拟南芥转录因子HAT1负调控盐胁迫分子机制研究

土壤盐碱化是目前全球农业面临的主要环境挑战之一,为使植物在盐碱地上更好地生长,开展植物盐胁迫响应机制的研究具有重大意义。植物特有的同源结构域-亮氨酸拉链(HD-ZIP)家族转录因子HAT1在拟南芥中调控多种胁迫应答,到目前为止,HAT1是否参与调控植物对盐胁迫的响应尚不清楚。文章围绕转录因子HAT1在拟南芥响应盐胁迫中的调控作用展开研究,证明了EIN3-HAT1-CSDs分子模块可以调控植物对盐胁迫的响应,以期拓展盐胁迫的分子调控网络。盐胁迫诱导EIN3的积累,EIN3抑制HAT1的表达,进而削弱HAT1对下游基因CSD1与CSD2的转录抑制,使抗氧化系统得以激活并清除过量的活性氧,从而增强植株的盐耐受能力。该信号通路为通过分子育种提高植物盐耐受性提供了一个新的潜在靶点。

Chemical Analysis of Activated Carbon from Bull and Cow Horns Pyrolysis to Be Used as Antidotes

The purpose of this study is to compare the results of chemical analysis of two types of activated from the pyrolysis of bull horn and that of cow. Six samples were used to measure pH, carbon, calcium and to determine adsorbent power. The pH was measured at a temperature of 20˚C using an “ANION 7010 ionomer” pH meter, the carbon (C) content was analyzed using a “EURO EA 3000” analyzer. and the electronic balance: “Sartorius CP-2P”, calcium (Ca) was analyzed using a DFS-8 spectrograph. For the adsorbency test, the 0.15% methylene blue R solution was used. At the end of this study, we found that the activated carbon from the bull horn demonstrated a carbon content that is higher than that of the cow horn (20.79% against 15.63%), activated carbon of cow horn is richer in calcium than that of bull horn (16.27% against 3.69%) and then the pH. The cow horn is higher than that of the bull horn (7.43 versus 6.5). For the adsorbent power, the sample (75% bull horn and 25% cow horn) was recorded with the greatest adsorbent power. Thus, from this study, it can be recommended as an activated carbon antidote to be used for poisonings treatment.

Physical and Chemical Properties of Horns Sheaths Particles for the Manufacture of Composite Materials

Salvaged cow horns from slaughterhouses have been transformed into fine particles for a physical characterization that has led us to determine the humidity rate (2.34% ± 0.054%), the actual density situated between 0.586 g/cm3 and 0.732 g/cm3, the swelling rate (12%), and one chemical characterization that permitted us to determine the rate of dry matters (97.05%), of mineral matters (2.5%), of protein matters (94.52%). From these weak values, it can easily be seen that cow horn case doesn’t absorb much water and improve the mechanical characteristics of the composite;the high rate of protein shows that keratin which is the structural molecule favors its gripping as reinforcing element in the manufacturing of composite materials.

Motor neuron-specific RhoA knockout delays degeneration and promotes regeneration of dendrites in spinal ventral horn after brachial plexus injury

Dendrites play irreplaceable roles in the nerve conduction pathway and are vulnerable to various insults.Peripheral axotomy of motor neurons results in the retraction of dendritic arbors,and the dendritic arbor can be re-expanded when reinnervation is allowed.RhoA is a target that regulates the cytoskeleton and promotes neuronal survival and axon regeneration.However,the role of RhoA in dendrite degeneration and regeneration is unknown.In this study,we explored the potential role of RhoA in dendrites.A line of motor neuronal conditional knockout mice was developed by crossbreeding HB9~(Cre+)mice with RhoA~(flox/flox)mice.We established two models for assaying dendrite degeneration and regeneration,in which the brachial plexus was transection or crush injured,respectively.We found that at 28 days after brachial plexus transection,the density,complexity,and structural integrity of dendrites in the ventral horn of the spinal cord of RhoA conditional knockout mice were slightly decreased compared with that in Cre mice.Dendrites underwent degeneration at 7 and 14 days after brachial plexus transection and recovered at 28–56 days.The density,complexity,and structural integrity of dendrites in the ventral horn of the spinal cord of RhoA conditional knockout mice recovered compared with results in Cre mice.These findings suggest that RhoA knockout in motor neurons attenuates dendrite degeneration and promotes dendrite regeneration after peripheral nerve injury.

What Do Bell-Tests Prove? A Detailed Critique of Clauser-Horne-Shimony-Holt Including Counterexamples

Many future directions of scientific endeavors depend on quantum theory and the precise interpretation and significance of the entanglement of quantum-particles. This interpretation depends in turn on the physical meaning of so called Bell-tests that are mostly performed using entangled photons and randomly switched polarizers to measure their polarization at distant locations. This paper presents a detailed critique of the well known theory of Bell tests given by Clauser-Horne-Shimony-Holt (CHSH). It is demonstrated that several important steps of the CHSH derivations contain serious inaccuracies of the underlying physics and probability theory and even a calculus error. As a consequence, the Bell-CHSH theory cannot be used to demonstrate extreme and opposite interpretations of entanglement such as super-luminal influences or alternatively super-determinism that cast aspersions on Einstein’s concepts of locality and separability.

Dynamic physical characteristics of DC arc on arcing horn for HVDC grounding electrode line

The dynamic physical characteristics of a DC arc on an arcing horn for a high voltage direct current(HVDC)grounding electrode line are significantly different from those of the switching device arc,secondary arc,AC fault arc and pantograph-catenary arc.In this work,an experimental platform for the DC arc on the arcing horn was built,and mechanisms of the arc column short circuit and arc root movement were studied.This work further analyzes the characteristics and mechanisms of the arc motion when wind speed and direction,magnetic field and the expansion angle of the electrode are varied.Arc root movement is more likely to occur at the upper electrode.There is a competitive relationship between arc expansion and the transferring effect.The effect of wind on the arc column is greater than the effect on the arc root.The magnetic field has a significant driving effect on both the arc column and the arc root.The research results provide a comprehensive experimental basis for forther probing the method of DC arc suppression,and the improvement of the arcing horn.

Laparoscopic coring-type resection of noncommunicating broadly attached rudimentary horn with recurrent large ovarian endometriomas:A report of two cases

Rudimentary horn of the uterus is a rare congenital malformation that is characterized by significant anatomic variability.We report two cases involving women who presented with a broadly attached rudimentary horn and large ovarian endometriomas that were innovatively treated by laparoscopic coring-type dissection of the rudimentary horn of the uterus.This novel surgical approach ensured the integrity of the myometrium of the unicornuate uterus and avoided uterine rupture during pregnancy and ipsilateral ovarian function impairment.

Medicinal Standard of Rhinoceros Horn and Its Authenticity Identification

Amid the background where the rhinoceros horn has been hoarded as a rare commodity,there is never a lack of counterfeits and adulterated products of rhinoceros horns on the market.This article aims to summarize the commodity specifications and identification key points of the rhinoceros horn from its medicinal standard,to provide a reference for studying substitutes of rhinoceros horn.

Application of"Six Thinking Hats"in the Investigation and Improvement of Hidden Dangers in Food Safety

With the development of economy,people's living standards are constantly improving,and the requirements for food safety are getting higher and higher.The Food Safety Law stipulates that enterprises should implement the main responsibility of food safety,and the investigation and improvement of food safety hazards plays an important role in improving the food safety management level of enterprises and reducing food safety risks.This paper combines the innovative thinking mode of six thinking hats with food safety,discusses the application mode of six thinking hats in food safety investigation and improvement,and hopes to improve food safety level through this way.

影响和牛冷冻胚胎移植妊娠率的因素分析

为研究不同因素对和牛冷冻胚胎移植妊娠率的影响,采用Ovsynch方法对受体荷斯坦牛进行同期发情,共计移植受体557头。结果表明,A级和B级胚胎的移植妊娠率(57.72%,56.63%)差异不显著(P>0.05);不同黄体直径CL-1(<1.0 cm)、CL-2(1.0~1.5 cm)和CL-3(>1.5 cm)时的移植妊娠率(54.50%,59.72%和55.24%)差异也不显著(P>0.05);左侧和右侧子宫角移植妊娠率(56.92%,57.41%)差异不显著(P>0.05);子宫角前段移植妊娠率(59.04%)显著高于子宫角中段移植妊娠率(40.35%)(P<0.05);不同移植完成时间T-1(6~9 min)和T-2(10~13 min)内完成的移植妊娠率(60.65%,54.17%)显著高于T-3(14~24 min)内完成的移植妊娠率(26.67%)(P<0.05)。说明胚胎质量、移植部位和黄体直径对胚胎移植妊娠率均无显著影响,胚胎输送位置和移植完成时间对胚胎移植妊娠率有显著影响。

宫角异位型侵袭性葡萄胎一例

本文报道了一例异位侵袭性葡萄胎患者的临床特征、诊断过程及治疗方案。其人绒毛膜促性腺激素(β-HCG)提示24335 m IU/mL、超声提示左侧附件区异常回声(不除外滋养细胞疾病)及病检示(宫内组织)分泌期样宫内膜,左输卵管及左宫角、左阔韧带病灶查见水肿的绒毛组织并伴有滋养叶细胞增生,多系葡萄胎;免疫组化提示p53(+)、p57(-)、HCG(+),人胎盘碱性磷酸酶(PLAP)(-)、Ki-67(高表达),组织学类型符合完全性葡萄胎,CD34及弹力纤维染色提示一处血管内查见绒毛组织,符合侵袭性葡萄胎改变,确诊为侵袭性葡萄胎。治疗上采用了以手术为主、辅助化疗的综合治疗策略。

残角子宫妊娠至34+周合并胎盘植入1例分析

目的探讨残角子宫妊娠的妊娠机制、临床诊断及处理,分析误诊原因。方法总结甘肃省妇幼保健院产科2021年7月收治的1例残角子宫妊娠至34+周并发胎盘植入病人的病史资料及诊疗方案,做文献复习。结果孕妇主诉停经34^(+)周,产检发现前置胎盘5 d。入院主要诊断“残角子宫妊娠,胎盘植入,孕34^(+)周,孕2产1”。入院后完善相关检查,行手术治疗,术中明确残角子宫妊娠合并胎盘植入,娩出胎儿后,切除残角子宫。分析误诊原因:未定期检查,中孕期未能分辨宫腔与宫颈的关系,病人有经阴道分娩史,故误诊为正常宫腔妊娠。中孕期检查如发现妊娠子宫位置较高,应充分扫查盆腔有无单角子宫存在,妊娠子宫与宫颈有无相连,有助于残角子宫妊娠漏诊的发现,MRI辅助检查在中晚孕期能提高残角子宫妊娠诊断的准确率。结论孕期需要加强监测及管理,仔细询问病史,详细专科查体,提高早期诊断率,降低母婴不良事件的发生。

单脉冲平顶激光清洗CFRP的多物理场特性研究

为探究平顶激光清洗碳纤维增强复合材料(CFRP)的作用机制,本研究考虑CFRP的组分对激光的吸收、反射特性差异显著,将激光载荷分层施加到CFRP的细观有限元模型上,引入热烧蚀-热应力双重作用效果,还原不同能量密度下单脉冲平顶激光清洗形貌,并完成了试验验证。结果表明:分层施加激光载荷较传统表面加载模式能够有效提升仿真精度,采用热烧蚀-热应力双重判据能够进一步提升形貌还原效果,本研究模型的仿真形貌长轴贴合度不低于96.64%,面积贴合度达到80.00%,并通过高精度模型瞬时温度场分布能够指导激光清洗CFRP表面树脂的实际工艺。

腹腔镜下宫角妊娠改良荷包缝扎法11例疗效分析

目的:探索腹腔镜下改良的宫角妊娠荷包缝扎法用于止血预处理。方法:收集2022年2月-2023年2月于本院因宫角妊娠在腹腔镜下行改良荷包缝扎法的11例患者临床资料,分析手术治疗效果。结果:11例术中出血5~100ml,手术时间35~90min,无一例发生副损伤及紧急中转开腹。术后6例因血hCG下降满意未口服米非司酮,5例单次口服200mg米非司酮片1片巩固治疗。11例血清β-hCG于术后1月内均降至正常,术后月经均正常。结论:改良的腹腔镜下宫角妊娠荷包缝扎法安全、有效,值得临床应用。

一种新型可移动TEM喇叭辐射波模拟器的模拟优化

为改善基于TEM喇叭的辐射波模拟器的低频辐射特性从而展宽其辐射近场半高宽,首次提出了在指数型TEM喇叭上/下两个极板的端口加上2个金属直板、并通过倾斜金属板和并联电阻相连的新型可移动模拟器的设计方案。基于FDTD方法模拟分析了该新型模拟器的特性参数对其近场辐射性能的影响,并给出了优化后的模拟器及其阵列的辐射特性。计算结果表明:尺寸为6 m×6 m×6.24 m的优化后的新型模拟器在距离口面3 m的中心位置的辐射近场脉宽能达到18.95 ns,而达到相同低频辐射性能的常规模拟器尺寸为9 m×12 m×6.8 m。且与常规模拟器相比,优化后的模拟器的场峰值更大。与前人的研究相比,优化后的模拟器场在保持高峰值的同时,时域波形后延震荡的幅度与主峰的比值明显减小。优化后的模拟器2×2阵列模型的测试平面中心点场峰值最大,且在测试平面上满足6 dB均匀性要求的有效测试区最大;有效测试区在横向上范围最大的是2×2阵列模型,其次是2×1阵列模型;在纵向上范围最大的是2×2阵列模型及1×2阵列模型。

电针对紫杉醇诱导大鼠神经病理性疼痛的影响

目的观察电针(EA)对紫杉醇诱导神经病理性疼痛大鼠脊髓背角NKCC1、KCC2表达和小胶质细胞活化的影响及其可能机制。方法将48只雄性SD大鼠随机分为溶媒组(Vehicle组)、紫杉醇组(PTX组)、紫杉醇+电针组(PTX+EA组)、紫杉醇+假电针组(PTX+Sham EA组),每组12只。采用腹腔注射PTX的方法建立PTX诱导的神经病理性疼痛大鼠动物模型,建模完成后,PTX+EA组给予“足三里”、“阳陵泉”电针刺激,连续7 d。于紫杉醇注射前2 d和注射后第1、3、5、7、14、21天进行机械撤足阈值和热缩足潜伏期痛行为学测试。利用免疫荧光染色技术和Western blot技术检测脊髓背角组织中钠钾氯联合转运蛋白1(NKCC1)、钾氯联合转运蛋白2(KCC2)和小胶质细胞标志物离子钙结合衔接分子1(Iba1)表达的变化。结果与Vehicle组比较,PTX组大鼠出现双后足机械和热痛觉过敏,脊髓背角组织中NKCC1表达升高和活化的小胶质细胞数增加。与PTX组比较,PTX+EA组大鼠在第14、21天的机械和热痛觉过敏得到显著改善,脊髓背角组织中NKCC1和Iba1表达量降低,4组间KCC2表达差异无统计学意义。结论电针可有效缓解紫杉醇诱导的神经病理性疼痛,其机制可能与抑制大鼠脊髓背角组织中NKCC1表达和小胶质细胞活化有关。

冲出重“唯”:学术资本视域下高校教师评价改革的困境、症结与出路

随着我国高校教师评价改革持续推进,破“唯”工作初见成效,但“唯帽子”的顽瘴痼疾仍需整治,“唯项目”也成为破“五唯”的非意图性后果。人才称号和科研项目作为学术资本,在不断累积与转换的过程中逐渐产生异化,对科技人才队伍建设、科技创新能力培育、内生创新动力激发、科研价值取向塑造等方面造成了一定的负面影响。其中,学术资本生成的塑型作用是诱发因素,学术资本转化的放大作用是核心因素,学术资本垄断的聚集作用是增强因素。推动高校教师评价冲出重“唯”,需要拓展学术资本生成路径、扭转学术资本转化逻辑、打破学术资本垄断怪圈,通过落实多元分类评价、强化科研环境支撑、释放学术共同体活力,构建更加有利于高校教师发展的学术评价生态。

基于米特-霍恩模型的高校实验室安全管理制度执行困境及路径优化

为提高高校实验室安全管理规范相关文件的执行效果,以国家教育部、省教育厅、某高校发布的实验室安全管理相关制度为对象,引入米特-霍恩模型分析高校实验室安全管理制度的执行路径,并运用文本分析法寻找文件中与其作用因素相关的关键词,进行制度执行过程中的困境分析。研究结果表明,在实验室安全管理方面,高校可能会面临多重困境,包括实验室危险因素的多样化、实验室安全经费的不足、制度执行主体之间的交流分层化、职能重叠、制度执行者缺乏足够的实施驱动力等问题。文章对实验室安全管理制度执行进行了路径优化,旨在健全我国高校实验室安全监管机制,降低高校实验室事故发生率。

“六顶思考帽”思维模式在护生岗位胜任力培养中的应用

目的:研究“六顶思考帽”在护理岗位胜任力培养的教学成效,并对其教学效果进行评价。方法:选取病区内40名护生为研究对象,采用“六顶思考帽”思维模式进行岗位能力培养。结果:该模式和传统教学方法比较,能明显提升护生的心理特质、协作沟通能力、评判性思维能力、临床护理能力和职业发展能力等岗位胜任力,同时提高教学的满意度,差异均有统计学意义(P<0.05)。结论:“六顶思考帽”思维模式能显著提高护生岗位胜任力和教学满意度,是提升护生岗位能力,改善临床教学质量的一种可行方法。

非遗视域下新丰乡唢呐的传承与发展研究

随着时代的变迁和社会的发展,非物质文化遗产的保护与传承日益受到重视。新丰乡唢呐作为一项具有深厚地域文化内涵和独特艺术价值的民间音乐形式,面临着传承与发展的诸多挑战。文章以非遗视域为出发点,通过田野调查与历史文献研究,对新丰乡唢呐的历史渊源、艺术特征、传承现状进行了全面梳理。研究发现,新丰乡唢呐传承面临一系列问题,如传承人群老龄化、民众对其兴趣缺失、传统曲目大量流失、创新能力不足、相关机制不完善、资金匮乏以及现代生活方式的冲击。针对这些问题,提出了包括强化传承人培养、曲目创新、建立传承体系、促进文化交流等一系列切实可行的策略。