《切割》

本书阐述了五大切割操作：感性切割实现差异化、品类切割实现以弱胜强、市场切割实现有效分销、人群切割实现费用投入高效率、品牌切割实现低成本快速构建品牌。作者路长全，曾任伊利集团营销副总、巨能实业集团副总裁、（意）赞邦集团中国区营销总监、中国远大营销策划副总。创造一系列经典的成功案例。为数百家企业和机构进行营销咨询和管理服务，凭借对国际经典管理理论的深刻理解和对中国市场、文化的透彻剖析与把握，理性而大胆地帮助他所服务的企业摆脱巨大困境，走向发展之路。

《献给哈里斯夫人的鲜花》《切割》等获得英国剧院奖最高奖项

英国剧院奖旨在表彰英国所有的剧院中在表演、制作和管理中拥有最杰出成就的演员、作品及其他从业人员。今年，在最新公布的获奖名单中，《切割》（Cuttin’It）获得最佳新剧目奖。

《切割》

导演:詹妮·坎普贞编剧:詹妮·坎普贞 苏珊娜·摩尔演员:梅格·瑞恩 马克·鲁法罗 凯文·培根 詹尼佛·杰森·利 尼克·达米西类型:犯罪/悬疑片级:R级片长1小时38分钟首映:2003年10月22日(纽约/洛杉矶)

《切割营销》

本书作者路长全是中国最具价值的营销实战专家，一位用20年实践推动企业成长的营销巨匠，中国一系列成功营销的设计师和操刀者，中国营销理论的思想家，现任北京赞伯营销管理咨询有限公司董事长，清华大学特聘教授，海尔商学院客座教授，曾任伊利集团营销副总，中国远大营销策划副总，中央电视台广告部策略顾问等。

Three-dimensional orebody modelling and intellectualized longwall mining for stratiform bauxite deposits

Acoupled biharmonic spline and linear interpolation algorithm was proposed to create a three-dimensional smooth deposit model with minimal curvature containing grade and position data. To obtain the optimal technical parameters, such as cuttingheight and drum diameter, a virtual longwall mining procedure was modelled by simulating the actual fully mechanized longwall mining process. Based on the above work, a bauxite deposit in a longwall mining panel was modelled by scattered grade data from ores sampled on the entry wall. The deposit was then demarcated by industrial indexes and sliced according to the virtual longwallmining procedure. The results show that the proposed interpolation algorithm can depict the stratiform structure of bauxite depositsand that the uncovered bauxite deposit has high proportions of high-grade and rich ore. The ranges of optimal cutting height and drum diameters are 1.72-2.84 m and 1.42-1.72 m, respectively. Finally, an intellectualized longwall mining procedure was designed to guide the mining process with the lowest dilution and loss rates.

Studies on Contamination of Cytoplasm DNA and Its Control in Plant Chromosone Microdissection

染色体微切割和微克隆已成为复杂基因组研究的有效途径 ,但是操作过程中的核外DNA的污染一直是令人担心的问题。通过研究植物染色体微切割 (微分离 )和微切割的染色体DNA扩增过程中细胞质DNA的污染问题 ,表明目前常用的植物染色体微切割过程中 ,细胞质DNA的污染几乎难以避免 ,并提出了一个改进的降低细胞质DNA污染的方法 ,对如何控制细胞质DNA的污染进行了详细的讨论。

Microdissection of Haynaldia villosa Telosome 6VS and Cloning of Species-specific DNA Sequences

The material T240_6 derived from SC 2 young embryo of the combination CA9211/RW15 (6D/6V alien substitution) was telosomic substitution line of 6VS identified by GISH (genomic in situ hybridization) analysis. The 6VS was microdissected with a needle and transferred into a 0.5 mL Ep tube. In the 'single tube', all the subsequence steps were conducted. After two round of LA (Linker adaptor)_PCR amplification, the size of PCR bands ranged from 100 to 3 000 bp, with predominate bands 600-1 500 bp. The products were confirmed by Southern blotting analysis using Haynaldia villosa (L.) Schur. genomic DNA labeled with 32 P as probe. The PCR products were purified and ligated into clone vector-pGEM_T easy vector. Then, the plasmids were transformed into competence E. coli JM109 with cool CaCl 2. It was estimated that there were more than 17 000 white clones in the library. The size of insert fragments distributed from 100-1 500 bp, with average of 600 bp. Using H. villosa genomic DNA as probe, dot blotting results showed that 37% clones displayed strong and medium positive signals, and 63% clones had faint or no signals. It is demonstrated that there were about 37% repeat sequence clones and 67% single/unique sequence clones in the library. Eight H. villosa_specific clones were screened from the library, and two clones pHVMK22 and pHVMK134 were used for RFLP analysis and sequencing. Both of them were H. villosa specific clones. The pHVMK22 was a unique sequence clone, and the pHVMK134 was a repeat sequence clone. When the pHVMK22 was used as a probe for Southern hybridization, all the powdery mildew resistance materials showed a special band of 2 kb, while all the susceptible ones not. The pHVMK22 may be applied to detect the existence of Pm21.

英国脱欧小说中的对立主题研究

在英国宣布脱欧后,英国文学界涌现出一批涉及脱欧事件的作品。这些作品虽然创作方式不同、讨论话题各异,但普遍涉及对立主题。其中,今与昔、国家主义与世界主义以及城市与乡村这三组对立在《秋》《切割》《蟑螂》《大麦地里的一切》等脱欧小说中频繁出现。这些对立主题不仅反映了当下存在于英国社会中的诸多现状,而且还暗示当代英国作家试图寻求超越此类对立和为英国社会发展谋求出路的尝试。对于这些普遍存在于脱欧文学作品中的对立主题,我们不仅要看到对立冲突的表象,也要深入思考其背后深藏的问题。作家在呈现这些对立主题时往往不是简单地以揭露一个或一类现象为目的,而是试图超越这些出现在文本中的对立表征,达成某种更高层面上的统一。这反映了他们对时代不同程度上的理解与关怀。英国脱欧这样一个特殊而具有划时代意义的历史事件使许多时代问题得到集中爆发与释放,这些或新或旧的问题被以不同的方式反映或表达在文学作品中。作家在对这些问题的揭露与讨论中试图给出自己的“药方”,表达一种感性的期望。这是当代脱欧文学的特性和魅力所在,同时也是其与以往文学的共性所在。

WEDM oriented micro gear design and mesh simulation

For the design of gears manufactured with wire electrical discharge machining （WEDM） technology, determination of the primary gear parameters is discussed considering the characteristics of the machining method. Some constraint conditions on gear parameters are abnegated, which makes micro gear design more flexible. Based on gear mesh theory, the algorithm of generating gear tooth profiles is studied, which includes involute and non-involute curve segments. The phenomena of tooth profile interferences during gear mesh are analyzed, and a gear mesh simulation algorithm is designed. Based on ACIS, the WEDM oriented software for the design and mesh simulation of micro gears is developed, by which the modeling, mesh simulation and interference check can be implemented. An experiment is carried out to design and manufacture a pair of micro involute gears, and the proposed method is proved feasible.

FOUR-AXIS WIRE-EDM SIMULATION OF COMPLICATED MODELS

In the verification of wire electrical discharge machining （EDM）, the motion and the performance of the wire-EDM system are analyzed. The maximum inclining angle of the wire is calculated. The relevant judgment methods are used for the collision between the wire, the fixture, and the machining table. In the wire-EDM simulation, the generated solid model can he used to investigate programming results and to check the machining accuracy. The generation algorithm for the solid model in the simulation is solved based on Boolean operations. The wire swept volume for each cutting step is united to form the entire wire swept volume. Through Boolean subtraction between the stock model and the entire wire swept volume, the solid model in the wire-EDM simulation is generated. The method is also suitable for the wire path intersection occurred in cutting cone-shaped models. Finally, experiments are given to prove the method.

Laser Capture Microdissection Combined with RNA in vitro Linear Amplification Detecting the Relevant Genes of Bladder Cancer

Objective： Laser capture microdisection has become indispensable to the analysis of the difference of gene expression between human bladder transitional cell and bladder transitional cell carcinoma （BTCC）. However, to obtain sufficient RNA from laser-capture microdissected cells is quite difficult. The study was designed to determinc a feasible technical routine to isolate transitional cells from bladder membrane, separate carcinoma cclls from stromal cells and to amplify the RNA isolated from laser-capture microdissected cells. Methods： Bladder transitional cell were obtained from frozen sections of bladder membrane applying LCM, by the same token, BTCC cells from frozen sections of BTCC tissue. Then RNA was extracted and linearly amplified in vitro. The expression levels of β-actin in primary total RNA and amplified RNA were detected using RT-PCR. Results： That RNA integrity was good after LCM was confirmed by control experiment Ⅰ; By control experiment Ⅱ, the correlation between the number of LCM-shooting and RNA quantity undcr arranged conditions was preliminarily confirmed. About 0.5-2.5kb RNA fragments were obtained after RNA amplification and β-actin levels were integral. Conclusion： Laser capture microdissection combined with RNA linear amplification in vitro can be successfully applied to obtain pure objective cells for research. The integrity of the amplified RNA is good and can be employed in further research.

Recovery of high purity silicon from SoG crystalline silicon cutting slurry waste

The composition and size distribution of cutting waste were characterized. The Si-rich powders were obtained from the cutting waste using a physical sedimentation process, and then further purified by removing impurity using acid leaching. The effects of process parameters such as acid leaching time, temperature and the ratio of solid to liquid on the purification efficiency were investigated, and the parameters were optimized. Afterwards, the high-purity Si ingot was obtained by melting the Si-rich powders in vacuum furnace. Finally, the high purity Si with 99.96%Si, 1.1×10^-6 boron （B）, and 4.0×10^-6 phosphorus （P） were obtained. The results indicate that it is feasible to extract high-purity Si, and further produce SoG-Si from the cutting slurry waste.

Digestive tract reconstruction using isoperistaltic jejunumlater-cut overlap method after totally laparoscopic total gastrectomy for gastric cancer: Short-term outcomes and impact on quality of life

AIM To evaluate the short-term outcomes and quality of life(Qo L) in gastric cancer patients undergoing digestive tract construction using the isoperistaltic jejunum-latercut overlap method(IJOM) after totally laparoscopic total gastrectomy(TLTG).METHODS A total of 507 patients who underwent laparoscopic gastrectomy(D2) from January 2014 to March 2016 were originally included in the study. The patients were divided into two groups to undergo digestive tract construction using either IJOM after TLTG(group T, n = 51) or Roux-en-Y anastomosis after laparoscopic-assisted total gastrectomy(LATG)(group A, n = 456). The short-term outcomes and Qo L were compared between the two groups after 1:2 propensity-score matching(PSM). We used a questionnaire to assess Qo L.RESULTS Before matching, age, sex, tumor size, tumor location, preoperative albumin and blood loss were significantly different between the two groups(P < 0.05). After PSM, the patients were well balanced in terms of their clinicopathological characteristics, although both blood loss and in-hospital postoperative days in group T were significantly lower than those in group A(P < 0.05). After matching, group T reported better Qo L in the domains of pain and dysphagia. Among the items evaluating pain and dysphagia, group T tended to report better Qo L("Have you felt pain" and "Have you had difficulty eating solid food")(P < 0.05).CONCLUSION The IJOM for digestive tract reconstruction after TLTG is associated with reduced blood loss and less pain and dysphagia, thus improving Qo L after laparoscopic gastrectomy.

New Cutting Force Modeling Approach for Flat End Mill

A new mechanistic cutting force model for flat end milling using the instantaneous cutting force coefficients is proposed. An in-depth analysis shows that the total cutting forces can be separated into two terms: a nominal component independent of the runout and a perturbation component induced by the runout. The instantaneous value of the nominal component is used to calibrate the cutting force coefficients. With the help of the perturbation component and the cutting force coefficients obtained above, the cutter runout is identified. Based on simulation and experimental results, the validity of the identification approach is demonstrated. The advantage of the proposed method lies in that the calibration performed with data of one cutting test under a specific regime can be applied for a great range of cutting conditions.

Mechanical model of breaking rock and force characteristic of disc cutter

According to the cutting characteristics of progressive spiral movement by rotary cutting of the disc cutter, using the broken theory of interaction of compression and shearing, the three-axis force rotary cutting mechanical model of disc cutter was established and the influence of installation radius, the phase difference and the cutter space on the mechanics of disc cutter were analyzed. The results show that on the same radial line of tunneling interface, the boring distance of cutting tools installed on a different radius is not equal. The cutting radial line of tunneling interface is a polyline and its height is determined by phase angle and penetration of cutting tools. Both phase difference and the installation radius between adjacent disc cutters have little effect on the vertical force and rolling force, but increase with the increase in cutter spacing. In addition, when increasing phase difference and cutter space bilaterally, and reducing installation radius simultaneously, the lateral force would be improved. Related results have been verified onl O0 t rotary tool cutting test platform.

Analyses and predictions of rock cuttabilities under different confining stresses and rock properties based on rock indentation tests by conical pick

The rock indentation tests by a conical pick were conducted to investigate the rock cuttability correlated to confining stress conditions and rock strength.Based on the test results,the regression analyses,support vector machine(SVM)and generalized regression neural network(GRNN)were used to find the relationship among rock cuttability,uniaxial confining stress applied to rock,uniaxial compressive strength(UCS)and tensile strength of rock material.It was found that the regression and SVM-based models can accurately reflect the variation law of rock cuttability,which presented decreases followed by increases with the increase in uniaxial confining stress and the negative correlation to UCS and tensile strength of rock material.Based on prediction models for revealing the optimal stress condition and determining the cutting parameters,the axial boom roadheader with many conical picks mounted was satisfactorily utilized to perform rock cutting in hard phosphate rock around pillar.

Cutting-balloon angioplasty before drug-eluting stent implantation for the treatment of severely calcified coronary lesions

Background Severely calcified coronary lesions respond poorly to balloon angioplasty, resulting in incomplete and asymmetrical stent expansion. Therefore, adequate plaque modification prior to drug-eluting stent （DES） implantation is the key for calcified lesion treat- ment. This study was to evaluate the safety and efficacy of cutting balloon angioplasty for severely calcified coronary lesions. Methods Ninety-two consecutive patients with severely calcified lesions （defined as calcium arc 〉 180% calcium length ratio 〉 0.5） treated with bal- loon dilatation before DES implantation were randomly divided into two groups based on the balloon type： 45 patients in the conventional balloon angioplasty （BA） group and 47 patients in the cutting balloon angioplasty （CB） group. Seven cases in BA group did not satisfactorily achieve dilatation and were transferred into the CB group. Intravascular ultrasound （IVUS） was performed before balloon dilatation and after stent implantation to obtain qualitative and quantitative lesion characteristics and evaluate the stent, including minimum lumen cross-sectional area （CSA）, calcified arc and length, minimum stent CSA, stent apposition, stent symmetry, stent expansion, vessel dissection, and branch vessel jail. In-hospital, 1-month, and 6-month major adverse cardiac events （MACE） were reported. Results There were no statistical differences in clinical characteristics between the two groups, including calcium arc （222.2° ± 22.2° vs. 235.0° ± 22.1 °, p=0.570）, calcium length ratio （0.67 ± 0.06 vs. 0.77± 0.05, P = 0.130）, and minimum lumen CSA before PCI （2.59 ±0.08 mm2 vs. 2.52 ± 0.08 mm2, P = 0.550）. After stent implantation, the final minimum stent CSA （6.26 ± 0.40 mm2 vs. 5.03 ± 0.33 mm2; P = 0.031） and acute lumen gain （3.74 ±0.38 mm2 w. 2.44 ± 0.29 mm2, P = 0.015） were significantly larger ila the CB group than that of the BA group. There were not statis tically differences in stent expansion, stent symmetry, incomplete stent apposition, vessel dissection and branch vessel jail between two groups. The 30-day and 6-month MACE rates were also not different. Conclusions Cutting balloon angioplasty before DES implantation in severely calcified lesions appears to be more efficacies including significantly larger final stent CSA and larger acute lumen gain, without increasing complications during operations and the MACE rate in 6-month.

Review on non-conventional machining of shape memory alloys

Shape memory alloys （SMAs） are the developing advanced materials due to their versatile specific properties such as pseudoelasticity, shape memory effect （SME）, biocompatibility, high specific strength, high corrosion resistance, high wear resistance and high anti-fatigue property. Therefore, the SMAs are used in many applications such as aerospace, medical and automobile. However, the conventional machining of SMAs causes serious tool wear, time consuming and less dimensional deformity due to severe strain hardening and pseudoelasticity. These materials can be machined using non-conventional methods such as laser machining, water jet machining （WJM） and electrochemical machining （ECM）, but these processes are limited to complexity and mechanical properties of the component. Electrical discharge machining （EDM） and wire EDM （WEDM） show high capability to machine SMAs of complex shapes with precise dimensions. The aim of this work is to present the consolidated references on the machining of SMAs using EDM and WEDM and subsequently identify the research gaps. In support to these research gaps, this work has also evolved the future research directions.

Effect of landform on aerodynamic performance of high-speed trains in cutting under cross wind

The effects of the different landforms of the cutting leeward on the aerodynamic performance of high-speed trains were analyzed based on the three-dimensional, steady, and incompressible Navier-Stokes equation and k-e double-equation turbulent model. Results show that aerodynamic forces increase with the cutting leeward slope decreasing. The maximum adding value of lateral force, lift force, and overturning moment are 147%, 44.3%, and 107%, respectively, when the slope varies from 0.67 to -0.67, and the changes in the cutting leeward landform have more effects on the aerodynamic performance when the train is running in the line No. 2 than in the line No. 1. The aerodynamic forces, except the resistance force, sharply increase with the slope depth decreasing. By comparing the circumstance of the cutting depth H=-8 m with that of H=8 m, the resistance force, lateral force, lift force, and overturning moment increase by 26.0%, 251%, 67.3% and 177%, respectively. With the wind angle increasing, the resistance force is nonmonotonic, whereas other forces continuously rise. Under three special landforms, the changes in the law of aerodynamic forces with the wind angle are almost similar to one another.

Reasonable location parameters of pick and nozzle in combined cutting system

The drum shearer and high pressure water jet combined cutting system is an effective technology to cut hard coal-rock, but one problem of the technology is the choice of pick and nozzle location parameters. In order to solve the problem, the process and mechanism of combined cutting arc studied and mining seepage catastrophe theory is used to construct the mathematic and physical model of combined cutting hard coal-rock. Based on the model and detailed analysis of combined cutting mechanism, the single pick and nozzle combined cutting test-bed is built to test the main pick and nozzle location parameters of drum shearer and water jet combined cutting system. Test results show that the best vertical distance between the pick-tip and nozzle center point is the sum of cutting thickness and proper target distance in the Y axial direction; the best horizontal distance is the length between pick-tip point and coal-rock surface bursting crack point in the X axial direction. The best incident angle of water jet should be the same as the bursting crack line＇s angle in theory, but considering other important factors comprehensively, it is more reasonable when the incident angle of water jet is 90°.