The early Miocene in the Zhu Ⅲ subbasin, the Pearl River Mouth basin, includes two formations-Zhujiang and Zhuhai. There are 8 third-order sequences, S1, S2, S3, S4, S5, S6, S7 and S8 from the bottom of Zhuhai to th...The early Miocene in the Zhu Ⅲ subbasin, the Pearl River Mouth basin, includes two formations-Zhujiang and Zhuhai. There are 8 third-order sequences, S1, S2, S3, S4, S5, S6, S7 and S8 from the bottom of Zhuhai to the top of Zhujiang in thee two formations. There are only one transgressive systems tract (TST) and one highstand systems tract (HST) in each sequence because the whole Zhu Ⅲ subbasin was located updip the shelf break during sequence deposition. The boundaries and maximum flooding surfaces (mfs) are in good response to both gamma and acoustic log curves in the study area. In seismic profile 1249, sediments obviously onlap over the unconformity (SB0, the bottom of Zhuhai Fm), SB1 and SB2, but obviously over only SB2 in seismic profile 1283 since the well- devel-oped faults in the subbasin. The sand bodies with high porosity and permeability for Petroleum migration and accumulation had been reworked by tidal currents before their burial. Hence, the tidal influenced parasequence sets occur both in TST and HST. Through detailed analysis, the sand bodies in TST are more favorable for Petroleum to migrate and accumulate than those in HST.展开更多
为了探讨多聚AFPⅢ的作用机制,从南极鱼Lycodichthys dearborni的多聚三型抗冻蛋白基因LD12 c DNA中克隆得到AFPⅢ的四聚体,命名为LD4,并构建真核表达质粒Tol2-actin-LD4-2A-EGFP。将表达质粒转染到斑马鱼细胞系ZF4中,发现LD4可以在ZF4...为了探讨多聚AFPⅢ的作用机制,从南极鱼Lycodichthys dearborni的多聚三型抗冻蛋白基因LD12 c DNA中克隆得到AFPⅢ的四聚体,命名为LD4,并构建真核表达质粒Tol2-actin-LD4-2A-EGFP。将表达质粒转染到斑马鱼细胞系ZF4中,发现LD4可以在ZF4中大量表达并且能够减少斑马鱼细胞在低温胁迫下的死亡率。通过对不同处理温度(28、18、10℃)下的WT、EGFP和LD4细胞进行转录组测序分析,找出26个表达差异转录因子,其中I3MB13、ZNF687b等表达上调,JUN、Cremb等表达下调,并且通过荧光定量PCR的验证。通过KEGG pathway分析发现,这些差异性基因主要参与细胞凋亡、细胞周期、增殖等调节通路。采用Annexin V-PE/7-AAD双染色法对3种不同温度下的WT、EGFP和LD4细胞进行细胞凋亡检测,结果显示,LD4在低温下与对照组相比凋亡率没有显著差异,说明LD4可能是通过其他通路而不是通过抑制细胞凋亡来抵御低温胁迫,这为LD4作用机制的进一步研究提供了理论基础。展开更多
To characterize the Fe(III)-reducing bacteria,enrichment cultures were initiated by inoculating deep-sea sediment from the South China Sea(SCS)into the media with hydrous ferric oxide(HFO)as the sole electron acceptor...To characterize the Fe(III)-reducing bacteria,enrichment cultures were initiated by inoculating deep-sea sediment from the South China Sea(SCS)into the media with hydrous ferric oxide(HFO)as the sole electron acceptor.As indicated by Meta 16S rDNA Amplicon Sequencing,the microorganisms related to Fe(III)-reduction in the enrichment cultures were mainly Shewanella and Enterobacter.A new facultative Fe(III)-reducing bacterium was obtained and identified as Enterobacter sp.Nan-1 based on its 16S rRNA gene sequence and physiological characterizations.Enterobacter sp.Nan-1 was not only a mesophilic bacterium capable of reducing HFO with a wide range of salinity(4,34,40,50 and 60 g L−1)efficiently,but also a piezotolerant bacterium that can proceed Fe(III)-reduction sustainedly at hydrostatic pressures between 0.1 and 50 MPa using glucose and pyruvate as carbon source.Furthermore,the geochemical characteristics of deep-sea sediment indicated that the microbial metabolism and iron reduction both remain active in the well-developed Fe(III)-reducing zone where the strain Nan-1 was obtained.To our knowledge,Enterobacter sp.Nan-1 could serve as a new applicative Fe(III)-reducing bacterium for future investigation on the iron biogeochemical cycle and diagenetic process of organic matter in the deep-sea environment.展开更多
Scorpion venoms contain several kinds of neurotoxins, such as antimammalian neurotoxins, anti-insect neurotoxins and others. But most of them form a family of structurally related single chain proteins of 60—70 amino...Scorpion venoms contain several kinds of neurotoxins, such as antimammalian neurotoxins, anti-insect neurotoxins and others. But most of them form a family of structurally related single chain proteins of 60—70 amino acid residues and selectively interact with voltage-dependent sodium channels in different excitable cells, only a few minipeptides of 31—39 amino acid residues are proved to block potassium channels. As a kind of molecular probe, scorpion neurotoxins have been widely used for analyzing the展开更多
基金This study is financially supported by the National Natural Science Foundation of China (No. 49732005-01).
文摘The early Miocene in the Zhu Ⅲ subbasin, the Pearl River Mouth basin, includes two formations-Zhujiang and Zhuhai. There are 8 third-order sequences, S1, S2, S3, S4, S5, S6, S7 and S8 from the bottom of Zhuhai to the top of Zhujiang in thee two formations. There are only one transgressive systems tract (TST) and one highstand systems tract (HST) in each sequence because the whole Zhu Ⅲ subbasin was located updip the shelf break during sequence deposition. The boundaries and maximum flooding surfaces (mfs) are in good response to both gamma and acoustic log curves in the study area. In seismic profile 1249, sediments obviously onlap over the unconformity (SB0, the bottom of Zhuhai Fm), SB1 and SB2, but obviously over only SB2 in seismic profile 1283 since the well- devel-oped faults in the subbasin. The sand bodies with high porosity and permeability for Petroleum migration and accumulation had been reworked by tidal currents before their burial. Hence, the tidal influenced parasequence sets occur both in TST and HST. Through detailed analysis, the sand bodies in TST are more favorable for Petroleum to migrate and accumulate than those in HST.
基金the financial support by the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB06020000)the Zhejiang Geological Prospecting Bureau Science Projects(No.201713)the Geological Fund of Zhejiang Province(No.20150012).
文摘To characterize the Fe(III)-reducing bacteria,enrichment cultures were initiated by inoculating deep-sea sediment from the South China Sea(SCS)into the media with hydrous ferric oxide(HFO)as the sole electron acceptor.As indicated by Meta 16S rDNA Amplicon Sequencing,the microorganisms related to Fe(III)-reduction in the enrichment cultures were mainly Shewanella and Enterobacter.A new facultative Fe(III)-reducing bacterium was obtained and identified as Enterobacter sp.Nan-1 based on its 16S rRNA gene sequence and physiological characterizations.Enterobacter sp.Nan-1 was not only a mesophilic bacterium capable of reducing HFO with a wide range of salinity(4,34,40,50 and 60 g L−1)efficiently,but also a piezotolerant bacterium that can proceed Fe(III)-reduction sustainedly at hydrostatic pressures between 0.1 and 50 MPa using glucose and pyruvate as carbon source.Furthermore,the geochemical characteristics of deep-sea sediment indicated that the microbial metabolism and iron reduction both remain active in the well-developed Fe(III)-reducing zone where the strain Nan-1 was obtained.To our knowledge,Enterobacter sp.Nan-1 could serve as a new applicative Fe(III)-reducing bacterium for future investigation on the iron biogeochemical cycle and diagenetic process of organic matter in the deep-sea environment.
基金This Work was respectively supported in part by a grant for Youth Foundation (388008) the National Natural Science Foundation of China and the Grant-in-Aid for Overeas Scientific Reseatrch from the Ministry of Education, Science Culture, Japan
文摘Scorpion venoms contain several kinds of neurotoxins, such as antimammalian neurotoxins, anti-insect neurotoxins and others. But most of them form a family of structurally related single chain proteins of 60—70 amino acid residues and selectively interact with voltage-dependent sodium channels in different excitable cells, only a few minipeptides of 31—39 amino acid residues are proved to block potassium channels. As a kind of molecular probe, scorpion neurotoxins have been widely used for analyzing the
