A new approach for rules-based optical proximity correction is presented.The discussion addresses on how to select and construct more concise and practical rules-base as well as how to apply that rules-base.Based on t...A new approach for rules-based optical proximity correction is presented.The discussion addresses on how to select and construct more concise and practical rules-base as well as how to apply that rules-base.Based on those ideas,several primary rules are suggested.The v-support vector regression method is used to generate a mathematical expression according to rule data.It enables to make correction according to any given rules parameters.Experimental results demonstrate applying rules calculated from the expression match well with that from the rule table.展开更多
Objective To genetically correct a disease-causing point mutation in human induced pluripotent stem cells （iPSCs） derived from a hemophilia B patient. Methods First, the disease-causing mutation was detected by ...Objective To genetically correct a disease-causing point mutation in human induced pluripotent stem cells （iPSCs） derived from a hemophilia B patient. Methods First, the disease-causing mutation was detected by sequencing the encoding area of human coagulation factor IX （F IX） gene. Genomic DNA was extracted from the iPSCs, and the primers were designed to amplify the eight exons of F IX. Next, the point mutation in those iPSCs was genetically corrected using CRISPR/Cas9 technology in the presence of a 129-nucleotide homologous repair template that contained two synonymous mutations. Then, top 8 potential off-target sites were subsequently analyzed using Sanger sequencing. Finally, the corrected clones were differentiated into hepatocyte-like cells, and the secretion of F IX was validated by immunocytochemistry and ELISA assay.Results The cell line bore a missense mutation in the 6th coding exon （c.676 C〉T） of F IX gene. Correction of the point mutation was achieved via CRISPR/Cas9 technology in situ with a high efficacy at about 22% （10/45） and no off-target effects detected in the corrected iPSC clones. F IX secretion, which was further visualized by immunocytochemistry and quantified by ELISA in vitro, reached about 6 ng/ml on day 21 of differentiation procedure. Conclusions Mutations in human disease-specific iPSCs could be precisely corrected by CRISPR/Cas9 technology, and corrected cells still maintained hepatic differentiation capability. Our findings might throw a light on iPSC-based personalized therapies in the clinical application, especially for hemophilia B.展开更多
English learning and teaching has been a hot research topic in China since the coming of the 21 st century. By way of questionnaire surveys and interviews, the research group takes one ordinary Chinese university as a...English learning and teaching has been a hot research topic in China since the coming of the 21 st century. By way of questionnaire surveys and interviews, the research group takes one ordinary Chinese university as a case to study the reform of College English curriculum setup. The paper focuses on study of extended courses, which are the most typical reflections of the reform in this university. In the first stage, 4,330 students are surveyed or interviewed in the pre-research process and another 1,270 participants from Grades 2013 and 2014 in the second stage fmish the questionnaire about the reform. The paper analyses the results of 1,215 valid questionnaires. The analyses and discussions in the research include four aspects, which are participants＇ overall attitudes towards the reform of College English curriculum setup, current situation of course teaching and assessment, degree of satisfaction with the extended courses and participants＇ feedbacks and suggestions. In the end of the study the existing problems in the reform are listed and suggestions are given to further reform the College English curriculum.展开更多
In the present paper, a comparison is made of the three formats in the history of EECU （English education in China＇s universities）. In the first period （1904-1949）, English education was conducted separately in g...In the present paper, a comparison is made of the three formats in the history of EECU （English education in China＇s universities）. In the first period （1904-1949）, English education was conducted separately in government-managed educational institutions and in institutions sponsored by church-related organizations; English education was well-knit with both general education and specialty education; and self-motivating learning was encouraged. In the second period （1950-1966）, almost everything was tinted with a shady color of politics： A halt was addressed drastically to EEC （English education in China） in 1952. In the gradual recovery that started four years later, the second format was sawed and hammered, showing the following features： All non-government-mamaged institutions vanished from the stage; English was taught solely as a language or a system of verbal parts, almost deprived of all cultural loading. The third period （1978 onwards） has witnessed a barrier-free and rapid development of over three decades, resulting in the unprecedented pervasion of EECU. Yet in the third tbrmat, learners＇ efforts have turned wholly test-oriented, degenerating into the saddening disintegrity of learning as a process into isolated charges to the target of a test at a time; the ignorant reduction of the learning methods to ＂Vocabulary Booklets Plus Collections of Test Papers＂. Such a comparison not only provides a multi-dimensional perspective of EECU and a better understanding of it, but also offers some important experiences and lessons for the search of an effective solution to the pervasive problem of＂Time-Consumingness and Low-Efficiency＂.展开更多
After perceiving a common phenomenon of English learning at college in China, this paper presents a case study of motivation. In this study, a questionnaire is conducted twice among 156 Chinese college students when t...After perceiving a common phenomenon of English learning at college in China, this paper presents a case study of motivation. In this study, a questionnaire is conducted twice among 156 Chinese college students when they are sophomores and seniors. It intends to identify some components of foreign language learning motivation and probes into the causes for the changing motivations of students throughout 4-year college education. The findings suggest that the instrumental motivation plays a crucial role in foreign language learning context.展开更多
文摘A new approach for rules-based optical proximity correction is presented.The discussion addresses on how to select and construct more concise and practical rules-base as well as how to apply that rules-base.Based on those ideas,several primary rules are suggested.The v-support vector regression method is used to generate a mathematical expression according to rule data.It enables to make correction according to any given rules parameters.Experimental results demonstrate applying rules calculated from the expression match well with that from the rule table.
基金Supported by the National Science and Technology Major Project(2011ZX09102-010-04)
文摘Objective To genetically correct a disease-causing point mutation in human induced pluripotent stem cells （iPSCs） derived from a hemophilia B patient. Methods First, the disease-causing mutation was detected by sequencing the encoding area of human coagulation factor IX （F IX） gene. Genomic DNA was extracted from the iPSCs, and the primers were designed to amplify the eight exons of F IX. Next, the point mutation in those iPSCs was genetically corrected using CRISPR/Cas9 technology in the presence of a 129-nucleotide homologous repair template that contained two synonymous mutations. Then, top 8 potential off-target sites were subsequently analyzed using Sanger sequencing. Finally, the corrected clones were differentiated into hepatocyte-like cells, and the secretion of F IX was validated by immunocytochemistry and ELISA assay.Results The cell line bore a missense mutation in the 6th coding exon （c.676 C〉T） of F IX gene. Correction of the point mutation was achieved via CRISPR/Cas9 technology in situ with a high efficacy at about 22% （10/45） and no off-target effects detected in the corrected iPSC clones. F IX secretion, which was further visualized by immunocytochemistry and quantified by ELISA in vitro, reached about 6 ng/ml on day 21 of differentiation procedure. Conclusions Mutations in human disease-specific iPSCs could be precisely corrected by CRISPR/Cas9 technology, and corrected cells still maintained hepatic differentiation capability. Our findings might throw a light on iPSC-based personalized therapies in the clinical application, especially for hemophilia B.
文摘English learning and teaching has been a hot research topic in China since the coming of the 21 st century. By way of questionnaire surveys and interviews, the research group takes one ordinary Chinese university as a case to study the reform of College English curriculum setup. The paper focuses on study of extended courses, which are the most typical reflections of the reform in this university. In the first stage, 4,330 students are surveyed or interviewed in the pre-research process and another 1,270 participants from Grades 2013 and 2014 in the second stage fmish the questionnaire about the reform. The paper analyses the results of 1,215 valid questionnaires. The analyses and discussions in the research include four aspects, which are participants＇ overall attitudes towards the reform of College English curriculum setup, current situation of course teaching and assessment, degree of satisfaction with the extended courses and participants＇ feedbacks and suggestions. In the end of the study the existing problems in the reform are listed and suggestions are given to further reform the College English curriculum.
文摘In the present paper, a comparison is made of the three formats in the history of EECU （English education in China＇s universities）. In the first period （1904-1949）, English education was conducted separately in government-managed educational institutions and in institutions sponsored by church-related organizations; English education was well-knit with both general education and specialty education; and self-motivating learning was encouraged. In the second period （1950-1966）, almost everything was tinted with a shady color of politics： A halt was addressed drastically to EEC （English education in China） in 1952. In the gradual recovery that started four years later, the second format was sawed and hammered, showing the following features： All non-government-mamaged institutions vanished from the stage; English was taught solely as a language or a system of verbal parts, almost deprived of all cultural loading. The third period （1978 onwards） has witnessed a barrier-free and rapid development of over three decades, resulting in the unprecedented pervasion of EECU. Yet in the third tbrmat, learners＇ efforts have turned wholly test-oriented, degenerating into the saddening disintegrity of learning as a process into isolated charges to the target of a test at a time; the ignorant reduction of the learning methods to ＂Vocabulary Booklets Plus Collections of Test Papers＂. Such a comparison not only provides a multi-dimensional perspective of EECU and a better understanding of it, but also offers some important experiences and lessons for the search of an effective solution to the pervasive problem of＂Time-Consumingness and Low-Efficiency＂.
文摘After perceiving a common phenomenon of English learning at college in China, this paper presents a case study of motivation. In this study, a questionnaire is conducted twice among 156 Chinese college students when they are sophomores and seniors. It intends to identify some components of foreign language learning motivation and probes into the causes for the changing motivations of students throughout 4-year college education. The findings suggest that the instrumental motivation plays a crucial role in foreign language learning context.
