In order to evaluate the immune effect of the protein expressed by the universal vector pET-mLTA-CTLA-4 plus IBDV subunit, the fusion protein mLTA-CTLA-4 was expressed and purified. Protein toxicity tests were carried...In order to evaluate the immune effect of the protein expressed by the universal vector pET-mLTA-CTLA-4 plus IBDV subunit, the fusion protein mLTA-CTLA-4 was expressed and purified. Protein toxicity tests were carried out on rabbits.The VP2 gene of infectious bursal virus was amplified by RT-PCR, and lately used for pET-VP2 construction. Ten-day-old free healthy chickens were chosen for a grouped test, including the mLTA-CTLA-4（at different doses） plus VP2 groups, IBDV living vaccine group and control group. Serum and mucosal samples were collected regularly and the neutralization titers of IgG and IgA were assayed, while an animal protection test was conducted to determine the protection rate. The results showed that the protein m LTA-CTLA-4 was non-toxic and its protection rate was100%. IgG or IgA levels in the IBDV vaccine group were slightly higher than those in recombinant protein groups. These results indicated that the recombinant protein mLTA-CTLA-4 could be applied with IBDV subunit vaccine to protect chickens from infection.展开更多
The purpose of our paper is to show, on the example of Tbilisi, how places become sacred and the sights of pilgrimage; what are the modern and traditional forms of pilgrimage in Georgia. The study showed that the grav...The purpose of our paper is to show, on the example of Tbilisi, how places become sacred and the sights of pilgrimage; what are the modern and traditional forms of pilgrimage in Georgia. The study showed that the graves of the prominent people for their pure life and miraculous deeds, who lived during the Soviet and post-Soviet periods until now are the places of veneration. These people before official canonization became saints for people and as I call them, they became “folk Saints”, as the forms of their veneration are the same as canonized ones. After post-Soviet revival of religiousness in the late 1970es, one form of pilgrimage became especially popular in the Orthodox Church of Georgia. On the memorial days of Saints or just after Sunday liturgy priest and congregation visit graves of these Saints or prominent churches dedicated to them, where they pray and have small services. The study showed that this form was not traditional Georgian form of pilgrimage, but Russian one. I also argue that there might have been five forms of traditional pilgrimage in Georgia. The study was based on historical sources, published scientific works and the personal field ethnographic materials.展开更多
Advances of studies on the acupuncture and pain signal transduction mechanisms in complete Freud＇s adjuvant arthritis are reviewed from the three aspects, the first messenger of modulating pain signals and the relate...Advances of studies on the acupuncture and pain signal transduction mechanisms in complete Freud＇s adjuvant arthritis are reviewed from the three aspects, the first messenger of modulating pain signals and the related receptors, the second messenger of modulating pain signals and other factors possibly involved in modulation of pain signal transduction, etc. It is held that modulation of acupuncture for pain signals is a comprehensive course involved in multi-channels, multi-levels, multi-links, and in future, acupuncture analgesic mechanisms for Freud＇s adjuvant arthritis will be more deeply studied by use of more new techniques and new methods.展开更多
基金Supported by Jiangsu Provincial Postdoctoral Science Foundation(1401077B)Open Project of Jiangsu Provincial Key Laboratory of Veterinary Bio-pharmaceutical High-tech Research(JSKLKF1403)+3 种基金the Fenghuang Talent Engineering Project of Jiangsu Agrianimal Husbandry Vocational CollegeKey Project of Jiangsu Agri-animal Husbandry Vocational College(NSFZD1405)the Horizontal Cooperation Project of Yangzhou Chaotiange Agri-animal Husbandry Science and Technology Co.,Ltd.(00010114012,NSFPT201510)the Special Fund for Jiangsu Huaneng Medical Investment Co.,Ltd.(NSFPT201512)~~
文摘In order to evaluate the immune effect of the protein expressed by the universal vector pET-mLTA-CTLA-4 plus IBDV subunit, the fusion protein mLTA-CTLA-4 was expressed and purified. Protein toxicity tests were carried out on rabbits.The VP2 gene of infectious bursal virus was amplified by RT-PCR, and lately used for pET-VP2 construction. Ten-day-old free healthy chickens were chosen for a grouped test, including the mLTA-CTLA-4（at different doses） plus VP2 groups, IBDV living vaccine group and control group. Serum and mucosal samples were collected regularly and the neutralization titers of IgG and IgA were assayed, while an animal protection test was conducted to determine the protection rate. The results showed that the protein m LTA-CTLA-4 was non-toxic and its protection rate was100%. IgG or IgA levels in the IBDV vaccine group were slightly higher than those in recombinant protein groups. These results indicated that the recombinant protein mLTA-CTLA-4 could be applied with IBDV subunit vaccine to protect chickens from infection.
文摘The purpose of our paper is to show, on the example of Tbilisi, how places become sacred and the sights of pilgrimage; what are the modern and traditional forms of pilgrimage in Georgia. The study showed that the graves of the prominent people for their pure life and miraculous deeds, who lived during the Soviet and post-Soviet periods until now are the places of veneration. These people before official canonization became saints for people and as I call them, they became “folk Saints”, as the forms of their veneration are the same as canonized ones. After post-Soviet revival of religiousness in the late 1970es, one form of pilgrimage became especially popular in the Orthodox Church of Georgia. On the memorial days of Saints or just after Sunday liturgy priest and congregation visit graves of these Saints or prominent churches dedicated to them, where they pray and have small services. The study showed that this form was not traditional Georgian form of pilgrimage, but Russian one. I also argue that there might have been five forms of traditional pilgrimage in Georgia. The study was based on historical sources, published scientific works and the personal field ethnographic materials.
基金Supported by Scientific Research Project Foundation of Shanghai City Science and Technology Committee:07dz19722-5
文摘Advances of studies on the acupuncture and pain signal transduction mechanisms in complete Freud＇s adjuvant arthritis are reviewed from the three aspects, the first messenger of modulating pain signals and the related receptors, the second messenger of modulating pain signals and other factors possibly involved in modulation of pain signal transduction, etc. It is held that modulation of acupuncture for pain signals is a comprehensive course involved in multi-channels, multi-levels, multi-links, and in future, acupuncture analgesic mechanisms for Freud＇s adjuvant arthritis will be more deeply studied by use of more new techniques and new methods.
