The annual life cycle of the brown seaweed Undariapinnatifida （Harvey） Suringer comprises a macroscopic diploid sporophyte stage and a microscopic haploid gametophyte stage. In 2011, an unusual zoospore-derived mono...The annual life cycle of the brown seaweed Undariapinnatifida （Harvey） Suringer comprises a macroscopic diploid sporophyte stage and a microscopic haploid gametophyte stage. In 2011, an unusual zoospore-derived monoecious gametophyte isolate （designated as line 10-5-3） of U. pinnatifida was observed. To understand this phenomenon, a comprehensive screening of eighty-two previously identified male gametophyte cultures, isolated from three randomly selected cultivars （lines 10, 7, and 5） was performed. Thirty-six of the isolates developed both antheridia and oogonia on the same filamentous fragment in a standard gametogenesis test （SGT： 18℃, 60 umol photons/（m2.s））. Selfing of the monoecious gametophyte or crossing it with a normal male gametophyte both gave rise to morphologically normal sporophytic offspring. However, crossing resulted in a much higher fertilization rate （89.7%）. The hybrid and selfed sporophytic offspring were grown to maturity in flow tanks at an ambient temperature of 10-18℃ over a period of 69 days. Active zoospores were released from both types of mature sporophylls. The majority of these developed into male gametophytes, while 15%-20% developed into the observed monoecious structures on the same filament. Using PCR amplification it was found that all the monoecious gametophyte isolates and the sporophytic offspring resulting from the selfing and crossing lacked the femalelinked microsatellite sequence （a part of the locus Up-AC-2A8, GenBank accession No. AY738602.1）, indicating their male nature. U.pinnatifida is an invasive species in some regions and the implications of the above findings for this species in nature are briefly discussed.展开更多
Progeny testing of oil palm interspecific hybrids was conducted in four trials in Kluang, Ulu Paka, Teluk Intan and Carey Island, Malaysia. The interspecific hybrids （O × P） were created according to the North ...Progeny testing of oil palm interspecific hybrids was conducted in four trials in Kluang, Ulu Paka, Teluk Intan and Carey Island, Malaysia. The interspecific hybrids （O × P） were created according to the North Carolina Model I （NCM I） mating design, using Elaeis oleifera （0） and Elaeis guineensis var. pisifera （P） as the maternal and paternal parents, respectively. Differences among O, P and O-within-P were determined by the performance （bunch yield, components and vegetative traits） of the progenies. There were significant differences among P for fresh fruit bunch （FFB） yield, bunch number （BN） and average bunch weight （ABW） in Kluang, Ulu Paka and Teluk Intan, but not in Carey Island. FFB yield was generally higher on coastal soils （Teluk Intan and Carey Island） than inland soils （Kluang and Ulu Paka）. Heritability was calculated based on the intraclass correlation. Heritability estimates for these three yield components were variable, depending on the breeding material and environment in which the materials were tested. Fruit to bunch （F/B） and oil to bunch （O/B） of parthenocarpic fruits were important in determining the overall O/B of the interspecific hybrids. The O x P hybrids in Kluang showed the lowest height increment with only a mean of 14.0 cm/year, whereas in Ulu Paka and Teluk Intan, the values were higher at 24.0 cm/year and 25.0 cm/year, respectively. The study showed that the FFB yields ofoil palm interspecific hybrids performed better in coastal soils than inland soils.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.41176135,41206142)the Innovation Program of Chinese Academy of Sciences(No.KSCX2-EW-B-3)the Ministry of Agriculture of China(No.200903030)
文摘The annual life cycle of the brown seaweed Undariapinnatifida （Harvey） Suringer comprises a macroscopic diploid sporophyte stage and a microscopic haploid gametophyte stage. In 2011, an unusual zoospore-derived monoecious gametophyte isolate （designated as line 10-5-3） of U. pinnatifida was observed. To understand this phenomenon, a comprehensive screening of eighty-two previously identified male gametophyte cultures, isolated from three randomly selected cultivars （lines 10, 7, and 5） was performed. Thirty-six of the isolates developed both antheridia and oogonia on the same filamentous fragment in a standard gametogenesis test （SGT： 18℃, 60 umol photons/（m2.s））. Selfing of the monoecious gametophyte or crossing it with a normal male gametophyte both gave rise to morphologically normal sporophytic offspring. However, crossing resulted in a much higher fertilization rate （89.7%）. The hybrid and selfed sporophytic offspring were grown to maturity in flow tanks at an ambient temperature of 10-18℃ over a period of 69 days. Active zoospores were released from both types of mature sporophylls. The majority of these developed into male gametophytes, while 15%-20% developed into the observed monoecious structures on the same filament. Using PCR amplification it was found that all the monoecious gametophyte isolates and the sporophytic offspring resulting from the selfing and crossing lacked the femalelinked microsatellite sequence （a part of the locus Up-AC-2A8, GenBank accession No. AY738602.1）, indicating their male nature. U.pinnatifida is an invasive species in some regions and the implications of the above findings for this species in nature are briefly discussed.
文摘Progeny testing of oil palm interspecific hybrids was conducted in four trials in Kluang, Ulu Paka, Teluk Intan and Carey Island, Malaysia. The interspecific hybrids （O × P） were created according to the North Carolina Model I （NCM I） mating design, using Elaeis oleifera （0） and Elaeis guineensis var. pisifera （P） as the maternal and paternal parents, respectively. Differences among O, P and O-within-P were determined by the performance （bunch yield, components and vegetative traits） of the progenies. There were significant differences among P for fresh fruit bunch （FFB） yield, bunch number （BN） and average bunch weight （ABW） in Kluang, Ulu Paka and Teluk Intan, but not in Carey Island. FFB yield was generally higher on coastal soils （Teluk Intan and Carey Island） than inland soils （Kluang and Ulu Paka）. Heritability was calculated based on the intraclass correlation. Heritability estimates for these three yield components were variable, depending on the breeding material and environment in which the materials were tested. Fruit to bunch （F/B） and oil to bunch （O/B） of parthenocarpic fruits were important in determining the overall O/B of the interspecific hybrids. The O x P hybrids in Kluang showed the lowest height increment with only a mean of 14.0 cm/year, whereas in Ulu Paka and Teluk Intan, the values were higher at 24.0 cm/year and 25.0 cm/year, respectively. The study showed that the FFB yields ofoil palm interspecific hybrids performed better in coastal soils than inland soils.
