Development characteristics and controlling factors of fractures in lacustrine shale and their geological significance for evaluating shale oil sweet spots in the third member of the Shahejie Formation in the Qikou Sag, Bohai Bay Basin

Natural fractures are critical for shale oil and gas enrichment and development. Due to the extremely high heterogeneity of shale, the factors controlling the formation of internal fractures, especially horizontal fractures, remain controversial. In this study, we integrate thin section analysis and microcomputed tomography(CT) data from several lacustrine shale samples from the third member(Es3) of the Shahejie Formation, Qikou Sag, Bohai Bay Basin, to assess the fractures in detail. The goal is to reveal the development characteristics, controlling factors, and geological significance for evaluating sweet spots in a shale oil play. The fractures in the Es3contain high-angle structural and horizontal bed-parallel fractures that are mostly shear and extensional. Various factors influence fracture development,including lithofacies, mineral composition, organic matter content, and the number of laminae. Structural fractures occur predominantly in siltstone, whereas bed-parallel fractures are abundant in laminated shale and layered mudstone. A higher quartz content results in higher shale brittleness, causing fractures, whereas the transformation between clay minerals contributes to the development of bedparallel fractures. Excess pore pressure due to hydrocarbon generation and expulsion during thermal advance can cause the formation of bed-parallel fractures. The density of the bed-parallel and structural fractures increases with the lamina density, and the bed-parallel fractures are more sensitive to the number of laminae. The fractures are critical storage spaces and flow conduits and are indicative of sweet spots. The laminated shale in the Es3with a high organic matter content contains natural fractures and is an organic-rich, liquid-rich, self-sourced shale play. Conversely, the siltstone, massive mudstone, and argillaceous carbonate lithofacies contain lower amounts of organic matter and do not have bed-parallel fractures. However, good reservoirs can form in these areas when structural fractures are present and the source, and storage spaces are separated.

Key geological factors governing sweet spots in the Wufeng-Longmaxi shales of the Sichuan Basin,China

Production performance of the Wufeng-Longmaxi shales varies significantly among Fuling,Weirong,and Wulong fields in the Sichuan Basin.Total organic carbon(TOC)content,mineralogy,and organic matter(OM)pore characteristics are investigated to identify key factors governing sweet spots.Siliceous shales with good preservation conditions in the Fuling Field exhibit large thickness,high TOC content and thin-section porosity(TSP),and well-developed OM macropores,thus high initial production and estimated ultimate recovery(EUR).Thin carbonate-containing siliceous shales with good preservation conditions in the Weirong Field feature medium-to-high TOC and well-developed OM macropores but low TSP,leading to high initial production but low EUR.Siliceous shales with poor preservation conditions in the Wulong Field are characterized by large thickness,high TOC,low TSP and poorly-developed OM macropores,causing low initial production and EUR.Both sedimentary and preservation conditions are intrinsic decisive factors of sweet spots,as they control the mineral composition,TOC,and OM macropore development.Deep-water shales in transgressive systems tracts(TSTs)exhibit better-developed OM macropores and greater TOC compared to highstand systems tracts(HSTs).OM macropores are most prevalent in siliceous shales,followed by carbonate-containing siliceous shales and then argillaceous shales.Furthermore,good preservation conditions are conducive to retain OM macropores with low pore aspect ratio(PAR).Comparison among the three fields shows that high-TOC silicious shales with good preservation conditions are the highest in TSP and EUR.Therefore,organic richness,lithofacies,and preservation conditions are the major factors which determine OM pore development,governing the sweet spots of the Wufeng-Longmaxi shales.

Prestack inversion based on anisotropic Markov random field-maximum posterior probability inversion and its application to identify shale gas sweet spots

Economic shale gas production requires hydraulic fracture stimulation to increase the formation permeability. Hydraulic fracturing strongly depends on geomechanical parameters such as Young＇s modulus and Poisson＇s ratio. Fracture-prone sweet spots can be predicted by prestack inversion, which is an ill-posed problem; thus, regularization is needed to obtain unique and stable solutions. To characterize gas-bearing shale sedimentary bodies, elastic parameter variations are regarded as an anisotropic Markov random field. Bayesian statistics are adopted for transforming prestack inversion to the maximum posterior probability. Two energy functions for the lateral and vertical directions are used to describe the distribution, and the expectation-maximization algorithm is used to estimate the hyperparameters of the prior probability of elastic parameters. Finally, the inversion yields clear geological boundaries, high vertical resolution, and reasonable lateral continuity using the conjugate gradient method to minimize the objective function. Antinoise and imaging ability of the method were tested using synthetic and real data.

Intelligent prediction and integral analysis of shale oil and gas sweet spots

Shale reservoirs are characterized by low porosity and strong anisotropy. Conventional geophysical methods are far from perfect when it comes to the prediction of shale sweet spot locations, and even less reliable when attempting to delineate unconventional features of shale oil and gas. Based on some mathematical algorithms such as fuzzy mathematics, machine learning and multiple regression analysis, an effective workflow is proposed to allow intelligent prediction of sweet spots and comprehensive quantitative characterization of shale oil and gas reservoirs. This workflow can effectively combine multi-scale and multi-disciplinary data such as geology, well drilling, logging and seismic data. Following the maximum subordination and attribute optimization principle, we establish a machine learning model by adopting the support vector machine method to arrive at multi-attribute prediction of reservoir sweet spot location. Additionally, multiple regression analysis technology is applied to quantitatively predict a number of sweet spot attributes. The practical application of these methods to areas of interest shows high accuracy of sweet spot prediction, indicating that it is a good approach for describing the distribution of high-quality regions within shale reservoirs. Based on these sweet spot attributes, quantitative characterization of unconventional reservoirs can provide a reliable evaluation of shale reservoir potential.

Application of Pore Evolution and Fracture Development Coupled Models in the Prediction of Reservoir "Sweet Spots" in Tight Sandstones

The Chang-63 reservoir in the Huaqing area has widely developed tight sandstone ＂thick sand layers, but not reservoirs characterized by rich in oil＂, and it is thus necessary to further study its oil and gas enrichment law. This study builds porosity and fracture development and evolution models in different deposition environments, through core observation, casting thin section, SEM, porosity and permeability analysis, burial history analysis, and ＂four-property-relationships＂ analysis.

Evaluation of sweet spots and horizontal-well-design technology for shale gas in the basin-margin transition zone of southeastern Chongqing,SW China

The shale gas accumulation conditions in the basin-margin transition zone of southeastern Chongqing in SW China are complex.In order to improve single-well productivity in this area,the geologic characteristics,major factors controlling the occurrence of sweet spots,and drilling/fracturing optimization were investigated in this study.The sweet spot evaluation system and criteria were established,and the horizontal-well-design technology was developed.The following three conclusions were drawn.First,the accumulation and high-productivity-oriented approaches for sweet spot evaluation are proposed and the criteria are established based on screened key indicators.Second,the horizontal well was designed based on:(1)the“six-map”method,to identify both the geology and engineering sweet spots for well locations;and(2)seismic attributes,to predict the development of fractures and cavities,and thus,avoid mud loss and improve the drilling efficiency.The target window,well-azimuth optimization,and the curvature were forecasted to improve the fracturing performances.Third,the Pingqiao anticline,Dongsheng anticline,Jinfo slope,and Wulong syncline were selected as Type I sweet spots.Currently,shale gas has been successfully discovered in the basin-margin transition zone and is being commercially developed.

Quantitative prediction of shale gas sweet spots based on seismic data in Lower Silurian Longmaxi Formation,Weiyuan area,Sichuan Basin,SW China

Sweet spots in the shale reservoirs of the Lower Silurian Longmaxi Formation in Weiyuan 201 Block of Sichuan Basin were predicted quantitatively using seismic data and fuzzy optimization method. First, based on seismic and rock physics analysis, the rock physics characteristics of the reservoirs were determined, and elastic parameters sensitive to shale reservoirs with high gas content were selected. Second, data volumes with high precision of the elastic parameters were obtained from pre-stack simultaneous inversion. The horizontal distribution of key parameters for shale gas evaluation were calculated based on the results of rock physics analysis. Then, the fuzzy evaluation equation was established by fuzzy optimization method with test and logging data of horizontal wells with similar operation conditions. key parameters affecting the productivity of horizontal wells were sorted out and the weights of them in the sweet spots quantitative prediction were worked out by fuzzy optimization to set up a sweet spots evaluation system. Three classes of shale gas reservoirs which including two kinds of sweet spots were predicted with the above procedure, and the sweet spots have been predicted quantitatively by combining the above prediction results with the testing production. The testing results of 7 verification wells proved the reliability of the prediction results.

Research status and hotspots of tight junctions and colorectal cancer:A bibliometric and visualization analysis

BACKGROUND Colorectal cancer(CRC)is the third most common cancer worldwide and the second leading cause of cancer-related death.Over the past two decades,numerous researchers have provided important evidence regarding the role of tight junction(TJ)proteins in the occurrence and progression of CRC.The causal relationship between the presence of specific TJ proteins and the development of CRC has also been confirmed.Despite the large number of publications in this field,a bibliometric study to review the current state of research and highlight the research trends and hotspots in this field has not yet been performed.AIM To analyze research on TJs and CRC,summarize the field’s history and current status,and predict future research directions.METHODS We searched the Science Citation Index Expanded database for all literature on CRC and TJs from 2001-2023.We used bibliometrics to analyze the data of these papers,such as the authors,countries,institutions,and references.Co-authorship,co-citation,and co-occurrence analyses were the main methods of analysis.CiteSpace and VOSviewer were used to visualize the results.RESULTS A total of 205 studies were ultimately identified.The number of publications on this topic has steadily increased since 2007.China and the United States have made the largest contributions to this field.Anticancer Research was the most prolific journal,publishing 8 articles,while the journal Oncogene had the highest average citation rate(68.33).Professor Dhawan P was the most prolific and cited author in this field.Co-occurrence analysis of keywords revealed that“tight junction protein expression”,“colorectal cancer”,“intestinal microbiota”,and“inflammatory bowel disease”had the highest frequency of occurrence,revealing the research hotspots and trends in this field.CONCLUSION This bibliometric analysis evaluated the scope and trends of TJ proteins in CRC,providing valuable research perspectives and future directions for studying the connection between the two.It is recommended to focus on emerging research hotspots,such as the correlations among intestinal microbiota,inflammatory bowel disease,TJ protein expression,and CRC.

Geological and Engineering‘Sweet Spots'in the Permian Lucaogou Formation,Jimusar Sag,Junggar Basin

Unconventional oil and gas resources require petrophysical logs to answer the question of how best to optimize geological and engineering‘sweet spots'.Therefore,the establishment of a key well with comprehensive descriptions of lithology,reservoir properties,hydrocarbon-bearing properties,electronic well log responses,source rock properties,brittleness,and in situ stress magnitude and direction is important for the effective exploration and production of unconventional hydrocarbon resources.Cores,thin sections,scanning electron microscopy(SEM)and comprehensive well log suites are used to build a key well for the Permian Lucaogou Formation,Jimusar Sag of the Junggar Basin.The results show that there are three main types of lithologies,including siltstone,mudstone and dolostone.Lithologies can be predicted using the combination of conventional well and image logs.The pore spaces consist of interparticle pores,intragranular dissolution pores and micropores.Nuclear Magnetic Resonance(NMR)T_(2)components longer than 1.7 ms are superposed as effective porosity.Permeability is calculated using the Coates model from NMR T_(2)spectra.The ratio of T_(2)components>7.0 ms to T_(2)components>0.3 ms is used to calculate oil saturation.TOC is calculated using theΔlog R method.Brittleness index is calculated using Poisson-Young's method,ranging from 13.42%-70.53%.In situ stress direction is determined,and in situ stress magnitudes(maximum horizontal stress SH_(max),minimum horizontal stress Sh_(min),vertical stress S_(v))are calculated using density and sonic logs.The strike-slip stress type(SH_(max)>S_(v)>Sh_(min))is encountered.The key well which comprehensively includes the above seven properties is established.Geological and engineering(geomechanical)‘sweet spots'are then optimized from the key well by fully analyzing lithology,reservoir property,oilbearing potential,in situ stress magnitude and brittleness.It is hoped that the results support engineers'and geologists'decisions for the future exploitation of unconventional hydrocarbon resources.

Comprehensive Evaluation of Geological and Engineering Sweet Spots of Shale Gas Reservoir: A Case Study of the Luzhou Block, Sichuan Basin

There is a huge amount of marine shale gas resources in the southern Sichuan Basin in China, and most of the resources are at the buried depth of 3500 - 4500 meters. At present, deep shale gas is in the early stage of exploration and development. In order to achieve large-scale efficient development, in addition to optimizing favorable blocks, it is also to identify the optimal target in the vertical direction combine geology, drilling, and fracturing. Therefore, Taking the Longmaxi formation shale in the Luzhou block as the research object, based on drilling, logging, and core experiment data, through single well and 3D geomechanical modeling methods, analyze the characteristics of organic matter abundance, porosity, pore pressure, collapse pressure, mineral composition and in-situ stress of different layers of shale in Longmaxi formation. Systematically summarized the main controlling factors of the “sweet spot” of deep shale gas and establish the comprehensive evaluation system of deep shale gas “sweet spots”, to clarify the optimal “sweet spots” of geology, drilling, and fracturing in the Longmaxi reservoir. Results show that the total organic carbon content, porosity, and gas saturation of the long111 layer are higher than other layers. The Long111 layer has a low collapse pressure and a high compressive strength, the risk of wellbore instability is relatively low. The stress difference coefficient of All layers is less than 0.3, and the brittleness index of the Long111 layer is 62.35%. A complex fracture network is easier to form after fracturing. The conclusion shows that the Long111 layer is the optimal reservoir section of the Longmaxi Formation. Ensure the drilled rate of the Long111 layer and maximize the length of the horizontal section can obtain higher production.

Quantitative and Comprehensive Prediction of Shale Oil Sweet Spots in Qingshankou Formation, Songliao Basin

The mud shale of Qingshankou Formation in Songliao Basin is the main rock source and contains rich shale oil resources. The successful development of shale oil depends on evaluating and optimizing the “sweet spots”. To accurately identify and optimize the favorable sweet spots of shale oil in Qingshankou Formation, Songliao Basin, the original logging data were preprocessed in this paper. Then the thin mud shale interlayer of Qingshankou Formation was identified effectively by using the processed logging data. Based on the artificial neural network method, the mineral content of mud shale in Qingshankou Formation was predicted. The lithofacies were identified according to the mineral and TOC content. Finally, a three-dimensional (3-D) model of total organic carbon (TOC), vitrinite reflectance (Ro), mineral content, and rock of Qingshankou Formation in Songliao Basin was established to evaluate and predict the favorable sweet spots of shale oil in the study area. The results show that there are a lot of calcareous and siliceous thin interlayers in Qingshankou Formation, and TOC content is generally between 2% and 3%. Ro is the highest in Gulong sag, followed by Sanzhao sag. The lithofacies mainly consists of felsic shale and mixed shale, mainly in the first member of Qingshankou Formation. Comprehensive analysis shows that shale oil development potential is enormous in the eastern part of Sanzhao Sag and the northern part of Gulong Sag.

Multi-source genesis of continental carbonate-rich fine-grained sedimentary rocks and hydrocarbon sweet spots

This paper systematically discusses the multiple source characteristics and formation mechanisms of carbonate-rich fine-grained sedimentary rocks through the analysis of material source and rock formation.The hydrocarbon accumulation characteristics of carbonate-rich fine-grained sedimentary rocks are also summarized.The results show that the main reason for the enrichment of fine-grained carbonate materials in rift lake basins was the supply of multiple material sources,including terrestrial material input,formation of intrabasinal authigenic carbonate,volcanic-hydrothermal material feeding and mixed source.The development of carbonate bedrock in the provenance area controlled the filling scale of carbonate materials in rift lake basins.The volcanic-hydrothermal activity might provide an alkaline fluid to the lake basins to strengthen the material supply for the formation of carbonate crystals.Authigenic carbonate crystals induced by biological processes were the main source of long-term accumulation of fine-grained carbonate materials in the lake basins.Carbonate-rich fine-grained sedimentary rocks with multiple features were formed through the interaction of physical,biochemical and chemical processes during the deposition and post-deposition stages.The source and sedimentary origin of the fine-grained carbonate rock controlled the hydrocarbon accumulation in it.In the multi-source system,the types of"sweet spots"of continental shale oil and gas include endogenous type,terrigenous type,volcanic-hydrothermal type and mixed source type.

Analysis of tight oil accumulation conditions and prediction of sweet spots in Ordos Basin: A case study

Tight sandstone reservoirs are widely developed in the Mesozoic Yanchang Formation of the Ordos Basin,China.There is a lack of understanding on the sedimentary setting,source-reservoir relationship and oil accumulation conditions in this area.In this study,through the comprehensive analysis of the distri-bution of tight oil,we evaluated the properties and petrological features of reservoir,geochemical characteristics of source rocks,the source-reservoir relationship,as well as the trapping,preservation and accumulation conditions of tight oil in the Chang 7 Member,and predicted the sweet spots of tight oil in the study area.The results show that the Chang 7 Member is a typical low-porosity and ultra-low permeability reservoir with great tightness,small pore throat and high capillary pressure,and must have been of near-source accumulation.The source rocks are mainly developed in the Chang 7_(3) submember,and the reservoirs mainly occur in the Chang 7_(1) and Chang 7_(2) submembers,forming a combination mode of“lower source rock and upper reservoir”.Sandbodies with good connectivity and fractures being well developed in local areas are the main hydrocarbon transport systems.The abnormal high pressure caused by hydrocarbon generation and pressurization is the main driving force of tight oil accumulation.The mode of hydrocarbon transportation is dominated by the vertical or lateral migration from under-lying source rocks or adjacent source rocks to reservoirs within a short distance.Following the integrated evaluation of lithology,physical properties and oil saturation of reservoirs and geochemical character-istics of source rocks,we grouped the sweet spots of Chang 7 Member into three types:Type I,Type II and Type III.Among others,the Type I sweet spots are the best in terms of porosity,permeability and source rock thickness and hydrocarbon enrichment which should be the focus of oilfield development.This study lays an important foundation for the economic and efficient development of tight oil in the Chang 7 Member of Heshui area,and has important implications on tight sandstone reservoirs in other regions of Ordos Basin in China.

Types and genesis of sweet spots in the tight sandstone gas reservoirs:Insights from the Xujiahe Formation,northern Sichuan Basin,China

Through comprehensively applying geological and geophysical data,as well as core and thin section observation,the characteristics of reservoirs and fractures in the second member of the Xujiahe Formation(hereinafter referred to as Xu2 Member)in the Yuanba area,northern Sichuan Basin,were studied.Combined with the analysis of the main controlling factors of production capacity,the types and characteristics of the sweet spots in the tight sandstone gas reservoir were determined.The evaluation standards and geological models of the sweet spots were established.The results are as follows:(1)There are bedding-parallel fracture-,fault-induced fracture-,and pore-dominated sweet spots in the tight sandstone gas reservoirs of the Xu2 Member.(2)The bedding parallel fracture-dominated sweet spots have developed in quartz sandstones with well-developed horizontal fractures and micro-fractures.They are characterized by high permeability and high gas output during production tests.This kind of sweet spots is thin and shows a limited distribution.Their logging responses show extremely low gamma-ray(GR)values and medium-high AC values.Moreover,the bedding parallel fracture-dominated sweet spots can be mapped using seismic methods.(3)The fault-induced fracture-dominated sweet spots have welldeveloped medium-and high-angle shear fractures.Their logging responses show an increase in peaks of AC values and total hydrocarbon content and a decrease in resistivity.Seismically,the areas with welldeveloped fault-induced fracture-dominated sweet spots can be effectively mapped using the properties such as seismic entropy and maximum likelihood.(4)The pore-dominated sweet spots are developed in medium-grained feldspathic litharenites with good reservoir properties.They are thick and widely distributed.(5)These three types of sweet spots are mainly determined by sedimentation,diagenesis,and tectonism.The bedding parallel fracture-dominated sweet spots are distributed in beachbar quartz sandstones on the top of the 1st sand layer group in the Xu2 Member,which develops in a shore-shallow lake environment.The fault-induced fracture-dominated sweet spots mainly occur near faults.They are increasingly developed in areas closer to faults.The pore-dominated sweet spots are primarily distributed in the 2nd and 3rd sand layer groups,which lie in the development areas of distributary channels near provenances at western Yuanba area.Based on the geological and seismic data,a comprehensive evaluation standard for these three types of sweet spots of the tight sandstone reservoirs in the Xu2 Member has been established,which,on the one hand,lays the foundation for the development and evaluation of the gas reservoir,and on the other hand,deepens the understanding of sweet spot in the tight sandstone gas reservoirs.

Production characteristics and sweet-spots mapping of the Upper Devonian-Lower Mississippian Bakken Formation tight oil in southeastern Saskatchewan, Canada

A workflow that helps identify potential production sweet spots in the Middle Bakken tight oil play is proposed based on analysis of large amounts of production data. The proposed approach is a multivariate statistical model that extracts relevant information from a training dataset of production wells to facilitate geological similarity comparison between economic and sub-economic production wells. The model is applied to the Middle Bakken tight oil play in southeastern Saskatchewan. Data screening for diagnostic geological indicators for sweet spots reveals that several geological factors indicative for conventional oil reservoirs seem to work for the Middle Bakken tight oil play as well. These factors include: a) the NE Torqunay-Rocanville Trend serving as a preferred regional migration path for connecting mature source rock in southern Williston Basin and the Middle Bakken tight reservoir in southeastern Saskatchewan; b) the oils in the Bakken tight reservoirs along the U.S. and Canada border are more likely from local matured Bakken source rocks; c) subtle structural components enhancing the convergence of dispersed hydrocarbons over a large area; d) top seal and lateral barrier improving preservation, thus favouring oil productivity; e) orientation of maximum horizontal stress coincident with the direction of the variogram spatial continuity in ultimate recoverable reserves, so the direction of horizontal well has a significant impact on the oil productivity.

Cultivation and Management of Low-carbon Scenic Spots——A case study of Yuntai Mountain Scenic Area in Henan Provicne of China

After analysis of connotation of low-carbon scenic spots,the paper considered that a perfect low-carbon scenic spot is a comprehensive integrity with satisfying tourists' lowest tourist experience effect as the premise and with low energy consumption,low pollution and low emission as the foundation.Low-carbon tourist scenic spots had been classified into three types which are ecological type,cultural type and coupling type of ecology and culture.Representatives of each type of tourist scenic spot were illustrated.It proposed that government should play the leading role in cultivation of low-carbon scenic spot;tourism industry should enhance self supervision;tourism development mode should be transformed and upgrading of tourist products should be realized.By taking Yuntai Mountain Scenic Area of Henan Province for example,based on low-carbon tourist resources,low-carbon development of management,following measures realizing low-carbon economy were put forward:① purifying air quality and beautifying environment of scenic area;② sifting laggard productivity and protecting ecological resources in the scenic area by many means;③ sticking to low-carbon environmental protection concept and completing infrastructure service;④ developing tourist native products and souvenirs based on local resources;⑤ applying electronic access control ticket system,intelligent monitor system and LED information and communication platform,so as to achieve low-carbon management.

Research on the Value of Chinese Famous Mountainous Scenic Spots in the Perspective of Cultural Landscape

After summarizing relevant researches on famous mountainous scenic spots and the development of cultural landscapes in world cultural heritages, the paper has analyzed the value of famous mountainous scenic spots in China and considered that Chinese famous mountainous scenic spots are of rich natural science value and unique aesthetic value, have inherited traditional humane landscape aesthetic value, reflected the philosophical view of "the unity between heaven and human" and profound religious culture, and preserved rich cultural remains, corresponding with the definition and requirement of cultural landscape in world heritages.

STUDY ON TURBULENT SPOTS IN PLANE COUETTE FLOW

Turbulent spots play a key role in the formation of the turbulence and the transition. The generation and evolution of turbulent spots using the wall impulse model in the plane Couette flow are studied by direct numerical simulation of Navier-Stokes equations. A group of three-dimensional coupling compact difference schemes with high accuracy and high resolution is used in the numerical calculation. The important characteristics of turbulent spots based on the results of examples are analyzed, including the formation of random pulse, the generation of Reynolds stress, the growth of disturbance amplitude, and the continuous change of spot shape, especially the complex evolution process of the streamwise vortices. Computational results confirm that basic properties of turbulent spots in the laminar flow are similar to those in the turbulent flow.

枸杞SWEET基因家族鉴定及其与可溶性糖含量关系

【目的】糖外排转运蛋白(sugars will eventually be exported transporters,SWEETs)在植物生长发育过程中发挥重要作用,解析SWEETs基因在枸杞果实发育过程中对糖积累作用,为进一步揭示SWEETs基因在枸杞果实发育过程中的作用提供参考。【方法】用生物信息学方法对枸杞SWEET基因(LbaSWEETs)进行全基因组鉴定,并用已发表的转录数据分析LbaSWEETs在果实发育时期的基因表达情况。【结果】枸杞SWEET基因家族共有37个成员,随机分布于10条染色体上,分别编码152~621个氨基酸,蛋白质分子质量为16.87~69.97 kD,等电点为4.96~9.86。亚细胞定位预测位于叶绿体或质膜,大多数含有7个跨膜螺旋。系统进化分析发现,37个LbaSWEETs蛋白可分为4个亚群,每个亚群的基因结构和保守基序组成相似。启动子元件分析表明:Lba-SWEETs基因启动子富含大量激素响应、逆境胁迫和生长发育响应元件。转录组数据和qRT-PCR分析表明:LbaSWEET9和LbaSWEET29基因表达量随果实成熟呈现显著增加。相关性分析结果表明,LbaSWEET9和LbaSWEET29基因表达量与果糖含量呈显著正相关。【结论】LbaSWEET9和LbaSWEET29基因是果糖积累的关键基因。

基于转录组信息的百合SWEET基因家族鉴定及表达特性分析

以东方百合品种‘西伯利亚’为试材,采用生物信息学方法,研究了百合SWEET基因家族成员编码蛋白的理化性质、二级结构、系统进化、保守结构域、Motif及基因表达模式,对百合SWEET基因家族成员进行鉴定,探究其在鳞茎发育过程中的作用,以期为该类基因生物学功能和百合地下茎生鳞茎发育机制的研究提供参考依据。结果表明:从转录组数据鉴定到了12个SWEET基因,所有家族成员都为稳定的疏水蛋白,且均含有MtN3_slv结构域,可划分为4个亚家族,不同成员在百合茎生鳞茎形成和进一步发育中差异表达。