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Lecturers’Efforts in Building Rapport in the English-Medium Instruction(EMI)Context:Focus on the Use of Communication Strategies
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作者 Shiyan YU Jagdish KAUR 《Chinese Journal of Applied Linguistics》 2024年第3期498-513,526,共17页
Past studies reveal the prevalence of anxiety,coupled with low motivation and disengagement among students in English-medium instruction(EMI)programs.Given the detrimental impact these negative emotions can have on le... Past studies reveal the prevalence of anxiety,coupled with low motivation and disengagement among students in English-medium instruction(EMI)programs.Given the detrimental impact these negative emotions can have on learning outcomes,it is imperative that teachers establish positive emotional rapport with their students.This study explores how experienced and highly rated EMI lecturers at a Chinese university’s overseas campus use communication strategies to build rapport with their students during interactive academic activities.It identifies the strategies used by these lecturers and examines how the strategies facilitate the teaching-learning process.The data,consisting of 10 hours of tutorials and 10 hours of supervisor-student supervision meetings,is analyzed using an adapted Conversation Analysis(CA)approach.The analysis reveals three types of communication strategies(CSs)frequently used by lecturers:back-channeling,codeswitching,and co-creation of messages.By employing these strategies,the lecturers established a strong rapport with the students,which created an encouraging and supportive learning environment.Consequently,this positive atmosphere facilitated students’learning of content knowledge through English.The findings of this study have implications for the training of lecturers who encounter difficulties in establishing rapport with multilingual students in the EMI setting. 展开更多
关键词 communication strategies English-medium instruction(EMI) rapport-building lecturer-student interaction supportive learning environment
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“原因前置词+X+的+关系”结构及其形成
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作者 焦浩 《汉语学习》 北大核心 2023年第1期41-49,共9页
“原因前置词+X+的+关系”结构中“关系”表原因,具有后置词性质。该结构有六种形式,文章重点描写其句法功能与分布,分析X的语义特点,对“关系”表原因的后置词用法进行了溯源。“关系”本指某种具体关系,与X所指相同,这种同一性是结构... “原因前置词+X+的+关系”结构中“关系”表原因,具有后置词性质。该结构有六种形式,文章重点描写其句法功能与分布,分析X的语义特点,对“关系”表原因的后置词用法进行了溯源。“关系”本指某种具体关系,与X所指相同,这种同一性是结构发生重新分析和“关系”语法化的诱因。X从该结构形成初期就显示出以消极性语义为主的倾向,X为NP时经常隐含消极语义。 展开更多
关键词 关系 后置词 原因 消极性语义
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Determination of local constitutive behavior and simulation on tensile test of 2219-T87 aluminum alloy GTAW joints 被引量:6
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作者 李艳军 李权 +5 位作者 吴爱萍 麻宁绪 王国庆 Hidekazu MURAKAWA 鄢东洋 吴会强 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2015年第9期3072-3079,共8页
The local and global mechanical responses of gas tungsten arc welds(GTAW) of a 2219-T87 aluminum alloy were investigated with experiment and numerical simulation.Digital image correlation(DIC) was used to access t... The local and global mechanical responses of gas tungsten arc welds(GTAW) of a 2219-T87 aluminum alloy were investigated with experiment and numerical simulation.Digital image correlation(DIC) was used to access the local strain fields in transversely loaded welds and to determine the local stress-strain curves of various regions in the joint.The results show that the DIC method is efficient to acquire the local stress-strain curves but the curves of harder regions are incomplete because the stress and strain ranges are limited by the weakest region.With appropriate extrapolation,the complete local stress-strain curves were acquired and proved to be effective to predict the tensile behavior of the welded joint.During the tensile process,the fracture initiates from the weld toes owing to their plastic strain concentrations and then propagates along the fusion line,finally propagates into the partially melted zone(PMZ). 展开更多
关键词 aluminum alloy tensile behavior digital image correlation constitutive behavior welded joint
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Vegetation-Climate Relationship and Its Application in the Division of Vegetation Zone in China 被引量:38
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作者 方精云 宋永昌 +1 位作者 刘鸿雁 朴世龙 《Acta Botanica Sinica》 CSCD 2002年第9期1105-1122,共18页
Distribution of vegetation is closely coupled with climate; the climate controls distribution of vegetation and the vegetation type reflects regional climates. To reveal vegetation_climate relationships is the foundat... Distribution of vegetation is closely coupled with climate; the climate controls distribution of vegetation and the vegetation type reflects regional climates. To reveal vegetation_climate relationships is the foundation for understanding the vegetation distribution and theoretically serving vegetation regionalization. Vegetation regionalization is a theoretical integration of vegetation studies and provides a base for physiogeographical regionalization as well as agriculture and forestry regionalization. Based on a brief historical overview on studies of vegetation_climate relationships and vegetation regionalization conducted in China, we review the principles, bases and major schemes of previous vegetation regionalization and discuss on several contentious boundaries of vegetation zones in the present paper. We proposed that, under the circumstances that the primary vegetation has been destroyed in most parts of China, the division of vegetation zones/regions should be based on the distribution of primary and its secondary vegetation types and climatic indices that delimit distribution of the vegetation types. This not only reveals the closed relationship between vegetation and climate, but also is feasible practically. Although there still are divergence of views on the name and their boundaries of the several vegetation zones, it is commonly accepted that there are eight major vegetation regions in China, i.e. cold temperate needleleaf forest region, temperate needleleaf and broadleaf mixed forest region, warm temperate deciduous broadleaf forest region, subtropical evergreen broadleaf forest region, tropical monsoon forest and rain forest region, temperate steppe region, temperate desert region, and Qinghai_Xizang (Tibetan) Plateau high_cold vegetation region. Analyzing characteristics of vegetation and climate of major vegetation boundaries, we suggested that: 1) Qinling Mountain_Huaihe River line is an important arid/humid climatic, but not a thermal climatic boundary, and thus can not also be regarded as the northern limit of the subtropical vegetation zone; 2) the northern limit of subtropical vegetation zone in China is along the northern coast of the Yangtze River, from Hangzhou Bay, via Taihu Lake, Xuancheng and Tongling in Anhui Province, through by southern slope of the Dabie Mountains, to Wuhan and its west, coinciding with a warmth index ( WI ) value of 130-140 ℃·month; 3) the tropical region is limited in a very small area in southeastern Hainan Island and southern edge of Taiwan Island; and 4) considering a significant difference in climates between the southern and northern parts of the warm temperate zone, we suggested that the warm temperate zone in China is divided into two vegetation regions, deciduous broadleaf woodland region and deciduous and evergreen broadleaf mixed forest region, the Qinling Mountain_Huaihe River line being as their boundary. We also claimed that the zonal vegetation in North China is deciduous broadleaf woodland. Finally, we emphasized the importance of dynamic vegetation regionalization linked to climate changes. 展开更多
关键词 China dynamic vegetation regionalization arid/humid climate northern limit of subtropical zone Qinling Mountain_Huaihe River line thermal climate vegetation_climate relationship vegetation regionalization vegetation zone
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The Application of Pearson Correlational Analysis Method in Air Quality Analysis of Beijing-Tianjin-Hebei Region 被引量:15
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作者 杨宁 冀德刚 李双金 《Agricultural Science & Technology》 CAS 2015年第3期590-592,共3页
Based on the analysis of monitoring data on six pollution indexes of SO2, NO2, CO, O3, PM10 and PM2.5 from 53 monitoring points in 7 cities, including Beijing, Tianjin, Shijiazhuang, etc., from April 8 of 2014 to July... Based on the analysis of monitoring data on six pollution indexes of SO2, NO2, CO, O3, PM10 and PM2.5 from 53 monitoring points in 7 cities, including Beijing, Tianjin, Shijiazhuang, etc., from April 8 of 2014 to July 23 of 2014, this article adopted Pearson correlation coefficient method to determine the relevance among each pollutant of these cities with the help of SPSS. The results showed that such three leading indexes as SO2, PM10 and PM2.5 had strong correlation in Beijing, Tianjin and main cities of Hebei. Finally, some suggestions and preventive measures for the cooperative governance of air pollution in Beijing-Tianjin-Hebei Region were put forward, hoping this can help them. 展开更多
关键词 Pearson Correlation coefficient Linear interpolation Monitoring indicators Air quality
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Thesaurus-based approach for building domain ontology with a case study of military aircraft prototype ontology construction 被引量:3
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作者 真溱 唐爱民 +1 位作者 沈钧毅 卢胜军 《Journal of Southeast University(English Edition)》 EI CAS 2006年第3期353-356,共4页
To alleviate the amount of work involved in constructing a domain ontology, starting with the base of an existing terminological-rich thesaurus is better than starting from scratch. With a case study of reengineering ... To alleviate the amount of work involved in constructing a domain ontology, starting with the base of an existing terminological-rich thesaurus is better than starting from scratch. With a case study of reengineering the Defense Science and Technology Thesaurus into a prototype military aircraft ontology, a four-phase thesaurus-based methodology is introduced and investigated, which consists of identifying the application purpose, overall design, designing in detail and evaluation. Designing in detail is the core step, converting the terms and semantic relationships of the thesaurus into an ontology and supplementing richer semantic relationships. The resulting prototype ontology includes 87 concepts and 34 relationships, and can be extended and scaled up to a full-fledged domain ontology in the future. Eight universal genres of relationships of this ontology are preliminarily summarized and analyzed, including equivalent relationships, approximate relationships, generic/abstract relationships, part/whole relationships, cause/effect relationships, entity/location relationships etc., and the normalization of semantic relationships is critical to the merging and reusing of follow-up multiple ontologies. 展开更多
关键词 THESAURUS domain ontology CONSTRUCTION METHODOLOGY semantic relationship
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The Phylogenetic Relationships of an Endemic Genus Sinadoxa in the Qinghai-Xizang Plateau: Evidence from ITS Sequence Analysis 被引量:7
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作者 刘建全 陈之端 路安民 《Acta Botanica Sinica》 CSCD 2000年第6期656-658,共3页
对青藏高原特有的濒危植物华福花 (SinadoxacorydalifoliaC .Y .Wu ,Z .L .WuetR .F .Huang)的核糖体DNA中的内转录间隔区 (ITS)序列及 5 .8SrRNA基因的序列进行了测定。同川续断目和五加目有关类群序列的比较及分支分析表明华福花属与... 对青藏高原特有的濒危植物华福花 (SinadoxacorydalifoliaC .Y .Wu ,Z .L .WuetR .F .Huang)的核糖体DNA中的内转录间隔区 (ITS)序列及 5 .8SrRNA基因的序列进行了测定。同川续断目和五加目有关类群序列的比较及分支分析表明华福花属与五福花属 (AdoxaL .)近缘 ,不支持它可能与五加目或败酱科有亲缘关系的假设。尽管形态上它与五福花属分化十分明显 ,但ITS碱基分异却较小。引起其系统位置发生争论的外部形态为什么进化得如此之快 ,是值得进一步深入探讨的课题。 展开更多
关键词 Sinadoxa corydalifolia internal transcribed spacers (ITS) SYSTEMATICS EVOLUTION
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Morphology and crystallography of β precipitate phase in Mg-Gd-Y-Nd-Zr alloy 被引量:2
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作者 李婷 杜志伟 +5 位作者 张奎 李兴刚 袁家伟 李永军 马鸣龙 石国梁 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2012年第12期2877-2882,共6页
Morphology and crystal structure of β precipitate phase in Mg-7Gd-5Y-1Nd-0.5Zr (mass fraction, %) alloy were characterized by optical microscopy, scanning electron microscopy and transmission electron microscopy. Com... Morphology and crystal structure of β precipitate phase in Mg-7Gd-5Y-1Nd-0.5Zr (mass fraction, %) alloy were characterized by optical microscopy, scanning electron microscopy and transmission electron microscopy. Compositions were determined for β phase using thin foil energy dispersive spectroscopy. Precipitation at 400 ℃ involves formation of platelet and block-shaped β phase. The orientation relationship is and between β precipitate phase and α-Mg matrix with habit planes parallel to , and a composition of Mg5(Y0.4Gd0.4Nd0.2) is suggested for the β phase in Mg-7Gd-5Y-1Nd-0.5Zr alloy. 展开更多
关键词 Mg-Gd-Y-Nd-Zr alloy precipitation phase microstructure composition orientation relationship habit plane
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Analysis of Genetic Relationship among Medicago sativa,Medicago falcate and Trigonella foenum-graecum Using ISSR 被引量:2
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作者 刘磊 王宗礼 +4 位作者 李志勇 周国栋 师文贵 李鸿雁 蔡丽艳 《Agricultural Science & Technology》 CAS 2012年第10期2076-2079,共4页
[Objective] This study aimed to analyze the genetic relationships among Medicago sativa,Medicago falcata and Trigonella foenum-graecum.[Method] ISSR technique was adopted to determine their genetic relationships.[Resu... [Objective] This study aimed to analyze the genetic relationships among Medicago sativa,Medicago falcata and Trigonella foenum-graecum.[Method] ISSR technique was adopted to determine their genetic relationships.[Result] M.sativa,M.falcate and T.foenum-graecum had a broad genetic base.T.foenum-graecum shared closer relationship with M.falcata rather than M.sativa.The study on relationship between M.sativa and T.foenum-graecum was advantageous to identify disputable transition types.But a boundary should be found to identify species to be M.sativa or T.foenum-graecum.[Conclusion] This study will provide reference for identifying some disputable transition types. 展开更多
关键词 Medicago sativa L. Medicago falcata L. Trigonella foenum-graecum L. RELATIONSHIP ISSR
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Application of PCA and HCA to the Structure-Activity Relationship Study of Fluoroquinolones 被引量:2
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作者 李小红 张现周 +2 位作者 程新路 杨向东 朱遵略 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 北大核心 2006年第2期143-148,共6页
Density functional theory (DFT) was used to calculate molecular descriptors (properties) for 12 fluoro-quinolone with anti-S.pneumoniae activity. Principal component analysis (PCA) and hierarchical cluster analy... Density functional theory (DFT) was used to calculate molecular descriptors (properties) for 12 fluoro-quinolone with anti-S.pneumoniae activity. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) were employed to reduce dimensionality and investigate in which variables should be more effective for classifying fluoroquinolones according to their degree of an-S.pneumoniae activity. The PCA results showed that the variables ELUMO, Q3, Q5, QA, logP, MR, VOL and △EHL of these compounds were responsible for the anti-S.pneumoniae activity. The HCA results were similar to those obtained with PCA.The methodologies of PCA and HCA provide a reliable rule for classifying new fluoroquinolones with antiS.pneumoniae activity. By using the chemometric results, 6 synthetic compounds were analyzed through the PCA and HCA and two of them are proposed as active molecules with anti-S.pneumoniae, which is consistent with the results of clinic experiments. 展开更多
关键词 Structure-activity relationship Density functional theory Principal component analysis Hierarchical cluster analysis
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Polymerization Mechanism of α-Linear Olefin 被引量:3
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作者 邢文国 张长桥 +2 位作者 于萍 刘成卜 魏云鹤 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2010年第1期39-44,I0001,共7页
The density functional theory on the level of B3LYP/6-31G was empolyed to study the chain growth mechanism in polymerization process of α-linear olefin in TiCl3/AlEt2Cl catalytic system to synthesize drag reduction a... The density functional theory on the level of B3LYP/6-31G was empolyed to study the chain growth mechanism in polymerization process of α-linear olefin in TiCl3/AlEt2Cl catalytic system to synthesize drag reduction agent. Full parameter optimization without symmetry restrictions for reactants, products, the possible transition states, and intermediates was calculated. Vibration frequency was analyzed for all of stagnation points on the potential energy surface at the same theoretical level. The internal reaction coordinate was calculated from the transition states to reactants and products respectively. The results showed as flloes: (i) Coordination compounds were formed on the optimum configuration of TiCl3/AlEt2Cl.(ii) The transition states were formed. The energy di?erence between transition states and the coordination compounds was 40.687 kJ/mol. (iii) Double bond opened and Ti-C(4) bond fractured, and the polymerization was completed. The calculation results also showed that the chain growth mechanism did not essentially change with the increase of carbon atom number of α-linear olefin. From the relationship between polymerization activation energy and carbon atom number of the α-linear olefin, it can be seen that the α-linear olefin monomers with 6-10 carbon atoms had low activation energy and wide range. It was optimum to synthesize drag reduction agent by polymerization. 展开更多
关键词 Density functional theory Polymerization mechanism α-Linear olefin Drag reduction agent Ziegler-Natta catalyst
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Assessment of Genetic Variability and Inter-specific Relationships among Four Species of Groupers (Genus Epinephelus) from South China Sea Using Microsatellite Markers 被引量:2
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作者 尹绍武 黄海 +1 位作者 骆剑 胡亚丽 《Agricultural Science & Technology》 CAS 2012年第2期428-433,共6页
[Objective] The study aimed to evaluate the genetic variability and interspecific relationships among four species of groupers from South China Sea, including E. fario, E. merra, E. malabaricus and E. coioides. [Metho... [Objective] The study aimed to evaluate the genetic variability and interspecific relationships among four species of groupers from South China Sea, including E. fario, E. merra, E. malabaricus and E. coioides. [Method] Twenty one mircosatellite loci of groupers were selected from GenBank and eight high polymorphic loci were used to further genetic analysis. [Result] The mean number of alleles per locus (A), effective number of alleles (Ne), mean polymorphism information content (PIC), observed heterozygosity (Ho) and expected heterozygosity (He) were 4.38±1.60, 3.69±0.86, 0.69±0.08, 0.67±0.08, 0.72±0.06 in E. malabaricus; 3.88±1.13, 3.55±1.04, 0.66±0.10, 0.68±0.21, 0.70±0.08 in E.coioides; 6.00±1.07, 4.68±0.65, 0.78±0.03, 0.73±0.25, 0.79±0.03 in E. fario; 5.50±1.07, 4.58±0.80, 0.76±0.05, 0.75±0.18, 0.78±0.04 in E. merra, respectively. [Conclusion] We compared the values above, the order of the genetic variability among these grouper species was E. fario E. merra E. malabaricus E. coioides. We found that the level of genetic variability of these groupers species was relatively higher than that of other marine fish, so their genetic status was good. In addition, the analyisis of genetic relationship showed that E. malabaricus and E. coioides was the closest and it was the farthest between E. merra and E. coioides. 展开更多
关键词 GROUPER EPINEPHELUS Genetic variability Species relationships Microsatellite marker
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Selection Model in Buyer-Supplier Relationships 被引量:2
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作者 孙晓林 罗定提 +1 位作者 黄国全 仲伟俊 《Journal of Southeast University(English Edition)》 EI CAS 2002年第4期346-350,共5页
This paper proposes a new method of selecting appropriate buyer supplier relationships (BSR) for specific projects. Because it is almost impossible in reality to establish mathematical relationships between the BSR at... This paper proposes a new method of selecting appropriate buyer supplier relationships (BSR) for specific projects. Because it is almost impossible in reality to establish mathematical relationships between the BSR attributes and the factors of a project, the concept of relationship indices (RI) is introduced to quantify such BSR which are in turn established through design of experiments. Based on the experimental results, the contributions of project factors, known as factors relationship worths (RW... 展开更多
关键词 supply chain buyer supplier relationship experimental design
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Change Trend of Chinese Urban Residents' Food-nitrogen Consumption 被引量:2
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作者 王俊能 许振成 彭晓春 《Agricultural Science & Technology》 CAS 2010年第4期176-179,共4页
Change trend of Chinese urban residents' per capita food-nitrogen annual consumption from 1981 to 2007 was analyzed and predicted by using ARIMA time-series model in order to reveal the change of urban food-nitrogen ... Change trend of Chinese urban residents' per capita food-nitrogen annual consumption from 1981 to 2007 was analyzed and predicted by using ARIMA time-series model in order to reveal the change of urban food-nitrogen consumption during the China's urbanization process.Results showed that after 1980s,the annual consumption of Chinese urban residents' food-nitrogen had a change trend of " increase-decrease-increase" and generally presented as a slight increasing trend;With the acceleration of rapid economic development and urbanization process,Chinese urban residents' food-nitrogen consumption will still keep a rising trend in future,and also has a large rising space. 展开更多
关键词 Food consumption NITROGEN Spearman's rank correlation coefficient ARIMA model Urban residents China
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Semantic-based query processing for relational data integration 被引量:1
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作者 苗壮 张亚非 +2 位作者 王进鹏 陆建江 周波 《Journal of Southeast University(English Edition)》 EI CAS 2011年第1期22-25,共4页
To solve the query processing correctness problem for semantic-based relational data integration,the semantics of SAPRQL(simple protocol and RDF query language) queries is defined.In the course of query rewriting,al... To solve the query processing correctness problem for semantic-based relational data integration,the semantics of SAPRQL(simple protocol and RDF query language) queries is defined.In the course of query rewriting,all relative tables are found and decomposed into minimal connectable units.Minimal connectable units are joined according to semantic queries to produce the semantically correct query plans.Algorithms for query rewriting and transforming are presented.Computational complexity of the algorithms is discussed.Under the worst case,the query decomposing algorithm can be finished in O(n2) time and the query rewriting algorithm requires O(nm) time.And the performance of the algorithms is verified by experiments,and experimental results show that when the length of query is less than 8,the query processing algorithms can provide satisfactory performance. 展开更多
关键词 data integration relational database simple protocol and RDF query language(SPARQL) minimal connectable unit query processing
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Intelligent method to develop constitutive relationship of Ti-6Al-2Zr-1Mo-1V alloy 被引量:1
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作者 孙宇 曾卫东 +2 位作者 赵永庆 韩远飞 马雄 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2012年第6期1457-1461,共5页
The isothermal compression tests were carried out in the Thermecmastor-Z thermo-simulator at temperatures of 800, 850, 900, 950, 1000 and 1050 ℃ and the strain rates of 0.01, 0.1, 1 and 10 s-1. The influence of defor... The isothermal compression tests were carried out in the Thermecmastor-Z thermo-simulator at temperatures of 800, 850, 900, 950, 1000 and 1050 ℃ and the strain rates of 0.01, 0.1, 1 and 10 s-1. The influence of deformation temperature and strain rate on the flow stress of Ti-6Al-2Zr-IMo-IV alloy was studied. Based on the experimental data sets, the high temperature deformation behavior of Ti-6A1-2Zr-IMo-IV alloy was presented using the intelligent method of artificial neural network (ANN). The results indicate that the predicted flow stress values by ANN model is quite consistent with the experimental results, which implies that the artificial neural network is an effective tool for studying the hot deformation behavior of the present alloy. In addition, the development of graphical user interface is implemented using Visual Basic programming language. 展开更多
关键词 Ti-6A1-2Zr-1Mo-IV alloy artificial neural network constitutive relationship deformation behavior
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Human tracking in camera network with non-overlapping FOVs 被引量:2
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作者 林国余 杨彪 张为公 《Journal of Southeast University(English Edition)》 EI CAS 2012年第2期156-163,共8页
An adaptive human tracking method across spatially separated surveillance cameras with non-overlapping fields of views (FOVs) is proposed. The method relies on the two cues of the human appearance model and spatio-t... An adaptive human tracking method across spatially separated surveillance cameras with non-overlapping fields of views (FOVs) is proposed. The method relies on the two cues of the human appearance model and spatio-temporal information between cameras. For the human appearance model, an HSV color histogram is extracted from different human body parts (head, torso, and legs), then a weighted algorithm is used to compute the similarity distance of two people. Finally, a similarity sorting algorithm with two thresholds is exploited to find the correspondence. The spatio- temporal information is established in the learning phase and is updated incrementally according to the latest correspondence. The experimental results prove that the proposed human tracking method is effective without requiring camera calibration and it becomes more accurate over time as new observations are accumulated. 展开更多
关键词 multiple camera tracking non-overlapping FOVs spatio-temporal information human appearance model incremental learning
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Optimization of ISSR-PCR Reaction System and Preliminary Construction of ISSR Fingerprinting of Some Species in Bryaceae 被引量:3
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作者 汪琛颖 赵建成 《Agricultural Science & Technology》 CAS 2011年第11期1561-1564,1568,共5页
[Objective] The aim was to provide molecular basis for the identification of species in the moss family Bryaceae by the construction of inter-simple sequence repeats (ISSR) fingerprinting. [Method] In order to seek ... [Objective] The aim was to provide molecular basis for the identification of species in the moss family Bryaceae by the construction of inter-simple sequence repeats (ISSR) fingerprinting. [Method] In order to seek standardizing PCR reaction set-up, an orthogonal design was used to optimize ISSR-PCR amplification system of Bryaceae in five factors (Mg2+, dNTPs, primer, DNA template, Taq DNA polymerase) at four levels respectively. [Result] A suitable ISSR reaction system was obtained, namely: 20 μl reaction system containing 5 ng of DNA template, 0.2 μmol/L primer, 2.25 mmol/L MgCl2, 0.6 U of Taq DNA polymerase, 0.4 mmol/L dNTPs. Proper annealing temperature was found at 48-50 ℃.The above system and six ISSR-PCR primers were used for the PCR amplification of 14 samples from Bryaceae and the related species in Mniaceae. A total of 86 bands were amplified, all showed polymorphism. NJ cluster analysis showed a star-shaped cladogram. [Conclusion] The results manifested that ISSR fingerprinting could provide the appropriate degree of polymorphism at low taxonomic level, so it would be a useful tool to provide additional evidence for resolving taxonomic relationships at the species level of Bryaceae. 展开更多
关键词 BRYACEAE ISSR Optimization of reaction system Species-level Taxonomic relationship
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Hot compressive behavior of Ti-3.0Al-3.7Cr-2.0Fe-0.1B titanium alloy 被引量:1
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作者 王国 惠松骁 +1 位作者 叶文君 米绪军 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2012年第12期2965-2971,共7页
The hot deformation behavior of Ti-3.0Al-3.7Cr-2.0Fe-0.1B (TACFB) titanium alloy was investigated using a Gleeble-1500D thermal simulator in the temperature range of 800-950 °C, at constant strain rate from 0.01 ... The hot deformation behavior of Ti-3.0Al-3.7Cr-2.0Fe-0.1B (TACFB) titanium alloy was investigated using a Gleeble-1500D thermal simulator in the temperature range of 800-950 °C, at constant strain rate from 0.01 s-1 to 10 s-1 and with height reduction of 70%. Flow stress and microstructure evolution during hot compression of TACFB alloy were investigated. The processing map of TACFB alloy was obtained. The results indicate that the hot deformation behavior of TACFB alloy is sensitive to the deformation temperature and strain rate. The peak flow stress decreases with increasing the test temperature and decreasing the strain rate. The constitutive relationship of TACFB alloy was obtained on the base of Arrhenius equations. When the strain rates are higher than 1.0 s-1, the dynamic recrystallization occurs, and the higher the strain rates are, the more the recrystallization is. 展开更多
关键词 titanium alloy Ti-3.0Al-3.7Cr-2.0Fe-0.1B alloy hot compressive constitutive relationship processing map dynamic recrystallization
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Electronic Structures, DNA-binding, SAR, and Spectral Properties of Ruthenium Methylimidazole Complexes [Ru(MeIm)4L]^2+(L=iip,tip,2ntz) 被引量:1
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作者 李国栋 陈兰美 +3 位作者 王忻宇 巫玲凤 揭新明 陈锦灿 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2014年第2期159-167,I0003,共10页
Theoretical studies on the electronic and geometric structures, the trend in DNA-binding affinities as well as the the structure-activity relationship (SAR) of a series of water-soluble Ru(II) methylimidazole comp... Theoretical studies on the electronic and geometric structures, the trend in DNA-binding affinities as well as the the structure-activity relationship (SAR) of a series of water-soluble Ru(II) methylimidazole complexes, i.e. [Ru(Mehn)4iip]^2+ (1) (MeIm=l-methylimidazole, iip=2-(1H-imidazo-4-group)-lH-imidazo[n,5-f][1,10]phenanthroline), [Ru(MeIm)4tip]^2+ (2) (tip=2-(thiophene-2-group)-lH-imidazo[4,5-f] [i,10]phenanthroline), and [Ru(Melm)42ntz]^2+ (3) (2ntz=2-(2-nitro-l,3-thiazole-5-group)-lH-imidazo[4,5-f][1,10]phenanthroline), were car- ried out using the density functional theory (DFT). The electronic structures of these Ru(II) complexes were analyzed on the basis of their geometric structures optimized in aqueous solution, and the trend in the DNA-binding constants (Kb) was reasonably explained. The results show that the replacement of imidazole ligand by thiophene ligand can effectively improve the DNA-binding affinity of the complex. Meanwhile, it was found that introduc- ing the stronger electronegative N atom and NO2 group on terminal loop of intercalative ligand can obviously reduce the complex's LUMO and HOMO-LUMO gap energies. Based on these findings, the designed complex [Ru(MeIm)42ntz]^2+ (3) can be expected to have the greatest Kb value in complexes 1-3. In addition, the structure-activity relationships and antitumor mechanism were also carefully discussed, and the antimetastatic activity of the designed complex 3 was predicted. Finally, the electronic absorption spectra of this series of complexes in aqueous solution were calculated, simulated and assigned using DFT/TDDFT methods as well as conductor-like polarizable continuum model (CPCM), and were in good agreement with the experimental results. 展开更多
关键词 Ruthenium methylimidazole complex DNA-BINDING Structure-activity rela- tionships Spectral property DFT calculation
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