Duo to fluctuations in atmospheric turbulence and yaw control strategies,wind turbines are often in a yaw state.To predict the far wake velocity field of wind turbines quickly and accurately,a wake velocity model was ...Duo to fluctuations in atmospheric turbulence and yaw control strategies,wind turbines are often in a yaw state.To predict the far wake velocity field of wind turbines quickly and accurately,a wake velocity model was derived based on the method of momentum conservation considering the wake steering of the wind turbine under yaw conditions.To consider the shear effect of the vertical incoming wind direction,a two-dimensional Gaussian distribution function was introduced to model the velocity loss at different axial positions in the far wake region based on the assumption of nonlinear wake expansion.This work also developed a“prediction-correction”method to solve the wake velocity field,and the accuracy of the model results was verified in wake experiments on the Garrad Hassan wind turbine.Moreover,a 33-kW two-blade horizontal axis wind turbine was simulated using this method,and the results were compared with the classical wake model under the same parameters and the computational fluid dynamics(CFD)simulation results.The results show that the nonlinear wake model well reflected the influence of incoming flow shear and yaw wake steering in the wake velocity field.Finally,computation of the wake flow for the Horns Rev offshore wind farm with 80 wind turbines showed an error within 8%compared to the experimental values.The established wake model is less computationally intensive than other methods,has a faster calculation speed,and can be used for engineering calculations of the wake velocity in the far wakefield of wind turbines.展开更多
Large atmospheric boundary layer fluctuations and smaller turbine-scale vorticity dynamics are separately hypothesized to initiate the wind turbine wake meandering phenomenon,a coherent,dynamic,turbine-scale oscillati...Large atmospheric boundary layer fluctuations and smaller turbine-scale vorticity dynamics are separately hypothesized to initiate the wind turbine wake meandering phenomenon,a coherent,dynamic,turbine-scale oscillation of the far wake.Triadic interactions,the mechanism of energy transfers between scales,manifest as triples of wavenumbers or frequencies and can be characterized through bispectral analyses.The bispectrum,which correlates the two frequencies to their sum,is calculated by two recently developed multi-dimensional modal decomposition methods:scale-specific energy transfer method and bispectral mode decomposition.Large-eddy simulation of a utility-scale wind turbine in an atmospheric boundary layer with a broad range of large length-scales is used to acquire instantaneous velocity snapshots.The bispectrum from both methods identifies prominent upwind and wake meandering interactions that create a broad range of energy scales including the wake meandering scale.The coherent kinetic energy associated with the interactions shows strong correlation between upwind scales and wake meandering.展开更多
OBJECTIVE The inhibitory effect of active ingredients of Tripterygium wilfordii Hook.F.(TWHF)(celastrol,triptolide,triptonide,wilforlide A,wilforgine and wilforine)on human carboxylester⁃ase 1(CES1)and CES2 was detect...OBJECTIVE The inhibitory effect of active ingredients of Tripterygium wilfordii Hook.F.(TWHF)(celastrol,triptolide,triptonide,wilforlide A,wilforgine and wilforine)on human carboxylester⁃ase 1(CES1)and CES2 was detected to investigate the herb-drug interactions(HDIs)of TWHF.METHODS Human liver microsomes catalysed hydrolysis of 2-(2-benzoyl-3-methoxyphenyl)benzothi⁃azole(BMBT)and fluorescein diacetate(FD)were used as the probe reaction to phenotype the activity of CES1 and CES2,respectively.The residual activities of CES1 and CES2 were detected by ultrahigh performance liquid chromatography(UPLC)after intervention with celastrol,triptolide,triptonide,wilforlide A,wilforgine and wilforine(100μmol·L^(-1)).Kinetics analysis,involving half inhibitory concentra⁃tion(IC_(50)),inhibition type and kinetic parameter(Ki),and in vitro-in vivo extrapolation(IVIVE),was carried out to predict the HDIs between these compounds and CES-metabolizing drugs.Molecular docking was performed to analyze the ligand-enzyme interaction.RESULTS Out of the six main con⁃stituents of TWHF,only celastrol exhibited strong inhibition towards both CES1 and CES2,with the inhibitory rates of 97.45%(P<0.05)and 95.62%(P<0.05),respectively.The IC_(50)was 9.95 and 4.02 mol·L^(-1),respectively,and the types of inhibition were all non-competitive inhibition.Based on the kinetics analysis,the Ki values were calculated to be 5.10 and 10.55μmol·L^(-1)for the inhibition of celastrol on CES1 and CES2,respectively.IVIVE indicated that celastrol might disturb the metabolic hydrolysis of clinical drugs in vivo by inhibiting CES1.Molecular docking results showed that hydrogen bonds and hydrophobic contacts contributed to the interaction of celastrol and CESs.CONCLUSION The inhibitory effect of celastrol on CES1 and CES2 might cause HDIs with clinical drugs hydrolysed by CESs.展开更多
Objective:To investigate the hook effect in the detection of beta2-microglobulin(β2-MG)with different reagents as well as on two fully automated biochemical analyzers and their solutions.Methods:Using immunoturbidime...Objective:To investigate the hook effect in the detection of beta2-microglobulin(β2-MG)with different reagents as well as on two fully automated biochemical analyzers and their solutions.Methods:Using immunoturbidimetric assay forβ2-MG as the research object,β2-MG levels were detected by different concentration gradients ofβ2-microglobulin samples in Liedemann,Mack,and Myriad reagents of three manufacturers on two automatic biochemical analyzers,and the difference of the hook effect was comparatively analyzed.Results:Leadman reagent showed the hook effect on the Beckman AU5800 automated biochemistry analyzer,while both Maccura and Mindray reagents did not show the hook effect.After the experiments,we found the limit value of the pre-zone check of Leadman reagent,and changed the parameters of the instrument,when the limit value of the pre-zone check was reached,the instrument automatically diluted the specimen five times and then detected it again.After changing the parameters of the instrument,the correlation between the three methods of detecting samples of different concentrations was r>0.99.Conclusion:Before selecting the application of immunoturbidimetric reagents,we have to carry out the risk assessment of the hook effect and selectively set the parameters of the pre-zone check based on the highest concentration that may occur in the clinic;for the items that may have the hook effect,we have to selectively set the parameters of the pre-zone check when the ratio of average reaction rate and the immediate reaction rate is at the limit value,and the phenomenon of antigen excess may occur,the instrument will carry out automatic dilution before detection,so as to avoid the issuance of erroneous results of high-value samples due to the hook effect.展开更多
The effective utilisation of steel fibre reinforced concrete (SFRC) requires in-depth and detailed understanding of bonding mechanisms governing the tensile behaviour. In response to this demand, this study presents a...The effective utilisation of steel fibre reinforced concrete (SFRC) requires in-depth and detailed understanding of bonding mechanisms governing the tensile behaviour. In response to this demand, this study presents an empirical description of the axial force required to pull out a hooked end steel fibre from a cementitious matrix. It is based upon consistent experimental tests that show the force versus displacement plot is formed from a sequence of events in which partial and full debonding at the interface is followed by bending of the hook knee to raise the force to its maximum. A loss of peak force occurs with the reversed plasticity involved in a full straightening of the fibre that precedes the rapid sliding to its full removal under a falling force. The stages are assembled in the said order with a piecewise connection between linear segments and a curve of exponential decay. A power law can be introduced to describe the knee bending stage if preferred. The normalised co-ordinates were adopted for the simple mathematical discontinuous function of the full pull-out process. Normalising force and displacement for hooked fibre is based upon the maximum values found for straight fibre pull out. This would apply to an overall embedded length of unity and a unit peak force for a full debonding of straight fibre in similar materials. For hooked fibre the normalised co-ordinates given refer to the initiation and duration of each event to be tabulated at fractions of the embedded length with multiples of that force. Such predictions are seen to be in good agreement with average pull-out response curves.展开更多
Wind farms generally consist of a single turbine installed with the same hub height. As the scale of turbines increases,wake interference between turbines becomes increasingly significant, especially for floating wind...Wind farms generally consist of a single turbine installed with the same hub height. As the scale of turbines increases,wake interference between turbines becomes increasingly significant, especially for floating wind turbines(FWT).Some researchers find that wind farms with multiple hub heights could increase the annual energy production(AEP),while previous studies also indicate that wake meandering could increase fatigue loading. This study investigates the wake interaction within a hybrid floating wind farm with multiple hub heights. In this study, FAST.Farm is employed to simulate a hybrid wind farm which consists of four semi-submersible FWTs(5MW and 15MW) with two different hub heights. Three typical wind speeds(below-rated, rated, and over-rated) are considered in this paper to investigate the wake meandering effects on the dynamics of two FWTs. Damage equivalent loads(DEL) of the turbine critical components are computed and analyzed for several arrangements determined by the different spacing of the four turbines. The result shows that the dynamic wake meandering significantly affects downstream turbines’ global loadings and load effects. Differences in DEL show that blade-root flapwise bending moments and mooring fairlead tensions are sensitive to the spacing of the turbines.展开更多
SIX thousand kilometers!10,000 li! 3000 yearsold! The only man-madestructure that can be seenfrom the moon! The bricks alonewould build four Egyptian pyra-mids! Wide enough for fourhorses abreast! You are not aman unt...SIX thousand kilometers!10,000 li! 3000 yearsold! The only man-madestructure that can be seenfrom the moon! The bricks alonewould build four Egyptian pyra-mids! Wide enough for fourhorses abreast! You are not aman until you’ve been to theWall! We have all heard at leastone of these claims.展开更多
China ramps up its efforts to safeguard juveniles from the Internet gaming addiction Just before this year’s national college entrance exam,or the gaokao,on June 7-8,a letter from a despairing mother of student made ...China ramps up its efforts to safeguard juveniles from the Internet gaming addiction Just before this year’s national college entrance exam,or the gaokao,on June 7-8,a letter from a despairing mother of student made a big splash on Weibo,a Chinese Twitter-like microblogging platform.The mother described how her son,who used to be an outstanding learner,failed the exam展开更多
基金Supported by the Key R&D Program of Shandong Province,China(No.2023ZLYS01)the National Key R&D Program of China(No.2022YFC3104200)+2 种基金the National Natural Science Foundation of China(No.12302301)the China Postdoctoral Science Foundation(No.2023M742229)the Zhejiang Provincial Natural Science Foundation(ZJNSF)(No.LQ22F030002)。
文摘Duo to fluctuations in atmospheric turbulence and yaw control strategies,wind turbines are often in a yaw state.To predict the far wake velocity field of wind turbines quickly and accurately,a wake velocity model was derived based on the method of momentum conservation considering the wake steering of the wind turbine under yaw conditions.To consider the shear effect of the vertical incoming wind direction,a two-dimensional Gaussian distribution function was introduced to model the velocity loss at different axial positions in the far wake region based on the assumption of nonlinear wake expansion.This work also developed a“prediction-correction”method to solve the wake velocity field,and the accuracy of the model results was verified in wake experiments on the Garrad Hassan wind turbine.Moreover,a 33-kW two-blade horizontal axis wind turbine was simulated using this method,and the results were compared with the classical wake model under the same parameters and the computational fluid dynamics(CFD)simulation results.The results show that the nonlinear wake model well reflected the influence of incoming flow shear and yaw wake steering in the wake velocity field.Finally,computation of the wake flow for the Horns Rev offshore wind farm with 80 wind turbines showed an error within 8%compared to the experimental values.The established wake model is less computationally intensive than other methods,has a faster calculation speed,and can be used for engineering calculations of the wake velocity in the far wakefield of wind turbines.
基金supported by the National Science Foundation(Grant No.21-36371)supported by the National Science Foundation(Grant Nos.21-38259,21-38286,21-38307,21-37603,and 21-38296)。
文摘Large atmospheric boundary layer fluctuations and smaller turbine-scale vorticity dynamics are separately hypothesized to initiate the wind turbine wake meandering phenomenon,a coherent,dynamic,turbine-scale oscillation of the far wake.Triadic interactions,the mechanism of energy transfers between scales,manifest as triples of wavenumbers or frequencies and can be characterized through bispectral analyses.The bispectrum,which correlates the two frequencies to their sum,is calculated by two recently developed multi-dimensional modal decomposition methods:scale-specific energy transfer method and bispectral mode decomposition.Large-eddy simulation of a utility-scale wind turbine in an atmospheric boundary layer with a broad range of large length-scales is used to acquire instantaneous velocity snapshots.The bispectrum from both methods identifies prominent upwind and wake meandering interactions that create a broad range of energy scales including the wake meandering scale.The coherent kinetic energy associated with the interactions shows strong correlation between upwind scales and wake meandering.
文摘OBJECTIVE The inhibitory effect of active ingredients of Tripterygium wilfordii Hook.F.(TWHF)(celastrol,triptolide,triptonide,wilforlide A,wilforgine and wilforine)on human carboxylester⁃ase 1(CES1)and CES2 was detected to investigate the herb-drug interactions(HDIs)of TWHF.METHODS Human liver microsomes catalysed hydrolysis of 2-(2-benzoyl-3-methoxyphenyl)benzothi⁃azole(BMBT)and fluorescein diacetate(FD)were used as the probe reaction to phenotype the activity of CES1 and CES2,respectively.The residual activities of CES1 and CES2 were detected by ultrahigh performance liquid chromatography(UPLC)after intervention with celastrol,triptolide,triptonide,wilforlide A,wilforgine and wilforine(100μmol·L^(-1)).Kinetics analysis,involving half inhibitory concentra⁃tion(IC_(50)),inhibition type and kinetic parameter(Ki),and in vitro-in vivo extrapolation(IVIVE),was carried out to predict the HDIs between these compounds and CES-metabolizing drugs.Molecular docking was performed to analyze the ligand-enzyme interaction.RESULTS Out of the six main con⁃stituents of TWHF,only celastrol exhibited strong inhibition towards both CES1 and CES2,with the inhibitory rates of 97.45%(P<0.05)and 95.62%(P<0.05),respectively.The IC_(50)was 9.95 and 4.02 mol·L^(-1),respectively,and the types of inhibition were all non-competitive inhibition.Based on the kinetics analysis,the Ki values were calculated to be 5.10 and 10.55μmol·L^(-1)for the inhibition of celastrol on CES1 and CES2,respectively.IVIVE indicated that celastrol might disturb the metabolic hydrolysis of clinical drugs in vivo by inhibiting CES1.Molecular docking results showed that hydrogen bonds and hydrophobic contacts contributed to the interaction of celastrol and CESs.CONCLUSION The inhibitory effect of celastrol on CES1 and CES2 might cause HDIs with clinical drugs hydrolysed by CESs.
基金Baoding Science and Technology Bureau(2141ZF313)。
文摘Objective:To investigate the hook effect in the detection of beta2-microglobulin(β2-MG)with different reagents as well as on two fully automated biochemical analyzers and their solutions.Methods:Using immunoturbidimetric assay forβ2-MG as the research object,β2-MG levels were detected by different concentration gradients ofβ2-microglobulin samples in Liedemann,Mack,and Myriad reagents of three manufacturers on two automatic biochemical analyzers,and the difference of the hook effect was comparatively analyzed.Results:Leadman reagent showed the hook effect on the Beckman AU5800 automated biochemistry analyzer,while both Maccura and Mindray reagents did not show the hook effect.After the experiments,we found the limit value of the pre-zone check of Leadman reagent,and changed the parameters of the instrument,when the limit value of the pre-zone check was reached,the instrument automatically diluted the specimen five times and then detected it again.After changing the parameters of the instrument,the correlation between the three methods of detecting samples of different concentrations was r>0.99.Conclusion:Before selecting the application of immunoturbidimetric reagents,we have to carry out the risk assessment of the hook effect and selectively set the parameters of the pre-zone check based on the highest concentration that may occur in the clinic;for the items that may have the hook effect,we have to selectively set the parameters of the pre-zone check when the ratio of average reaction rate and the immediate reaction rate is at the limit value,and the phenomenon of antigen excess may occur,the instrument will carry out automatic dilution before detection,so as to avoid the issuance of erroneous results of high-value samples due to the hook effect.
文摘The effective utilisation of steel fibre reinforced concrete (SFRC) requires in-depth and detailed understanding of bonding mechanisms governing the tensile behaviour. In response to this demand, this study presents an empirical description of the axial force required to pull out a hooked end steel fibre from a cementitious matrix. It is based upon consistent experimental tests that show the force versus displacement plot is formed from a sequence of events in which partial and full debonding at the interface is followed by bending of the hook knee to raise the force to its maximum. A loss of peak force occurs with the reversed plasticity involved in a full straightening of the fibre that precedes the rapid sliding to its full removal under a falling force. The stages are assembled in the said order with a piecewise connection between linear segments and a curve of exponential decay. A power law can be introduced to describe the knee bending stage if preferred. The normalised co-ordinates were adopted for the simple mathematical discontinuous function of the full pull-out process. Normalising force and displacement for hooked fibre is based upon the maximum values found for straight fibre pull out. This would apply to an overall embedded length of unity and a unit peak force for a full debonding of straight fibre in similar materials. For hooked fibre the normalised co-ordinates given refer to the initiation and duration of each event to be tabulated at fractions of the embedded length with multiples of that force. Such predictions are seen to be in good agreement with average pull-out response curves.
基金financially supported by the National Natural Science Foundation of China (Grant Nos.51909109 and 52101314)the Natural Science Foundation of Jiangsu Province (Grant No.BK20190967)。
文摘Wind farms generally consist of a single turbine installed with the same hub height. As the scale of turbines increases,wake interference between turbines becomes increasingly significant, especially for floating wind turbines(FWT).Some researchers find that wind farms with multiple hub heights could increase the annual energy production(AEP),while previous studies also indicate that wake meandering could increase fatigue loading. This study investigates the wake interaction within a hybrid floating wind farm with multiple hub heights. In this study, FAST.Farm is employed to simulate a hybrid wind farm which consists of four semi-submersible FWTs(5MW and 15MW) with two different hub heights. Three typical wind speeds(below-rated, rated, and over-rated) are considered in this paper to investigate the wake meandering effects on the dynamics of two FWTs. Damage equivalent loads(DEL) of the turbine critical components are computed and analyzed for several arrangements determined by the different spacing of the four turbines. The result shows that the dynamic wake meandering significantly affects downstream turbines’ global loadings and load effects. Differences in DEL show that blade-root flapwise bending moments and mooring fairlead tensions are sensitive to the spacing of the turbines.
文摘SIX thousand kilometers!10,000 li! 3000 yearsold! The only man-madestructure that can be seenfrom the moon! The bricks alonewould build four Egyptian pyra-mids! Wide enough for fourhorses abreast! You are not aman until you’ve been to theWall! We have all heard at leastone of these claims.
文摘China ramps up its efforts to safeguard juveniles from the Internet gaming addiction Just before this year’s national college entrance exam,or the gaokao,on June 7-8,a letter from a despairing mother of student made a big splash on Weibo,a Chinese Twitter-like microblogging platform.The mother described how her son,who used to be an outstanding learner,failed the exam
