Documentation Concordance,Sharing and Utilization of Tea Germplasm Resources in Yunnan

In this paper,the research achievements and progress of Yunnan tea germplasm resource in past sixty years are systematically reviewed from the following aspects：exploration,collecting,conservation,protection,identification,evaluation and shared utilization.Simultaneously,the current problems and the suggestions about subsequent development of tea germplasm resources in Yunnan were discussed,including superior and rare germplasm collection,tea genetic diversity research,biotechnology utilization in tea germplasm innovation,super gene exploration and function,the construction of utilization platform,biological base of species and population conservation.

Difference Analysis on Contents of Gallic Acid and Catechins of Tea Resources from Yunnan Province

[Objective] This study aimed to analyze the difference in the contents of gallic acid and catechins of tea resources from Yunnan Province. [Method] By using high performance liquid chromatography （HPLC）, the contents of gallic acid （GA）, catechins （C）, epicatechin （EC）, epigallocatechin （EGC）, epicatechin gallate （ECG） and epigallocatechin gallate （EGCG） of 121 tea germplasms from the China National Germplasm Tea Repositories （CNGTR） at the Tea Research Institute of Yunnan Academy of Agricultural Sciences （TRIYAAS） were measured. [Result] The content of GA ranged from 0.210% to 1.902%, with an average of 0.834%, explaining rela- tively low GA content among tea germplasms. The content of C ranged from 0.069% to 8.865%, with an average of 1.916%. The content of EC ranged from 0.126% to 2.865%, with an average of 1.112%. The content of EGC ranged from 0.00% to 3.709%, with an average of 0.954%. The content of ECG ranged from 0.739% to 8.957%, with an average of 4.063%. The content of EGCG ranged from 0.819% to 11.77%, with an average of 5.939%. The content of total C ranged from 6.354% to 22.654%, with an average of 14.042%. [Conclusion] There was relatively big difference of catechin contents among different tea resources, indicating that there was plentiful biodiversity of Yunnan tea germplasms. At the same time, three tea germplasms with high epigallocatechin gallate content （≥10%） was selected preliminarily, which would provide important materials for breeding tea cultivars with high EGCG content in the future.

Effects of Climatic Factors on Tree-ring Maximum Latewood Density of Picea schrenkiana in Xinjiang, China

Based on two tree-ring maximum latewood density （MXD） chronologies of Picea schrenkiana from the Manas River Basin, Xinjiang, the response characteristics of MXD to climate variation was discussed. Correlation analysis between MXD chronologies and instrumental records from Shihezi meteorological station showed that each chronology was significantly and positively correlated with the maximum monthly average temperature in July-August, and especially, the regional chronology （RC） was the most highly correlated variable （r=0.54, P〈0.001）. Afterwards, the maximum average temperature in July-August was reconstructed using RC. Comparison among reconstructed temperature, observed values, and the drought index （Is） confirmed that precipitation would affect MXD when the absolute value of Is is greater than 1.5σ （｜Is｜ 〉 2.5 during 1953-2008） or near to 1.5a over a 2-3 year period. The response characteristics are related to the semiarid climate of the study area. In dry years, lack of precipitation would limit the thickening of latewood cell walls and, as a result, impact MXD. Therefore, compared with relatively humid regions, the response of tree-ring MXD to air temperature similarly would be influenced by extreme moisture conditions in semiarid areas, and MXD, as a temperature proxy, should be used prudently on a limited scale.

Mineral Elements in Different Varieties of Camellia sinensis

To understand the mineral elements in different varieties of tea （ Camellia sinensis）, 17 mineral elements in eight tea varieties including Yunnan Dayezhong, No. 43 Longjing and No. 6 Zaobaijian, were measured. The results showed that nine elements, such as P, K, Ba, Mn, Cu, were significantly different among varieties, others did not. Black tea varieties usually contain high contents of Cu and K. As a conclusion, mineral elements should be extensively considered in breeding tea varieties.

Walnut Tree Grafting Experiments in Different Areas in Yunnan Province

Yunnan has an area of 2.88 million hm2 walnut trees. Due to freezing in- jury and rapid growth, however, there are a lot of iron walnuts or inferior-quality walnuts ready for grafting. In five years, we conducted experiments in Kunming （Yunnan Academy of Forestry）, Gongshan County, Gucheng City, Luliang County, and Ludian County by cleft grafting, veneer grafting, bark grafting and whip grafting. The grafting approaches should be selected according to grafting requirements, characteristics, and survival rate. For example, with a low survival rate, cleft grafting can be abandoned, and the rest approaches might be chosen as per practical demands.

Breeding and Characterization of Yunchachunhao, a New Tea Variety with Full Aroma and High Quality

[Objective] The aim of this study was to select a tea variety with full aro- ma and high quality. [Method] The quality of Yunchachunhao was identified using Yunkang 10 and Fudingdabaicha as the controls in variety comparison test, using Yunkang 10 and Dangdidayezhong as the controls in reginal test. [Result] In variety comparison test, the yield of Yunchachunhao was 39.3% more than that of Yunkang 10, and 29.6% more than that of Fudingdabaicha. In reginal test, the yield of Yun- chachunhao was 2.5% （Baoshan） and 10.2% （Lancang） more than that of Yunkang 10. The roast-dried green tea of Yunchachunhao was characterized by flowery fla- vor, good taste and bright green infused leaves. It got the total scores of 91.13 in quality identification, which was higher than that of Fudingdabaicha （90.75） and Yunkang 10 （88.85）. The steam fixed green tea of Yunchachunhao contained 28.0% polyphenols and 2.7% amino acids; and the ratio of polyphenols to amino acids was 10.4, lower than that of Fudingdabaicha （10.6） and Yunkang 10 （17.3）. Yun- chachunhao had better resistance to drought and blister blight than Yunkang 10. [Conclusion] The survival rates of cuttings and transplanted seedlings of Yun- chachunhao are higher than that of control varieties, suggesting it has better adapt- ability and can be promoted in the large-leaf tea planting area of Yunnan Province.

真实与虚幻

很久以前,曾经做过一个梦:微风在沙漠上行走,天地间是一片柔黄的色调,几株纤细的枯枝伸向天空,背衬远天几缕洁白的流云,宛若玉树临风。

Temperature dependence of nitrogen mineralization and microbial status in O_H horizon of a temperate forest ecosystem

It was hypothesized that increasing air and/or soil temperature would increase rates of microbial processes including litter decomposition and net N mineralization, resulting in greater sequestration of carbon and nitrogen in humus, and consequently development in OH horizon （humus horizon）. To quantify the effect of temperature on biochemical processes controlling the rate of OH layer development three adjacent forest floors under beech, Norway spruce and mixed species stands were investigated at Soiling forest, Germany by an incubation experiment of OH layer for three months. Comparing the fitted curves for temperature sensitivity of OH layers in relation to net N mineralization revealed positive correlation across all sites. For the whole data set of all stands, a Q10 （temperature sensitivity index） value of 2.35-2.44 dependent on the measured units was found to be adequate for describing the temperature dependency of net N mineralization at experimental site. Species-specific differences of substrate quality did not result in changes in biochemical properties of OH horizon of the forest floors. Temperature elevation increased net N mineralization without significant changes in microbial status in the range of I to 15℃. A low Cmic /Corg （microbial carbon/organic carbon） ratio at 20℃ indicated that the resource availability for decomposers has been restricted as reflected in significant decrease of microbial biomass.

Ecological Species Groups and Interspecific Association of Dominant Tree Species in Daiyun Mountain National Nature Reserve

Abstract： Research on the ecological species groups and interspecific association of plant species are helpful to discover species coexistence processes and mechanisms, and to more fully understand plant community structure, function, and its taxonomy. However, little is known about the ecological species groups （ESG） and the interspecific association of dominant species in Daiyun Mountain National Nature Reserve of Fujian Province, China. Therefore, the main goal of this paper is to explore the ESG using maximal tree, and to analyze interspecific associations of 32 dominant species selected from lo2 sample plots using the chi-square test. The results show that： （1） 32 dominant species have a significant overall positive interspecific association, which indicates that the natural forest in Daiyun Mountain National Nature Reserve is stable, （a） The species pairs with weak associations, non-associations and positive associations account for lo.88%, 29.64% and 59.48% of the total 496 species pairs respectively, which suggests that the population distributions of the dominant species investigated are relatively independent, （3） The following species pairs may be useful for practical application, 〈Pinus taiwanensis, Rhododendron farrerae〉, 〈Castanopsis carlesii, Altingia chinensis〉, 〈C. carlesii, Castanopsis fargesii〉, 〈Castanopsis eyrei, C. fargesii〉, 〈P. taiwanensis, Fagus lucida〉 , 〈Machilus thunbergii, Castanopsis nigrescens〉, and （4） The results of clustering analysis based on the maximal tree method indicates that the 32 dominant species can be divided into 3 ESGs when A at o.6o, that is ESG I {Pinus massoniana, Cunning hamia lanceolata}, ESG II {P. taiwanensis, R. farrerae, Enkianthus quinqueflorus}, ESG III {C. carlesii, A. chinensis, C. eyrei, Castanopsis fabri, C. fargesii, Schima superba, Machilus thunbergii, Rhododendron latoueheae}. The results may be used for the selection of afforestation tree species in South China Forest Areas and guide the natural management of plantations.

Growth Analysis of Nyssa yunnanensis Saplings

The growth of Nyssa yunnanensis saplings in Guanping area was monitored in 2011, 2013 and 2015. The results showed that the average basal diameter growth rate during 2013-2015 was higher than that during 2011-2013, and that during 2009-2011 was lowest; the average height growth rates in the durations of 2011-2013 and 2013-2015 were basically the same, and that during 2009-2011 was relatively slow; the growth of diameter at breast height（DBH） in the duration of 2013-2015 was significantly higher than that in the duration of 2011-2013. Comprehensive and better nature-returning work, as well as long-term and stable funding, is required for the protection of Nyssa yunnanensis.

Wet Canopy Evaporation Rate of Three Stands in Western Sichuan, China

The wet canopy evaporation rate (Er) wascalculated by Penman-Monteith combination model based on three assumptions and with meteorological variables 2 m above the canopy inthree stands, dominated by spruce (SF), fir (FF) and birch (BF) trees, respectively[(,)( )]in the subalpineforests in western Sichuan, China over a growingseason. The total amount of the E was 44.5 mm forSF, 88.5 mm for FF and 57.8 mm for BF, accounting for 9.2%, 16.6% and 10.2% of the gross rainfall,respectively, in the measuring period. There was the highest average monthly Er and percentage of E togross rainfall for FF compared with SF and BF.Mean Er was 0.097 mm h-1 (ranging from 0.028 to0.487 mm h-1), 0.242 mm h-1 (from 0.068 to 0.711 mm h-1) and 0.149 mm h-1 (from 0.060 to 0.576 mm h-1for SF, FF and BF, respectively. The highest average monthly Er occurred in June was 0.120 mm h-1 forSF, 0.317 mm h-1 for FF and 0.169 mm h-1 for BF, and the lowest value in October was 0.083 mm h-1 for SF, 0.187 mm h-1 for FF and 0.101 mm h-1 for BF,respectively. The averages of Er from 8:00 to 16:00were significantly higher than those from 0:00 to8:00 and from 16:00 to 0:00 for the three stands. The marked daily and monthly differences of Er were contributable to the variations of solar radiation, air temperature and relative humidity above thecanopy.

Beneficial effects of cold-moist stratification on seed germination be- haviors of Abies pindrow and Picea smithiana

A study was conducted to evaluate the effect of GA3, moist-chilling and temperature on seed germination ofAbies pindrow and Picea smithiana from five different provenances. Seeds were soaked in GA3 （10 mg·L^-1） for 24 h, then chilled at 3-5℃ for 15 days. Four temperature regimes viz. 10℃, 15℃, 20℃ and 25℃ were used for stimulating seed germination. Results showed that soaking and chilling significantly increased germination percentage. The germination percentage was highest at 10℃. Overall results showed that soaking seeds in GA3 （10 mg·L^-1） for 24 h, moist chilling for 15 days, and germinating at 10℃ produced an effective germination in both the species studied. The statistical analysis of the data proclaimed significant effect of treatment, temperature, provenance and treatment with temperature interactions on seed germination.

Plant biotechnology:a case study of Bacillus thuringiensis(Bt) and its application to the future of genetic engineered trees

Agricultural productivity may be raised in a sustainable way by many different technologies such as biological fertilizers, soil and water conservation, biodiversity conservation, improved pest control, and changes in land ownership and distribution. Of these measures, biotechnology applications probably hold the most promise in augmenting conventional agricultural productivity, because biotechnology applications give not only the need to increase production, but also protect the environment and conserving natural resources for future generations. Biotechnology applications will have the possibilities to increase productivity and food availability through better agronomic performance of new varieties, including resistance to pests; rapid multiplication of disease-free plants; ability to obtain natural plant products using tissue culture; diagnosis of diseases of plants and livestock; manipulation of reproduction methods increasing the efficiency of breeding; and the provision of incentives for greater participation by the private sector through investments. Insect resistance through the transfer of a gene for resistance fromBacillus thuringiensis (Bt) is one of the most advanced biotechnology applications already being commercialized in many parts of the world. This paper reviews the development and the status ofBt technology and application ofBt transgenic plants in current agriculture, and discusses specific issues related to the transfer of the technology to the future of genetic engineered trees with emphasis on conifers. Key words Agricultural productivity - Bacillus thuringiensis - Genetic engineering - Insect resistance - Trees CLC number Q812 - S763.306 Document code A Biography: Tang Wei (1964-), male, Ph. Doctor, Research associate, Department of Biology, Howell Science Complex, East Carelina University, Greenville, NC 27858-4353, USA.Responsible editor: Chal Ruihai

Influence of Human Pressure on Forest Resources and Productivity at Stand and Tree Scales: The Case Study of Yunnan Pine in SW China

This paper examines human impact on stands and individual trees of Pinus yunnanensis growing near the small mountain villages of Pianshui and Yangjuan in southwestern Sichuan Province,China.In an effort to assess whether use of these forests was sustainable,we examined the effects of human use in two ways.First,we directly measured the effect of cutting branches,for fuel and fodder,on tree growth.We hypothesized that branch cutting would negatively impact tree growth.We established 12 plots on four hills and compared 14 pairs of trees,one tree in each pair with an apparently full crown and the other with a considerable portion of the crown removed.Second,we assessed stand and tree properties over a 500 m elevation gradient above the villages where we hypothesized that as elevation increases,stand and tree properties should show fewer human impacts.Although extensive branch cutting reduced the live crown,tree height and diameter,compensatory processes likely enabled trees to recover and to add basal area increments(BAIs) similar to those added by trees with full crowns.Trees and stands close to villages showed less growth and lower basal areas,respectively,than stands and trees at intermediate or distant elevations from villages.Areas relatively close to the villages showed considerable effects of human-related disturbances such as branch cutting,grazing,tree and shrub removal,losses of litter,and human and animal trails.Such areas had increased soil erosion and often loss of the ‘A' horizon.Stands close to villages had younger trees,lower stand basal areas,smaller basal area increments,and more stumps.Our results suggest an increasingly vulnerable interface between occupants of these two villages and their surrounding forests.

Effects of climate change on potential habitats of the cold temperate coniferous forest in Yunnan province, southwestern China

We built a classification tree （CT） model to estimate climatic factors controlling the cold temperate coniferous forest （CTCF） distributions in Yunnan province and to predict its potential habitats under the current and future climates, using seven climate change scenarios, projected over the years of 2070-2099. The accurate CT model on CTCFs showed that minimum temperature of coldest month （TMW） was the overwhelmingly potent factor among the six climate variables. The areas of TMW〈-4.05 were suitable habitats of CTCF, and the areas of -1.35 〈 TMW were non-habitats, where temperate conifer and broad-leaved mixed forests （TCBLFs） were distribute in lower elevation, bordering on the CTCF. Dominant species of Abies, Picea, and Larix in the CTCFs, are more tolerant to winter coldness than Tsuga and broad-leaved trees including deciduous broad-leaved Acer and Betula, evergreen broad- leaved Cyclobalanopsis and Lithocarpus in TCBLFs. Winter coldness may actually limit the cool-side distributions of TCBLFs in the areas between -1.35℃ and -4.05℃, and the warm-side distributions of CTCFs may be controlled by competition to the species of TCBLFs. Under future climate scenarios, the vulnerable area, where current potential （suitable ＋ marginal） habitats （80,749 km^2） shift to non-habitats, was predicted to decrease to 55.91% （45,053 km^2） of the current area. Inferring from the current vegetation distribution pattern, TCBLFs will replace declining CTCFs. Vulnerable areas predicted by models are important in determining priority of ecosystem conservation.

Stem flow chemistry of Picea glehnii,Abies sachalinensis and Alnus japonica and its effect on the peat pore water chemistry in an ombrogenous mire in Ochiishi,eastern Hokkaido,Japan

We investigated the chemical properties of stemflow of Picea glehnii,Abies sachalinensis and Alnus japonica as well as peat pore water chemistry,including the distance and depth profiles of pore water chemistry,in an ombrogenous mire.The effect of stemflow on the peat pore water chemistry was clear at the stem base in the peat forest in the mire,and the peat pore water around the stem base of a tree had its own chemical properties specific to each species.P.glehnii showed the highest concentration of salts both in stemflow and peat-pore water,whereas A.japonica showed the lowest concentrations;however,the gradient of the chemical environment from the stem base to outside of the canopy is formed.The peat pore water chemistry under the canopy was mainly controlled by the chemical processes diluted by the abundant peat pore water;the stemflow movement in the high water content of the peat was more slowly because of the flat topography（〈 1o）.This would be due to the fact that the chemicals in stemflow would be diluted by the abundant peat pore water.The spatial heterogeneity of chemical environment between microsites within forested peatland would be also contributed indirectly through the control of microorganism activity,and nutrient regeneration mediated the surface water and the stemflow of the dominant canopy trees.

Oldest Peach Fossil Found in Yunnan

Prunus persica（peach）is an economically important deciduous tree in the Rosaceae family that produces20 million tons of fruit per year.China has a long history of peach cultivation known from both historical and archaeological evidences.So far.

NDVI Variation of Tree Rings between March and May since 1915 in the Eastern Part of the Qilian Mountains of China

[Objective] The aim was to analyze the correlation between tree-ring width of Picea crassifolia in the east of Qilian Mountains and the precipitation, temperature and the normalized difference vegetation index (NDVI). [Method] The correlation analysis and the regression analysis were used in this study. [Result] The tree-ring width was significantly correlated with the autumn precipitation and the spring average NDVI. The conversion equation between tree-ring width and spring NDVI (R2 = 48.5% , R2adj =46.2% , F =21.627, P <0.001) was developed and NDVI sequence was reconstructed during the period 1915 -2007. The drought in the 1920s was pronounced. Vegetation cover in the Qilian Mountains increased during the period 1922-1934, 1940-1957, 1965-1969, 1984-1988 and 1995-1997, but decreased during 1935 -1939, 1958 -1964, 1970 -1983, 1989 -1994 and 1998 -2005. [Conclusion] The reconstructed NDVI showed the drought evolution in the study area.