AIM- To immunohistochemicaUy examine micrometastasis and VEGF-C expression in hilar bile duct carcinoma （HBDC） and to evaluate the clinical significance of the results. METHODS： A total of 361 regional lymph nodes ...AIM- To immunohistochemicaUy examine micrometastasis and VEGF-C expression in hilar bile duct carcinoma （HBDC） and to evaluate the clinical significance of the results. METHODS： A total of 361 regional lymph nodes from 25 patients with node-negative HBDC were immunostained with an antibody against cytokeratins 8 and 18 （CAM 5.2）, and immunohistochemical staining of VEGF-C was performed in 34 primary resected tumors. RESULTS： Lymph node micrometastasis was detected in 6 （24%） of the 25 patients and 10 （2.8%） of the 361 lymph nodes. Patients with micrometastasis showed significantly poorer survival rates than those without （P= 0.025）. VEGF-C expression was positive in 17 （50%） of 34 HBDC, and significantly correlated with lymph node metastasis （P=0.042） and microscopic venous invasion （P=0.035）. CONCLUSIONS： It is suggested that immunohistochemically detected lymph node micrometastasis has an impact on the outcome of HBDC. VEGF-C expression is highly correlated with lymph node metastasis in HBDC and might therefore be a useful predictor.展开更多
Objective: To observe the clinical therapeutic effect of acupuncture plus intensive pulse light irradiation on chloasma. Methods: Ninety-six cases of chloasma were randomly divided into two groups, the control group o...Objective: To observe the clinical therapeutic effect of acupuncture plus intensive pulse light irradiation on chloasma. Methods: Ninety-six cases of chloasma were randomly divided into two groups, the control group of 46 cases treated by simple acupuncture and the treatment group of 50 cases treated by acupuncture and intensive pulse light irradiation. Results: The total effective rate was 89.1% and 98.0% in the control group and treatment group respectively, with a significant difference between the two groups (P<0.05). Conclusion: For chloasma, the effect of treatment with acupuncture plus intensive pulse light irradiation is superior to that with simple acupuncture.展开更多
Point selection along the course of meridians is the basic principle for acupuncture prescription and selection of points. In application, there are three categories of point selection: selection of local and adjacent...Point selection along the course of meridians is the basic principle for acupuncture prescription and selection of points. In application, there are three categories of point selection: selection of local and adjacent points; selection of distant points, and selection of symptomatic points. Based on the展开更多
Objective To investigate the clinical efficacy of electroacupuncture for the treatment of Achilles tendonitis. Methods Sixty patients with Achilles tendonitis at the age of 18-55 years old were randomly divided into e...Objective To investigate the clinical efficacy of electroacupuncture for the treatment of Achilles tendonitis. Methods Sixty patients with Achilles tendonitis at the age of 18-55 years old were randomly divided into electroacupuncture group （group A） and low frequency impulse group （group B） according to the random number table with 30 patients in each group. Chengshan （承山 BL 57）, Ashi point, Taixi （太溪 KI 3） and Kunlun （昆仑 BL 60） were selected as main acupoints, local point selection was adopted as main method and distal point selection as supplement. Patients in group A were treated with electroacupuncture and in group B were treated with low frequency impulse therapeutic apparatus. Patients in both groups were treated for 12 times, 3 times per week in the 1st and 2nd weeks, twice a week in the 3rd and 4th weeks, and once a week in the Sth and 6th weeks, with a total of 6 weeks. The heel pain VAS scores, tenderness and efficacy of dysfunction in two groups were comparatively analyzed. Results Pain： there was significant difference in pain scores in group A before and after treatment （1.67±0.71 vs 3.03±1.81, P〈0.01）, and the difference in pain scores was not significant in group B before and after treatment （2.13±1.17 vs 2.87±1.57, P〉0.05）. Tenderness： In group A, 13 patients had mild tenderness, 15 had moderate tenderness and 2 had severe tenderness before treatment. After treatment, 26 patients had mild tenderness, 4 had moderate tenderness and no patients had severe tenderness. In group B, 15 patients had mild tenderness, 14 had moderate tenderness and 1 had severe tenderness before treatment. After treatment, 24 patients had mild tenderness, 5 had moderate tenderness and one patient had severe tenderness. Dysfunction： in group A, 12 patients had mild dysfunction, 16 had moderate dysfunction and 2 had severe dysfunction before treatment. After treatment, 24 patients had mild dysfunction, 6 had moderate dysfunction and no patients had severe dysfunction. In group B, 13 patients had mild dysfunction, 15 had moderate dysfunction and 2 had severe dysfunction before treatment. After treatment, 14 patients had mild dysfunction, 13 had moderate dysfunction and one patient had severe dysfunction. There is significant difference in total effective rate between two groups [100%（30/30） vs 86.67（26/30）, P〈0.05]. Conclusion Electroacupuncture has better therapeutic effect than that low frequency impulse therapy in the treatment of Achilles tendonitis, which can eliminate or improve symptoms, as well as heighten the quality of life for patients.展开更多
The patient with dysmenorrhea was treated by needling at Tàichōng（太冲 LR 3）, 4 times of treatment was given and the nausea, migraine and pain scale of the patient were alleviated and finally disappeared.Until...The patient with dysmenorrhea was treated by needling at Tàichōng（太冲 LR 3）, 4 times of treatment was given and the nausea, migraine and pain scale of the patient were alleviated and finally disappeared.Until the next two menstrual period, patient did not have similar complaints. The author recommended for other acupuncturists to utilize LR 3 as complementary therapy for relieving pain at primary dysmenorrhea.展开更多
Pain of supra-orbital bone is very common in clinic The author achieved significant effect in treating 56 cases of pain of supra-orbital bone by puncturing Siguan （four gates） points. The report is as follows.
Objective： To compare the different therapeutic effects of acupuncture and Phenolphthalein for constipation due to intestinal qi stagnation. Methods： A total of 50 patients with constipation due to intestinal qi sta...Objective： To compare the different therapeutic effects of acupuncture and Phenolphthalein for constipation due to intestinal qi stagnation. Methods： A total of 50 patients with constipation due to intestinal qi stagnation were randomly divided into an acupuncture group and a medication group by the random digital table, 25 cases in each group. The patients in the acupuncture group were treated by puncturing Gongsun（SP 4）, Sanyinjiao（SP 6）, Taichong（LR 3）, Zusanli（ST 36）, Shangjuxu（ST 37）, Hegu（LI 4）, Lieque（LU 7）, and Tianshu（ST 25）, once every day, and 7 d as one course, for continuous 3 courses; while the patients in the medication group were given Phenolphthalein, 7 d as one course, for continuous 3 courses. Compared the Cleveland clinic constipation score（CCS） between the two groups after one course, 3 courses and 3 months after the treatment, as well as the frequency of defecation within one week. Results： After one week of treatment, CCS scores and frequency of defecation per week were significantly changed in both groups compared with those before treatment（P〈0.05）, and CCS scores and frequency of defecation per week were improved more significantly in the patients of the medication group than in those of the acupuncture group（P〈0.01）. After 3 weeks of treatment, CCS scores and frequency of defecation per week were significantly changed in both groups compared with those before treatment（P〈0.05）, and CCS scores and frequency of defecation per week were improved more significant in the patients of the acupuncture group than in those of the medication group（P〈0.05）. Three months after the end of treatment, CCS scores and frequency of defecation per week were significantly changed in both groups compared with those before treatment（P〈0.05）, and CCS scores and frequency of defecation per week were improved more significantly in the patients of the acupuncture group than in those of the medication group（P〈0.05）. Conclusion： Acupuncture and medication are effective in the treatment of constipation due to intestinal qi stagnation. Their short-term therapeutic effect is similar, but the long-term therapeutic effect is better in the acupuncture group than in the medication group.展开更多
OBJECTIVE: To investigate the effect of low frequency electro-pulse acupuncture(EA) on blood glucose in rats with streptozotocin-induced type 2diabetes, and the possible mechanism underlying the action.METHODS: Rat mo...OBJECTIVE: To investigate the effect of low frequency electro-pulse acupuncture(EA) on blood glucose in rats with streptozotocin-induced type 2diabetes, and the possible mechanism underlying the action.METHODS: Rat models were established with high fat feeding and intraperitoneal injection of streptozotocin(STZ)(30 mg/kg). Rats with a random blood glucose > 16.7 mmol/L and blood glucose at 2 h-point of oral glucose tolerance test(OGTT) > 11.1 mmol/L were included as diabetic rats, and randomly divided into model group, EA Weiwanxiashu(EX-B 3) group, EA Zusanli(ST 36)group, glimepiride group, and EA non-acupoint group(n = 12). EA(2 Hz continuous wave, 2 m A,20 min/day, 6 days/week, 4 weeks) and intra-gastric administration of glimepiride were applied as interventions. With fasting blood glucose and OGTT tested at the end of the intervention, thestudy observed the patterns of hypoglycemic effects. For mechanism study, it observes hematoxylin and eosin staining and Masson staining of pancreas paraffin sections, protein expression of glucagon-like peptide 1 receptor(GLP-1R) in the pancreas and skeletal muscle, glucose transporter 4(GLUT4) protein expression in skeletal muscle membrane, to detect whether EA controls blood glucose via regulation of GLP-1R.RESULTS: EA Weiwanxiashu(EX-B 3) significantly increased model rats' pancreas GLP-1R, and GLUT4 of skeletal muscle membrane; the therapy significantly decreased model rats' skeletal muscle GLP-1R, restored pancreas morphology, and reduced fasting blood glucose and insulin resistance indices.CONCLUSION: EA Weiwanxiashu(EX-B 3) alone has significant effect on glycemia. EA Weiwanxiashu(EX-B 3) plus glimepiride further strengthen the effect. The regulation of the GLP-1R in pancreas and skeletal muscle might be mechanism underpinning the effect.展开更多
The purpose of this study is to investigate different factors of the artifact in surface electromyography(EMG) signal caused by functional electrical stimulation(FES). The factors investigated include the size of stim...The purpose of this study is to investigate different factors of the artifact in surface electromyography(EMG) signal caused by functional electrical stimulation(FES). The factors investigated include the size of stimulation electrode pads, the amplitude, frequency, and pulse width of the stimulation waveform and the detecting electrode points. We calculate the root mean square(RMS) of EMG signal to analyze the effect of these factors on the M-wave properties. The results indicate that the M-wave mainly depends on the stimulation amplitude and the distribution of detecting electrodes,but not on the other factors. This study can assist the reduction of artifact and the selection of detecting electrode points.展开更多
文摘AIM- To immunohistochemicaUy examine micrometastasis and VEGF-C expression in hilar bile duct carcinoma （HBDC） and to evaluate the clinical significance of the results. METHODS： A total of 361 regional lymph nodes from 25 patients with node-negative HBDC were immunostained with an antibody against cytokeratins 8 and 18 （CAM 5.2）, and immunohistochemical staining of VEGF-C was performed in 34 primary resected tumors. RESULTS： Lymph node micrometastasis was detected in 6 （24%） of the 25 patients and 10 （2.8%） of the 361 lymph nodes. Patients with micrometastasis showed significantly poorer survival rates than those without （P= 0.025）. VEGF-C expression was positive in 17 （50%） of 34 HBDC, and significantly correlated with lymph node metastasis （P=0.042） and microscopic venous invasion （P=0.035）. CONCLUSIONS： It is suggested that immunohistochemically detected lymph node micrometastasis has an impact on the outcome of HBDC. VEGF-C expression is highly correlated with lymph node metastasis in HBDC and might therefore be a useful predictor.
文摘Objective: To observe the clinical therapeutic effect of acupuncture plus intensive pulse light irradiation on chloasma. Methods: Ninety-six cases of chloasma were randomly divided into two groups, the control group of 46 cases treated by simple acupuncture and the treatment group of 50 cases treated by acupuncture and intensive pulse light irradiation. Results: The total effective rate was 89.1% and 98.0% in the control group and treatment group respectively, with a significant difference between the two groups (P<0.05). Conclusion: For chloasma, the effect of treatment with acupuncture plus intensive pulse light irradiation is superior to that with simple acupuncture.
文摘Point selection along the course of meridians is the basic principle for acupuncture prescription and selection of points. In application, there are three categories of point selection: selection of local and adjacent points; selection of distant points, and selection of symptomatic points. Based on the
文摘Objective To investigate the clinical efficacy of electroacupuncture for the treatment of Achilles tendonitis. Methods Sixty patients with Achilles tendonitis at the age of 18-55 years old were randomly divided into electroacupuncture group （group A） and low frequency impulse group （group B） according to the random number table with 30 patients in each group. Chengshan （承山 BL 57）, Ashi point, Taixi （太溪 KI 3） and Kunlun （昆仑 BL 60） were selected as main acupoints, local point selection was adopted as main method and distal point selection as supplement. Patients in group A were treated with electroacupuncture and in group B were treated with low frequency impulse therapeutic apparatus. Patients in both groups were treated for 12 times, 3 times per week in the 1st and 2nd weeks, twice a week in the 3rd and 4th weeks, and once a week in the Sth and 6th weeks, with a total of 6 weeks. The heel pain VAS scores, tenderness and efficacy of dysfunction in two groups were comparatively analyzed. Results Pain： there was significant difference in pain scores in group A before and after treatment （1.67±0.71 vs 3.03±1.81, P〈0.01）, and the difference in pain scores was not significant in group B before and after treatment （2.13±1.17 vs 2.87±1.57, P〉0.05）. Tenderness： In group A, 13 patients had mild tenderness, 15 had moderate tenderness and 2 had severe tenderness before treatment. After treatment, 26 patients had mild tenderness, 4 had moderate tenderness and no patients had severe tenderness. In group B, 15 patients had mild tenderness, 14 had moderate tenderness and 1 had severe tenderness before treatment. After treatment, 24 patients had mild tenderness, 5 had moderate tenderness and one patient had severe tenderness. Dysfunction： in group A, 12 patients had mild dysfunction, 16 had moderate dysfunction and 2 had severe dysfunction before treatment. After treatment, 24 patients had mild dysfunction, 6 had moderate dysfunction and no patients had severe dysfunction. In group B, 13 patients had mild dysfunction, 15 had moderate dysfunction and 2 had severe dysfunction before treatment. After treatment, 14 patients had mild dysfunction, 13 had moderate dysfunction and one patient had severe dysfunction. There is significant difference in total effective rate between two groups [100%（30/30） vs 86.67（26/30）, P〈0.05]. Conclusion Electroacupuncture has better therapeutic effect than that low frequency impulse therapy in the treatment of Achilles tendonitis, which can eliminate or improve symptoms, as well as heighten the quality of life for patients.
文摘The patient with dysmenorrhea was treated by needling at Tàichōng（太冲 LR 3）, 4 times of treatment was given and the nausea, migraine and pain scale of the patient were alleviated and finally disappeared.Until the next two menstrual period, patient did not have similar complaints. The author recommended for other acupuncturists to utilize LR 3 as complementary therapy for relieving pain at primary dysmenorrhea.
文摘Pain of supra-orbital bone is very common in clinic The author achieved significant effect in treating 56 cases of pain of supra-orbital bone by puncturing Siguan （four gates） points. The report is as follows.
文摘Objective： To compare the different therapeutic effects of acupuncture and Phenolphthalein for constipation due to intestinal qi stagnation. Methods： A total of 50 patients with constipation due to intestinal qi stagnation were randomly divided into an acupuncture group and a medication group by the random digital table, 25 cases in each group. The patients in the acupuncture group were treated by puncturing Gongsun（SP 4）, Sanyinjiao（SP 6）, Taichong（LR 3）, Zusanli（ST 36）, Shangjuxu（ST 37）, Hegu（LI 4）, Lieque（LU 7）, and Tianshu（ST 25）, once every day, and 7 d as one course, for continuous 3 courses; while the patients in the medication group were given Phenolphthalein, 7 d as one course, for continuous 3 courses. Compared the Cleveland clinic constipation score（CCS） between the two groups after one course, 3 courses and 3 months after the treatment, as well as the frequency of defecation within one week. Results： After one week of treatment, CCS scores and frequency of defecation per week were significantly changed in both groups compared with those before treatment（P〈0.05）, and CCS scores and frequency of defecation per week were improved more significantly in the patients of the medication group than in those of the acupuncture group（P〈0.01）. After 3 weeks of treatment, CCS scores and frequency of defecation per week were significantly changed in both groups compared with those before treatment（P〈0.05）, and CCS scores and frequency of defecation per week were improved more significant in the patients of the acupuncture group than in those of the medication group（P〈0.05）. Three months after the end of treatment, CCS scores and frequency of defecation per week were significantly changed in both groups compared with those before treatment（P〈0.05）, and CCS scores and frequency of defecation per week were improved more significantly in the patients of the acupuncture group than in those of the medication group（P〈0.05）. Conclusion： Acupuncture and medication are effective in the treatment of constipation due to intestinal qi stagnation. Their short-term therapeutic effect is similar, but the long-term therapeutic effect is better in the acupuncture group than in the medication group.
基金Supported by Ministry of Education of the People's Republic of China:a Mechanism Study of Electro-Acupuncture EX-B3's Effect on Pancreas PDX-1 of Goto-Kakizaki Rats(No.313010)
文摘OBJECTIVE: To investigate the effect of low frequency electro-pulse acupuncture(EA) on blood glucose in rats with streptozotocin-induced type 2diabetes, and the possible mechanism underlying the action.METHODS: Rat models were established with high fat feeding and intraperitoneal injection of streptozotocin(STZ)(30 mg/kg). Rats with a random blood glucose > 16.7 mmol/L and blood glucose at 2 h-point of oral glucose tolerance test(OGTT) > 11.1 mmol/L were included as diabetic rats, and randomly divided into model group, EA Weiwanxiashu(EX-B 3) group, EA Zusanli(ST 36)group, glimepiride group, and EA non-acupoint group(n = 12). EA(2 Hz continuous wave, 2 m A,20 min/day, 6 days/week, 4 weeks) and intra-gastric administration of glimepiride were applied as interventions. With fasting blood glucose and OGTT tested at the end of the intervention, thestudy observed the patterns of hypoglycemic effects. For mechanism study, it observes hematoxylin and eosin staining and Masson staining of pancreas paraffin sections, protein expression of glucagon-like peptide 1 receptor(GLP-1R) in the pancreas and skeletal muscle, glucose transporter 4(GLUT4) protein expression in skeletal muscle membrane, to detect whether EA controls blood glucose via regulation of GLP-1R.RESULTS: EA Weiwanxiashu(EX-B 3) significantly increased model rats' pancreas GLP-1R, and GLUT4 of skeletal muscle membrane; the therapy significantly decreased model rats' skeletal muscle GLP-1R, restored pancreas morphology, and reduced fasting blood glucose and insulin resistance indices.CONCLUSION: EA Weiwanxiashu(EX-B 3) alone has significant effect on glycemia. EA Weiwanxiashu(EX-B 3) plus glimepiride further strengthen the effect. The regulation of the GLP-1R in pancreas and skeletal muscle might be mechanism underpinning the effect.
基金State Key Laboratory of Robotics and Systemgrant number:SKLRS-2012-ZD-04+1 种基金Natural Science Foundation of Shanghaigrant number:14ZR1421300
文摘The purpose of this study is to investigate different factors of the artifact in surface electromyography(EMG) signal caused by functional electrical stimulation(FES). The factors investigated include the size of stimulation electrode pads, the amplitude, frequency, and pulse width of the stimulation waveform and the detecting electrode points. We calculate the root mean square(RMS) of EMG signal to analyze the effect of these factors on the M-wave properties. The results indicate that the M-wave mainly depends on the stimulation amplitude and the distribution of detecting electrodes,but not on the other factors. This study can assist the reduction of artifact and the selection of detecting electrode points.
