“Ⅰ+Ⅱ”疗法对高血压肥胖大鼠血清肿瘤坏死因子α含量的影响

[目的]观察“Ⅰ+Ⅱ”疗法对高血压肥胖大鼠肿瘤坏死因子α(TNF-α)水平的影响及意义。[方法]以营养饲料饲养自发性高血压大鼠(SHR)造模成高血压肥胖大鼠,观察“Ⅰ+Ⅱ”疗法治疗前后高血压肥胖大鼠血压、体重、身长和血清TNF-α水平,并与基础治疗、中药治疗和针灸治疗进行比较。[结果]高血压肥胖大鼠血压、体重、lee’s指数和TNF-α水平明显升高。经“Ⅰ+Ⅱ”疗法治疗30天后,高血压肥胖大鼠血压、体重、lee’s指数和TNF-α水平有所下降。[结论]“Ⅰ+Ⅱ”疗法具有减肥降压作用,其机理可能与降低循环TNF-α水平有关。

“Ⅰ+Ⅱ”疗法对高血压肥胖大鼠血清NO、血浆ET的影响

目的探讨“Ⅰ+Ⅱ”疗法对高血压肥胖大鼠模型体重、体重指数、血压及血清NO、血浆ET的影响。方法54只SHR造模后,将模型大鼠分为空白组、模型组、基础治疗组、中药治疗组、针灸治疗组、综合治疗组,观察30天后,测血清NO、血浆ET水平。结果各治疗组体重、体重指数、血压及血浆ET较治疗前均有明显改变,血清NO较治疗前变化不明显。结论“Ⅰ+Ⅱ”疗法能明显降低高血压肥胖大鼠模型的体重、体重指数和血压;抑制血管内皮细胞分泌ET,阻止血清NO水平进一步下降,平衡血管舒缩因子比例是其降压作用的重要机制之一。

“Ⅰ+Ⅱ”疗法对原发性高血压肥胖大鼠减肥降压的作用研究

目的:从动物实验方面论证“Ⅰ+Ⅱ”疗法的疗效,并初步探讨其作用机制。方法:原发性高血压大鼠(SHR)54只,随即分为两组:普食组(6只)和营养饲料组(48只)。90天后,将所获高血压肥胖模型大鼠随机分入模型组(5只)、空白组(5只)、基础组(5只)、中药组(5只)、针灸组(5只)和综合组(6只),采用相应的方法干预治疗,并定期观察期间血压、体重及身长的变化。治疗结束后,取腹膜后脂肪垫称重;取用部分腹主动脉用于病理切片;测定空腹血脂(TC、TG、HDL-C、LDL-C)、血糖、血清胰岛素和瘦素,并计算Lee’s指数、脂肪系数和胰岛素敏感指数(ISI)。结果:高血压肥胖模型大鼠存在胰岛素和瘦素抵抗。通过不同疗法治疗后,高血压肥胖模型大鼠的血压、Lee’s指数、脂肪系数、血脂、胰岛素敏感指数(ISI)、瘦素水平以及腹主动脉病变等均得到了不同程度的改善。结论:“Ⅰ+Ⅱ”疗法对于原发性高血压肥胖大鼠取得了良好的减肥降压效果,胰岛素和瘦素抵抗可能是其重要作用途径。

“Ⅰ+Ⅱ”疗法对高血压肥胖大鼠血浆神经肽Y含量的影响

目的:观察“Ⅰ+Ⅱ”疗法对高血压肥胖大鼠神经肽Y(NPY)水平的影响。方法:以营养饲料饲养自发性高血压大鼠(SHR)照模成高血压肥胖大鼠,观察“Ⅰ+Ⅱ”疗法治疗前后高血压肥胖大鼠血压、体重、身长和血浆NPY水平,并与基础治疗、中药治疗和针灸治疗进行比较。结果:经“Ⅰ+Ⅱ”疗法治疗30天后,高血压肥胖大鼠血压、体重、lee's指数和NPY水平有所下降。结论:“Ⅰ+Ⅱ”疗法具有减肥降压作用。

Effect of Acupuncture-moxibustion on TNF-α,sTNFR-Ⅰ and sTNFR-Ⅱ of Rats with Crohn's Disease

Objective： To observe the effect of acupuncture-moxibustion on TNF-α, sTNFR-Ⅰ and sTNFR-Ⅱ in rats with Crohn＇s disease （CD）. Method： The models of CD rats induced by TNBS were randomized into model group, herbal cake-partitioned moxibustion group and electroacupuncture group as well as a normal control group. The histopathological changes of colon mucus membrane were observed with HE staining and the contents of TNF-α, sTNFR-Ⅰ and sTNFR-Ⅱ were detected with ELISA method. Results： When compared with the normal group, the TNF-α level in CD rats was substantially elevated and the sTNFR-Ⅰ and sTNFR-Ⅱ showed no marked changes. After herbal cake-partitioned moxibustion and electroacupuncture treatment, the TNF-α level in CD rats was substantially reduced and sTNFR-Ⅰ and sTNFR-Ⅱ showed no marked changes. The inflammatory lesions and abnormal structures of CD rats were significantly improved after herbal cake-partitioned moxibustion and electric stimulation. Conclusion： Herbal cake-partitioned moxibustion and electroacupuncture can both remarkably reduce the TNF-α level in blood serum, and this might be one of the action mechanisms in the treatment of CD.