儿童肺炎支原体肺炎肺泡灌洗液CARDS TX、HMGB1、TLR2表达及临床意义

目的研究肺炎支原体肺炎(MPP)患儿肺泡灌洗液中社区获得性呼吸窘迫综合征毒素(CARDS TX)、高迁移率族蛋白1(HMGB1)、Toll样受体2(TLR2)表达,探讨其在MPP发病中的临床意义。方法回顾性分析55例MPP病例组及20例对照组临床资料,同时检测两组支气管肺泡灌洗液(BALF)中CARDS TX、HMGB1、TLR2、TLR4、晚期糖基化终末产物受体(RAGE)及髓样分化因子88(MyD88)表达,体外检测不同浓度CARDS TX刺激下HMGB1、TLR2的表达并进行比较。结果与对照组相比,MPP组LYM绝对值计数、PA、CD3^(+)、CD3^(+)CD4^(+)、CD3^(+)CD8^(+)、CD3-CD19^(+)、NK cell、CD19^(+)CD23^(+)明显下降,CRP、LDH、D-二聚体、IgA、IgG、IgM、CARDS TX、HMGB1、TLR2、MyD88均升高,差异有统计学意义(P<0.05或0.001)。CARDS TX刺激后HMGB1、TLR2的表达升高,且呈正相关,差异有统计学意义(P<0.001)。结论CARDS TX可能通过HMGB1-TLR2-MyD88途径参与肺炎支原体(MP)的致病。

CARD6在脓毒症心肌损伤中的机制研究

目的:探讨CARD6在脓毒症心肌损伤中的调节机制。方法:将C57BL/6小鼠随机分为对照组与模型组。采用LPS腹腔注射法构建脓毒症心肌损伤小鼠模型,RT-qPCR检测小鼠心肌组织中CARD6、caspase-1的mRNA水平。体外实验则采用LPS刺激转染CARD6过表达质粒的H9C2细胞,检测caspase-1、细胞活力、CK-MB水平。结果:与对照组相比,模型组小鼠心肌组织中LDH、CK-MB含量,caspase-1表达水平上升,提示脓毒症导致小鼠心肌损伤,而CARD6表达水平明显降低。体外实验发现CARD6表达水平呈下降趋势,转染含有CARD6的过表达质粒后细胞释放CK-MB的水平降低、细胞活力增加、caspase-1水平降低。结论:CARD6可改善脓毒症所致心肌损伤,其机制可能与caspase-1介导的细胞焦亡相关。

肺炎支原体CARDS毒素依赖KELED序列调控炎性反应

目的细胞内逆向运输系统是微生物毒素蛋白导致细胞毒性的关键。KELED序列是肺炎支原体CARDS毒素逆向转运至内质网,而后发挥细胞毒性作用的关键氨基酸基序。在小鼠模型中,体外重组的CARDS蛋白可以重现肺炎支原体肺炎患者的特征性症状。然而,CARDS是否依赖于KELED序列调控肺组织炎性反应仍不清楚。方法在大肠杆菌中表达并纯化CARDS及其KELED序列突变蛋白;随后在细胞水平验证其致空泡化活性;最后,在小鼠模型中评价KELED序列突变的CARDS蛋白是否影响炎性因子的表达以及是否造成肺组织病理学损伤。结果KELED序列氨基酸发生突变,减弱CARDS蛋白致细胞空泡化效应,下调肺组织炎性因子mRNA表达,减轻肺组织的病理学损伤。结论KELED序列对CARDS蛋白致细胞空泡化及炎性损伤活性的维持至关重要。

Dynamic of phytoplankton community during varying intensities of the northeast monsoon in the Taiwan Strait

The characteristics of the terrain of a strait can lead to a“fine tube”effect that enhances a monsoon and thereby affects the physical,chemical,and biological processes of marine ecosystems.This effect is a highly dynamic and complex phenomenon involving interactions among atmospheric,oceanic,and terrestrial systems,as well as biogeochemical cycles and biological responses driven by it.However,current understanding has been focused mainly on the differences between monsoons,and there have been few studies concerned with the weakening or strengthening of monsoons.To explore the biogeochemical and phytoplankton responses during varying intensities of the northeast(NE)monsoon in the Taiwan Strait,high-resolution,across-front observations combined with FerryBox online data and satellite observations were conducted in this study during a strong,moderate,and weak NE monsoon.The spatiotemporal changes of nutrient concentrations and phytoplankton communities were regulated by the dynamics of ocean currents forced by NE winds.The weakening of the NE monsoon caused shrinkage of the coastal currents that led to a reduction of nutrient concentrations and an alteration of the distribution patterns of phytoplankton communities along cross-front sections.Specifically,there was a notable decrease in the proportions of dinoflagellates and cryptophytes in inshore regions and of prasinophytes in offshore areas.This study showed for the first time the dynamics of phytoplankton with changes of ocean currents during varying intensities of the NE monsoon in a strait system.The findings helped to elucidate the general spatial patterns of the phytoplankton community based on satellite-derived surface temperature and wind patterns and further enhanced the understanding of biogeochemical cycles in marine systems.

Credit Card Fraud Detection Using Improved Deep Learning Models

Fraud of credit cards is a major issue for financial organizations and individuals.As fraudulent actions become more complex,a demand for better fraud detection systems is rising.Deep learning approaches have shown promise in several fields,including detecting credit card fraud.However,the efficacy of these models is heavily dependent on the careful selection of appropriate hyperparameters.This paper introduces models that integrate deep learning models with hyperparameter tuning techniques to learn the patterns and relationships within credit card transaction data,thereby improving fraud detection.Three deep learning models:AutoEncoder(AE),Convolution Neural Network(CNN),and Long Short-Term Memory(LSTM)are proposed to investigate how hyperparameter adjustment impacts the efficacy of deep learning models used to identify credit card fraud.The experiments conducted on a European credit card fraud dataset using different hyperparameters and three deep learning models demonstrate that the proposed models achieve a tradeoff between detection rate and precision,leading these models to be effective in accurately predicting credit card fraud.The results demonstrate that LSTM significantly outperformed AE and CNN in terms of accuracy(99.2%),detection rate(93.3%),and area under the curve(96.3%).These proposed models have surpassed those of existing studies and are expected to make a significant contribution to the field of credit card fraud detection.

The response of surface sedimental diatoms to the environment and its potential significance in the Taiwan Strait,western Pacific

As the most important component of marine siliceous organisms,diatoms are vital primary producers of the ocean that are often used as indicators of paleoenvironmental change.To understand the response of sedimental diatoms to regional environmental changes and the factors affecting the distribution of sedimental diatoms in the Taiwan Strait,this study quantified and classified the diatoms found in surface sediments collected during four surveys from 2019 to 2020.Overall,118 diatom taxa and 44 genera were identified with total diatom abundance of 8-27353 valves/g.Four diatom assemblages representing different environments were identified.Among them,assemblageⅠrepresented a coastal environment,assemblageⅡcomprised warm water species of a coastal environment,AssemblageⅢrepresented a coastal environment affected markedly by exorheism,AssemblageⅣrepresented a group with lowest diatom abundance.Seasonal variation in total diatom abundance was controlled by seven environmental factors:depth,sea surface salinity,mean grain size,silicate,nitrite,nitrate,and phosphate.Spatiotemporal variation in each of the diatom assemblages was substantial and strongly affected by various currents,upwelling,and low-salinity water.Specifically,it was found that the succession of diatom assemblages reflects change in the range of influence of local warm currents.

A Self-Adapting and Efficient Dandelion Algorithm and Its Application to Feature Selection for Credit Card Fraud Detection

A dandelion algorithm(DA) is a recently developed intelligent optimization algorithm for function optimization problems. Many of its parameters need to be set by experience in DA,which might not be appropriate for all optimization problems. A self-adapting and efficient dandelion algorithm is proposed in this work to lower the number of DA's parameters and simplify DA's structure. Only the normal sowing operator is retained;while the other operators are discarded. An adaptive seeding radius strategy is designed for the core dandelion. The results show that the proposed algorithm achieves better performance on the standard test functions with less time consumption than its competitive peers. In addition, the proposed algorithm is applied to feature selection for credit card fraud detection(CCFD), and the results indicate that it can obtain higher classification and detection performance than the-state-of-the-art methods.

CARD11 serves as a therapeutic biomarker for the drug therapies of ccRCC

Background:The incidence of clear cell renal cell carcinoma(ccRCC)is globally high;however,despite the introduction of innovative drug therapies,there remains a lack of effective biomarkers for evaluating treatment response.Recently,Caspase recruiting domain-containing protein 11(CARD11)has garnered attention due to its significant association with tumor development and the immune system.Methods:The expression of CARD11 mRNA and protein in ccRCC were analyzed by public database and immunohistochemistry.The focus of this study is on the epigenomic modifications of CARD11,its expression of ccRCC immunophenotype,and its correlation with response to immunotherapy and targeted therapy.Furthermore,to investigate the mechanism of this molecule’s influence on different biological behaviors of cells,cell tests in vitro have been conducted to observe the impact of its expression level.Results:CARD11 expression was upregulated which may be mainly modified by body methylation and was correlated with poor prognosis in ccRCC.In the tumor microenvironment of ccRCC,CARD11 expression was positively correlated with increased T lymphocyte infiltration and increased expression of inhibitory immune checkpoints.Moreover,ccRCC patients with high CARD11 expression had a better response to immunotherapy and targeted therapy.The knockdown of CARD11 ultimately suppressed the proliferation,migration,and invasion capabilities of ccRCC cells while simultaneously enhancing tumor cell apoptosis.Conclusion:We identified CARD11 as a novel therapeutic biomarker for immunotherapy and targeted therapy in ccRCC.

Spatiotemporal variations of phytoplankton in the Taiwan Strait using lipid biomarkers and the potential influencing factors

The biological pump,driven by phytoplankton production and death,plays a crucial role in the ocean’s sequestration of atmospheric CO_(2).In particular,marginal seas with high primary productivity show a significant capacity for carbon fixation.Variations in phytoplankton biomass and community structure are key factors influencing the efficiency of the marine biological pump.The Taiwan Strait(TS)is a unique shallow conduit that connects the East China Sea(ECS)and the South China Sea(SCS),which are subject to seasonal monsoons and episodic events(e.g.,typhoons and floods).Thus,its planktonic ecosystem is significantly influenced by physical processes such as strong ocean currents,coastal upwelling and river discharge,resulting in noticeable seasonal variability.In this study,we examined spatiotemporal patterns of phytoplankton biomass and community structure using phytoplankton-sourced biomarkers from suspended particles in surface waters across all four seasons from 2019 to 2020 in the TS.The findings highlight notable seasonal disparities in phytoplankton biomass,with spring and summer exhibiting significantly higher levels compared to autumn and winter.In order to determine phytoplankton ecosystem responses to various physical and biological processes on a seasonal scale,we used Empirical Orthogonal/Eigen Function(EOF)analysis to investigate the covarying spatiotemporal patterns of:marine-sourced biomarkers and terrestrial-sourced biomarkers in surface suspended particles,a biomass indicator(Chl a),water-mass indicators[sea surface temperature(SST),sea surface salinity(SSS),nutrients],and a hydrodynamic indicator[total suspended solids at surface/bottom water,(TSS_S and TSS_B)].The results identified six physical-biological coupling modes that influence seasonal variations in marine phytoplankton ecosystems within the energetic strait system.Additionally,an in-depth understanding of the coupling between physical process and lipid biomarker signals from suspended particles in the contemporary marine environment can offer valuable insights for interpreting ancient sediment records of phytoplankton ecosystem evolution in the TS.

On the Ontology of Taiwan residents Films in the Post Declare Martial Law Ended Period

In addition to describing the social reality of Taiwan’s political and economic chaos, Taiwan’s films after the lifting of martial law also try to explore the ultimate significance of human existence, showing the color of ontology. Under the influence of postmodern culture, Taiwan films adopt non-linear narrative methods such as fragmented, time countercurrent or psychological time, emphasizing people’s instant experience and expressing modern people’s anxiety and social absurdity. In addition, the film highlights the gridding and deformed space, so as to highlight the interpersonal alienation and survival dilemma of modern cities.Taiwan residents films also reflect the impermanence of fate, the nothingness of existence, and the absurdity of society through the death of characters. It is also coupled with the existential view of “living to death” and “others are hell”.

Potential Impact of COP28 Resolution on Taiwan

After two weeks of discussions and consultations at the 28 th Conference of the Parties to the United Nations Framework Convention on Climate Change(COP28),states parties finally reached a series of important sustainable development resolutions and voluntary initiatives.In this paper,the possible impact of these resolutions on Taiwan was mainly discussed,and the relevant regulations and policies that the Taiwan authorities may enact were analyzed.

Logistics,Supply Chain Management,and Taiwan residents Immigration Assessed in Light of Globalization

Organizations regardless of size can take advantage of the market dynamics brought about by globalization.Supply chain management is crucial for large organizations,especially with regard to the costs,complexity,and risks of its large,often global,supply chains.From technology to risk management,learning what it takes to navigate transportation and logistics business management in today’s volatile world is critical.Human migration has accelerated in light of globalization and rapid advances in transportation(particularly aviation)technology.This study investigated the connection between logistics and supply chain management in the age of globalization;reviewed the migration,settlement,and naturalization of Taiwan residents immigrants along the West Coast of the United States;and theoretically analyzed the forces that have led to the construction of the Taiwan residents immigrant community.Taiwan residents immigrants and their settlement are affected by global forces,and factors such as their cross-cultural trade,the characteristics of diasporic communities,and their national identity can provide scholarly and pedagogical insights.

A Study on the Problem of Employee Punch Card Substitution in Enterprise Management:The Case of Jingdong Enterprise

With economic progress and the continuous advancement of science and technology,the issue of employees substituting punch cards has gradually become a significant challenge in enterprise management.The purpose of this paper is to discuss the causes,effects,and countermeasures of the employee punch card phenomenon,with the aim of providing effective management recommendations for Chinese enterprises.In practice,enterprises should flexibly apply the countermeasures proposed in this paper according to their specific circumstances to prevent substitute punch card incidents and improve overall management efficiency.

肺炎支原体CARDS毒素蛋白多表位拼接抗原的表达及鉴定

目的探讨肺炎支原体CARDS毒素蛋白多表位拼接抗原的免疫反应活性。方法对CARDS毒素蛋白的抗原表位进行分析,选取10个重要的表位进行拼接,反向翻译后合成并克隆,插入pET28a表达载体构建pET-CARDS表达质粒并转入受体菌。经IPTG诱导表达的多表位拼接CARDS蛋白使用6×His单克隆抗体和人阳性血清进行了免疫印迹的检测。结果 CARDS毒素蛋白的多表位拼接抗原表达载体构建成功,诱导后表达大小为30KDa的重组蛋白,Western blot测定其能与6×His单克隆抗体和人阳性血清发生反应。结论本研究选取的CARDS毒素蛋白抗原表位具有较强的免疫活性,可为Mp感染的诊断提供新的候选抗原。

Cloud Card对个人学习空间建设的新启示

近年来,个人学习空间建设走向实践研究,旨在为学习者提供智慧化学习环境。但由于数字资源组织仍然杂乱无序、适应性和联通性较差,学习工具难以满足学习者个性化需求,基于功能模块的数据服务难以支持真实性评价等原因,个人学习空间取得的阶段性成效与预期尚存在较大距离。该文借鉴深层链接技术在Google、Twitter、Facebook等商业广告领域的成功经验,尝试将其新的发展延伸——Cloud Card引入到个人学习空间建设中。在对Cloud Card进行技术追溯和需求分析的基础上,详细阐述了Cloud Card的UI关联展现、流线型服务组织模式、底层隐形数据传递等特点,并从工具服务的情境化组织、学习过程的追踪、面向过程的真实性评价三个方面,重点探讨了Cloud Card在教育应用中的可行性和关键设计要素,以期能为"人人通"的实现与发展提供一种新的理念和技术路线,为个人学习空间建设助力。

工字钢CARD数模精度与系统功能的改善

分析了工字钢腿部各个部分的变形系数模型,并对腿高长缩模型进行了模拟实验,在此基础上讨论了完善工字钢CARD程序系统功能的几种可能的途径。

中国台湾光复后实行行政长官公署制的核心原因、利弊得失与启迪

1945年,国民政府突然将中国台湾光复后实行的行政体制从“省制”改为“特殊省制”。一般认为此是陈仪主导的中国台湾调查委员会设计所致,但随着史料的解密,发现中国台湾调查委员会的设计意见及方案是“果”,导致该转变的核心原因是美国军事占领中国台湾计划的改变。作为“特殊省制”,中国台湾省行政长官公署的组织架构和行政长官权力类似于日本殖民时期的“台湾总督府”,带给中国台湾人民强烈的殖民遗绪之感,为“二二八”事件的爆发埋下隐患。光复初期,中国台湾实行“特殊省制”固然有其必然性和必要性,但中国台湾有别于祖国内地其他省区的特殊性更决定了其具有临时性和时效性。

NOD2/CARD15基因突变与中国人克罗恩病相关性的研究

背景:近年多项研究证明NOD2/CARD15基因序列的单核苷酸多态性(SNP)与西方白种人克罗恩病(CD)明显相关,其中3个SNP(R702W、G908R和3020insC)与CD的相关性尤为显著。目的:探讨NOD2/CARD15基因SNP与中国人CD发病的相关性及其与CD临床特点的关系。方法:选取临床资料完整的CD患者48例、溃疡性结肠炎(UC)患者和健康对照者各50例,提取人血白细胞基因组DNA,经聚合酶链反应(PCR)扩增NOD2基因全部12对外显子,纯化后直接测序,根据结果分析其突变与CD病变特点的关系。结果:CD组、UC组和健康对照组均未检出3个西方人常见的NOD2/CARD15基因多态性位点。CD组的P268S突变率显著高于UC组和健康对照组(P<0.05)。5例P268S突变的CD患者病变均位于回肠(P<0.01),4例发病年龄≤20岁(P<0.01),且均并发肠腔狭窄(P<0.01)。结论:中国人CD患者中存在NOD2/CARD15基因P268S突变,且与患者的发病年龄、病变部位和并发症相关,有必要对其功能作进一步探讨。

P268S突变型NOD2/CARD15真核表达载体的构建及其体外表达

背景:NOD2/CARD15基因序列单核苷酸多态性(SNP)与欧美人群的克罗恩病(CD)明显相关,其中R702W、G908R和3020insC3个SNP位点与CD的相关性尤为显著。而日本、韩国以及我国香港和浙江地区的研究均未发现上述3个SNP的改变,但最近研究发现了可能与中国人CD相关的P268S突变。目的:构建P268S突变型NOD2/CARD15真核表达载体和体外转染体系,为研究突变型NOD2/CARD15的功能提供实验基础。方法:应用定点诱变技术构建P268S突变型NOD2/CARD15真核表达载体,以阳离子脂质体介导体外转染技术瞬时转染人胚肾细胞HEK293T,以蛋白质印迹法和逆转录聚合酶链反应(RT-PCR)检测HEK293T细胞NOD2/CARD15的表达。结果:经克隆、酶切、测序证实获得P268S突变型NOD2/CARD15基因,突变载体转入HEK293T细胞后,NOD2/CARD15有效表达。结论:成功构建了P268S突变型NOD2/CARD15真核表达载体,阳离子脂质体是人胚肾细胞有效的体外转染体系。

基于Express Card接口的千兆以太网网卡设计

针对现最常用的10/100Mbps自适应网卡网速过慢的问题,设计了一种含Express Card接口的千兆以太网网卡;用户可以方便、快捷地将其插入笔记本电脑的Express Card接口,实现高速通信;与以往采用FPGA、DSP等芯片设计的千兆以太网网卡相比,该千兆以太网网卡采用内含以太网MAC控制器、10Mbps/100Mbps/1000Mbps自适应收发器、PCI Express总线控制器等的网卡芯片RTL8111DL作为主芯片,无需再外接一片物理层芯片和一片MAC芯片;经测试:笔记本电脑可以通过此千兆以太网网卡借助五类线与外部设备握手成功,实现约260兆的高速数据传输。