Endoplasmic reticulum stress improved chicken tenderness,promoted apoptosis and autophagy during postmortem ageing

In this study,endoplasmic reticulum(ER)stress inducer tunicamycin(TM)and inhibitor 4-phenylbutyric acid(4-PBA)were used to treat postmortem chicken breast muscle to investigate changes in tenderness and effects on apoptosis and autophagy during 5 days ageing.TM-induced ER stress reduced shear force,enhanced myofibril fragmentation index(MFI),disrupted myofibril structure,increased desmin degradation,and activatedμ-calpain and caspase-12.In addition,TM-induced ER stress increased the expression of Bax,Bim,and cytochrome c,and decreased the expression of Bcl-x L.Furthermore,TM-induced ER stress improved the conversion of LC3I to LC3II,raised the expression of Beclin-1,and decreased the expression of p62,PI3K,and m TOR.The opposite results were observed after 4-PBA treatment.These results suggested that ER stress could improve chicken tenderness,promote apoptosis and autophagy during chicken postmortem ageing.

High-quality Development of the Pearl River Night Tour in the Context of Deep Integration of Culture and Tourism

Urban night tourism is a new form of urban tourism development and an important driving force for the prosperity of urban night economy.As the most prestigious business card among many night tour projects in Guangzhou,the Pearl River water night tour has contributed to the development of Guangzhou s night economy,which cannot be underestimated.However,in the process of rapid development,there are also problems such as diversified operators,insufficient integration of intangible cultural elements,excessive commercial attributes,issues of tourism facilities and service facilities.Under the new stage of integrated development of culture and tourism,how to use mutual promotion of culture and tourism in the Pearl River night tour to seek innovative paths for high-quality development in the future is the main problem expected to be solved in this paper.Therefore,based on the perspective of culture and tourism integration,this paper analyzes the general situation and development status of the Pearl River night tour,and proposes five development strategies:the overall planning of culture and tourism integration,the deep integration of intangible cultural elements,the balance of commercial attributes and tourism attributes,the high integration of culture,business,tourism and education,and the creation of tourism atmosphere,in order to provide inspiration for the healthy,orderly and high-quality development of the Pearl River night tour.

A Way to Rootedness: Traveling in Tender Is the Night

Traveling is one major theme of Fitzgerald's novel Tender Is the Night. This fashionable activity for the 1920 s Americans is represented in the novel as ostensibly bearing different meanings to the main characters, but a deeper look would reveal that it is unanimously resorted to as a weapon against the spiritual barrenness caused by the cult of business, and more importantly, as a way to the experience of rootedness.

Short-term night lighting disrupts lipid and glucose metabolism in Zebra Finches:Implication for urban stopover birds

Night lighting has been shown to affect wild animals.To date,the effects of night lighting on the metabolic homeostasis of birds that spend short time in urban environments remain unclear.Using model bird species Zebra Finch(Taeniopygia guttata),we investigated the effects of short-term night lighting on liver transcriptome,blood glucose,triglyceride,and thyroxine(T4 and T3)levels in birds exposed to two different night lighting duration periods(three days and six days).After three days of night lighting exposure,the expression of genes involved in fat synthesis in the liver was upregulated while the expression of genes involved in fatty acid oxidation and triglyceride decomposition was downregulated.There was also a reduction in blood triglyceride,glucose,and T3 concentrations.However,after six days of night lighting,the expression of genes associated with fatty acid decomposition and hyperglycemia in the liver was upregulated,while the expression of genes involved in fat synthesis was downregulated.Simultaneously,blood glucose levels and T3 concentration increased.These findings indicate that short-term exposure to night lighting can disrupt the lipid and glucose metabolism of small passerine birds,and longer stopovers in urban area with intense night lighting may cause birds to consume more lipid energy.

High-quality Development Path of Night Tourism in Guangzhou:A Case Study of Firefly Night Tour

Night tourism often involves a large number of lighting facilities,which consume a large amount of energy.Therefore,one of the unique low energy consumption natural ecological tourism activities—firefly night tour has attracted attention and become an important breakthrough point for night tourism in tourist destinations.In this paper,Guangzhou firefly night tour project is taken as the research object.Based on the comprehensive economic,environmental,and socio-cultural benefits brought by the development of firefly night tour,the resources distribution,current development status,and existing problems of firefly night tour in Guangzhou are analyzed,and its high-quality development paths are proposed from three levels:government,industry,and tourist.The aim is to explore a new model for the economic development of Guangzhou night tour,boosting the transformation and upgrading of the night tourism economy,while also providing reference ideas and value for the development of night tourism economy in other tourist destinations.

Night Economy Glows with New Vitality

According to data from ii-Media Research,China’s night economy has grown rapidly since 2016.By the end of 2020,the scale of China’s night economy had exceeded RMB 30 trillion,an increase of 5.0%over the same period of last year,and it is expected to exceed RMB 48 trillion in 2023.According to the latest survey data of the Ocean Engine City Institute in 2023,more than 60%of respondents will go out for activities or consumption at night,among which citizens in high-speed cities have a higher preference for going out at night because of the relatively perfect urban infrastructure.

Single-image night haze removal based on color channel transfer and estimation of spatial variation in atmospheric light

The visible-light imaging system used in military equipment is often subjected to severe weather conditions, such as fog, haze, and smoke, under complex lighting conditions at night that significantly degrade the acquired images. Currently available image defogging methods are mostly suitable for environments with natural light in the daytime, but the clarity of images captured under complex lighting conditions and spatial changes in the presence of fog at night is not satisfactory. This study proposes an algorithm to remove night fog from single images based on an analysis of the statistical characteristics of images in scenes involving night fog. Color channel transfer is designed to compensate for the high attenuation channel of foggy images acquired at night. The distribution of transmittance is estimated by the deep convolutional network DehazeNet, and the spatial variation of atmospheric light is estimated in a point-by-point manner according to the maximum reflection prior to recover the clear image. The results of experiments show that the proposed method can compensate for the high attenuation channel of foggy images at night, remove the effect of glow from a multi-color and non-uniform ambient source of light, and improve the adaptability and visual effect of the removal of night fog from images compared with the conventional method.

Bonfire Night

In the UK,Bonfire Night or Guy Fawkes Night is celebrated on 5 November and the night skies are filled with colour.It’s a special day in honour of a historic event.

Night Vision Object Tracking System Using Correlation Aware LSTM-Based Modified Yolo Algorithm

Improved picture quality is critical to the effectiveness of object recog-nition and tracking.The consistency of those photos is impacted by night-video systems because the contrast between high-profile items and different atmospheric conditions,such as mist,fog,dust etc.The pictures then shift in intensity,colour,polarity and consistency.A general challenge for computer vision analyses lies in the horrid appearance of night images in arbitrary illumination and ambient envir-onments.In recent years,target recognition techniques focused on deep learning and machine learning have become standard algorithms for object detection with the exponential growth of computer performance capabilities.However,the iden-tification of objects in the night world also poses further problems because of the distorted backdrop and dim light.The Correlation aware LSTM based YOLO(You Look Only Once)classifier method for exact object recognition and deter-mining its properties under night vision was a major inspiration for this work.In order to create virtual target sets similar to daily environments,we employ night images as inputs;and to obtain high enhanced image using histogram based enhancement and iterative wienerfilter for removing the noise in the image.The process of the feature extraction and feature selection was done for electing the potential features using the Adaptive internal linear embedding(AILE)and uplift linear discriminant analysis(ULDA).The region of interest mask can be segmen-ted using the Recurrent-Phase Level set Segmentation.Finally,we use deep con-volution feature fusion and region of interest pooling to integrate the presently extremely sophisticated quicker Long short term memory based(LSTM)with YOLO method for object tracking system.A range of experimentalfindings demonstrate that our technique achieves high average accuracy with a precision of 99.7%for object detection of SSAN datasets that is considerably more than that of the other standard object detection mechanism.Our approach may therefore satisfy the true demands of night scene target detection applications.We very much believe that our method will help future research.

以提升患者就医体验为导向的中医药特色夜门诊实践探索

满足不同就医人群的需求,提供夜间诊疗服务,是改善患者就医体验的重要途径之一。夜门诊的开设有利于医院科学合理调整门诊空间,整合有限的医疗资源。为了提升患者就医体验,保障患者医疗安全,夜门诊开设需要优化医护技等人员的合理配置,加强医院多部门间的配合联动。浙江省中医院通过前期调研,结合中医医院特色、特长,开设具有中医药特色的夜门诊后,门诊运行效率进一步提高,医疗服务质量得到持续改进,得到患者的一致好评。

术前夜间血压与结肠癌患者腹腔镜微创全结肠系膜切除术后发生并发症的关系

目的探讨术前夜间血压和结肠癌患者腹腔镜微创全结肠系膜切除术后发生并发症的关系。方法选择2017年3月-2023年3月于该院行腹腔镜微创全结肠系膜切除术的174例结肠癌患者。将术前夜间血压下降率≥0%的患者作为下降组,例数为92例;将术前夜间血压下降率<0%的患者作为上升组,例数为82例。分别收集患者在入室时、手术开始即刻、手术开始至30 min后、手术开始至60 min后、手术开始至120 min后和手术完成时的平均动脉压(MAP)。记录患者住院期间术后并发症发生情况;采用多因素Logistic回归模型,分析结肠癌患者行腹腔镜微创全结肠系膜切除术后发生并发症的独立危险因素。结果术前,下降组糖尿病发生率与左心室质量指数明显低于上升组(P<0.05);术前24 h动态血压参数中,两组患者术前夜间舒张压与夜间收缩压比较,差异有统计学意义(P<0.05);两组患者术中MAP各时间点比较,差异均无统计学意义(P>0.05);两组患者手术开始后各时间点MAP明显低于入室时(P<0.05);下降组术后住院时急性肾损伤的发生率为10.87%,明显低于升高组的31.71%(P<0.05);多因素Logistic回归分析结果显示,术前夜间血压上升为结肠癌患者行腹腔镜微创全结肠系膜切除术后发生急性肾损伤的独立危险因素(P<0.05)。结论术前夜间血压上升为结肠癌患者行腹腔镜微创全结肠系膜切除术后发生急性肾损伤的独立危险因素。

不同贮藏温度下半胱氨酸氧化位点鉴定及对滩羊肉嫩度的影响

以宁夏滩羊肉为材料,借助半胱氨酸氧化修饰蛋白质组学技术,对-80℃(对照)和-2、-18、4℃条件下贮藏7 d滩羊肉的品质指标(剪切力和质构特性(硬度、弹性和咀嚼性))进行测定,同时对半胱氨酸氧化修饰位点进行鉴定,结合Pearson法对显著差异氧化位点(significantly differential oxidation sites,SDOS)的氧化强度与冷藏滩羊肉品质指标进行相关性分析。结果表明,与对照组相比,3个贮藏组的剪切力和质构特性均显著下降(P<0.05),25个SDOS对应的蛋白(10个结构蛋白、8个代谢酶、2个降解酶和5个其他蛋白)与至少1个品质指标显著相关(P<0.05),可作为羊肉贮藏期间嫩度和质构变化的潜在生物标志物。

电针辅助治疗类风湿关节炎的疗效观察及对血清类风湿因子和炎症因子的影响

目的观察电针足三里和关元穴辅助治疗类风湿关节炎(rheumatoid arthritis,RA)的临床疗效及对患者关节功能、关节压痛指数、红细胞沉降率、类风湿因子和炎症因子的影响。方法将112例RA患者用随机数字表法分为对照组和观察组,每组56例。对照组采用常规西医治疗,观察组在对照组治疗基础上予电针足三里和关元穴治疗。比较两组临床疗效和不良反应发生情况,观察两组治疗前后关节功能、关节肿胀指数、关节压痛指数、RA患者病情评价(disease activity score 28,DAS28)评分以及红细胞沉降率、类风湿因子、炎症因子[C-反应蛋白(C-reactive protein,CRP)、白介素-1β(interleukin-1β,IL-1β)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)]水平的变化。结果观察组总有效率高于对照组(P<0.05)。治疗后,两组关节功能(关节僵硬、疼痛和日常活动)评分、DAS28评分、关节肿胀指数和关节压痛指数以及红细胞沉降率、类风湿因子、CRP、TNF-α和IL-1β水平均低于治疗前(P<0.05),且观察组上述观察指标均低于对照组(P<0.05)。对照组1例出现咽部梗阻感,疑为甲氨蝶呤所致;有2例出现消化道不适症状。观察组2例出现针刺后皮下血肿,1例出现消化道不适症状。结论在常规西医治疗基础上,电针足三里和关元穴辅助治疗RA可提高临床疗效,有助于改善患者关节功能和关节压痛,降低红细胞沉降率、类风湿因子和炎症因子水平。

1990-2020年长三角地区生境质量与夜间灯光的空间关系

基于1990-2020年长三角地区的土地利用数据和夜间灯光数据,运用InVEST生境质量评估模型、双变量空间自相关、相关系数对生境质量与夜间灯光的时空关系进行研究,并应用地理加权回归和归纳法分析两者的响应规律,结果表明:(1)浙江省总体上生境最优且城镇发展最快,安徽省在城镇快速发展的同时生境相对下降最少,生境质量和夜间灯光空间分布格局呈现出与区位、地形等因素的空间耦合性。(2)长三角地区生境质量与夜间灯光指数随着时间的发展空间上的依赖性越来越强,聚类模式主要为高-低、低-高及低-低3种。高-低聚类区域主要分布在浙江省和安徽省的南部、西部的山地丘陵,研究期内呈现缩小的趋势;低-高聚类区域在长三角东部平原区呈集聚分布、北部平原丘陵区呈零散分布,呈现扩张趋势。(3)不同年份各省份生境质量与夜间灯光指数呈显著负相关,且经济越发达的省份,生境质量与夜间灯光指数的负相关关系越强;生境质量与夜间灯光指数呈高度负相关的区县主要分布在长江下游沿岸和浙江省东部,相关性不显著的区县主要分布在安徽省、江苏省的北部,变化类型以负相关关系持续增强型占比最高。(4)生境质量与夜间灯光指数的相关关系随着城市发展呈U型变化,即随着城市发展,生境质量与夜间灯光指数的负相关关系先增强后下降。研究结果可为长三角城市可持续发展、生境调控提供前期基础。

基于YOLOv5的茶叶嫩芽图像识别算法研究

现有的机器采茶都需要人工辅助进行采茶,且存在老叶、嫩芽一刀切的情况,会损害一部分茶叶,只适用于低端茶叶的采摘。因此,需要研究出一种精准高效的茶叶嫩芽识别方法。针对茶叶嫩芽图像背景复杂的问题,在YOLOv5算法的基础上,对算法进行多角度的改进,实验结果表明,改进算法的模型的mAP提升了4.1%,Recall提升了4.0%,且改进方法减少了漏检情况的发生。

主动退市背景下余股强制挤出制度的构建

余股强制挤出制度不是对余股股东股权的侵夺,而是将已缩小为财产性利益的股权转化为经济补偿请求权,故完全符合我国宪法的规定。余股强制挤出制度可更精准地平衡控股股东与余股股东的利益冲突且有效解决余股困境,应尽快引入我国法律。我国宜在要约收购型私有化退市中引入余股强制挤出制度,并遵循“或然关系模式”将挤出确定为私有化退市的特殊情形。应选择更为温和的“单一比例门槛”,并根据不同的股权分布情况设置差异化的挤出门槛阈值。在臂长交易情形,可直接将前序收购要约价格确定为挤出价格。在自我交易情形,宜制定整体主义视角下内外机制相结合的公平价格保障机制。鉴于挤出权与卖出权是互为镜像的一组权利,在引入挤出制度后,卖出权制度亟须进行联动修改。

关乎衰退的冒险之旅——格吕克诗集《忠贞之夜》中的衰老书写

“衰老”是路易丝·格吕克后期诗作的一大主题,在评论《忠贞之夜》时诗人称其承担了一次宏大的关乎“衰退”的冒险之旅。该文通过文本细读探究诗人如何展现“衰老”主题以及该主题如何折射诗人老年时期的内心世界。结合形式诗学和文化诗学分析,文章认为诗人通过聚焦“旅行”隐喻、重复使用相同意象并融入自身晚年经历,来展现“人”所面对的身体机能衰退、灵感枯竭等内在和外在困境。诗人对衰老或刻意或隐喻的书写,是对人生与艺术的不懈思考。

吕梁山两次夜间暴雨的边界层特征及能量来源与转换

利用常规观测、NCEP/NCAR 1°×1°再分析、FY-2E卫星数据等资料,对分别由β-中尺度持续拉长状对流系统(MβECS)和中尺度对流复合体(MCC)造成的两次吕梁山夜间暴雨过程进行数值模拟和粒子后向轨迹追踪,结果表明:过程1受边界层南风急流和西南气流影响,山西西部低空急流偏西分量和晋中盆地边界层西南气流的增强是对流不稳定能量重建的重要因子。过程2则受边界层南风和东风急流作用,南风急流被显著抬升到对流层中高层,形成含有冰晶层的中高层云系,东风急流则供应低空水汽。两次过程边界层(0.8-1.2 km)粒子携带的水汽均明显超过低层(1.5-3 km),是夜间短时强降水所需水汽的最大贡献者。700 hPa及以上非绝热作用产生扰动有效位能,之后向扰动动能的转化是两次过程中短时强降水发生所需能量的主要来源。

日本暗夜星空保护制度的建设及其对我国的启示

暗夜星空保护制度是一项保护美丽星空环境、防治光污染的新兴法律制度。从20世纪80年代以来,日本逐步确立了暗夜星空保护制度,并通过出台政策文件明确了星空保护区设定、照明器具限制使用、费用补偿、行政调查措施等核心规则,有力地提升了暗夜星空保护力度。就我国而言,可以借鉴域外法治经验,依循从地方到中央的立法思路,由星空资源富集区进行先行立法,基于分类管理理念确立暗夜星空保护区设定规则,基于规范与惩治相结合原则完善暗夜星空管理制度。

特克赛尔羊×阿勒泰羊F_(2)绵羊肉质嫩度全基因组关联分析

为了定位影响绵羊嫩度相关SNP位点及其重要候选基因,该研究选取462只特克赛尔羊×阿勒泰羊F_(2)绵羊,其中公羔229只、母羔233只,所有试验羊均在统一条件下饲养至8月龄后进行屠宰,利用C-LM4嫩度仪对嫩度进行测定,利用Illumina绵羊高密度(600K)SNP芯片进行基因分型,使用GEMMA软件的MLM模型对嫩度性状进行全基因组关联分析。结果表明,经质控后,剩余613178个SNPs位点用于全基因组关联分析,发现9个潜在SNPs位点与嫩度相关,分别分布在2、6、23、24号染色体上。根据基因生物学功能及相关研究文献,推测EREG、AREG、PDE1A基因参与肌肉再生、肌纤维形成等生物学过程,可作为影响嫩度的重要候选基因。研究结果可为利用分子生物学方法改良绵羊肌肉嫩度提供科学依据。