Objective： To study the effect of the extract of Prunella vulgaris L. on multiple drugs resistant bacillus tuberculosis （MDR-TB）. Methods： Experimental animal model in rats was induced by MDR-TB. Normal group mode...Objective： To study the effect of the extract of Prunella vulgaris L. on multiple drugs resistant bacillus tuberculosis （MDR-TB）. Methods： Experimental animal model in rats was induced by MDR-TB. Normal group model group and Prunella vulgaris L. group were set up. The contents of IFN-7, IL-4, IL-10 and IL-12 were examined by ELISA. Their genome mRNAs were extracted, the target genes were amplified by PCR. RT-PCR was used to detect the mRNA levels of them. Results： The content of IFN-q, of the extract of Prunella vulgaris L. group was 1.98±0.67 pg/ml, IL-4 was 6.47±1.46 pg/ml, IL-10 was 12.13±3.43 pg/ml and IL-12 was 3.02±0.86 pg/ml. Compared with the model group, Prunella vulgaris L. group was notable difference in serum IFN-γ, IL-12 and IL-10 （P〈0.05）. The mRNA levels of IFN-γ, IL-12 increased and IL-10 decreased obviously, the differences were quite significant （P〈0.05）, but IL-4 had no obvious change. Conclusion： The extract of Prunella vulgaris L. can enhance the cellar immunological function in rats from up-regulation of the level of genetic transcription, accordingly provide the theory basis of healing of tuberculosis with it.展开更多
Objective:The aim of this study was to observe the effect of the Prunella vulgaris L extract on the Jurkat human T lymphoma cell line.Methods:Jurkat cells were cultivated with different concentrations of the extract f...Objective:The aim of this study was to observe the effect of the Prunella vulgaris L extract on the Jurkat human T lymphoma cell line.Methods:Jurkat cells were cultivated with different concentrations of the extract from Prunella vulgaris L.The MTT assay and flow cytometry were employed to determine the cells' proliferation inhibition ratio and the apoptosis rates,respectively.Agarose gel electrophoresis was used to observe cellular DNA fragmentation,and western blotting was used to observe changes in Bcl-2 and Bax protein expression.Results:The Prunella vulgaris L extract remarkably inhibited the proliferation of Jurkat cells.This inhibition exhibited dose dependence,with an IC50 of 20.23 ± 0.31 μg/mL.Agarose gel electrophoresis showed that the apoptosis strap became wider and brighter,and flow cytometry showed that the apoptosis rate increased in a concentration-dependent manner.Western blotting showed that Bcl-2 protein was down-regulated and Bax protein was up-regulated during apoptosis.Conclusion:The extract from Prunella vulgaris L induced apoptosis of Jurkat cells by down-regulating Bcl-2 protein and up-regulating Bax protein.These actions inhibited the growth of Jurkat cells.展开更多
Objective Pelvic inflammatory disease(PID)is one of the most common gynaecological diseases.Here,this thesis aims to investigate the therapeutic effects of Prunella vulgaris L.oil on the PID by using metabolomics base...Objective Pelvic inflammatory disease(PID)is one of the most common gynaecological diseases.Here,this thesis aims to investigate the therapeutic effects of Prunella vulgaris L.oil on the PID by using metabolomics based on gas chromatographymass spectrometry(GC-MS)to address this challenge.Methods First,measurements of pro-inflammatory cytokines and histological analysis of the uterus were conducted to validate the successful generation of a PID rat model.Furthermore,the volatile oil from Prunella vulgaris L.was administered to treat PID rats.Serum samples were collected before and after treatment and analyzed by GC-MS to generate metabolite profiles for each sample.The information generated from the qualitative and quantitative analysis of these metabolites was applied to distinguish between the PID model and normal control groups.Results Some metabolites,such as acetic acid,succinic acid,glyceric acid,(R*,S*)-3,4-dihydroxybutanoic acid,3-hydroxyphenylacetic acid,D-ribose and myo-inositol showed a higher contribution in the classification model;thus,they can be considered as potential biomarkers.Furthermore,the therapeutic effect of the volatile oil extracted from Prunella vulgaris L.could also be visualized using GC-MS-based metabolomics.Conclusions The results show that metabolomics studies are invaluable for disease diagnosis and therapeutic effect estimation.展开更多
Floral traits and the number of visitors are expected to change with different mating systems.We tested this hypothesis by comparing flowers of Prunella vulgaris(Lamiaceae)with inserted and exserted styles across a st...Floral traits and the number of visitors are expected to change with different mating systems.We tested this hypothesis by comparing flowers of Prunella vulgaris(Lamiaceae)with inserted and exserted styles across a strongly exserted style biased,an exserted style biased,and a strongly inserted style biased subalpine population.We examined flowering phenology,floral morphology,flower visitation rate,capacity for autonomous selfpollination and visitor contribution to seed production for each style type and in each population.We also examined inbreeding depression(ID)by comparing the relative performance of progeny from self-and crosspollination.Exserted style plants had larger and more open flowers,increased pollen production,higher amounts of nectar rewards and higher visitation and outcrossing rates than inserted style plants.Similarly on the population level,the visitation rates were higher in the exserted style-biased populations than in the inserted style-biased population.Inserted style plants provided a stronger reproductive assurance(RA)through autonomous selfing than exserted style plants.RA and outcrossing rates did not differ among populations,showing low visitation rates may be sufficient for adequate seed production in P.vulgaris.Although inserted style plants had a lower ID level than exserted style plants,the ID of both was less than 0.5,suggesting that an ID should not counteract the evolution of selfing in this species.Inserted style plants provide RA through autonomous selfing,and exserted style plants ensure outcrossing through pollinator services,supporting a stable mixed mating system in this subalpine plant.展开更多
基金Supported by the Natural Science Foundation for Universities in Anhui Province (KJ2010A087 and KJ2008A152)
文摘Objective： To study the effect of the extract of Prunella vulgaris L. on multiple drugs resistant bacillus tuberculosis （MDR-TB）. Methods： Experimental animal model in rats was induced by MDR-TB. Normal group model group and Prunella vulgaris L. group were set up. The contents of IFN-7, IL-4, IL-10 and IL-12 were examined by ELISA. Their genome mRNAs were extracted, the target genes were amplified by PCR. RT-PCR was used to detect the mRNA levels of them. Results： The content of IFN-q, of the extract of Prunella vulgaris L. group was 1.98±0.67 pg/ml, IL-4 was 6.47±1.46 pg/ml, IL-10 was 12.13±3.43 pg/ml and IL-12 was 3.02±0.86 pg/ml. Compared with the model group, Prunella vulgaris L. group was notable difference in serum IFN-γ, IL-12 and IL-10 （P〈0.05）. The mRNA levels of IFN-γ, IL-12 increased and IL-10 decreased obviously, the differences were quite significant （P〈0.05）, but IL-4 had no obvious change. Conclusion： The extract of Prunella vulgaris L. can enhance the cellar immunological function in rats from up-regulation of the level of genetic transcription, accordingly provide the theory basis of healing of tuberculosis with it.
文摘Objective:The aim of this study was to observe the effect of the Prunella vulgaris L extract on the Jurkat human T lymphoma cell line.Methods:Jurkat cells were cultivated with different concentrations of the extract from Prunella vulgaris L.The MTT assay and flow cytometry were employed to determine the cells' proliferation inhibition ratio and the apoptosis rates,respectively.Agarose gel electrophoresis was used to observe cellular DNA fragmentation,and western blotting was used to observe changes in Bcl-2 and Bax protein expression.Results:The Prunella vulgaris L extract remarkably inhibited the proliferation of Jurkat cells.This inhibition exhibited dose dependence,with an IC50 of 20.23 ± 0.31 μg/mL.Agarose gel electrophoresis showed that the apoptosis strap became wider and brighter,and flow cytometry showed that the apoptosis rate increased in a concentration-dependent manner.Western blotting showed that Bcl-2 protein was down-regulated and Bax protein was up-regulated during apoptosis.Conclusion:The extract from Prunella vulgaris L induced apoptosis of Jurkat cells by down-regulating Bcl-2 protein and up-regulating Bax protein.These actions inhibited the growth of Jurkat cells.
基金We thank for the funding support from the National Natural Science Foundation of China(No.81503041)Natural Science Foundation of Hunan Province(No.2017JJ4045)Changsha Science and Technology Project(No.kq1701073).
文摘Objective Pelvic inflammatory disease(PID)is one of the most common gynaecological diseases.Here,this thesis aims to investigate the therapeutic effects of Prunella vulgaris L.oil on the PID by using metabolomics based on gas chromatographymass spectrometry(GC-MS)to address this challenge.Methods First,measurements of pro-inflammatory cytokines and histological analysis of the uterus were conducted to validate the successful generation of a PID rat model.Furthermore,the volatile oil from Prunella vulgaris L.was administered to treat PID rats.Serum samples were collected before and after treatment and analyzed by GC-MS to generate metabolite profiles for each sample.The information generated from the qualitative and quantitative analysis of these metabolites was applied to distinguish between the PID model and normal control groups.Results Some metabolites,such as acetic acid,succinic acid,glyceric acid,(R*,S*)-3,4-dihydroxybutanoic acid,3-hydroxyphenylacetic acid,D-ribose and myo-inositol showed a higher contribution in the classification model;thus,they can be considered as potential biomarkers.Furthermore,the therapeutic effect of the volatile oil extracted from Prunella vulgaris L.could also be visualized using GC-MS-based metabolomics.Conclusions The results show that metabolomics studies are invaluable for disease diagnosis and therapeutic effect estimation.
基金supported by the Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0502)the National Natural Science Foundation of China(31570385)Postdoctoral Fellowship of Xishuangbanna Tropical Botanical Garden,CAS and China Postdoctoral Science Foundation Funding(2019M653513).
文摘Floral traits and the number of visitors are expected to change with different mating systems.We tested this hypothesis by comparing flowers of Prunella vulgaris(Lamiaceae)with inserted and exserted styles across a strongly exserted style biased,an exserted style biased,and a strongly inserted style biased subalpine population.We examined flowering phenology,floral morphology,flower visitation rate,capacity for autonomous selfpollination and visitor contribution to seed production for each style type and in each population.We also examined inbreeding depression(ID)by comparing the relative performance of progeny from self-and crosspollination.Exserted style plants had larger and more open flowers,increased pollen production,higher amounts of nectar rewards and higher visitation and outcrossing rates than inserted style plants.Similarly on the population level,the visitation rates were higher in the exserted style-biased populations than in the inserted style-biased population.Inserted style plants provided a stronger reproductive assurance(RA)through autonomous selfing than exserted style plants.RA and outcrossing rates did not differ among populations,showing low visitation rates may be sufficient for adequate seed production in P.vulgaris.Although inserted style plants had a lower ID level than exserted style plants,the ID of both was less than 0.5,suggesting that an ID should not counteract the evolution of selfing in this species.Inserted style plants provide RA through autonomous selfing,and exserted style plants ensure outcrossing through pollinator services,supporting a stable mixed mating system in this subalpine plant.
