,and C^(18)O in the Initial Conditions of High-mass Star Formation
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作者 Quan-Ling Cui Chuan-Peng Zhang Jun-Jie Wang 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2024年第4期237-252,共16页
The initial condition of high-mass star formation is a complex area of study because of the high densities(n_(H_(2))>106cm^(-3))and low temperatures(T_(dust)<18 K)involved.Under such conditions,many molecules be... The initial condition of high-mass star formation is a complex area of study because of the high densities(n_(H_(2))>106cm^(-3))and low temperatures(T_(dust)<18 K)involved.Under such conditions,many molecules become depleted from the gas phase by freezing out onto dust grains.However,the N-bearing and deuterated species could remain gaseous under these extreme conditions,suggesting that they may serve as ideal tracers.In this paper,using the Plateau de Bure Interferometer and Very Large Array observations at 1.3 mm,3.5 mm,and 1.3 cm,we investigate the possible habitats for NH_(3),NH_(2)D,H^(13)CN,HC^(15)N,SO,and C^(18)O in eight massive precluster and protocluster clumps G18.17,G18.21,G23.97N,G23.98,G23.44,G23.97S,G25.38,and G25.71.We found that the NH3cores are in good agreement with the 3.5 mm peak emission,but the NH_(3)is much more extended than the 3.5 mm emission structure.The SO distributions agree well with the 3.5 mm peaks for the evolved star formation stage,but we did not detect any SO emission in the four earliest star formation sources.C^(18)O is a poor tracer in conditions of the cold(■18 K)and dense(■10^(4)cm^(-3))cores,e.g.,the prestellar cores.We also found that the NH_(2)D cores are mainly located in the temperature range of 13.0-20.0 K,and the NH_(2)D lines may be strongly depleted above 20 K. 展开更多
关键词 galaxies:star formation techniques:interferometric methods:observational
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