目的探讨儿童过敏性鼻炎(allergic rhinitis,AR)和支气管哮喘(bronchial asthma,BA)患儿外周血高迁移率族蛋白B1(high mobility group box 1,HMGB1)水平与辅助性T细胞17(T helper 17 cell,Th17)和调节性T细胞(regulatory T cell,Treg)...目的探讨儿童过敏性鼻炎(allergic rhinitis,AR)和支气管哮喘(bronchial asthma,BA)患儿外周血高迁移率族蛋白B1(high mobility group box 1,HMGB1)水平与辅助性T细胞17(T helper 17 cell,Th17)和调节性T细胞(regulatory T cell,Treg)的比例(Th17/Treg)的相关性。方法采用前瞻性研究方法,选取2023年3月至12月在解放军总医院第七医学中心门急诊就诊患儿及健康体检者共113例作为研究对象,按照临床症状分成健康对照组(n=25)、过敏性鼻炎组(AR组,n=28)、支气管哮喘组(BA组,n=31)和两者并有组(AR+BA组,n=29),比较4组肺功能、免疫球蛋白E(immunoglobulin E,IgE)、白细胞介素(interleukin,IL)-17、HMGB1水平和Th17/Treg比例。统计学方法采用t检验、方差分析和Pearson相关性分析。结果各组的第1秒用力呼气容积(forced expiratory volume in the first second,FEV1)的结果显示,健康对照组高于AR组、BA组和AR+BA组[(96.4±2.5)%、(90.7±4.9)%、(88.6±4.1)%、(82.1±5.5)%,F=47.193,P值均<0.001],显示AR和BA患儿肺功能可能变差。AR组、BA组和AR+BA组HMGB1水平高于健康对照组[(30.4±12.4)mg/L、(47.2±17.8)mg/L、(51.4±21.8)mg/L、(4.3±2.1)mg/L,F=48.896,P值均<0.001]。AR组、BA组和AR+BA组Th17/Treg比例高于健康对照组(0.68±0.14、0.71±0.14、1.36±0.40、0.30±0.07,F=105.547,P值均<0.001)。Pearson相关性分析结果显示,HMGB1与Th17(r=0.521,P<0.001)、Th17/Treg(r=0.419,P<0.05)、IgE(r=0.502,P<0.05)、IL-17(r=0.472,P<0.05)呈正相关,与FEV1(r=-0.645,P<0.001)呈负相关。结论在儿童AR合并BA的发病过程中,HMGB1水平及Th17细胞百分比增加,而Treg细胞百分比降低,Th17/Treg比例存在失衡的情形,HMGB1水平与Th17细胞百分比、Th17/Treg比例、IgE、IL-17及FEV1存在一定的相关性,HMGB1和Th17/Treg的动态平衡可能通过影响细胞因子的表达而起到一定的调节作用。展开更多
Objective:To explore the balance of peripheral blood T helper 17 cells/regulatory T cell(Th17/Treg)ratio and the polarization ratio of M1 and M2 macrophages in lower extremity arteriosclerosis obliterans(ASO).Methods:...Objective:To explore the balance of peripheral blood T helper 17 cells/regulatory T cell(Th17/Treg)ratio and the polarization ratio of M1 and M2 macrophages in lower extremity arteriosclerosis obliterans(ASO).Methods:A rat model of lower extremity ASO was established,and blood samples from patients with lower extremity ASO before and after surgery were obtained.ELISA was used to detect interleukin 6(IL-6),IL-10,and IL-17.Real-time RCR and Western blot analyses were used to detect Foxp3,IL-6,IL-10,and IL-17 expression.Moreover,flow cytometry was applied to detect the Th17/Treg ratio and M1/M2 ratio.Results:Compared with the control group,the iliac artery wall of ASO rats showed significant hyperplasia,and the concentrations of cholesterol and triglyceride were significantly increased(P<0.01),indicating the successful establishment of ASO.Moreover,the levels of IL-6 and IL-17 in ASO rats were pronouncedly increased(P<0.05),while the IL-10 level was significantly decreased(P<0.05).In addition to increased IL-6 and IL-17 levels,the mRNA and protein levels of Foxp3 and IL-10 in ASO rats were significantly decreased compared with the control group.The Th17/Treg and M1/M2 ratios in the ASO group were markedly increased(P<0.05).These alternations were also observed in ASO patients.After endovascular surgery(such as percutaneous transluminal angioplasty and arterial stenting),all these changes were significantly improved(P<0.05).Conclusions:The Th17/Treg and M1/M2 ratios were significantly increased in ASO,and surgery can effectively improve the balance of Th17/Treg,and reduce the ratio of M1/M2,and the expression of inflammatory factors.展开更多
文摘目的探讨儿童过敏性鼻炎(allergic rhinitis,AR)和支气管哮喘(bronchial asthma,BA)患儿外周血高迁移率族蛋白B1(high mobility group box 1,HMGB1)水平与辅助性T细胞17(T helper 17 cell,Th17)和调节性T细胞(regulatory T cell,Treg)的比例(Th17/Treg)的相关性。方法采用前瞻性研究方法,选取2023年3月至12月在解放军总医院第七医学中心门急诊就诊患儿及健康体检者共113例作为研究对象,按照临床症状分成健康对照组(n=25)、过敏性鼻炎组(AR组,n=28)、支气管哮喘组(BA组,n=31)和两者并有组(AR+BA组,n=29),比较4组肺功能、免疫球蛋白E(immunoglobulin E,IgE)、白细胞介素(interleukin,IL)-17、HMGB1水平和Th17/Treg比例。统计学方法采用t检验、方差分析和Pearson相关性分析。结果各组的第1秒用力呼气容积(forced expiratory volume in the first second,FEV1)的结果显示,健康对照组高于AR组、BA组和AR+BA组[(96.4±2.5)%、(90.7±4.9)%、(88.6±4.1)%、(82.1±5.5)%,F=47.193,P值均<0.001],显示AR和BA患儿肺功能可能变差。AR组、BA组和AR+BA组HMGB1水平高于健康对照组[(30.4±12.4)mg/L、(47.2±17.8)mg/L、(51.4±21.8)mg/L、(4.3±2.1)mg/L,F=48.896,P值均<0.001]。AR组、BA组和AR+BA组Th17/Treg比例高于健康对照组(0.68±0.14、0.71±0.14、1.36±0.40、0.30±0.07,F=105.547,P值均<0.001)。Pearson相关性分析结果显示,HMGB1与Th17(r=0.521,P<0.001)、Th17/Treg(r=0.419,P<0.05)、IgE(r=0.502,P<0.05)、IL-17(r=0.472,P<0.05)呈正相关,与FEV1(r=-0.645,P<0.001)呈负相关。结论在儿童AR合并BA的发病过程中,HMGB1水平及Th17细胞百分比增加,而Treg细胞百分比降低,Th17/Treg比例存在失衡的情形,HMGB1水平与Th17细胞百分比、Th17/Treg比例、IgE、IL-17及FEV1存在一定的相关性,HMGB1和Th17/Treg的动态平衡可能通过影响细胞因子的表达而起到一定的调节作用。
基金supported by Natural Science Foundation of Hainan Province(820MS135)Hainan Provincial Health Commission 2023 Provincial Key Clinical Discipline(Clinical Medical Center)Construction Unit Fund Project(Qiongwei Yihan[2022]No.341)Hainan Provincial Health Technology Innovation Joint Project(WSJK2024MS209).
文摘Objective:To explore the balance of peripheral blood T helper 17 cells/regulatory T cell(Th17/Treg)ratio and the polarization ratio of M1 and M2 macrophages in lower extremity arteriosclerosis obliterans(ASO).Methods:A rat model of lower extremity ASO was established,and blood samples from patients with lower extremity ASO before and after surgery were obtained.ELISA was used to detect interleukin 6(IL-6),IL-10,and IL-17.Real-time RCR and Western blot analyses were used to detect Foxp3,IL-6,IL-10,and IL-17 expression.Moreover,flow cytometry was applied to detect the Th17/Treg ratio and M1/M2 ratio.Results:Compared with the control group,the iliac artery wall of ASO rats showed significant hyperplasia,and the concentrations of cholesterol and triglyceride were significantly increased(P<0.01),indicating the successful establishment of ASO.Moreover,the levels of IL-6 and IL-17 in ASO rats were pronouncedly increased(P<0.05),while the IL-10 level was significantly decreased(P<0.05).In addition to increased IL-6 and IL-17 levels,the mRNA and protein levels of Foxp3 and IL-10 in ASO rats were significantly decreased compared with the control group.The Th17/Treg and M1/M2 ratios in the ASO group were markedly increased(P<0.05).These alternations were also observed in ASO patients.After endovascular surgery(such as percutaneous transluminal angioplasty and arterial stenting),all these changes were significantly improved(P<0.05).Conclusions:The Th17/Treg and M1/M2 ratios were significantly increased in ASO,and surgery can effectively improve the balance of Th17/Treg,and reduce the ratio of M1/M2,and the expression of inflammatory factors.
