Allelopathic potentials of aqueous water extracts from residues of sorghum stem, maize inflorescence and rice husks on the germination of soybeans （Glycine max. L.） were investigated. The result showed that the extr...Allelopathic potentials of aqueous water extracts from residues of sorghum stem, maize inflorescence and rice husks on the germination of soybeans （Glycine max. L.） were investigated. The result showed that the extracts brought about a considerable inhibition in the germination of soybean seeds, reductions in the radicle and plumule lengths of soybean seedlings. Whereas, the extracts of maize inflorescence and rice husk had more inhibitory effects on the growth of soybean radicle, all the three extracts demonstrated pronounced inhibitory effects on the growth of the plumule. The retardation of soybean germination, radicle and plumule growths were concentration dependents as the degree of retardations increases with increase in the concentration of the extracts. Statistical analysis （ANOVA, P = 0.05） revealed that there were no significant differences in the germination, radicle and plumule growths at 24 and 48 h experimental time when compared to the control. However, statistical differences abound in the growth parameters at 72 and 144 h experimental time.展开更多
In the present work, we investigated the effect of Mucuna deeringiana defatted bran on seed germination of soybean （Glycine max L. Merrill）. For this, the defatted bran was obtained from different operational condit...In the present work, we investigated the effect of Mucuna deeringiana defatted bran on seed germination of soybean （Glycine max L. Merrill）. For this, the defatted bran was obtained from different operational conditions using CO2 under pressurized conditions as solvent. Oil extractions were carried out in a laboratorial scale unit at temperatures ranging from 310 to 330 K, pressure from 15 to 25 MPa and solvent flow set in 3 mL min^-1. The experiment was conducted in a germination chamber （25℃, 12：12 h light：dark photoperiod）, and the germination rate and germination speed index （GSI） were evaluated for seven days. The results showed that the oil extraction with supercritical CO2 concentrates the allelochemical L-3,4-dihydroxyphenylalanine （L-DOPA） in bran with levels from 1.86% to 4.65%. Subsequent experiments revealed that the rate of soybean seed germination was not affected by defatted bran whereas the GSI increased significantly after treatment. In brief, we concluded that the extraction process with supercritical CO2 is efficient to obtain L-DOPA of Mucuna, which influences the soybean seed germination.展开更多
Each component of rice bran fermentation(RBF)of Bacillus natto was extracted and its antioxidant activity was tested.By dint of thin layer chromatography,the antioxidant components were separated.The sample isolated f...Each component of rice bran fermentation(RBF)of Bacillus natto was extracted and its antioxidant activity was tested.By dint of thin layer chromatography,the antioxidant components were separated.The sample isolated from TLC for ethyl acetate phase and ether phase was analyzed by GC-MS.Results indicated that the antioxidant activity of large components in rice bran fermentation was low.The constituents of extracted phase had antioxidant activity apart from the petroleum ether phase.The constituents of ethyl acetate phase and Ether phase had best antioxidant activity,163 and 168 kU/g,respectively.The main composition of ethyl acetate phase was allyl group methyl-thioether and the main composition of ether phase included 3,5-dimethyl phenol,2,4-dipl-tert-Butyl and Fluazifop.展开更多
文摘Allelopathic potentials of aqueous water extracts from residues of sorghum stem, maize inflorescence and rice husks on the germination of soybeans （Glycine max. L.） were investigated. The result showed that the extracts brought about a considerable inhibition in the germination of soybean seeds, reductions in the radicle and plumule lengths of soybean seedlings. Whereas, the extracts of maize inflorescence and rice husk had more inhibitory effects on the growth of soybean radicle, all the three extracts demonstrated pronounced inhibitory effects on the growth of the plumule. The retardation of soybean germination, radicle and plumule growths were concentration dependents as the degree of retardations increases with increase in the concentration of the extracts. Statistical analysis （ANOVA, P = 0.05） revealed that there were no significant differences in the germination, radicle and plumule growths at 24 and 48 h experimental time when compared to the control. However, statistical differences abound in the growth parameters at 72 and 144 h experimental time.
文摘In the present work, we investigated the effect of Mucuna deeringiana defatted bran on seed germination of soybean （Glycine max L. Merrill）. For this, the defatted bran was obtained from different operational conditions using CO2 under pressurized conditions as solvent. Oil extractions were carried out in a laboratorial scale unit at temperatures ranging from 310 to 330 K, pressure from 15 to 25 MPa and solvent flow set in 3 mL min^-1. The experiment was conducted in a germination chamber （25℃, 12：12 h light：dark photoperiod）, and the germination rate and germination speed index （GSI） were evaluated for seven days. The results showed that the oil extraction with supercritical CO2 concentrates the allelochemical L-3,4-dihydroxyphenylalanine （L-DOPA） in bran with levels from 1.86% to 4.65%. Subsequent experiments revealed that the rate of soybean seed germination was not affected by defatted bran whereas the GSI increased significantly after treatment. In brief, we concluded that the extraction process with supercritical CO2 is efficient to obtain L-DOPA of Mucuna, which influences the soybean seed germination.
基金Supported by Young Pioneer Teacher Fund of Xinyang Normal University in Henan Province(2006-014)Natural Scientific Fund of Educational Bureau in Henan Province(2011B180050)
文摘Each component of rice bran fermentation(RBF)of Bacillus natto was extracted and its antioxidant activity was tested.By dint of thin layer chromatography,the antioxidant components were separated.The sample isolated from TLC for ethyl acetate phase and ether phase was analyzed by GC-MS.Results indicated that the antioxidant activity of large components in rice bran fermentation was low.The constituents of extracted phase had antioxidant activity apart from the petroleum ether phase.The constituents of ethyl acetate phase and Ether phase had best antioxidant activity,163 and 168 kU/g,respectively.The main composition of ethyl acetate phase was allyl group methyl-thioether and the main composition of ether phase included 3,5-dimethyl phenol,2,4-dipl-tert-Butyl and Fluazifop.
