Food safety is a major issue to public health and have attracted global attention.Fast,sensitive,and reliable detection methods for food hazardous substances is highly desirable.Aptamers which can bind to the target m...Food safety is a major issue to public health and have attracted global attention.Fast,sensitive,and reliable detection methods for food hazardous substances is highly desirable.Aptamers which can bind to the target molecules with high affinity and specificity represent an attractive tool for the recognition of food hazardous substances,which play an important role in the development and application of new food safety detection technology.But current assays for characterizing small molecule-aptamer binding are limited by either the mass sensitivity or the size differentiation ability.Herein,we proposed a comprehensive method for assessing the dissociation equilibria of small molecule-aptamer,which is immobilized-free under ambient conditions.The design employs the Le Chatelier’s principle and could be used to effectively measure small molecule-aptamer interactions.ATP binding aptamer and anti-aflatoxin B1 aptamer were used as the model system to determine their affinity,in which their dissociation equilibria measurements are in excellent close to their previous work.Due to the simplicity and sensitivity of this new method,we believe that it could be recommended as an effective tool for characterizing small molecule-aptamer interactions and promote the further application of small molecular aptamer in food safety.展开更多
The effects of alloying elements and processing parameters on the mechanical properties and Portevin-Le Chatelier effect of A1-Mg alloys developed for inner auto body sheets were investigated in detail. Tensile testin...The effects of alloying elements and processing parameters on the mechanical properties and Portevin-Le Chatelier effect of A1-Mg alloys developed for inner auto body sheets were investigated in detail. Tensile testing was performed in various Zn and Mg contents under different annealing and cold-rolling conditions. In the results, the stress drop and reloading time of serrations increase with increasing plastic strain and exhibit a common linear relationship. The increase rates of stress drop and reloading time increase with increasing Mg or Zn content. The alloys with a greater intensity of serrated yielding generally exhibit a greater elongation. The stress drop and reloading time of serrations decrease with increasing grain size in the case of the annealed samples. The cold-rolled sample exhibits the most severe serra- tion because it initially contains a large number of grain boundaries and dislocations.展开更多
In this paper, solute concentration and precipitate content in A2024 aluminum alloy are adjusted by solution treatment （ST） at different temperatures and tensile experiments on these treated specimens are carried ou...In this paper, solute concentration and precipitate content in A2024 aluminum alloy are adjusted by solution treatment （ST） at different temperatures and tensile experiments on these treated specimens are carried out. It is found that the temperature of solution treatment （ST temperature） has a remarkable influence on the amplitude of the serrated flow and the propagation characteristics of shear bands. These results are due to the effects of solute atoms and precipitates on dynamic strain aging （DSA）. When ST temperature is higher than 300 ℃, solute concentration is relatively high and solute cloud is a key factor of DSA. When ST temperature is lower than 300 ℃, precipitate content is relatively high and the mechanism of DSA is determined by precipitates.展开更多
In this paper a technique based on high-speed digital photography and the digital speckle correlation （DSC） method is used for the quantitative measurement of the displacement and strain fields of various Portevin L...In this paper a technique based on high-speed digital photography and the digital speckle correlation （DSC） method is used for the quantitative measurement of the displacement and strain fields of various Portevin Le Chatelier （PLC） bands （types A, B, and C）. The experimental results clearly show the nucleation process of a type-B band and the propagation of a type-A band. The results also reveal that there exists an elastic shrinkage deformation outside a PLC band during a large avalanche-like deformation inside the PLC band.展开更多
In the alloy with solute content higher than the limiting solubility,the solute atoms that have failed to dissolve will precipitate from the solid solution and form precipitations.In this study, the Portevin-Le Chatel...In the alloy with solute content higher than the limiting solubility,the solute atoms that have failed to dissolve will precipitate from the solid solution and form precipitations.In this study, the Portevin-Le Chatelier(PLC) effects in annealed 5456 and 5052 aluminum alloys with different precipitation contents have been investigated under different applied strain rates.The results suggest that precipitations have significant effect on the PLC effect and the more the precipitations are, the greater the influence is.Furthermore,the solute diffusion is pipe diffusion in 5052 alloy with lower precipitation content.However,for 5456 alloy with higher precipitation content,the diffusion is no longer the case but more complex.展开更多
Portevin-Le Chatelier (PLC) effect in a severely cold-rolled Al-Zn-Mg-Cu-Zr aluminum alloy (AA7055 type) plate before and after annealing was investigated. The results show that the sample annealed at 300 ℃ undergoes...Portevin-Le Chatelier (PLC) effect in a severely cold-rolled Al-Zn-Mg-Cu-Zr aluminum alloy (AA7055 type) plate before and after annealing was investigated. The results show that the sample annealed at 300 ℃ undergoes PLC effect,whereas the PLC effect is not observed in the deformed sample or the samples annealed at 110 ℃ or 200 ℃. The main precipitates in the sample annealed at 300 ℃ are the coarsened η phases,while a mixture of small η' phases and η phases dominates in the sample annealed at 200 ℃. In the deformed sample and sample annealed at 110 ℃,GP zone are the main precipitates. The different flow behaviors are ascribed to the type of precipitates as well as the dislocation density differences.展开更多
基金This work was supported by the National Natural Seience Foundation of China under gran No.10372098 and 10232030.We would also like to thank the Lab of Mechanical&Materials Science of USTC for their help in the experiments on MTS
基金supported by the National Key R&D Program of China(2017YFC1600603)the Funds for Huangshan Professorship of Hefei University of Technology(407-037019).
文摘Food safety is a major issue to public health and have attracted global attention.Fast,sensitive,and reliable detection methods for food hazardous substances is highly desirable.Aptamers which can bind to the target molecules with high affinity and specificity represent an attractive tool for the recognition of food hazardous substances,which play an important role in the development and application of new food safety detection technology.But current assays for characterizing small molecule-aptamer binding are limited by either the mass sensitivity or the size differentiation ability.Herein,we proposed a comprehensive method for assessing the dissociation equilibria of small molecule-aptamer,which is immobilized-free under ambient conditions.The design employs the Le Chatelier’s principle and could be used to effectively measure small molecule-aptamer interactions.ATP binding aptamer and anti-aflatoxin B1 aptamer were used as the model system to determine their affinity,in which their dissociation equilibria measurements are in excellent close to their previous work.Due to the simplicity and sensitivity of this new method,we believe that it could be recommended as an effective tool for characterizing small molecule-aptamer interactions and promote the further application of small molecular aptamer in food safety.
基金financially supported by the National Natural Science Foundation of China(No.51301017)the Fundamental Research Funds for the Central Universities of China(No.FRF-TP-13-034A)
文摘The effects of alloying elements and processing parameters on the mechanical properties and Portevin-Le Chatelier effect of A1-Mg alloys developed for inner auto body sheets were investigated in detail. Tensile testing was performed in various Zn and Mg contents under different annealing and cold-rolling conditions. In the results, the stress drop and reloading time of serrations increase with increasing plastic strain and exhibit a common linear relationship. The increase rates of stress drop and reloading time increase with increasing Mg or Zn content. The alloys with a greater intensity of serrated yielding generally exhibit a greater elongation. The stress drop and reloading time of serrations decrease with increasing grain size in the case of the annealed samples. The cold-rolled sample exhibits the most severe serra- tion because it initially contains a large number of grain boundaries and dislocations.
基金supported by the National Natural Science Foundation of China (Grant Nos 10872189 and 10732080)
文摘In this paper, solute concentration and precipitate content in A2024 aluminum alloy are adjusted by solution treatment （ST） at different temperatures and tensile experiments on these treated specimens are carried out. It is found that the temperature of solution treatment （ST temperature） has a remarkable influence on the amplitude of the serrated flow and the propagation characteristics of shear bands. These results are due to the effects of solute atoms and precipitates on dynamic strain aging （DSA）. When ST temperature is higher than 300 ℃, solute concentration is relatively high and solute cloud is a key factor of DSA. When ST temperature is lower than 300 ℃, precipitate content is relatively high and the mechanism of DSA is determined by precipitates.
基金Project supported by the National Natural Science Foundation of China （Grant Nos 1023030 and 10372098）.
文摘In this paper a technique based on high-speed digital photography and the digital speckle correlation （DSC） method is used for the quantitative measurement of the displacement and strain fields of various Portevin Le Chatelier （PLC） bands （types A, B, and C）. The experimental results clearly show the nucleation process of a type-B band and the propagation of a type-A band. The results also reveal that there exists an elastic shrinkage deformation outside a PLC band during a large avalanche-like deformation inside the PLC band.
基金The financial support received from the National Natural Science Foundation of China under Grant Nos. 10732080,10872189
文摘In the alloy with solute content higher than the limiting solubility,the solute atoms that have failed to dissolve will precipitate from the solid solution and form precipitations.In this study, the Portevin-Le Chatelier(PLC) effects in annealed 5456 and 5052 aluminum alloys with different precipitation contents have been investigated under different applied strain rates.The results suggest that precipitations have significant effect on the PLC effect and the more the precipitations are, the greater the influence is.Furthermore,the solute diffusion is pipe diffusion in 5052 alloy with lower precipitation content.However,for 5456 alloy with higher precipitation content,the diffusion is no longer the case but more complex.
基金Project(2005CB23705) supported by the National Basic Research Program of China
文摘Portevin-Le Chatelier (PLC) effect in a severely cold-rolled Al-Zn-Mg-Cu-Zr aluminum alloy (AA7055 type) plate before and after annealing was investigated. The results show that the sample annealed at 300 ℃ undergoes PLC effect,whereas the PLC effect is not observed in the deformed sample or the samples annealed at 110 ℃ or 200 ℃. The main precipitates in the sample annealed at 300 ℃ are the coarsened η phases,while a mixture of small η' phases and η phases dominates in the sample annealed at 200 ℃. In the deformed sample and sample annealed at 110 ℃,GP zone are the main precipitates. The different flow behaviors are ascribed to the type of precipitates as well as the dislocation density differences.
基金Projects(52001249,51761135031,51790482,51722104)supported by the National Natural Science Foundation of ChinaProject(2017YFB0702301)supported by the National Key Research and Development Program of ChinaProject(2019M653595)supported by the China Postdoctoral Science Foundation。
