Matching Dyeing and Properties of Silk Fabrics with Natural Edible Pigments

The silk fabrics were matching dyed with three natural edible pigments(red rice red,ginger yellow and gardenia blue).By investigating the dyeing rates and lifting properties of these pigments,it was observed that their compatibilities were excellent in the dyeing process:dye dosage 2.5%(omf),mordant alum dosage 2.0%(omf),dyeing temperature 80℃and dyeing time 40 min.The silk fabrics dyed with secondary colors exhibited vibrant and vivid color owing to the remarkable lightness and chroma of ginger yellow.However,gardenia blue exhibited multiple absorption peaks in the visible light range,resulting in significantly lower lightness and chroma for the silk fabrics dyed with tertiary colors,thus making it suitable only for matte-colored fabrics with low chroma levels.In addition,the silk fabrics dyed with these three pigments had a color fastness that exceeded grade 3 in resistance to perspiration,soap washing and light exposure,indicating acceptable wearing properties.The dyeing process described in this research exhibited a wide range of potential applications in matching dyeing of protein-based textiles with natural colorants.

天色与脸色

广东话“出门睇天色，入门睇脸色”是一种人生的哲学。

Breeding of a Natural Colored-Cocoon Bombyx mori Variety Shuhuang No.1

[Objective] This study aimed to develop a new Bombyx mori variety Shuhuang No.1 and investigate its characteristics.[Method] The new variety was developed through cross breeding and pedigree separation.Its characteristics were identified by laboratory tests and rural promotion practices in Sichuan Province.[Result]The cocoon produced by the new variety Shuhuang No.1 was golden-yellow and radiantly beautiful.Shuhuang No.1 was strong and easy to rear,uniform in development.The male and female ones could be distinguished by their larval color and markings.The cocoon yield per 10 000 the 4th instar larvae reached 20.30 kg; the length of a cocoon filament was about 1 115.0 m,and a non-broken filament was 882.95 mm long,accounting for 79.15％ of the total length.The raw silk ratio of fresh cocoon was 18.39％,and the neatness was 91.25 point.The main economic features of Shuhuang No.1 were better than those of the control.[Conclusion] The new variety had been approved by Sichuan Silkworm Evaluation Commission.It can be promoted in parent silkworm rearing areas Sichuan,Chongqing,Yangtze River basin.

Discussion on Green Development of Fenlong for Yield Increase, Quality Enhancing, Water Retaining and Multiple Use of Natural Resources

Fenlong green ecological agriculture technology （Fenlong technology）, a new smash ridging farming method developed by Guangxi Academy of Agdcultural Sciences, has been elected as the recommended cultivation technique by the Ministry of Agriculture of China. It replaces the traditional plowshare with spiral drill, and its tilth depth is twice deeper than that by tractor tilthing. It also extends soil nutrient, moisture, oxygen and microorganism, the so-called ＂Four pools＂. Soil nutrient, oxygen, microorganism, light and rainfall use ratio is increased by 10%-100%, creating a platform for natural increase of more than 10% of crop yield. Its application to over 20 kinds of crops in 21 provinces has proved that the yield increases 10-30% with quality enhancing 5% and double water retaining capacity but no more input. When the application area of Fenlong could reach 67 million hm2, the amount of fertilizer can be reduced by 40-50 billion kg, saving 120-150 billion Yuan. In this paper, we put forward the strategy of ＂4＋1＂ （arable, saline-alkali soil, grasslands, Sponge City ＋ rivers） green development in China, and deepened the Fenlong cultivated tilled layer from 16.5 cm to 35 cm for 67 million hm2 arable land, ridged 13.3 million hm2 of saline-alkali soil for 35 cm, and also 35 cm for 67 million hm2 degraded steppe, which could have the following 3 effects： first, the 147 million hm2 of land with Fenlong cultivation could increase loosing soil to 315.491 billion m3, in＊ creasing by 159.26% for 120 million hm2 of arable land with the average tilled layer of 16.5 cm, which has loosing soil of only 198.1 billion m3, that is, the space of the land increases 1.6 times. Second, every hectare of plowland could store up to 450 m3/hm2 of natural rainfall, and the unused 60 m3 of saline-alkali soil and grasslands could store water of 102 billion m3, showing an increase of over 88.89% for the current plowland storage of 54 billion m3 at now, that is, double the natural rainfall storage capacity. Third, the two multiple increase of natural resources application can bring trillions of resource activation, environmental cleaning, food security, citizens, health, economic, ecological and social benefits, and makes the Chinese nation move forward in green development. Its application in ＂big scientific research＂ and ＂One Belt And One Road＂ will contribute Chinese strength to the world.

Repeated-batch Cultivation of Encapsulated Monascus purpureus by Polyelectrolyte Complex for Natural Pigment Production

In general,productions of natural pigment in submerged microorganism culture were much less than that in solid-state fermentation,because the solid-state culture can provide a support carrier for the mycelium. To improve natural pigment production,the cultivation of Monascus purpureus in submerged encapsulated cell was investigated. Monascus purpureus immobilized in polyelectrolyte complex(PEC) microcapsules,which were pre-pared by sodium cellulose sulphate(NaCS) and poly-dimethyl-diallyl-ammonium chloride(PDMDAAC),was a good substitute for submerged cell culture because it mimicked the solid-state environment. The repeated-batch process with encapsulated cells was studied in flasks and a bubble column. The results indicated that the bubble column was more suitable for the encapsulation culture than the shaking flasks because of its good mass transfer performance and minor shear stress on cells. Owing to the protection of the microcapsule's membrane,Monascus purpureus in microcapsules increased approximately three times over that in free cell culture with negligible cell leakage to the medium. The pigment production in the bubble column finally reached 3.82(OD500) ,which was two times higher than in free cell culture. In addition,the duration of each batch was shortened to 15% of that in free cell culture.

Charge coupled devices （CCD） photometry and long-term optical variability of gamma-ray-loud BL Lacertae object Mkn 421

Evidence for the long-term optical curve variability and colour behaviour of the BL Lac object Mkn 421 is presented. Our results show that the amplitude of the optical variations of Mkn 421 is only about △B=4.7 magnitude in the B-band for its photometric history from 1899 to 2002. The results of optical photometric monitoring of the Mkn 421 from April 2000 to Jan 2002 are provided. During our observation, Mkn 421 shows significant rapid variations and exhibits short time variability of 2.42 hours in the B band. A strong correlation between the B - V colour index and the magnitude in the B band is found. Our charge coupled device （CCD） photometry of Mkn 421 shows that the measured results are in good agreement with the predicted optical variability period of about 23 years.

Study on the Extracting Technology of Natural Lycopene

Optimum technical parameters for extracting lycopene from tomatoes are studied with orthogonal experiments.The results show that chloroform has the best effect compared to 4 other groups of solvents:petroleum ether,acetic add + ethanol,acetic ether,and acetone + diethyl ether,associated with the parameters of 40℃,pH 5 and the ratio of tomato paste to solvent 1:1.

Variation in metabolic enzymatic activity in white muscle and liver of blue tilapia,Oreochromis aureus,in response to long-term thermal acclimatization

The effects of rearing temperature on white muscle and hepatic phosphofructokinase （PFK）, pyruvate kinase （PK）, lactate dehydrogenase （LDH）, aspartate aminotransferase （AST） and alanine aminotransferase （ALT） were examined in fingerlings of blue tilapia, Oreochromis aureus. The experiment was conducted for 14 weeks at temperatures of 18, 22, 26, 30, and 34℃. The activity of the glycolytic enzymes PFK, PK, and LDH in white muscle increased significantly with increase in water temperature. A reverse trend was observed for these enzymes in the liver, except for LDH, which behaved in the same manner as in white muscle. Cytosolic AST and ALT activity increased in both white muscle and liver in response to warm thermal acclimatization, while a reduction in mitochondrial AST and ALT activity was noticed at high temperatures in comparison with those at a lower temperature.

Abiotechnology of Indigofera tinctoria L. on the Saline Land of Aral Sea Basin and Producing of the Natural Plant Indigo Pigment for the Industry

The possibility of cultivation of lndigofera tinctoria L. plants in ecologically degraded and saline lands of the Aral Sea Basin was studied. A new salt-resistant Indigofera plant variety ＂Feruz-l＂ was producing by the classic methods of genetics and modem biotechnology.Agro-technology of cultivation as main and secondary culture after wheat was developed and recommended to the farmers of the country.The biotechnology of natural plant pigment indigo extraction has been developed and was recommended for using in different fields of industry： textile, pharmacy-pharmacology, perfume-cosmetic, architectural-decorative and food industry. Quantitative and qualitative HPLC （high pressure liquid chromatography） method of determination of plant natural indigo has been also developed.

《史记·孝文本纪》误(失)注词句辨析

对于《史记.孝文本记》中的某些词句,注家昧于古义僻义,而以今义常义解之;更有不明《史记》"鱼藻"之义和"互见"史法,对有些词句如"廪廪乡改正服封禅矣,谦让未成于今"等附会曲解,以致文义扦格难通,出现许多讹误。本文选择几条加以辨析。

Adsorption and Step Elution of Urokinase Using Affinity Chromatography-Comparison of Data with Rate Model Simulation

A non-equilibrium chromatographic rate model was employed to simulate the affinity chromatography of urokinase. The chromatography process was developed to a yield of high purity product of urokinase from crude materials. The affinity gel used in the process was prepared by an epichlorohydrin-activation method using epichlorohydrin activated Sepharose 4B as a matrix and p-aminobenzamidine as a ligand. The chromatographic process were numerically simulated and analyzed with the aid of VERSE-LC computer simulator. Considering the basic principles, rate model with the back mixing in column inlet was utilized in simulating and studying the effect of the column inlet pattern on other parameters. Comparison of the simulation results with the experimental data showed that the rate model can be used to describe the affinity chromatography of urokinase in a fixed bed column with satisfactory accuracy.

Effect of Natural Surface Secretes of Some Common Ornamental Plants Leaves on Pathogenic Micro-organisms

This study presents the antimicrobial effect of natural surface secretes of some common ornamental plants leaves （Ficus elastica, Philodendrom bipinnatifidum and Aglonema） against some pathogenic bacteria （ Escherichia coli, P. aeruginosa and Staph. Aureus）, and fungi （Microsporum gypseum and Aspergillus flavus）. It was concluded that all tested washing water of the plants leaves secretes exhibited various inhibitory effects, both Philodendrom more antibacterial activity than Ficus elastica. While both E. coli and P bipinnatifidum and Aglonema commutatum had exhibited aeruginosa were being more sensitive than Staph. aureus. The same inhibitory effects were observed when the plants leaves inoculated in their surface with pathogenic bacteria. In contrast to bacterial inhibitory effects, the washing water of natural surface secretes of tested ornamental plants leaves induced mycelium growth of both tested fungi. Microsporum gypseum mycelium growth induced more than Aspergillusflavus especially in case of the washing water of Ficus elastic that have highest effects at 7.5/500 mL of medium. This study concluded the uses of ornamental plants for the indoors and outdoors to control the growth of pathogenic microbes and problems associated with hospital.

Improvement in Oxidative Stability of Soybean Oil by Mango Kernel Extracts

This work was undertaken to explore the potential of fruit waste materials as sources of natural antioxidants. Kernels of four varieties of mangoes of Indian origin were studied. Kernels were extracted with five different solvents, tested for extraction efficiency and total phenolic content （TPC）. As the methanol proved to be the best solvent, it was used for further analysis. The methanolic extracts of kernels had reducing power （RP） and scavenged 2, 2 diphenyl-l-picryl hydrazyl （DPPH） radical, which is related to their antioxidant activity （AA）. When analyzed in HPLC, the extracts showed the presence of phenolic compounds. The extracts had the higher capacity to reduce the formation of peroxides and slow down the rate of oxidation than butylated hydroxyl toluene （BHT） in refined, bleached deodorized soybean oil （RBD SBO）. Based on the results obtained mango kernels are potential source of natural antioxidants owing to their antioxidant activity.

Molecularly Imprinted Polymers for Selective Extraction of Crystal Violet from Natural Seawater coupled with HighPerformance Liquid Chromatographic Determination

Molecularly imprinted polymers （MIPs） were prepared by the bulk polymerization using crystal violet as the template molecule, and the methacrylic acid and ethylene glycol dimetheacrylate as functional monomer and cross-linker, respectively. Sys- tematic investigations of synthetic conditions were conducted. The surface morphology and recognition mechanism of the obtained polymers were studied using scanning electron microscope and spectrophotometric analysis. MIPs showed high atTmity to template molecule and were successfully applied as special solid-phase extraction sorbent for selective extraction of crystal violet from natural seawater. An off-line molecularly imprinted solid-phase extraction （MISPE） method followed by high-performance liquid chroma- tography with diodearray detection for the analysis of crystal violet was also established. MISPE columns have good recoveries for crystal violet standard solutions and good linearity was obtained over the concentration range of 0-200 ~tg L-1 （R2 〉 0.99）. Finally, two natural seawater samples were investigated. The recoveries of spiked seawater on the MISPE columns were from 44.47% to 62.34%, the relative standard deviation （n=3） being in the range of 2.89%-5.96%.

Assessing the kinetic model of hydro-distillation and chemical composition ofAquilaria malaccensis leaves essential oil

This study aimed to model the kinetic of hydro-distillation of Aquilaria malaccensis leaves oil in order to understand and optimize the extraction process. In addition, this study, for the first time, aimed to identify the chemical compositions of the A. rnalaccensis leave-oil. By assessing both first-order kinetic model and the model of simultaneous washing and diffusion, the result indicated that the model of simultaneous washing and diffusion better describes the hydro-distillation mechanism of the essential oil from A. rnalaccensis leaves. The optimum time, solid to liquid ratio, and the heating power for extracting the highest amount of essential oil were found to be around 3 h, 1：10 （g. ml-1）, and 300 W respectively. Yellow essential oil with a strong smell and a yield of 0.05 v/w was extracted by hydro-distillation Clevenger apparatus. Chemical compounds of the essential oil were analyzed using gas chromatography-mass spectroscopy （GC/MS）, which resulted in identification of 42 compounds that constitute 93% of essential oil. Among the identified components, Pentadecanal （32.082%）, 9-Octadecenal, （Z） （15.894%）, and Tetradecanal （6.927%） were the major compounds. Considering the fact that all the identified major components possess pesticidal properties, A. malaccensis leaves can be regarded as a promising natural source for producing pesticides.

Preparation of National CRMs of Active Compounds from Natural Products

According to the standard of GB/T1500.3-2008 and the guidelines of ISO/REMCO, the preparation of National Certified Reference Materials （CRMs） of active compounds was composed of the following two aspects： preparation and certification. This paper presents an example of the preparation of biochanin A to illustrate the preparation of National CRMs of active compounds from natural products. Firstly, the target compound was extracted and purified from crude materials. Then, its chemical structure was identified by UV spectrum, IR spectrum, ESI-MS, and NMR. The purity of the compound was determined by HPLC with the area normalization method. The certified value assessed by comparing the purity values from six independent qualified laboratories and the value of uncertainty were given. Thermal analysis, residual solvent, homogeneity testing and stability testing was tested in the process.

天色

一条百里长的大鱼，在盆地的锅里煎炸，渐渐地，排列整齐的银白色鳞片红了，桃色的红，金色的红，一仰望便眼花缭乱。太阳在另一面添柴，想必整条鱼就要熟透了。

天色晴好上王山

天蓝云淡翻高岭，好景深藏涧水鸣。勇往巅峰不畏险，欢歌绕树逶迤行。远途练就豪杰骨，近水生成绕指情。团结互帮内心暖，专家摄影更欢迎。

Impact of otrA expression on morphological differentiation,actinorhodin production,and resistance to aminoglycosides in Streptomyces coelicolor M145

otrA resembles elongation factor G （EF-G） and is considered to be an oxytetracycline （OTC）-resistance determinant in Streptomyces rimosus. In order to determine whether otrA also conferred resistance to OTC and other aminoglycosides to Streptomyces coelicolor, the otrA gene from S. rimosus M527 was cloned under the control of the strong ermE promoter. The resulting plasmid, pIB139-otrA, was introduced into S. coeficolor M145 by intergenedc conjugation, yielding the recombinant strain S. coelicolor M145-OA. As expected S. coelicolor M145-OA exhibited higher resistance levels specifically to OTC and aminoglycosides gentamycin, hygromycin, streptomycin, and spectinomycin. However, unexpectedly, S. coelicolor M14-~OA on solid medium showed an accelerated aerial mycelia formation, a precocious sporulation, and an enhanced actinorhodin （Act） production. Upon growth in 5-L fermentor, the amount of intra- and extracellular Act production was 6-fold and 2-fold higher, respectively, than that of the original strain. Consistently, reverse transcription polymerase chain reaction （RT-PCR） analysis revealed that the transcrip- tional level of pathway-specific regulatory gene acUl-orf4 was significantly enhanced in S. coelicolor M145-OA compared with in S. coelicolor M145.

Separation of injectable salidroside by column chromatography of macroporous resins for treating myocardial ischemia

The objective of the present study is to develop a method for large-scale separating and purifying salidroside from rhodiola kirilowii roots and for preparing injectable medicinal ingredient.Crude extract of salidroside was prepared by water-ethanol system,and purified by column chromatography of macroporous resins.Static adsorption and desorption studies were performed on six kinds of macroporous resins,and SP825 resin was chosen,followed by optimizing process parameters.The optimum sample volume,feed concentration,ratio of diameter to height,and feeding flow rate were 1.5 bed volumes(BV),15 mg/mL,1:10 and 1 BV/h,respectively.Dynamic desorption was performed consecutively with 8 BV of distilled water,3 BV of 5% ethanol and 8 BV of 10% ethanol at a flow rate of 2 BV/h.After three cycles in separating 3.5 tons of rhodiola kirilowii roots,salidroside purity was increased from 3.4% in the crude extract to 93.6% in purified salidroside product.This study provides a novel method to separate salidroside for injectable use.