Objective To investigate integration and expression of adeno-associated virus (AAV) vectors in neuronal PC12 cells,the result of which can be applied in further gene therapy of diseases of the central nervous sys- tem...Objective To investigate integration and expression of adeno-associated virus (AAV) vectors in neuronal PC12 cells,the result of which can be applied in further gene therapy of diseases of the central nervous sys- tem. Methods Human neurotrophin-3(hNT3)genes were inserted into AAV vectors. Then the recombinat AAV plas- mids were encapsidated as recombinant virions. PCl2 cells were transfected with the virions and the positive cells were selected by G418. The transfection positive (hNT3 modified)PC12 cells were cultured for several generations and the cellular genomic DNA and total RNA were extracted. We investigated the integration locus or AAV vectors by South- ern blot and transcript situation or foreign genes by dot blot. Results The hybridization tests showed that AAV in- troduced foreign genes were stably integrated, but at random locus, and robustly transcribed in hNT3 modified PCl2 cells. Conclusion AAV vectors can serve as high efficiency vectors or target genes in neuronal PC12 cells.展开更多
For the hypersurface Γ=(y,γ(y)), the singular integral operator along Γ is defined by. Kf(x,x_n)=P.V.∫_R^(nl), f(x-y,x_n-γ(y))_(|y|^(n-1))^(Ω(y))dy, where Ω is homogeneous of order 0, ∫_∑_(1)Ω(y')dy'...For the hypersurface Γ=(y,γ(y)), the singular integral operator along Γ is defined by. Kf(x,x_n)=P.V.∫_R^(nl), f(x-y,x_n-γ(y))_(|y|^(n-1))^(Ω(y))dy, where Ω is homogeneous of order 0, ∫_∑_(1)Ω(y')dy'=0, For a certain class of hypersurfaces. T is shown to be bounded on L^p(R^n) provided Ω∈L_α~l(Σ_(n-2)),P>1.展开更多
Hosted by ChinaAssociation of Chinese Medicine,and organized by World Journal of Integrated traditional and western Medicine,seminar on clinical application of new technology of integrated Chinese and western medicine...Hosted by ChinaAssociation of Chinese Medicine,and organized by World Journal of Integrated traditional and western Medicine,seminar on clinical application of new technology of integrated Chinese and western medicine in treating sequelae of cerebrovascular diseases is planned to be held in Nanning,Guangxi in August.展开更多
面向“30·60”双碳目标,矿区能源利用方式的绿色、经济、高效转型成为我国能源革命的迫切需求。西部矿区拥有丰富的可再生能源资源禀赋,但仍面临着可再生能源就地消纳困难,电力设备投资成本高、利用率低以及外送输电通道有限的困...面向“30·60”双碳目标,矿区能源利用方式的绿色、经济、高效转型成为我国能源革命的迫切需求。西部矿区拥有丰富的可再生能源资源禀赋,但仍面临着可再生能源就地消纳困难,电力设备投资成本高、利用率低以及外送输电通道有限的困难。为提升矿区用能清洁化程度,提升矿区能源供给的稳定性与可靠性,增强矿区对外部电网的支撑能力,提出全清洁能源下的高品质矿区能源系统(High-quality Coal Mine Energy System,HCMES)及其配置优化方法。首先,考虑西部矿山综合能源系统的负荷特点与伴生能源利用,结合可再生能源发电与废弃矿井抽水蓄能,构建全清洁能源下的HCMES架构。其次,考虑到矿区生产全流程负荷的需求响应能力,考虑系统的能量平衡约束,提出全清洁能源下的高品质矿区能源系统优化配置模型。最后,以系统年平均综合成本最小化为目标,将原问题转化为混合整数线性规划模型,求解生成高品质矿区能源系统优化配置方案。以我国西部某年产煤量1200万t的矿区实际数据为实例,验证所提模型与方法的有效性,并分析可再生能源出力与生产负荷需求不确定性对系统优化配置结果的影响。算例仿真设置了4种矿区能源系统配置方式:不配置储能、配置抽水蓄能、配置电化学储能、配置抽水蓄能(不外购电能)。结果表明,所提出的HCMES相较于其他配置方式可减少电气一次设备投资11.11%,相较于方式3可降低年平均综合成本7.91%,且最多可减少矿区生产用能总二氧化碳排放量91.17%。展开更多
文摘Objective To investigate integration and expression of adeno-associated virus (AAV) vectors in neuronal PC12 cells,the result of which can be applied in further gene therapy of diseases of the central nervous sys- tem. Methods Human neurotrophin-3(hNT3)genes were inserted into AAV vectors. Then the recombinat AAV plas- mids were encapsidated as recombinant virions. PCl2 cells were transfected with the virions and the positive cells were selected by G418. The transfection positive (hNT3 modified)PC12 cells were cultured for several generations and the cellular genomic DNA and total RNA were extracted. We investigated the integration locus or AAV vectors by South- ern blot and transcript situation or foreign genes by dot blot. Results The hybridization tests showed that AAV in- troduced foreign genes were stably integrated, but at random locus, and robustly transcribed in hNT3 modified PCl2 cells. Conclusion AAV vectors can serve as high efficiency vectors or target genes in neuronal PC12 cells.
文摘For the hypersurface Γ=(y,γ(y)), the singular integral operator along Γ is defined by. Kf(x,x_n)=P.V.∫_R^(nl), f(x-y,x_n-γ(y))_(|y|^(n-1))^(Ω(y))dy, where Ω is homogeneous of order 0, ∫_∑_(1)Ω(y')dy'=0, For a certain class of hypersurfaces. T is shown to be bounded on L^p(R^n) provided Ω∈L_α~l(Σ_(n-2)),P>1.
文摘Hosted by ChinaAssociation of Chinese Medicine,and organized by World Journal of Integrated traditional and western Medicine,seminar on clinical application of new technology of integrated Chinese and western medicine in treating sequelae of cerebrovascular diseases is planned to be held in Nanning,Guangxi in August.
文摘面向“30·60”双碳目标,矿区能源利用方式的绿色、经济、高效转型成为我国能源革命的迫切需求。西部矿区拥有丰富的可再生能源资源禀赋,但仍面临着可再生能源就地消纳困难,电力设备投资成本高、利用率低以及外送输电通道有限的困难。为提升矿区用能清洁化程度,提升矿区能源供给的稳定性与可靠性,增强矿区对外部电网的支撑能力,提出全清洁能源下的高品质矿区能源系统(High-quality Coal Mine Energy System,HCMES)及其配置优化方法。首先,考虑西部矿山综合能源系统的负荷特点与伴生能源利用,结合可再生能源发电与废弃矿井抽水蓄能,构建全清洁能源下的HCMES架构。其次,考虑到矿区生产全流程负荷的需求响应能力,考虑系统的能量平衡约束,提出全清洁能源下的高品质矿区能源系统优化配置模型。最后,以系统年平均综合成本最小化为目标,将原问题转化为混合整数线性规划模型,求解生成高品质矿区能源系统优化配置方案。以我国西部某年产煤量1200万t的矿区实际数据为实例,验证所提模型与方法的有效性,并分析可再生能源出力与生产负荷需求不确定性对系统优化配置结果的影响。算例仿真设置了4种矿区能源系统配置方式:不配置储能、配置抽水蓄能、配置电化学储能、配置抽水蓄能(不外购电能)。结果表明,所提出的HCMES相较于其他配置方式可减少电气一次设备投资11.11%,相较于方式3可降低年平均综合成本7.91%,且最多可减少矿区生产用能总二氧化碳排放量91.17%。
