Objective： To investigate Leucine-rich repeat-containing G protein-coupled receptor 5 （LGR5） expressions in gastric cancer and to evaluate its clinical significance. Methods： LGR5 expression was assessed by immuno...Objective： To investigate Leucine-rich repeat-containing G protein-coupled receptor 5 （LGR5） expressions in gastric cancer and to evaluate its clinical significance. Methods： LGR5 expression was assessed by immunohistochemistry in 257 gastric cancer patients after surgery. The relationships between LGR5 expression and clinicopathological features and patients prognosis were statistically analyzed. Results： The expression of LGR5 was significantly higher in gastric cancers as a cancer stem cell marker than in adjacent normal tissues （P〈0.001）, and more frequently in patients with intestinal type, well-moderate differentiation and stage I and II （P〈0.05）. Although we found gastric cancer patients with LGR5 positive expression had a poorer prognosis, it didn＇t meet statistical significance （P〉0.05）. LGR5 negative expression was significantly related to the favorable overall survival in stage I and II gastric cancer patients （P〈0.05）. Furthermore, patients with high LGR5 expression tended to be more likely to get progression and have poorer progress-free survival （P〈0.05）. Multivariate Cox regression analysis revealed that LGR5 expression was an independent factor of overall survival for the patients with stage I and II gastric cancer （P〈0.05）. Conclusions： Our results show that LGR5 may play an important role in tumorigenesis and progression and would be a powerful marker to predict the prognosis of patients with stage I and II gastric cancer.展开更多
The slope instability and uneven settlement caused by the foundation pit dewatering pose a great threat to the project and surrounding environment.Thus,it is necessary to carry out optimization design of the foundatio...The slope instability and uneven settlement caused by the foundation pit dewatering pose a great threat to the project and surrounding environment.Thus,it is necessary to carry out optimization design of the foundation pit dewatering project.To solve this problem,this paper introduces the simulation-optimization method and establishes the groundwater model based on MODFLOW-2005.The local grid refinement(LGR)technique is employed to achieve the refinement of dewatering area.Under the premise of construction safety,the minimum cost of the project is set as the objective function to solve the optimization problem of foundation pit engineering with GWM-2005.The results show that the optimization with GWM-2005 will be more accurate combined with the LGR model,but the improvement of the optimization is not obvious.It is necessary to choose a suitable local refinement model considering the engineering requirement.展开更多
文摘Objective： To investigate Leucine-rich repeat-containing G protein-coupled receptor 5 （LGR5） expressions in gastric cancer and to evaluate its clinical significance. Methods： LGR5 expression was assessed by immunohistochemistry in 257 gastric cancer patients after surgery. The relationships between LGR5 expression and clinicopathological features and patients prognosis were statistically analyzed. Results： The expression of LGR5 was significantly higher in gastric cancers as a cancer stem cell marker than in adjacent normal tissues （P〈0.001）, and more frequently in patients with intestinal type, well-moderate differentiation and stage I and II （P〈0.05）. Although we found gastric cancer patients with LGR5 positive expression had a poorer prognosis, it didn＇t meet statistical significance （P〉0.05）. LGR5 negative expression was significantly related to the favorable overall survival in stage I and II gastric cancer patients （P〈0.05）. Furthermore, patients with high LGR5 expression tended to be more likely to get progression and have poorer progress-free survival （P〈0.05）. Multivariate Cox regression analysis revealed that LGR5 expression was an independent factor of overall survival for the patients with stage I and II gastric cancer （P〈0.05）. Conclusions： Our results show that LGR5 may play an important role in tumorigenesis and progression and would be a powerful marker to predict the prognosis of patients with stage I and II gastric cancer.
基金funded by Nanning Rail Transit Co., Ltd.the joint Foundation of Key Laboratory of Institute of Hydrogeology and Environmental Geology CAGS grant number (KF201611)
文摘The slope instability and uneven settlement caused by the foundation pit dewatering pose a great threat to the project and surrounding environment.Thus,it is necessary to carry out optimization design of the foundation pit dewatering project.To solve this problem,this paper introduces the simulation-optimization method and establishes the groundwater model based on MODFLOW-2005.The local grid refinement(LGR)technique is employed to achieve the refinement of dewatering area.Under the premise of construction safety,the minimum cost of the project is set as the objective function to solve the optimization problem of foundation pit engineering with GWM-2005.The results show that the optimization with GWM-2005 will be more accurate combined with the LGR model,but the improvement of the optimization is not obvious.It is necessary to choose a suitable local refinement model considering the engineering requirement.
