With the progress of aging,the incidence of vascular calcification(VC)gradually increases,which is correlated with cardiovascular events and all-cause death,aggravating global clinical burden.Over the past several dec...With the progress of aging,the incidence of vascular calcification(VC)gradually increases,which is correlated with cardiovascular events and all-cause death,aggravating global clinical burden.Over the past several decades,accumulating approaches targeting the underlying pathogenesis of VC have provided some possibilities for the treatment of VC.Unfortunately,none of the current interventions have achieved clinical effectiveness on reversing or curing VC.The purpose of this review is to make a summary of novel perspectives on the interventions of VC and provide reference for clinical decision-making.展开更多
Objective: To investigate the inhibitory effects of combination chemotherapy of Carboplatin (CBP), Teniposide (Vm-26), Methasquin (MTX), and Nimodipine (NIM) on glioma, and to explore the sensitivity of gliom...Objective: To investigate the inhibitory effects of combination chemotherapy of Carboplatin (CBP), Teniposide (Vm-26), Methasquin (MTX), and Nimodipine (NIM) on glioma, and to explore the sensitivity of glioma cells to different treatment regimens so as to provide some clues for clinical usage of interstitial combination chemotherapy. Methods: MTT assay and 3H-TdR incorporation assay were performed to evaluate the inhibitory effects upon the proliferation of glioma cells, and to compare the sen- sitivity of glioma cells to administration of CBP, Vm-26, MTX, and NIM with that of the administration of CBP+NIM, Vm-26+NIM, MTX+NIM, CBP+Vm-26+MTX, or CBP+Vm-26+MTX+NIM, respectively. Results: The inhibition rate of CBP+Vm-26+MTX+NIM combination administration against glioma cells was 96.64%, higher than that of CBP+NIM (69.03%), Vm-26+NIM (71.53%), MTX+NIM (52.75%), CBP+Vm-26+MTX (78.59%) (P〈0.01), and the dosage of CBP, Vm-26, and MTX was declined to 1/10- 1/100 that of respective use of CBP, Vm-26, and MTX. Conclusion: The curative effect of combination administration of CBP, Vm-26, MTX, and NIM was much better than that of respective administration, suggesting a higher inhibition rate and a lower dosage use.展开更多
Objective: To explore the relationship between survivin and drug resistance, and the changes of the survivin expression in HL-60 cells treated with three kinds of chemotherapeutic drugs. Methods: HL- 60 cells were t...Objective: To explore the relationship between survivin and drug resistance, and the changes of the survivin expression in HL-60 cells treated with three kinds of chemotherapeutic drugs. Methods: HL- 60 cells were treated with appropriate concentration of daunomycin (DNR), mitoxantrone (MIT) or arsenic trioxide (As2O3). The expression of survivin mRNA and protein on the first or third day was detected by RT-PCR and Western blot respectively. Results: The expression of survivin mRNA was decreased on the first day by 10% in DNR-treated group, 40% in MIT-treated group (P〈0.01) and 25% in As2O3-treated group (P〈0.01) respectively. On the third day, the expression of survivin mRNA in DNR- and MIT-treated group was up-regulated to 120% (P〈0.05) and 165% (P〈0.01) respectively as compared with that on the first day, but down-regulated to 68% in As2O3-treated group (P〈0.01). As compared with control group, the expression of survivin protein in DNR- or MIT-treated group was increased by 14% or 11% on the third day respectively, but it was decreased by 18% in As2O3-treated group. Conclusion: In DNR- and MIT-treated group, the expression of surivin was decreased at first and then increased obviously, which may be one of the causes for resistance to chemotherapy against leukemia. Different from other two drugs, As2O3 may play an important role in restoring chemotherapy sensitivity.展开更多
Paraoxonase-1 (PON1) is an esterase and lactonase synthesized by the liver and found in the circulation associated with high-density lipoproteins. The physiological function of PON1 seems to be to degrade specific oxi...Paraoxonase-1 (PON1) is an esterase and lactonase synthesized by the liver and found in the circulation associated with high-density lipoproteins. The physiological function of PON1 seems to be to degrade specific oxidized cholesteryl esters and oxidized phospholipids in lipoproteins and cell membranes. PON1 is, therefore, an antioxidant enzyme. Alterations in circulating PON1 levels have been reported in a variety of diseases involving oxidative stress including chronic liver diseases. Measurement of serum PON1 activity has been proposed as a potential test for the evaluation of liver function. However, this measurement is still restricted to research and has not been extensively applied in routine clinical chemistry laboratories. The reason for this restriction is due to the problem that the substrate commonly used for PON1 measurement, paraoxon, is toxic and unstable. The recent development of new assays with non-toxic substrates makes this proposal closer to a practical development. The present editorial summarizes PON1 biochemistry and function, its involvement with chronic liver impairment, and some aspects related to the measurement of PON1 activity in circulation.展开更多
基金supported by the Peking University Baidu Fund (2019BD019)
文摘With the progress of aging,the incidence of vascular calcification(VC)gradually increases,which is correlated with cardiovascular events and all-cause death,aggravating global clinical burden.Over the past several decades,accumulating approaches targeting the underlying pathogenesis of VC have provided some possibilities for the treatment of VC.Unfortunately,none of the current interventions have achieved clinical effectiveness on reversing or curing VC.The purpose of this review is to make a summary of novel perspectives on the interventions of VC and provide reference for clinical decision-making.
文摘Objective: To investigate the inhibitory effects of combination chemotherapy of Carboplatin (CBP), Teniposide (Vm-26), Methasquin (MTX), and Nimodipine (NIM) on glioma, and to explore the sensitivity of glioma cells to different treatment regimens so as to provide some clues for clinical usage of interstitial combination chemotherapy. Methods: MTT assay and 3H-TdR incorporation assay were performed to evaluate the inhibitory effects upon the proliferation of glioma cells, and to compare the sen- sitivity of glioma cells to administration of CBP, Vm-26, MTX, and NIM with that of the administration of CBP+NIM, Vm-26+NIM, MTX+NIM, CBP+Vm-26+MTX, or CBP+Vm-26+MTX+NIM, respectively. Results: The inhibition rate of CBP+Vm-26+MTX+NIM combination administration against glioma cells was 96.64%, higher than that of CBP+NIM (69.03%), Vm-26+NIM (71.53%), MTX+NIM (52.75%), CBP+Vm-26+MTX (78.59%) (P〈0.01), and the dosage of CBP, Vm-26, and MTX was declined to 1/10- 1/100 that of respective use of CBP, Vm-26, and MTX. Conclusion: The curative effect of combination administration of CBP, Vm-26, MTX, and NIM was much better than that of respective administration, suggesting a higher inhibition rate and a lower dosage use.
基金This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30370595).
文摘Objective: To explore the relationship between survivin and drug resistance, and the changes of the survivin expression in HL-60 cells treated with three kinds of chemotherapeutic drugs. Methods: HL- 60 cells were treated with appropriate concentration of daunomycin (DNR), mitoxantrone (MIT) or arsenic trioxide (As2O3). The expression of survivin mRNA and protein on the first or third day was detected by RT-PCR and Western blot respectively. Results: The expression of survivin mRNA was decreased on the first day by 10% in DNR-treated group, 40% in MIT-treated group (P〈0.01) and 25% in As2O3-treated group (P〈0.01) respectively. On the third day, the expression of survivin mRNA in DNR- and MIT-treated group was up-regulated to 120% (P〈0.05) and 165% (P〈0.01) respectively as compared with that on the first day, but down-regulated to 68% in As2O3-treated group (P〈0.01). As compared with control group, the expression of survivin protein in DNR- or MIT-treated group was increased by 14% or 11% on the third day respectively, but it was decreased by 18% in As2O3-treated group. Conclusion: In DNR- and MIT-treated group, the expression of surivin was decreased at first and then increased obviously, which may be one of the causes for resistance to chemotherapy against leukemia. Different from other two drugs, As2O3 may play an important role in restoring chemotherapy sensitivity.
基金Supported by Fondo de Investigación Sanitaria,FIS 00/0232,02/0430, 05/1607the Instituto de Salud Carlos Ⅲ, C03/02,C03/08,G03/015the Generalitat de Catalunya,FI 05/00068
文摘Paraoxonase-1 (PON1) is an esterase and lactonase synthesized by the liver and found in the circulation associated with high-density lipoproteins. The physiological function of PON1 seems to be to degrade specific oxidized cholesteryl esters and oxidized phospholipids in lipoproteins and cell membranes. PON1 is, therefore, an antioxidant enzyme. Alterations in circulating PON1 levels have been reported in a variety of diseases involving oxidative stress including chronic liver diseases. Measurement of serum PON1 activity has been proposed as a potential test for the evaluation of liver function. However, this measurement is still restricted to research and has not been extensively applied in routine clinical chemistry laboratories. The reason for this restriction is due to the problem that the substrate commonly used for PON1 measurement, paraoxon, is toxic and unstable. The recent development of new assays with non-toxic substrates makes this proposal closer to a practical development. The present editorial summarizes PON1 biochemistry and function, its involvement with chronic liver impairment, and some aspects related to the measurement of PON1 activity in circulation.