Effects of DNA extraction and universal primers on 16S rRNA gene-based DGGE analysis of a bacterial community from fish farming water

Among many reports investigating microbial diversity from environmental samples with denaturing gradient gel electrophoresis (DGGE), limited attention has been given to the effects of universal primers and DNA extraction on the outcome of DGGE analysis. In this study, these effects were tested with 16S rRNA gene-based DGGE on a bacterial community from farming water samples. The results indicate that the number of discernable bands in the DGGE fingerprint differed with the primer pairs used; the bands produced by 63f/518r, 341f/926r and 933f/1387r primer pairs were obviously fewer than those by 968f/1401r. Also, we found that each DNA extraction method resulted in different community profiles, reflected by the number and intensity of bands in the DGGE fingerprint. Furthermore, the main bands (theoretically representing dominant bacteria) differed with the extraction methods applied. It is therefore believed that the effects of universal primers and DNA extraction should be given more attention and carefully chosen before performing an investigation into a new environment with DGGE.

General Law of the Universe and Unity of All Universal Forces

All life forms in the universe have four-seasonal structure, four-seasonal movement, four gradations, and rise and fall of four gradations. This is the common feature of all life forms in the universe and the general law of the universe. The unity of the universe includes the unity of all universal forces which consist of two kinds, physical force and life force. The former includes bonding force, strong interaction, quark confinement, weak interaction, electromagnetic interaction and gravitational interaction and is also called four-seasonal structure. The latter lies inside the life forms, combines life beings and pushes the four-seasonal movement and rise-fall movement inside life forms. Life force includes transverse four-seasonal force, longitudinal four-seasonal force, four-gradation force, life being structural force and four-gradation rise-fail force. All these forces are united to form a complex life force system. Life force is also a compound, integral, long-range and automatically-closed four-seasonal force. Life being structural force is a force that connects all parts of a life being to make it an integral being. In the universe, life being structural force is an invisible three-dimensional net and a rigid structure and movement. It endows all heavenly bodies with a highly orderly structure and movement. Morality is the dominator of the universe. The whole universe is a moral system and all things in it have morality. Morality is also presented as a force, namely moral force, including rise force and fall force. The unity of all forces in the universe is the general law of the universe.

Floquet spectrum and universal dynamics of a periodically driven two-atom system

We investigate the Floquet spectrum and excitation properties of a two-ultracold-atom system with periodically driven interaction in a three-dimensional harmonic trap.The interaction between the atoms is changed by varying the s-wave scattering length in two ways,the cosine and the square-wave modulations.It is found that as the driving frequency increases,the Floquet spectrum exhibits two main features for both modulations,the accumulating and the spreading of the quasienergy levels,which further lead to different dynamical behaviors.The accumulation is associated with collective excitations and the persistent growth of the energy,while the spread indicates that the energy is bounded at all times.The initial scattering length,the driving frequency and amplitude can all significantly change the Floquet spectrum as well as the dynamics.However,the corresponding relation between them is valid universally.Finally,we propose a mechanism for selectively exciting the system to one specific state by using the avoided crossing of two quasienergy levels,which could guide preparation of a desired state in experiments.

Evaluating the Impact of the Universal Test and Treat Strategy on the Survival of Patients in the Northwest Region of Cameroon

Introduction: The main outcome of efficiently implemented universal test and treat (UTT) program is improved survival. UTT implementation has been ongoing in Cameroon since 2016 but evaluation data are scarce. This study aims to assess the survival of antiretroviral therapy (ART) patients initiated under UTT in Northwest region of Cameroon. Methods: This retrospective cohort study included HIV-positive patients initiated in 2016 at 27 purposefully selected sites and followed until 2021. Data was anonymously abstracted from ART registers and patients’ charts. Kaplan-Meier survival estimates and Cox model were used to compare the survival of patients initiated under UTT with those initiated otherwise, using stata version 14.0. Results: In total, 2490 HIV-positive patients (median age 42.7 years, 94.7% adults, and 69.0% female) participated in the study. Of 1389 patients with viral load (VL) test results, 55% were initiated on ART late. The VL suppression rate of patients initiated late and those initiated early were similar. During follow-up, 1020 (40.9%) participants censored. The survival curves of patients initiated early on ART and those initiated late were similar during the first 2.5 years of follow-up but significantly (p Conclusions: This study confirms the expected impact of UTT. Programs only need to close existing implementation gaps along the critical pathways (diagnosis and treatment) of UTT, focusing more on males.

Information Conductivity:Universal Performance Measure for Semantic Communications

As a novel paradigm,semantic communication provides an effective solution for breaking through the future development dilemma of classical communication systems.However,it remains an unsolved problem of how to measure the information transmission capability for a given semantic communication method and subsequently compare it with the classical communication method.In this paper,we first present a review of the semantic communication system,including its system model and the two typical coding and transmission methods for its implementations.To address the unsolved issue of the information transmission capability measure for semantic communication methods,we propose a new universal performance measure called Information Conductivity.We provide the definition and the physical significance to state its effectiveness in representing the information transmission capabilities of the semantic communication systems and present elaborations including its measure methods,degrees of freedom,and progressive analysis.Experimental results in image transmission scenarios validate its practical applicability.

Enhanced Temporal Correlation for Universal Lesion Detection

Universal lesion detection(ULD)methods for computed tomography(CT)images play a vital role in the modern clinical medicine and intelligent automation.It is well known that single 2D CT slices lack spatial-temporal characteristics and contextual information compared to 3D CT blocks.However,3D CT blocks necessitate significantly higher hardware resources during the learning phase.Therefore,efficiently exploiting temporal correlation and spatial-temporal features of 2D CT slices is crucial for ULD tasks.In this paper,we propose a ULD network with the enhanced temporal correlation for this purpose,named TCE-Net.The designed TCE module is applied to enrich the discriminate feature representation of multiple sequential CT slices.Besides,we employ multi-scale feature maps to facilitate the localization and detection of lesions in various sizes.Extensive experiments are conducted on the DeepLesion benchmark demonstrate that thismethod achieves 66.84%and 78.18%for FS@0.5 and FS@1.0,respectively,outperforming compared state-of-the-art methods.

Extensive numerical simulations on competitive growth between the Edwards–Wilkinson and Kardar–Parisi–Zhang universality classes

Extensive numerical simulations and scaling analysis are performed to investigate competitive growth between the linear and nonlinear stochastic dynamic growth systems, which belong to the Edwards–Wilkinson(EW) and Kardar–Parisi–Zhang(KPZ) universality classes, respectively. The linear growth systems include the EW equation and the model of random deposition with surface relaxation(RDSR), the nonlinear growth systems involve the KPZ equation and typical discrete models including ballistic deposition(BD), etching, and restricted solid on solid(RSOS). The scaling exponents are obtained in both the(1 + 1)-and(2 + 1)-dimensional competitive growth with the nonlinear growth probability p and the linear proportion 1-p. Our results show that, when p changes from 0 to 1, there exist non-trivial crossover effects from EW to KPZ universality classes based on different competitive growth rules. Furthermore, the growth rate and the porosity are also estimated within various linear and nonlinear growths of cooperation and competition.

Micro Renewal of Public Spaces in Communities Surrounding Colleges and Universities

The community located around colleges and universities is a place where university students and community residents engage in production and life together.Due to the influence of colleges and universities,these communities have spontaneously formed various other functions,resulting in the disorderly development of the entire space and a lack of effective guidance and control.With the development of colleges and universities,problems within the community are gradually increasing.These factors affect the spatial quality of the communities,as well as the environmental atmosphere of colleges and universities,and cause damage to the spatial environment of the entire region.By sorting out the issues between universities and surrounding communities,the development of universities is linked with that of communities,and a path of cooperation and win-win development is sought.

Sci-tech Simultaneous Interpreter Education Based on Translation Universals Research

The rapid evolution of scientific and technological advancements and industrial changes has profoundly interconnected countries and regions in the digital information era,creating a globalized environment where effective communication is paramount.Consequently,the demand for proficient interpreting skills within the scientific and technology sectors has surged,making effective language communication increasingly crucial.This paper explores the potential impact of translation universals on enhancing sci-tech simultaneous interpreter education.By examining the selection of teaching materials,methods,and activities through the lens of translation universals,this study aims to improve the quality of teaching content,innovate instructional approaches,and ultimately,enhance the effectiveness of interpreter education.The findings of this research are expected to provide valuable insights for curriculum development and pedagogical strategies in interpreter education.

From the Subjectivity of Human Rights to the Universality of Human Rights——A Preliminary Interpretation of the Theory on the Subjectivity of Human Rights

Universality is one of the essential characteristics of human rights, but there is a substantive difference between idealism and realism in understanding the concept of universality of human rights. The concepts of radical universality and strong universality based on the concept of natural human rights are too idealistic and thus have a high risk of being alienated into a tool of international re-pression in practice. On the contrary, the theory on the subjectivity of human rights can effectively defuse this risk brought about by radical universality and strong universality. Advocating a transition from the universality of human rights to the subjectivity of human rights not only keeps in line with the actual construction process of international human rights, but can also effectively interpret the practical difficul-ties in the realization process of international human rights. Therefore, it can be used as another alternative theory on human rights beyond the theory of natural human rights.

Introducing a 2nd Universal Space-Time Constant Can Explain the Observed Age of the Universe and Dark Energy

The purpose of this paper is to introduce new theoretical concepts as opposed to accepting the existence of dark entities, such as dark energy. This research sought to introduce a 2nd universal space-time constant, besides having a finite speed constant (speed of light in vacuum c). A finite universal age constant b is introduced. Namely, this paper shows that the changes in the Earth’s anomalistic year duration over time support the hypothesis of the age of the universe correlating with a maximum number of orbital revolutions constant. Neglecting the gravitational influence of other cosmological entities in the proximity of the Earth, the constant maximum number of revolutions is herewith determined solely by the Earth’s orbital revolutions around the Sun. The value of the universal age constant b is calculated to be around 13.8 billion orbital revolutions, derived out of an equation related to the changes in the Earth’s anomalistic year duration over time and the so-called Hubble tension. The above-mentioned calculated value b correlates well with the best fit to measured data of the cosmic microwave background radiation (CMBR) by the Planck spacecraft, the age of the observed universe is measured to be approximately 13.787 ± 0.020 billion years (2018 final data release). Developing a theory with this 2nd universal space-time constant b, being covariant with respect to the Lorentz transformations when time spans are large, gives results such as: A confirmation of the measured CMBR value of 13.787 ± 0.020 billion years. Correlating well with the observed expansion rate of the universe (dark energy). The universe’s expansion accelerating over the last four to five billion years.

Development and Optimization of Universal Bone Conduction Hearing Spectacles

Background: Non-implantable bone anchored hearing devices (BCHDs) are utilized for patients with conductive or mixed hearing loss who are unsuitable for conventional hearing aids or have unresolved middle ear issues. These devices can be surgically implanted or attached using adhesive plates, dental sticks, elastic headbands, or bone conduction spectacles. Optimal fitting of bone conduction spectacles requires appropriate frame selection and contact pressure in the temporal and mastoid areas. The ANSI S3.6 and DIN EN ISO 389-3 standards recommend a contact area of approximately 1.75 cm2 and a maximum force of 5.4 N for effective sound transmission and comfort. Methods: This study aimed to evaluate the technical fit and mechanical stability of universal bone conduction hearing spectacles compared to established systems. A Sen-Pressure 02 thin-film sensor connected to an Arduino Uno R3 board measured contact force in the temporal and mastoid areas. Several BCHDs were tested, including the Bruckhoff la belle BC D50/70, Radioear B71 headset, Radioear B71 elastic headband, Cochlear Baha SoundArc M, and Cochlear Baha elastic headband, on a PVC artificial head, with data analyzed using ANOVA and LSD post hoc tests. Results: The la belle BC D50/70 spectacles showed comparable contact force to established BCHDs, ensuring adequate sound transmission and comfort. Significant differences were observed between the systems, with the Radioear B71 headset exhibiting the highest forces. The la belle BC D50/70 had similar forces to the Radioear B71 elastic headband. Conclusion: The la belle BC D50/70 universal bone conduction hearing spectacles are a technically equivalent alternative to established BCHDs, maintaining pressure below 5.4 N. Future research should explore the impact of different contact forces on performance and comfort, and the integration of force control in modified spectacles. This study indicates that the la belle BC D50/70 is a viable alternative that meets audiological practice requirements.

An Analysis of the Path of Ethnic Unity and Progress Education under Academy-Based Education: Taking Ningxia University as an Example

Academy-based education is an active exploration and effective attempt to reform higher education and talent cultivation systems in the new era.This article aims to deeply analyze the characteristics and advantages of academy-based education.Combining the theoretical connotation and practical needs of ethnic unity and progress education and taking Ningxia University as an example,it explores the construction and implementation path of ethnic unity and progress education under academy-based education.It promotes communication and integration between students of different ethnic groups,enhances ethnic identity and national consciousness,and promotes the high-quality development of ethnic unity and progress cause.

Challenges and Opportunities for the Development of Ideological and Political Education in Colleges and Universities in the New Media Era

The rapid advancement of new media technology has had a great impact on people’s lifestyles,and brought opportunities and challenges to the development of ideological and political education in colleges and universities.Ideological and political education in colleges and universities is faced with the challenge of how to grasp the opportunity,ride on the express train of science and technology,and implement the concepts of“all-round education”and“five ideologies and politics.”

An In-depth Interpretation of the Universal Declaration of Human Rights With the Common Values of Humanity As the Research Paradigm

Interpreting the Universal Declaration of Human Rights from political,juridical and philosophical perspectives is es-sential for promoting the guiding principles of the Declaration,build-ing consensus on human rights,and advancing human rights practice in the new historical context.To conduct an academic,systematic in-terpretation of the Declaration that conforms to the trends of the times and answers the fundamental questions of the world,it is necessary to find a new research paradigm.The common values of humanity,namely peace,development,equity,justice,democracy and freedom,put forward by Xi Jinping,general secretary of the Communist Party of China(CPC)Central Committee,provide the most explanatory and penetrating scientific paradigm for reaching the issue.This paper an-alyzes and reflects on the views,value foundation and principled(con-tractual)consensus of human rights in the Declaration,and narrates and foresees the far-reaching significance of the three global initia-tives(namely,the Global Development Initiative,the Global Security Initiative,and the Global Civilization Initiative)with the common val-ues of humanity as the soul in advancing the modernization of global human rights governance and building a new form of human rights civilization.

Revisiting the Spirit of the UDHR and Discussing Human Rights Development——Summary of Views from the Seminar Commemorating the 75th Anniversary of the Universal Declaration of Human Rights

On December 4,2023,the China Society for Human Rights Studies hosted a seminar in Beijing commemorating the 75^(th) Anniversary of the Universal Declaration of Human Rights.Participants discussed topics such as the significance of the Universal Declaration of Human Rights,China’s theories and practices in respecting and safeguarding human rights,the three global initiatives and global human rights governance,human rights protection in the digital age,and telling Chinese stories of human rights in the new era.The discussions led to a broad consensus and achieved positive results.

Community-Based Health Insurance： An Evolutionary Approach to Achieving Universal Coverage in Low-Income Countries

The WHO World Health Assembly, and the most recent WHO World Health Report, have called for all health systems to move toward universal coverage. However, low-income countries have made little progress in this respect. We use existing evidence to describe the evolution of community-based health insurance in low-income countries through the three stages of basic model, enhanced model, and nationwide model. We have concluded that community-based health insurance development is a potential strategy to meet the urgent need for health financing in low-income countries. With careful planning and implementation, it is possible to adopt such evolutionary approach to achieve universal coverage by extending tax-based financing/social insurance characteristics to community-based health insurance schemes.

Challenges faced by community health nurses to achieve universal health coverage in Myanmar:A mixed methods study

Objective:This study aimed to identify the challenges of community health nurses(CHNs)in delivering effective community health care to achieve universal health coverage(UHC)in Myanmar.Methods:A total of 30 CHNs from township health centers in the northeastern,southern,and western parts of Myanmar were purposefully recruited for quantitative and qualitative interviews.Quantitative data were processed using Microsoft Excel software,and qualitative data were analyzed using thematic analysis.This study is registered with researchregistry6201.Results:Around the country,30 CHNs uncovered their hardships in implementing primary health care to achieve UHC.Over 90%of the participants agreed to the problem of inadequate health infrastructure,while half of them felt unmotivated when they encountered role conflicts among various cadres of healthcare providers and poor opportunities for career promotion.Major problems arose from the lack of standard professional education at the entry point to community settings because most CHNs did not achieve specialized training in providing public health services.Complications are incapable of evaluating health services for policy-making and the inability to conduct health research to develop evidencebased practices.Insecure work and living conditions,unsupportive community relationships,and undereducation in professional practices were supportive major themes explored by CHNs to achieve a deeper understanding of the barriers to UHC.Not only the health system itself but also the population and other geographical factors have contributed to many challenges to CHNs.Conclusion:Myanmar’s CHNs face many challenges in achieving UHC.These challenges are not confined to the health sector.Some situations,such as geographical barriers and transportation problems,remain persistent challenges for healthcare providers.This study highlights the fact that current health systems should be strengthened by qualified healthcare providers and sufficient infrastructure.Meanwhile,public empowerment plays a critical role in promoting health development.

From the UN Charter and the Universal Declaration of Human Rights to Building a Community with a Shared Future for Human Beings--The Past, Present, and Future of International Human Rights Protection

The UN Charter first of all affirmed the basic concept of international protection of human rights and opened up a broad space for international protection of human rights. The Universal Declaration of Human Rights is an important document in the development of the international human rights cause. It established a set of international standards for basic human rights, represents the world’s most important consensus on human rights, and contributes greatly to the creation of an international human rights legal system. The important theory put forward by President Xi Jinping to build a Community with a Shared Future for Human Beings, as a Chinese program for today s global major issues, also outlines a new blueprint for national human rights protection and will play a major role in promoting the development of China’s human rights and international human rights protection.

ProTaper Universal再治疗系统去除根管内充填物的体外研究

分别采取2种方法去除离体上前牙根管内充填物。A组:ProTaper Universal再治疗锉(D1、D2、D3);B组:K锉+丁克除(Desocclusol)。记录操作时间和根管内充填物残留情况。A组操作时间明显短于B组(P<0.05);A组根管清洁程度高于B组,但差异无显著性(P>0.05)。ProTaper Universal再治疗锉去除根管内充填物安全、快速、有效。