Influence of Climate and Daylength on the Activity Budgets of Feral Goats (Capra hircus) on the Isle of Rum, Scotland

Daytime activity budgets of feral goats （ Capra hircus ） were studied in 1981, 1982 and 2000 on the Isle of Rum, northwest Scotland. This paper analyzes the influence of key weather variables （temperature and rainfall） and seasonal variations in daylength on daytime activity budgets. This study showed that the percentage of daytime spent in feeding by feral goats on Rum Island decreased with average monthly temperature, while the percentage of daytime spent in lying increased and rainfall had no significant effects on the percentage daytime spent in feeding or lying. The daylength varied greatly from January to December with the longest daylength being in June and July, and the shortest one in December and January. The amount of daytime spent feeding decreased from summer to winter, although the percentage of daytime spent feeding increased greatly from summer to winter. The seasonal variations in daylength seem to be an important factor in constraining feral goats＇ activity, especially in the cold and wet winters when forage biomass and quality are both at their lowest level. We discuss the influence of such constraining effects on the forage intake of these goats and their winter survival rate, as well as its implication for population regulation of these goats.

Study on complexity of plant communities at different altitudes on the Northern Slope of Changbai Mountain

By the method of gradient pattern analysis, twenty plots were set at altitudes of 700-2600 m with an interval of 100 m on the northern slope of the Changbai Mountain. The dissimilarity of respective sub-plots in the same community was measured and the complexity of plant communities at different altitudes was analyzed. The result from binary data of tree species in canopy tree indicated that the sub-plots in the communities, except subalpine Betula ermanii forest, showed comparatively high dissimilarity in species composition. Especially, the dissimilarity index (0.7) of broadleaved/Korean pine forest at low altitudes was obviously higher than other communities. The differences are not obvious between communities referring to dark coniferous forest. Comparatively, the dissimilarity in sub-plots of the communities at altitude of 1400 m was slightly higher than that of other communities, which reflected the complexity of tree species compositions of transitory-type communities. For subalpine Betula ermanii forest, tree species composition was simple and showed a high similarity between sub-plots. The results derived from binary data of shrub showed that the dissimilarity index of shrub species in broadleaved/Korean pine forest at low altitudes was higher than that in other communities, but the divergence tendency wasn抰 so obvious as that of arbor species. The dissimilarity derived from binary data of herb and all plant species at different altitudes showed greatly close tendency, and the differences in herb and all plant species between sub-plots were the greatest for the communities of broad-leaved-Korean pine forest and alpine tundra zone..

Effects of Temperature on Determination of Protein Concentration with Coomassie Brilliant Blue Method

[Objective] This study aimed to investigate the effects of temperature on determination of protein concentration with Coomassie Brilliant Blue method,thus proving advice and guidance for accurate determination of protein concentration.[Method] With Coomassie Brilliant Blue method,the concentrations of different bovine serum albumin samples were determined under different temperatures and incubation time.[Result] According to the standard curve,when the determination range of protein concentration was 0-100 mg/ml,the determined protein concentration was relatively stable after incubation at 20 ℃ for 20-30 min.Furthermore,the determination result of higher protein concentration with Coomassie Brilliant Blue method was less affected by various factors.[Conclusion] In determination of protein concentration with Coomassie Brilliant Blue method,temperature,sample concentration and incubation time were important factors affecting the accuracy of experimental results.

The changes of co-possession of plant species between communities with altitudes on northern slope of Changbai Mountain

Twenty plots were investigated on northern slope of Changbai Mountain at an attitude interval of 100 m (from 700 to 2600 m). The species co-possession between plant communities at different altitudes was analyzed by Jaccard index. The analytical results showed that the co-possession calculated according to species in different layers or all species between adjacent communities was higher than that between disjunct communities. The co-possessions between adjacent communities calculated by species in different layers had comparability and dissimilarity. If the two adjacent communities belong to different types of vegetation, then their co-possession was lower. The peak values and valley values of species co-possession between communities along elevation gradient just matched vegetation gradient patterns, and species subrogation of shrubs had more obvious rule on northern slope of Changbai Mountain. Co-possessions between communities with same altitude difference were much similar, and it decreased as the increase of elevation difference, which showed that species compositions in different layers of the communities were highly related.

Dynamics of profiles and storage of carbon dioxide in broadleaved/Korean forest in Changbai Mountain

CO2 concentrations at different heights in a broadleaved/Korean forest (with a mean height of 26 m) were measured with infrared gas analyzer IRGA (model 2250D, LI-COR Inc. and LI-COR, 820) from Aug. to Oct. of 1999, Apr. to Jul. of 2000, and from Aug. 2002 to Sept. 2003. Based on the collected dada, the diurnal and seasonal dynamics of profiles and storage of carbon dioxide in the forest were analyzed. The diurnal CO2 profiles showed that the vertical distribution of CO2 concentration were different for daytime and nighttime, and the CO2 concentration was highest close to forest floor, no matter at daytime and nighttime. The seasonal profiles of CO2 showed that stratification in the canopy was evident during growth season. CO2 concentrations at different heights (60 m to 2.5 m) had a little change in March, with a difference of 10 mmolmol-1, but had a significant change in July, with a difference of 60 mmolmol-1. In July, there also existed a greater gradient of CO2 concentrations at canopy (22, 26 and 32 m), with a difference of 8 mmolmol-1. The calculated total storage (ΔC/Δt ) of CO2 in the air column with height of 40 m beneath eddy covariance instrument was negative, and made a little contribution to NEE.

Effects of Salinity Fluctuation and Dietary Traditional Chinese Herbal Medicines on Survival,Growth and Immunity of Exopalaemon carinicauda

[Objective]The aim of this study was to provide a theoretical basis for the large-scale breeding of Exopalaemon carinicauda. [Method]Effects of salinity fluctuation （S0,S3,S6 and S9 represented salinity fluctuations of 0,3,6 and 9 respectivley） and dietary traditional Chinese herbal medicines （0,1%,2% and 4% respectively） on survival,growth and immunity of E.carinicauda were studied,and its experiment lasted for 60 d. [Result]The results showed that specific growth rate,feed conversion efficiency and superoxide dismutase activity of E.carinicauda in the treatment group with 1% dietary traditional Chinese herbal medicine and S6 salinity fluctuation were higher than those in other treatments,which was significantly higher than the treatment groups with S0,S3 and S9 salinity fluctuation at the same adding level of 1% dietary traditional Chinese herbal medicine. The regression analysis indicated that at S0,S3 and S6 salinity fluctuation,the optimal adding level of dietary traditional Chinese herbal medicines in feeds decreased with the increase of salinity fluctuation,accounting for 2.74%,1.77% and 0.51% respectively. At four adding levels of dietary traditional Chinese herbal medicines,the optimal salinity fluctuation of E.carinicauda also decreased with the increase of dietary traditional Chinese herbal medicines in feeds,accounting for 5.26,4.95,3.54 and 1.97 respectively. [Conclusion]Salinity,dietary traditional Chinese herbal medicines and their interactions have a significant effect on survival,growth and immunity of E.carinicauda,which calls for attentions in the breeding.

Effects of soil temperature and soil water content on soil respiration in three forest types in Changbai Mountain

Soil incubation experiments were conducted in lab to delineate the effect of soil temperature and soil water content on soil respirations in broad-leaved/Korean pine forest (mountain dark brown forest soil), dark coniferous forest (mountain brown coniferous forest soil) and erman's birch forest (mountain soddy forest soil) in Changbai Mountain in September 2001. The soil water content was adjusted to five different levels (9%, 21%, 30%, and 43%) by adding certain amount of water into the soil cylinders, and the soil sample was incubated at 0, 5, 15, 25 and 35°C for 24 h. The results indicated that in broad-leaved/Korean pine forest the soil respiration rate was positively correlated to soil temperature from 0 to 35°C. Soil respiration rate increased with increase of soil water content within the limits of 21% to 37%, while it decreased with soil water content when water content was over the range. The result suggested the interactive effects of temperature and water content on soil respiration. There were significant differences in soil respiration among the various forest types. The soil respiration rate was highest in broad-leaved/Korean pine forest, middle in erman's birch forest and the lowest in dark coniferous forest. The optimal soil temperature and soil water content for soil respiration was 35°C and 37% in broad-leaved/Korean pine forest, 25°C and 21% in dark coniferous forest, and 35°C and 37% in erman's birch forest. Because the forests of broad-leaved/Korean pine, dark coniferous and erman's birch are distributed at different altitudes, the soil temperature had 4–5°C variation in different forest types during the same period. Thus, the soil respiration rates measured in brown pine mountain soil were lower than those in dark brown forest and those measured in mountain grass forest soil were higher than those in brown pine mountain soil. Key words Soil temperature - Soil water content - Soil respiration - The typical forest ecosystem in Changbai Mountain CLC number S7118.51 Document code A Foundation item: This study was supported by grant from the National Natural Science Foundation of China (No. 30271068), the grant of the Knowledge Innovation Program of Chinese Academy of Sciences (KZ-CX-SW-01-01B-12) and the grant from Advanced Programs of Institute of Applied Ecology Chinese Academy of Sciences.Biography: WANG Miao (1964-), male, associate professor in Institute of Applied Ecology, Chinese Academy of Science, Shenyang 110016, P. R. China.Responsible editor: Song Funan

二组分混合纤维定量分析新方法的探讨

探索着色剂法定量分析二组分混合纤维层的可行性。分别称量10个不同比例的涤、棉纤维,经梳棉机混合梳理后对其进行染色得到其颜色随着棉含量增加而渐深的涤棉纤维层,实验数据表明涤/棉混合纤维层在染色后的△白度与其棉比例的线性关系较好,相关系数达0.98。

The effect of exogenous sugar solution and high concentration of CO_2 on the contents of sugar and protein of Betula platyphylla leaves

The content of total sugar, sucrose, fructose and protein in the leaves of3-yr.-old Betula platyphylla was measured after the treatment by three exogenous sugar solutions(sucrose, fructose, glucose) and three high concentrations of CO_2 (700, 1 400, 2 100 μL/L·L^(-1))for about a month in 1998. The results showed that spraying three exogenous sugar solutionsincreased markedly the content of sugar and protein of leaves under 700 μL·L^(-1) and 1 400μL·L^(-1) CO_2 The effect of spraying exogenous sucrose solution was the best among the threeexogenous sugars. The treatment of spraying exogenous sugar solution and 2 100 μL·L^(-1) CO_2constrained the accumulation of total sugar and protein of leaves. There was no difference inprotein content of leaves when spraying glucose and fructose solutions under 700 μL·L^(-1) and 1400 μL·L^(-1) CO_2. The treatment of 2 100 μL·L^(-1) CO_2 concentration significantly increasedthe contents of total sugar, sucrose, fructose, and protein of leaves compared with that of the 700μL·L^(-1) and 1 400 μL·L^(-1) CO_2 except the plants spraying fructose solution. There waspositive correlation between the content of sugar of leaves and CO_2 concentration when sprayingsame exogenous sugar solution.

The Effects of Apolipoprotein E Polymorphism on Serum Lipids, Lipoproteins and Apolipoproteins Variation

Objective: To study the effects of Apolipoprotein E (ApoE) polymorphism onserum levels of lipids, lipoproteins and apolipoproteins. Methods: Fragments of ApoE gene forthex-on containing codon 112 and 158 polymorphic locus were amplified by PCR, and then digested untilCfo I endonuclease. Genotypes and alleles frequencies of 168 healthy persons in Jiangsu area werecalculated. The effects of ApoE genotypes and alleles on serum lipids, lipoproteins andapolipoproteins variation were analyzed. Results: The effects of ApoE alleles on total cholesterol(TC), law density lipoprotein-cholesterol (LDL-C), ApoB was: along a decreasing gradientε_4>ε_3>ε_2. The effect of ε_4 allele was to increase serum levels of TC, LDL-C and ApoB, andthe ε_2 allele had an effect opposite to that of ε_4 allele. Conclusion: ApoE polymorphism is anindependent genetic factor on individual serum levels of lipids and apolipoproteins.

Effects of salinity fluctuation and dietary protein/ carbohydrate ratio on the survival and growth of Juvenile Litopenaeus vannamei

A factorial study was conducted to determine the effects of salinity （constant （SO） and fluctuating （S10）） and isoenergetic diet of different protein/carbohydrate ratios （P/C： 4.1, 1.9, 1.0 and 0.6） on the survival and growth of juvenile Litopenaeus vannamei. The experiment lasted for 35 days. The results showed that I） At S0 and S10, specific growth rate and apparent digestibility coefficient exhibited decreasing trend, while food conversion ratio exhibited increasing trend, with decreasing P/C ratio. At SO, food consumption and specific growth rate of the shrimp fed 4.1 P/C were significantly higher than those fed 1.0 and 0.6 P/C, whereas at S10, no significant differences were detected among different treatments; II） At 4.1 and 1.9 P/C, food consumption and specific growth rate were higher in the shrimp maintained at SO as compared with those at S10, while at 1.0 and 0.6 P/C, an inverse trend was observed. Among four P/C ratios, food conversion ratio of the shrimp at SO was the lowest.

电针对APP/PS1转基因小鼠海马低密度脂蛋白受体相关蛋白-1及其mRNA表达的影响

目的:研究电针是否是通过影响LRP1mRNA水平、提高LRP1的表达,从而促进脑内Aβ清除。方法:将4月龄APP/PS1转基因鼠,随机分为模型组、电针治疗组,以同窝同背景转基因阴性小鼠为正常对照组。电针干预"涌泉"、"百会"0.1 m A,15 min/次,隔日1次,治疗6周。治疗后,以免疫组化法观察脑组织LRP1阳性表达,以Western blotting法检测海马LRP1表达,以Real-time PCR法检测海马LRP1mRNA表达。结果:LRP1表达于脑微血管内皮细胞、胶质细胞、神经元等处。模型组海马LRP1蛋白、LRP1mRNA相对表达量低于正常对照组(P<0.05),电针治疗组海马LRP1及LRP1mRNA比模型组有上升趋势,但差异无统计学意义(P>0.05)。结论:电针干预可能影响5月龄APP/PS1转基因小鼠海马内所有细胞LRP1及LRP1mRNA表达,可能是电针干预AD发病的潜在靶点,但实验方案还需进一步完善。

Investigation on the Sterility Changeover of Male Sterility Line CMS7311 in Heading Chinese Cabbage

Sterility changeover induced by low temperature in male sterility line CMS7311 of heading Chinese cabbage ( Brassica campestris L. ssp. penkinsis) was investigated under constant temperature, day/night alternating temperature in light-growth-incubator and outdoor natural day/night alternating temperature respectively. It showed that the sterility of CMS7311 has significant change with temperature. The constant temperature of 6 - 12 degreesC was suitable to induce the changeover of sterility (CGS) of CMS7311 ; the shortest treatment time for CGS induction at this temperature was 3 d. The significant value of sterility changeover of CMS7311 occurred at the constant temperature of 6 - 9 degreesC after treatment for 6 - 9 d. The intensity of CGS was stronger under constant temperature than under alternate temperature, but the shortest treatment time inducing CGS has no difference between them. When treated for 9 d under the day/night alternating temperature of 11/7 degreesC, its sterility could also turn to normal fertility (near to sterile grade I or zero). In normal condition, the interval from the end of temperature treatment to the beginning of CGS induction was 10 - 16 d. In the condition of natural day/night alternating temperature, the lower limit of temperature for effective CGS induction was at the daily mean temperature ( DMT) of 3. 5 - 6. 8 degreesC, and the shortest treatment time which could induce the sterility changeover was also longer than that under constant temperature, being 11d at DMT of 1.5 - 3.1 degreesC. In addition, for the apical dominance, the flower bud on the first lateral branch showed the CGS 8 - 10 d later than the flower bud on the main stem under the same temperature with the same days of treatment. The intensity of CGS of CMS7311 on the first lateral branch was also weaker than the flower bud on the main stem.

Density Functional Theory Calculations of Charge-Induced Spin Polarization in Pentacene

Based on density functional theory （DFT） calculations, we investigate the spin-related properties of spinless-hole injected organic molecule pentacene （Pc）. DFT calculations reveal that there is spontaneous spin polarization in Pc when spinless-hole is injected. The chargeinduced magnetic moment of Pc increases linearly with the increasing of the extra hole charge amount and its maximum can be up to 1 μB per injected spinless-hole per Pc molecule. The magnetic moment is expected due to the injected unpaired charge. The injected hole will preferably ll the spin-splitted carbon pz orbitals, which makes the Pc molecule spin polarize.

Lipoxygenase Activity and Its Relationship with Traits of Whole-mill Color in Wheat

130 wheat varieties are selected as materials to test the LOX activity, whiteness, yellowness, carotenoids content and RVA parameters, which are to be analyzed by variance analysis, correlation analysis, cluster analysis and classified according to the origins. The result shows there are highly significant variations in LOX activity from varieties; LOX activity is positively correlated with whiteness, and significantly and negatively correlated with yellowness. Cluster analysis applying longest distance method based on LOX activity clusters all the varieties or lines into three major groups. There are great differences between the averages of LOX activity from varieties. LOX activities are discussed in the application of nutritional quality improvement in wheat.

Turbulence regime near the forest floor of a mixed broad leaved/Korean pine forest in Changbai Mountains

The measurement and observation for this study were carried out by using a three-dimensional (u, v, w) Sonic anemometer (IAP-SA 485), at Forest Ecosystem Opened Research Station of Changbai Mountains (12828扙 and 4224?N, Jilin Province, P. R. China) in August 2001. The basic characteristics of turbulence, such as turbulence intensity, atmospheric stability, time scales, and convection state, near the forest floor were analyzed. It is concluded that the airflow near forest floor is characterized by high intermittence and asymmetry, and the active and upward movement takes the leading position. Near forest floor, the vertical turbulence is retained and its time scale and length scale are much less than that of u, v components. The eddy near forest floor shows a flat structure and look like a ’Disk’. Buoyancy plays a leading role in the generation and maintenance of local turbulence

Bio-solubilization of Chinese lignite I:extra-cellular protein analysis

A white rot fungus strain, Trichoderma sp.AH, was isolated from rotten wood in Fushun and used to study the mechanism of lignite bio-solubilization.The results showed that nitric acid pretreated Fushun lignite was solubilized by T.sp.AH and that extracellular proteins from T.sp.AH were correlated with the lignite bio-solubilization results.In the presence of Fushun lignite the extracellular protein concentration from T.sp.AH was 4.5 g/L while the concentration was 3 g/L in the absence of Fushun lignite.Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) of the extracellular proteins detected at least four new protein bands after the T.sp.AH had solubilized the lignite.Enzyme color reactions showed that extracellular proteins from T.sp.AH mainly consisted of phenol-oxidases, but that lignin decomposition enzymes such as laccase, peroxidase and manganese peroxidases were not present.

Atherosclerosis： from biology to pharmacological treatment

A recent explosion in the amount of cardiovascular risk has swept across the globe. Primary prevention is the preferred method to lower cardiovascular risk. Lowering the prevalence of obesity is the most urgent matter, and is pleiotropic since it affects blood pressure, lipid profiles, glucose metabolism, inflammation, and atherothrombotic disease progression. Given the current obstacles, success of primary prevention remains uncertain. At the same time, the consequences of delay and inaction will inevitably be disastrous, and the sense of urgency mounts. Pathological and epidemiological data confirm that atherosclerosis begins in early childhood, and advances seamlessly and inexorably throughout life. Risk factors in childhood are similar to those in adults, and track between stages of life. When indicated, aggressive treatment should begin at the earliest indication, and be continued for many years. For those patients at intermediate risk according to global risk scores, C-reactive protein, coronary artery calcium, and carotid intima-media thickness are available for further stratification. Using statins for primary prevention is recommended by guidelines, is prevalent, but remains under prescribed. Statin drugs are unrivaled, evidence-based, major weapons to lower cardiovascular risk. Even when low density lipoprotein cholesterol targets are attained, over half of patients continue to have disease progression and clinical events. Though clinical evidence is incomplete, altering or raising the blood high density lipoprotein cholesterol level continues to be pursued. The aim of this review is to point out the attention of key aspects of vulnerable plaques regarding their pathogenesis and treatment.

Metabolic syndrome and gallstone disease

AIM： To investigate the association between metabolic syndrome （NetS） and the development of gallstone disease （GSD）. METHODS： A cross-sectional study was conducted in 7570 subjects （4978 men aged 45.0：1：8.8 years, and 2592 women aged 45.3：1：9.5 years） enrolled from the physical check-up center of the hospital. The subjects included 918 patients with gallstones （653 men and 265 women） and 6652 healthy controls （4325 men and 2327 women） without gallstones. Body mass index （BII）, waist circumference, blood pressure, fasting plasma glucose （FPG） and serum lipids and lipoproteins levels were measured. Colorimetric method was used to measure cholesterol, high-density lipoprotein cho- lesterol （HDL-C） and low-density lipoprotein cholesterol （LDL-C）. Dextrose oxidizing enzyme method was used to measure FPG. Subjects were asked to complete a questionnaire that enquired about the information on demographic data, age, gender, histories of diabetes mellitus, hypertension, and chronic liver disease and so on. Metabolic syndrome was diagnosed according to the Adult Treatment Panel HI （ATP ]3） criteria. Gall- stones were defined by the presence of strong intralu- minal echoes that were gravity-dependent or attenu- ated ultrasound transmission. RESULTS： Among the 7570 subjects, the prevalence of the gallstone disease was 12.1% （13.1% in men and 10.2% in women）. BMI, waist circumference, systolic blood pressure, diastolic blood pressure, fasting blood glucose and serum triglyceride （TG） in cases group were higher than in controls, while serum high-density lipid was lower than in controls. There were significant differences in the waist circumference, blood pressure, FPG and TG between cases and controls. In an age- adjusted logistic regression model, metabolic syndrome was associated with gallstone disease. The age-adjust- ed odds ratio of MetS for GSD in men was 1.29 [95% confidence interval （CI）, 1.09-1.52; P = 0.0030], and 1.68 （95% CI, 1.26-2.25; P = 0.0004） in women; the overall age-adjusted odds ratio of NetS for GSD was 1.42 （95% CI, 1.23-1.64; P 〈 0.0001）. The men with more metabolic disorders had a higher prevalence of gallstone disease, the trend had statistical significance （P 〈 0.0001）. The presence of 5 components of the MetS increased the risk of gallstone disease by 3.4 times （P 〈 0.0001）. The prevalence of GSD in women who had 5 components of NetS was 5 times higher than in those without Nets component. The more the components of Mets, the higher the prevalence of GSD （P 〈 0.0001）. The presence of 5 components of the Mets increased the risk of gallstone disease by 4.0 times. CONCLUSION： GSD appears to be strongly associated with MetS, and the more the components of NetS, the higher the prevalence of GSD.

Technology for Enzymolysis of Jellyfish Brain Protein by Bromelain

[Objective] This study aimed to investigate the optimal technology for en- zymolysis of jeUyfish brain protein by bromefain. [Method] Effects of enzymolysis temperature, the amount of added enzyme, solid/liquid ratio, enzymolysis time and pH on the enzymolysis of total protein were investigated through single factor ex- periments, and some technical parameters were optimized by orthogonal experi- ments. [Result] The results revealed that enzymolysis temperature of 55℃, the amount of added enzyme of 4 000 U/g, solid/liquid ratio of 1：4, the amount of added enzyme time for 5 h and pH 7 were the optimal combination of parameters for the enzymolysis; the average degree of hydrolysis obtained from the verification tests was 36.9%, indicating that the optimized results of the orthogonal experiment were credible. [Conclusion] This study provides the optimized conditions reliable theo- retical foundation and technical support for the exploitation and utilization of jellyfish resources as medicines or functional food in China.