The alcohol and n-butanol extract of Potentilla anserine L. significantly protects myocardium from acute ischemic injury. However, its effects on rat hippocampal neurons and the mechanism of protection remain unclear....The alcohol and n-butanol extract of Potentilla anserine L. significantly protects myocardium from acute ischemic injury. However, its effects on rat hippocampal neurons and the mechanism of protection remain unclear. In this study, primary cultured hippocampal neurons from neonatal rats were incubated in 95% N2 and 5% CO2 for 4 hours. Results indicated that hypoxic injury decreased the viability of neurons, increased the expression levels of caspase-9 and caspase-3 mRNA, as well as cytochrome c, Caspase-9, and Caspase-3 protein. Pretreatment with 0.25, 0.062 5, 0.015 6 mg/mL n-butanol extract of Potentilla anserine L. led to a significant increase in cell viability. Expression levels of caspase-9 and caspase-3 mRNA, as well as cytochrome c, Caspase-9, and Caspase-3 protein, were attenuated. The neuroprotective effect of n-butanol extract of Potentilla anserine L. was equivalent to tanshinone IIA. Our data suggest that the n-butanol extract of Potentilla anserine L. could protect primary hippocampal neurons from hypoxic injury by deactivating mitochondrial cell death.展开更多
Potentilla anserina L.(PA)belongs to the Rosaceae family,is a common edible plant in the Qinghai-Tibet Plateau areas of China.This study elucidates the mechanism upon which crude polysaccharide of PA(PAP)on fat accumu...Potentilla anserina L.(PA)belongs to the Rosaceae family,is a common edible plant in the Qinghai-Tibet Plateau areas of China.This study elucidates the mechanism upon which crude polysaccharide of PA(PAP)on fat accumulation in HepG2 cells stimulated by oleic acid(OA)and high fat high sugar induced mice.The result revealed that PAP inhibited lipid accumulation in obese mice and ameliorated the degree of damage in OA-induced HepG2 cells.Specifically,compared to the control group,the TG and TC levels were decreased in cells and mice serum,the aspartate transaminase and alamine aminotransferase contents were declined in liver of obese mice by PAP treatment.The expressions of adipogenic genes of SREBP-1c,C/EBPα,PPARγ,and FAS were inhibited after PAP treatment.Moreover,PAP increased the mRNA levels of CPT-1 and PPARα,which were involved in fatty acid oxidation.The present results indicated the PAP could alleviate the damage of liver associated with obesity and PAP treatment might provide a dietary therapeutic option for the treatment of hyperlipidemia.展开更多
[Objectives]To study the effects of Fruticosa potentilla L.on the expression of key enzymes and hormones related to glucose and lipid metabolism in T2DM rats.[Methods]The ethyl acetate fraction of 95%methanol extract ...[Objectives]To study the effects of Fruticosa potentilla L.on the expression of key enzymes and hormones related to glucose and lipid metabolism in T2DM rats.[Methods]The ethyl acetate fraction of 95%methanol extract of F.potentilla(MEE)was administered orally to the T2DM rats,and the level of glucose and expression of insulin,resistin,leptin,glucokinase(GK)and glucose-6-phosphatase(G-6-Pase)in the rats were measured and detected.[Results]In the T2DM rats administered with MEE,the postprandial blood glucose level decreased,the expression levels of resistin,leptin and G-6-Pase were down-regulated,and the expression levels of insulin and GK were up-regulated(P<0.05,P<0.01).[Conclusions]F.potentilla can reduce effectively postprandial blood glucose level,regulate the expression of a variety of enzymes and hormones related to glucose and lipid metabolism and recover partially the insulin level in T2DM rats to achieve resistance to T2DM.展开更多
Contents of rutin and catechin in leaves and under-ground part of Potentilla fragarioides L.in spring and autumn were determined by HPLC technology.The results show that in spring,rutin content in leaves and under-gro...Contents of rutin and catechin in leaves and under-ground part of Potentilla fragarioides L.in spring and autumn were determined by HPLC technology.The results show that in spring,rutin content in leaves and under-ground part of P.fragarioides is 1.670 3 and 0.054 4 mg·g-1,and catechin content is 0.263 5 and 0.723 5 mg·g-1,respectively.In autumn,rutin content is 0.586 9 and 0.010 7 mg·g-1,and catechin content is 0.784 3 and 0.366 4 mg·g-1,respectively.The rutin content in leaves is the highest in spring,and catechin content in leaves is the highest in autumn.Therefore,collecting leaves in spring is suitable for taking rutin as a purpose,while collecting leaves in autumn is suitable for taking catechin as a purpose.展开更多
基金supported by the National Natural Science Foundation of China, No. 30672774 and No. 81073152the Great Program of Science Foundation of Tianjin, No.10JCZDJC21100
文摘The alcohol and n-butanol extract of Potentilla anserine L. significantly protects myocardium from acute ischemic injury. However, its effects on rat hippocampal neurons and the mechanism of protection remain unclear. In this study, primary cultured hippocampal neurons from neonatal rats were incubated in 95% N2 and 5% CO2 for 4 hours. Results indicated that hypoxic injury decreased the viability of neurons, increased the expression levels of caspase-9 and caspase-3 mRNA, as well as cytochrome c, Caspase-9, and Caspase-3 protein. Pretreatment with 0.25, 0.062 5, 0.015 6 mg/mL n-butanol extract of Potentilla anserine L. led to a significant increase in cell viability. Expression levels of caspase-9 and caspase-3 mRNA, as well as cytochrome c, Caspase-9, and Caspase-3 protein, were attenuated. The neuroprotective effect of n-butanol extract of Potentilla anserine L. was equivalent to tanshinone IIA. Our data suggest that the n-butanol extract of Potentilla anserine L. could protect primary hippocampal neurons from hypoxic injury by deactivating mitochondrial cell death.
基金supported by the Natural Science Foundation of Tibet Autonomous Region(XZ202201ZR0012G)Quality Evaluation and Efficient Utilization of Effective Components of Potentilla anserine Resources in Tibet(XZ202201ZD0001N).
文摘Potentilla anserina L.(PA)belongs to the Rosaceae family,is a common edible plant in the Qinghai-Tibet Plateau areas of China.This study elucidates the mechanism upon which crude polysaccharide of PA(PAP)on fat accumulation in HepG2 cells stimulated by oleic acid(OA)and high fat high sugar induced mice.The result revealed that PAP inhibited lipid accumulation in obese mice and ameliorated the degree of damage in OA-induced HepG2 cells.Specifically,compared to the control group,the TG and TC levels were decreased in cells and mice serum,the aspartate transaminase and alamine aminotransferase contents were declined in liver of obese mice by PAP treatment.The expressions of adipogenic genes of SREBP-1c,C/EBPα,PPARγ,and FAS were inhibited after PAP treatment.Moreover,PAP increased the mRNA levels of CPT-1 and PPARα,which were involved in fatty acid oxidation.The present results indicated the PAP could alleviate the damage of liver associated with obesity and PAP treatment might provide a dietary therapeutic option for the treatment of hyperlipidemia.
基金Supported by National Natural Science Foundation of China(No.81460652)Key Research and Development and Achievements Transformation Program of Qinghai Province(No.2018-SF-144)Project Funded by Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibet Plateau in Qinghai Province(No.2017-z-y25)
文摘[Objectives]To study the effects of Fruticosa potentilla L.on the expression of key enzymes and hormones related to glucose and lipid metabolism in T2DM rats.[Methods]The ethyl acetate fraction of 95%methanol extract of F.potentilla(MEE)was administered orally to the T2DM rats,and the level of glucose and expression of insulin,resistin,leptin,glucokinase(GK)and glucose-6-phosphatase(G-6-Pase)in the rats were measured and detected.[Results]In the T2DM rats administered with MEE,the postprandial blood glucose level decreased,the expression levels of resistin,leptin and G-6-Pase were down-regulated,and the expression levels of insulin and GK were up-regulated(P<0.05,P<0.01).[Conclusions]F.potentilla can reduce effectively postprandial blood glucose level,regulate the expression of a variety of enzymes and hormones related to glucose and lipid metabolism and recover partially the insulin level in T2DM rats to achieve resistance to T2DM.
文摘Contents of rutin and catechin in leaves and under-ground part of Potentilla fragarioides L.in spring and autumn were determined by HPLC technology.The results show that in spring,rutin content in leaves and under-ground part of P.fragarioides is 1.670 3 and 0.054 4 mg·g-1,and catechin content is 0.263 5 and 0.723 5 mg·g-1,respectively.In autumn,rutin content is 0.586 9 and 0.010 7 mg·g-1,and catechin content is 0.784 3 and 0.366 4 mg·g-1,respectively.The rutin content in leaves is the highest in spring,and catechin content in leaves is the highest in autumn.Therefore,collecting leaves in spring is suitable for taking rutin as a purpose,while collecting leaves in autumn is suitable for taking catechin as a purpose.
