A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for ...A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for use in the sandwich ELISA.The MAb,designated as 5B5 was specific to VP7 and belongs to IgG2a subclass and was selected for the development of the sELISA in this study.The MAb had a titer of 1:25 with BTV and 1:2 with the rVP7 protein.The sELISA is based on capturing of BTV antigen with VP7 specific MAb followed by detection using BTV polyclonal antiserum raised in rabbits.The assay was evaluated with six cell culture adapted serotypes of BTV that have been isolated from India,1,2,15,17,18 and 23.The assay could detect BTV antigen as early as day 8 in blood.It was also successfully applied for the detection of BTV group specific antigen in clinical samples of blood,washed RBCs,buffy coat and plasma.A total of 102 field samples from animals,suspected of being infected with BTV,were tested and 29.42% were positive.The blood samples were also amplified in cell culture which improved the sensitivity of the assay.Results confirmed that the sELISA is rapid and specific.展开更多
In the present study,a total of 24 MAbs were produced against bluetongue virus (BTV) by polyethyleneglycol (PEG) mediated fusion method using sensitized lymphocytes and myeloma cells. All these clones were characteriz...In the present study,a total of 24 MAbs were produced against bluetongue virus (BTV) by polyethyleneglycol (PEG) mediated fusion method using sensitized lymphocytes and myeloma cells. All these clones were characterized for their reactivity to whole virus and recombinant BTV-VP7 protein,titres,isotypes and their reactivity with 24 BTV-serotype specific sera in cELISA. Out of 24 clones,a majority of them (n = 18) belong to various IgG subclasses and the remaining (n = 6) to the IgM class. A panel of eight clones reactive to both whole BTV and purified rVP7 protein were identified based on their reactivity in iELISA. For competitive ELISA,the clone designated as 4A10 showed better inhibition to hyperimmune serum of BTV serotype 23. However,this clone showed a variable percent of inhibition ranging from16.6% with BTV 12 serotype to 78.9% with BTV16 serotype using 24 serotype specific sera of BTV originating from guinea pig at their lowest dilutions. From the available panel of clones,only 4A10 was found to have a possible diagnostic application.展开更多
This paper deals with the computer modeling of structures starting from a point cloud. The CCTV （China Central Television） tower headquarters is the case for study because the shape of this building is non-stellar, ...This paper deals with the computer modeling of structures starting from a point cloud. The CCTV （China Central Television） tower headquarters is the case for study because the shape of this building is non-stellar, concave and multi-connected. It is composed of sowns and chains. The sown is the representation of a horizontal plane formed by dense points. The chain is a planar path modeled by rare points. The CCTV structure is defined only by the three orthogonal Cartesian coordinates of the points. The proposed computer modeling uses a sequence of procedures and the desired outputted 3D model is consistent. The first procedure is devoted to attributing points to their voxel and to estimating three values needed afterwards. The second procedure is devoted to analyzing clusters vertically and horizontally, to preliminarily distinguishing chains from sowns and to generating relational matching. The third procedure is devoted to building closed paths between all chains and all their projections on sowns. The fourth procedure is devoted to connecting points with triangles. The fifth procedure, still being implemented, is devoted to interpolating triangles with triangular splines. The results show it is possible to achieve the 3D model using the above mentioned procedures. These procedures are written, implemented and tested and they form a library of people＇s own software. The code is written using Matlab. It is not possible to obtain the required 3D model if the procedures are applied in the wrong order or one step is skipped. To conclude, it is possible to obtain the computer model of the CCTV using the provided sequence of procedures.展开更多
In a comedy show about telephone scams staged during the 2017 Spring Festival Gala on Beijing TV,the voice of the skit’s swindler had a Henan provincial accent.A lawyer from Henan has since filed a lawsuit accusing t...In a comedy show about telephone scams staged during the 2017 Spring Festival Gala on Beijing TV,the voice of the skit’s swindler had a Henan provincial accent.A lawyer from Henan has since filed a lawsuit accusing the show’s director and actors and also the TV station of regional discrimination and harming the reputation of Henan people.展开更多
文摘A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for use in the sandwich ELISA.The MAb,designated as 5B5 was specific to VP7 and belongs to IgG2a subclass and was selected for the development of the sELISA in this study.The MAb had a titer of 1:25 with BTV and 1:2 with the rVP7 protein.The sELISA is based on capturing of BTV antigen with VP7 specific MAb followed by detection using BTV polyclonal antiserum raised in rabbits.The assay was evaluated with six cell culture adapted serotypes of BTV that have been isolated from India,1,2,15,17,18 and 23.The assay could detect BTV antigen as early as day 8 in blood.It was also successfully applied for the detection of BTV group specific antigen in clinical samples of blood,washed RBCs,buffy coat and plasma.A total of 102 field samples from animals,suspected of being infected with BTV,were tested and 29.42% were positive.The blood samples were also amplified in cell culture which improved the sensitivity of the assay.Results confirmed that the sELISA is rapid and specific.
文摘In the present study,a total of 24 MAbs were produced against bluetongue virus (BTV) by polyethyleneglycol (PEG) mediated fusion method using sensitized lymphocytes and myeloma cells. All these clones were characterized for their reactivity to whole virus and recombinant BTV-VP7 protein,titres,isotypes and their reactivity with 24 BTV-serotype specific sera in cELISA. Out of 24 clones,a majority of them (n = 18) belong to various IgG subclasses and the remaining (n = 6) to the IgM class. A panel of eight clones reactive to both whole BTV and purified rVP7 protein were identified based on their reactivity in iELISA. For competitive ELISA,the clone designated as 4A10 showed better inhibition to hyperimmune serum of BTV serotype 23. However,this clone showed a variable percent of inhibition ranging from16.6% with BTV 12 serotype to 78.9% with BTV16 serotype using 24 serotype specific sera of BTV originating from guinea pig at their lowest dilutions. From the available panel of clones,only 4A10 was found to have a possible diagnostic application.
文摘This paper deals with the computer modeling of structures starting from a point cloud. The CCTV （China Central Television） tower headquarters is the case for study because the shape of this building is non-stellar, concave and multi-connected. It is composed of sowns and chains. The sown is the representation of a horizontal plane formed by dense points. The chain is a planar path modeled by rare points. The CCTV structure is defined only by the three orthogonal Cartesian coordinates of the points. The proposed computer modeling uses a sequence of procedures and the desired outputted 3D model is consistent. The first procedure is devoted to attributing points to their voxel and to estimating three values needed afterwards. The second procedure is devoted to analyzing clusters vertically and horizontally, to preliminarily distinguishing chains from sowns and to generating relational matching. The third procedure is devoted to building closed paths between all chains and all their projections on sowns. The fourth procedure is devoted to connecting points with triangles. The fifth procedure, still being implemented, is devoted to interpolating triangles with triangular splines. The results show it is possible to achieve the 3D model using the above mentioned procedures. These procedures are written, implemented and tested and they form a library of people＇s own software. The code is written using Matlab. It is not possible to obtain the required 3D model if the procedures are applied in the wrong order or one step is skipped. To conclude, it is possible to obtain the computer model of the CCTV using the provided sequence of procedures.
文摘In a comedy show about telephone scams staged during the 2017 Spring Festival Gala on Beijing TV,the voice of the skit’s swindler had a Henan provincial accent.A lawyer from Henan has since filed a lawsuit accusing the show’s director and actors and also the TV station of regional discrimination and harming the reputation of Henan people.
