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《实达》:沟通创造价值
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作者 周涧 《商界名家》 2004年第2期109-109,共1页
“每一天都是一个新的开始”——这是《实达》月刊封面刊头主题词,这平实的词语中透露着“实达”人永远向上的朝气与活力。
关键词 《实达》 福建实达电脑集团股份有限公司 内部资讯刊物 企业形象
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Selection of Reference Genes in Transcription Analysis of Gene Expression of the Mandarin Fish, Siniperca chuasti 被引量:17
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作者 周瑞雪 蒙涛 +6 位作者 孟海波 陈敦学 宾石玉 成嘉 符贵红 褚武英 张建社 《Zoological Research》 CAS CSCD 北大核心 2010年第2期141-146,共6页
At present, transcription analysis of gene expression commonly uses housekeeping genes as control for normalization. In this study, the expression levels of three housekeeping genes including GAPDH, β-actin, and 18S ... At present, transcription analysis of gene expression commonly uses housekeeping genes as control for normalization. In this study, the expression levels of three housekeeping genes including GAPDH, β-actin, and 18S rRNA in six tissues and five developmental stages of the Mandarin fish Siniperca chuatsi were assayed with quantitative real-time PCR (qPCR). Differences in expression levels were analyzed using geNorm program. The results demonstrate that β-actin is the most stable gene at developmental stages and GAPDH is the most stable in different tissues. While 18S rRNA expression during development is differentially regulated, which indicates it is suitable as an internal control for gene expression normalization at the developmental level. Overall, the data suggest that the two most stable housekeeping genes are enough to accurately calibrate gene expression in S. chuatsi. The significance of this study provided convincing references and methodology for housekeeping gene selection and normalization in gene expression analysis with regular PCR or qPCR. 展开更多
关键词 Reference genes geNorm program Gene expression Real-time PCR
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Overexpression of IGF2R and IGF1R mRNA in SCNT-produced Goats Survived to Adulthood 被引量:1
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作者 邢宝松 徐银学 +2 位作者 成勇 刘红林 杜淼 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2007年第8期709-719,共11页
The procedure of somatic cell nuclear transfer (SCNT) is likely to affect the expression level of growth-related genes especially imprinting genes. In this study, expressions of growth-related genes including three ... The procedure of somatic cell nuclear transfer (SCNT) is likely to affect the expression level of growth-related genes especially imprinting genes. In this study, expressions of growth-related genes including three imprinting genes (H19, IGF2, and IGF2R) and four non-imprinting genes (IGF1, IGFIR, GHR, and GHSR) in adult nuclear transferred (NT) goats were investigated by real-time PCR. The expressions of these genes in adult clones were found largely normal, but IGF2R and IGFIR were more highly expressed in cloned goats than in non-NT goats (P 〈 0.01). Analysis on mono-allelic expression pattern of imprinting genes indicated that mono-allelic expression patterns of H19 and IGF2 in cloned goats were similar to that in non-NT goats. In addition, the sequence of goat IGF2 gene and the putative amino acid sequence were obtained. The 986 nucleotide cDNA of goat IGF2 gene contained an open-reading frame of 540 nucleotides coding for 179 amino acids. Both cDNA sequence and amino acid sequence of IGF2 in goat showed their higher homology with that in sheep than in cattle; the partial cDNA fragments of H19, IGF2R, GHSR, IGFIR, and GHR in goat were also cloned and sequenced, which shared higher sequence identities with those in sheep than in cattle. 展开更多
关键词 GOAT nuclear transfer gene expression real-time PCR imprinting gene
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High resolution GPR and its experimental study 被引量:2
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作者 黄玲 曾昭发 +1 位作者 王牧男 王者江 《Applied Geophysics》 SCIE CSCD 2007年第4期301-307,共7页
We develop a high resolution ground penetrating radar system (LANRCS-GPR) based on the E5071B Vector Network Analyzer (VNA). This system takes advantage of a wideband and adjustable frequency domain ground penetra... We develop a high resolution ground penetrating radar system (LANRCS-GPR) based on the E5071B Vector Network Analyzer (VNA). This system takes advantage of a wideband and adjustable frequency domain ground penetrating radar system and adds the characteristics of a network analyzer with ultra-wideband and high precision measurement. It adopts the LAN mode to concatenate system control that reduces construction cost and makes the system easy to expand. The high resolution ground penetrating radar system carries out real time imaging using F-K migration with high calculation efficiency. The experiment results of the system indicate that the LANRCS-GPR system provides high resolution and precision, high signal-to-noise ratio, and great dynamic range. Furthermore, the LANRCS-GPR system is flexible and reliable to operate with easy to expand system functions. The research and development of the LANRCS-GPR provide the theoretical and experimental foundation for future frequency domain ground penetrating radar production and also can serve as an experimental platform with high data gathering precision, enormous information capability, wide application, and convenient operation for electromagnetic wave research and electromagnetic exploration. 展开更多
关键词 high resolution ground penetrating radar vector network analyzer and frequency domain
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Analysis of Gene Expression of Seven Isoforms of ADP-glucose Pyrophosphorylase in Rice Endosperm under Different Temperature Conditions
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作者 袁定阳 孙志忠 +1 位作者 谭炎宁 段美娟 《Agricultural Science & Technology》 CAS 2012年第6期1226-1229,1233,共5页
[Objective] This study aimed to analyze the effects of temperature on the expression of AGPase isoform genes in rice endosperm during milk stage. [Method] Different temperature treatments (33 and 25 ℃ of daily mean ... [Objective] This study aimed to analyze the effects of temperature on the expression of AGPase isoform genes in rice endosperm during milk stage. [Method] Different temperature treatments (33 and 25 ℃ of daily mean temperature for high and normal temperature treatments, respectively) and the real-time fluorescence quantitative PCR ( FQPCR) were used to analyze the expression patterns of seven isoforms (AGPS1, AGPS2a, AGPS2b, AGPL1, AGPL2, AGPL3 and AGPL4) of ADPglucose pyrophosphorylase (AGPase) which was the key enzyme in starch synthesis and metabolism in rice endosperm of two rice varieties Teqing and Thai Fragrant Rice. [Result] The AGPase isoforms AGPS2b, AGPL2 and AGPL3 had much higher expression than the other four isoforms, thus they were thought to be the main expression patterns of AGPase in rice endosperm. The relative expressions of AGPL2 was the highest among all the isoforms. The relative expressions of AGPS2b, AGPL2 and AGPL3 were higher in the normal temperature treatment than in the high temperature treatment in both rice varieties. The relative expression of the three enzyme genes in milk stages in Teqing was higher than those in Thai Fragrant Rice under different temperature treatments. [Conclusion] This study provides a theoretical basis for further use of molecular biology techniques to cultivate stable high-quality rice varieties. 展开更多
关键词 RICE ADP-glucose pyrophosphorylase (AGPase) isoforms Gene expression characteristics Real-time fluorescence quantitative PCR (FQ-PCR)
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Analysis of Seed-specificity of Silencing fad_2 Gene Expression in Transgenic Rapeseed Line W-4(Brassica napus L.) 被引量:3
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作者 陈松 彭琦 +5 位作者 周晓婴 高建芹 张维 张洁夫 浦惠明 戚存扣 《Agricultural Science & Technology》 CAS 2014年第8期1308-1311,1316,共5页
This study was to investigate the efficiency and specificity of RNAi silencing on the expression of endogenous fad2 gene in transgenic line W-4. [Method] The relative expression of fad2 gene in seeds at different deve... This study was to investigate the efficiency and specificity of RNAi silencing on the expression of endogenous fad2 gene in transgenic line W-4. [Method] The relative expression of fad2 gene in seeds at different developmental stages of 7th, 14th, 21st and 28th day after flowering (DAF) as wel as the root, stem, leaf at winter seedling stages of both the transgenic line W-4 and non-transgenic control Westar by real-time fluorescence quantitative PCR. [Results] The results showed the relative expression of fad2 gene was gradual y increasing with the days after flowering in the seeds of the control Westar, while it was found decreasing significantly since the 21st DAF in the seeds of the line W-4. The decline was up to 60% in comparison with the control Westar. However, no significant difference in the relative expression of fad2 gene in other organs like root, stem and leaf was observed between transgenic line W-4 and non-transgenic control Westar. Fatty acid composition analysis showed the oleic acid desaturation parameter(ODP) in seeds of the line W-4 was 0.07 in average, decreased by nearly 75% than control Westar which was 0.24 in average, while no significant difference in the seedling root, stem and leaf was measured between transgenic rapeseed and control. [Conclusion] The results above validated that RNA interference in transgenic rapeseed W-4 is at a seed-specific manner, not interfering with fad2 gene expression in organs such as the root, stem and leaf. The study also found that the period of fad2 gene expres-sion decline was wel coincided with the expression of napin gene, both appeared at the 21st DAF, indicating that the expression of dsRNA of fad2 gene is precisely control ed by the napin promoter. 展开更多
关键词 Transgenic rapeseed Real-time fluorescence quantitative PCR fad2gene Specific expression
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Cloning and Expression of Pun1 Gene Controlling Pungency of Pepper (Capsicum spp.) 被引量:1
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作者 董雨薇 孙樱燃 +5 位作者 毕然 孙宁莉 阮文渊 李越 王晶莹 郭庆勋 《Agricultural Science & Technology》 CAS 2016年第11期2483-2488,共6页
[Objective] This study was conducted to investigate cloning and expression of Pun1 gene controlling pungency of pepper. [Method] With Capsicum annuum L as a material, the cDNA sequence of Capunl gene was obtained, wit... [Objective] This study was conducted to investigate cloning and expression of Pun1 gene controlling pungency of pepper. [Method] With Capsicum annuum L as a material, the cDNA sequence of Capunl gene was obtained, with a total length of 1 457 bp, coding 440 amino acids. [Result] Phylogenetic analysis showed that Capunl was closest to Pun1 of C. chinense, with a genetic distance of 0.019 3. Plant expression vector pCAM-Punl-GFP was constructed and transformed into to- bacco, and it was found that the protein coded by fusion gene Punl::GFP was lo- cated on cell membrane. Prokaryotic expression vectors were constructed, and by SDS-PAGE and Western Blot detection, an induced protein with a molecular weight of 63 ku was obtained. It was found by real-time fluorescence quantitative expres- sion that Pun1 gene was expressed at the highest level 30 d after flowering, de- creased then, and could not be detected substantially 40 and 45 d after flowering. [Conclusion] This study provides information and reference for molecular regulation mechanism of Pun1 gene. 展开更多
关键词 PUNGENCY Pun1 gene Transient expression Prokaryotic expression
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Possible mechanism for hepatitis B virus X gene to induce apoptosis of hepatocytes 被引量:12
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作者 Sheng-Jun Zhang Hong-Ying Chen +1 位作者 Zhi-Xin Chen Xiao-Zhong Wang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第28期4351-4356,共6页
AIM: To investigate the possible mechanism for HBV X gene to induce apoptosis of hepatocyte HL-7702 cells.METHODS: HBV X gene eukaryon expression vector pcDNA3-X was established and transfected into HL-7702 cells by... AIM: To investigate the possible mechanism for HBV X gene to induce apoptosis of hepatocyte HL-7702 cells.METHODS: HBV X gene eukaryon expression vector pcDNA3-X was established and transfected into HL-7702 cells by lipid-mediated transfection, including transient and stable transfection. Positive clones were screened by incubating in the selective medium with 600 μg/mL G418 and named HL-7702/HBV-encoded X protein (HBx) cells. The expressions of Fas/FasL, Bax/Bcl-2, and c-myc mRNA were measured by semi-quantitative RT-PCR in HL-7702/HBx and control group, respectively.RESULTS: RT-PCR analysis confirmed that HBV X gene was transfected into HL-7702 cells successfully. By semiquantitative RT-PCR analysis, Bax and c-myc mRNA levels in HL-7702/HBx cells of transient transfection were significantly higher than those in control, FasL and c-myc mRNA levels in HL-7702/HBx cells of stable transfection were significantly higher than those in control, whereas the Bcl-2 mRNA levels in HL-7702/HBx cells of transient and stable transfection were significantly lower than thosein control.CONCLUSION: HBV X gene may promote the apoptosis of hepatocytes by regulating the expressions of Fas/FasL, Bax/Bcl-2, and c-myc gene in a dose-dependent manner. 展开更多
关键词 Hepatitis B virus X gene APOPTOSIS GENEEXPRESSION
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Adenovirus-mediated CTLA4Ig gene inhibits infiltration of immune cells and cell apoptosis in rats after liver transplantation 被引量:14
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作者 Guo-Ping Jiang, Zhen-Hua Hu, Shu-Sen Zheng, Chang-Ku Jia, Ai-Bin Zhang, Wei-Lin Wang, Department of Hepatobiliary Pancreatic Surgery, Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第7期1065-1069,共5页
AIM: To investigate the role of adenovirus-mediated CTLA4Ig gene therapy in inhibiting the infiltration of macrophages and CD8+T cells and cell apoptosis after liver transplantation. METHODS: The rat orthotopic liver ... AIM: To investigate the role of adenovirus-mediated CTLA4Ig gene therapy in inhibiting the infiltration of macrophages and CD8+T cells and cell apoptosis after liver transplantation. METHODS: The rat orthotopic liver transplantation model was applied. The rats were divided into three groups: group I: rejection control (SD-to-Wistar); group II: acute rejection treated with intramuscular injection of CsA 3.0 mg/(kg·d) for 12 d (SD-to-Wistar+CsA); groupIII: injection of 1×109 PFU adenovirus-mediated CTLA4Ig gene liquor in dorsal vein of penis 7 d before liver transplantation (SD-to-Wistar+CTLA4Ig). Immunohistochemistry and transferase-mediated dUTP nick-end labeling (TUNEL) were used to analyze the expression of CTLA4Ig gene in liver, infiltration of macrophages and CD8+T cells, cell apoptosis in grafts at different time-points after liver transplantation. Histopathological examination was done. RESULTS: CTLA4Ig gene expression was positive in liver on d 7 after administering adenovirus-mediated CTLA4Ig gene via vein, and remained positive until day 60 after liver transplantation. Infiltration of macrophages and CD8+T cells in CTLA4Ig-treated group was less than in rejection control group and CsA-treated group. The apoptotic index of rejection group on d 3, 5, and 7 were significantly higher than that of CTLA4Ig-treated group. A good correlation was found between severity of rejection reaction and infiltration of immune activator cells or cell apoptotic index in grafts. CONCLUSION: CTLA4Ig gene is constantly expressed in liver and plays an important role in inducing immune tolerance. 展开更多
关键词 Liver transplantation ADENOVIRUS CTLA4IG Apoptosis
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Association between Bmi1 and clinicopathological status of esophageal squamous cell carcinoma 被引量:15
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作者 Xiao-Ting He Xiu-Feng Cao +5 位作者 Lv Ji Bin Zhu Jin Lv Dong-Dong Wang Pei-Hua Lu Heng-Guan Cui 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第19期2389-2394,共6页
AIM: To investigate the clinicopathological roles of Bmil in esophageal squamous cell carcinoma (ESCC).METHODS: Quantitative real-time polymerase chain reaction and immunohistochemical staining for Broil were perf... AIM: To investigate the clinicopathological roles of Bmil in esophageal squamous cell carcinoma (ESCC).METHODS: Quantitative real-time polymerase chain reaction and immunohistochemical staining for Broil were performed in cancerous and adjacent non-cancerous paraffin-embedded esophageal specimens.RESULTS: The Bmil expression level was unaffected by gender and age. The level of Broil mRNA in ESCC was significantly higher than that in the adjacent non-cancerous tissues (2.181 ± 2.158 vs 0.931 ± 0.894, P = 0.0152), and its over-expression was aggressively associated with lymph node metastasis (3.580 ± 2.487 vs 1.703 ± 0.758, P = 0.0003), poorer cell differentiation (P = 0.0000) and advanced pathological stage (3.827± 2.673 vs 1.590 ± 0.735, P = 0.0001). The patients were divided into high-expression and low-expression groups based on the median expression level of Bmi1 mRNA, and a shorter overall survival time in the former group was observed. Immunohistochemistry for Bmi1 oncoprotein showed diffusely positive, focally positive and negative expression in 44, 16 and 10 of 70 ESCC cases, respectively, compared with three, two and five of 10 adjacent non-cancerous cases (P = 0.027). The positive rate of the oncoprotein in samples of histological grade Ⅲ was higher than that of grade Ⅱ(P = 0.031), but its expression had no relation to the lymph node metastasis and pathological staging. In 70 ESCC samples, Bmi1 showed high intense expression in the cytoplasm and less or even no expression in the nucleus.CONCLUSION: Bmi1 was over-expressed in ESCC. Increased Bmi1 mRNA expression was significantly associated with ESCC progression, and the oncoprotein was largely distributed in the cytoplasm of tumor cells. 展开更多
关键词 Esophageal squamous cell carcinoma Broil Quantitative real-time polymerase chain reaction Immu nohistochemistry CLINICOPATHOLOGY
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Analysis on Tissue-specific Expression of Dp XTH1 and Dp XTH2 Genes in Dahlia 被引量:1
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作者 张萍萍 王蕾 +4 位作者 陈驰 王利芬 郑必平 钱力鑫 谈建中 《Agricultural Science & Technology》 CAS 2015年第8期1596-1599,共4页
[Objective] This study aimed to investigate the functions and related mechanisms of xyloglucan Endotransglycosylase/hydrolases (XTHs) during the growth and development of dahlia. [Method] Using /3-actin as the refer... [Objective] This study aimed to investigate the functions and related mechanisms of xyloglucan Endotransglycosylase/hydrolases (XTHs) during the growth and development of dahlia. [Method] Using /3-actin as the reference gene, the rela- tive transcription levels of DpXTH1 and DpXTH2 genes in roots, stems, leaves and petals of dahlia were analyzed by real-time RT-PCR. [Result] The DpXTH1 and DpXTH2 were not expressed in the roots, but expressed abundantly in the petals of dahlia. There were little expressions in the stems and leaves of dahlia. [Conclusion] The DpXTH1 and DpXTH2 were petal-specific genes and closely related to the growth and development of petals in dahlia. 展开更多
关键词 DAHLIA Xyloglucan endotransglycosylase/hydrolases XTH gene Expres-sion specificity Real-time fluorescence quantitative PCR
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Expression of gamma-aminobutyric acid A receptor subunits aα_1,β_1,γ_2 mRNA in rats with hepatic encephalopathy 被引量:3
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作者 Xiao-QingLi LeiDong +1 位作者 Zhong-HuaLiu Jin-YanLuo 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第21期3319-3322,共4页
AIM To investigate the mRNA expression of gammaaminobutyric acid A (GABAA) receptor subunits α1,β1, γ2in different parts of the brain of rats with hepaticencephalopathy.METHODS: Twelve adult male Sprague-Dawley rat... AIM To investigate the mRNA expression of gammaaminobutyric acid A (GABAA) receptor subunits α1,β1, γ2in different parts of the brain of rats with hepaticencephalopathy.METHODS: Twelve adult male Sprague-Dawley rats were randomly divided into two groups: (1) hepatic encephalopathy model group (n = 6), which was induced by intraperitoneal injection of thioacetamide (TAA, 350 mg/kg) for threeconsecutive days; (2) control group (n = 6), in which the rats were treated with same dose of normal saline solution. After the freeze slice of cerebrum was made,in situ hybridization was used to detect the mRNA of GABAA receptor subunits α1, β1, and γ2 in rat cerebral cortex, basal nuclei, substantia nigra and hippocampi. Image data were collected and analyzed quantitatively by QWin550CWmodel image signal gather and analysis system. RESULTS: In rats with hepatic encephalopathy, mRNA expression levels of GABAA receptor subunits α1, β1 increased significantly in basal nuclei, substantia nigra pars compacta, substantia nigra pars reticularis and hippocampi (144.7±15.67/184.14±4.41, 60.61±33.66/113.07±32.44,87.71± 21.25/128.40±18.85, 122.34±5.56/161.60±4.56,123.29±5.21/140.65±4.15, 123.40±4.42/140.09±4.52,124.76±4.18/140.09±4.12, 141.62±15.09/182.80 ±5.20,69.13±30.74/134.21±43.76, 87.87±25.16/151.01±19.49,122.14±6.30/162.33±3.92, 122.81±5.09/137.19±7.12,123.00±4.63/138.11±5.92, 125.75 ±2.43/138.81±6.10,P<0.01), but did not change in the cerebral cortex compared to the control group. Similar changes were found in the mRNA expression levels of GABAA receptor subunit γ2,which increased significantly in basal nuclei, substantia nigra pars compacta, substantia nigra pars reticularis (136.81±26.41/167.97±16.23, 51.00±36.14/113.18±36.52, 86.35±20.30/126.90±19.74, P<0.01), CA1 of hippocampal (162.15±9.05/178.62±6.45, P<0.05), and no changes were found in the cerebral cortex and CA2, CA3, CA4 of hippocampi.CONCLUSION: In rats with hepatic encephalopathy, mRNA expression levels of GABAA receptor subunits α1,β1, γ2 increase significantly in basal nuclei, substantia nigra and hippocampi, suggesting that the changes of mRNA expression levels in GABAA receptor subunits may contribute to the pathogenesis of hepatic encephalopathy. 展开更多
关键词 Gama-aminobutyric acid A receptor MRNA
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Salt-responsive genes in rice revealed by cDNA microarray analysis 被引量:17
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作者 Dai Yin CHAO Yong Hai LUO +2 位作者 Min SHI Da LUO Hong Xuan LIN 《Cell Research》 SCIE CAS CSCD 2005年第10期796-810,共15页
We used cDNA microarrays containing ~9,000 unigenes to identify 486 salt responsive expressed sequence tags (ESTs) (representing ~450 unigenes) in shoots of the highly salt-tolerant rice variety, Nona Bokra (Oryza s... We used cDNA microarrays containing ~9,000 unigenes to identify 486 salt responsive expressed sequence tags (ESTs) (representing ~450 unigenes) in shoots of the highly salt-tolerant rice variety, Nona Bokra (Oryza sativa L. Ssp.Indica pv. Nona). Some of the genes identified in this study had previously been associated with salt stress. Howeverthe majority were novel, indicating that there is a great number of genes that are induced by salt exposure. Analysis of the salt stress expression profile data of Nona provided clues regarding some putative cellular and molecular processes that are undertaken by this tolerant rice variety in response to salt stress. Namely, we found that multiple transcription factors were induced during the initial salt response of shoots. Many genes whose encoded proteins are implicated in detoxification, protectant and transport were rapidly induced. Genes supporting photosynthesis were repressed and those supporting carbohydrate metabolism were altered. Commonality among the genes induced by salt exposure with those induced during senescence and biotic stress responses suggests that there are shared signaling pathways among these processes. We further compared the transcriptome changes of the salt-sensitive cultivar, IR28, with that of Nona rice. Many genes that are salt responsive in Nona were found to be differentially regulated in IR28. This study identified a large number of candidate functional genes that appear to be involved in salt tolerance and further examination of these genes may enable the molecular basis of salt tolerance to be elucidated. 展开更多
关键词 cDNA microarray RICE salt stress signaling crosstalk transcriptome.
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CHANGES OF ENDOTHELIN-1 GENE EXPRESSION IN RAT BRAINS DURING ISCHEMIA AND ISCHEMIC REPERFUSION  被引量:8
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作者 吴卫平 匡培根 李振洲 《Chinese Medical Sciences Journal》 CAS CSCD 1996年第4期228-231,共4页
Objective. The experiment was designed to study the association of cerebral ischemia and reperfusion with endothelin- 1 (ET- 1) gene expression of rat brains and time-dependent changes of ET- 1 gene expression during... Objective. The experiment was designed to study the association of cerebral ischemia and reperfusion with endothelin- 1 (ET- 1) gene expression of rat brains and time-dependent changes of ET- 1 gene expression during cerebral ischemia.Materials and methods. Thirty- three male SD rats were divided into dot blot hybridization(n = 27) and in silu hybridization groups(n= 6). The focal cerebral ischemia and reperfusion models were made with suture embolism of middle cerebral artery. Dot blot hybridization groups were redivided into control and ischemic subgroups (ischemia for 0. 5 , 1 , 1. 5 , 3 , 6 , 12 , 24 , 48 and 72 h respectively). In situ hybridization groups were redivided into ischemia and reperfusion groups. After 24 h ischemia and 24 h reperfusion,ET1 gene expressions were investigated with in situ hybridization and the resuhs were analyzed with IBAS 2000 Image Analysis System.Results. Dot blot hybridization showed that ET-1 mRNA of cerebral cortex and caudate- putamen was increased at 6 h of ischemia and reached peak at 24 h (3. 9 and 3. 7 fold respectively) ,and at 72 h of ischemia it remained at high levels(3. 5 and 2. 1 fold respectively). In silu hybridization showed that the levels of ET- 1 mRNA of cerebral cortex and caudate-putamen were also markedly increased both in 24 h ischemia and 24 h reperfusion groups (P<0. 01 , P<0. 05 respectively) .Conclusions. ET-1 gene expression in focal ischemic brain tissue were markedly and progressively increased during cerebral ischemia and reperfusion and downregulation of ET- 1 gene expression may be a new approach to the treatment of ischemic cerebrovascular diseases. 展开更多
关键词 endothelin- 1 gene expression cerebral ischemia
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Study on the Expression Laws and Regulatory Functions of miRNA-181b in the Anterior Pituitary of Cattle
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作者 许橙 薛文志 +8 位作者 赵茂鑫 陈信 刘颖 于汪洋 许艳丽 马腾壑 高妍 张嘉保 袁宝 《Agricultural Science & Technology》 CAS 2011年第7期1031-1034,共4页
[Objective] The research aimed to discuss the differential expression quantity of miRNA-181b in mature(18-month-old) and immature(one-month-old) cattle's anterior pituitary and its regulation function.[Method] cD... [Objective] The research aimed to discuss the differential expression quantity of miRNA-181b in mature(18-month-old) and immature(one-month-old) cattle's anterior pituitary and its regulation function.[Method] cDNA library of miRNA in mature(18-month-old) and immature(one-month-old) cattle's anterior pituitary were established.After Solexa high-throughput sequencing of miRNA in the cDNA library,miRNA in anterior pituitary of bulls was identified.miRNA-181b with differential expression were selected from the sequencing results.By real-time quantitative RT-PCR,the expression laws of miRNA-181b in the anterior pituitary of Yanbian Cattle in different growth period was validated.And the target genes of miRNA-181b were forecast by using TargetScanS prediction software.[Result] The expression quantity of miRNA-181b had great difference in cattle's anterior pituitary different growth periods.The expression quantity of miRNA-181b in anterior pituitary of one-month-old cattle was 4.05 times as that in 18-month-old cattle.The binding of miRNA-181b with 838-844 bases in 3' untranslated region of FSHβ gene was specific and the binding base sites were UGAAUGUA.[Conclusion] This research provided the theoretical basis for the transcription regulation research of FSHβ. 展开更多
关键词 MIRNA Real-time quantitative RT-PCR Differential expression
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Effect of non-Gaussian properties of the sea surface on the low-incidence radar backscatter and its inversion in terms of wave spectra by an ocean wave spectrometer 被引量:1
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作者 陈萍 尹巧华 黄萍 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2015年第5期1142-1156,共15页
The principle of ocean wave spectrometers was first presented several decades ago to detect the directional wave spectrum with real-aperture radar(Jackson,1981). To invert wave spectra using an ocean wave spectrometer... The principle of ocean wave spectrometers was first presented several decades ago to detect the directional wave spectrum with real-aperture radar(Jackson,1981). To invert wave spectra using an ocean wave spectrometer,for simplicity,the hydrodynamic forcing and wave-wave interaction effect are neglected and a Gaussian slope probability density function(pdf) is used to calculate the normalized backscattering cross-section( σ 0) of the ocean surface. However,the real sea surface is non-Gaussian. It is not known whether the non-Gaussian property of the sea surface will affect the performance of the inversion of the wave spectrum if following existing inversion steps and methods. In this paper,the pdf of the sea surface slope is expressed as a Gram-Charlier fourth-order expansion,which is quasi-Gaussian. The modulation transfer function(MTF) is derived for a non-Gaussian slope pdf. The effects of non-Gaussian properties of the sea surface slope on the inversion process and result are then studied in a simulation of the SWIM(Surface Waves Investigation and Monitoring) instrument configuration to be used on the CFOSAT(China-France Oceanography Satellite) mission. The simulation results show that the mean trend of σ 0 depends on the sea slope pdf,and the peakedness and skewness coefficients of the slope pdf affect the shape of the mean trend of σ 0 versus incidence and azimuth; owing to high resolution of σ 0 in the range direction,MTF obtained using the mean trend of σ 0 is almost as accurate as that set in the direct simulation; in the inversion,if ignoring the non-Gaussian assumption,the inversion performances for the wave spectrum decrease,as seen for an increase in the energy error of the inverted wave slope spectrum. However,the peak wavelength and wave direction are the same for inversions that consider and ignore the non-Gaussian property. 展开更多
关键词 slope probability density of the sea surface ocean wave spectrometer NON-GAUSSIAN directionalwave spectrum energy error
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Detection of Syndecan-1 and Heparanase-1 Genes in Esophageal Carcinoma by Quantitative RT-PCR 被引量:1
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作者 Jun-li SI Yu-qin QI +3 位作者 Jing-yuan CUI Song-mei WANG He WANG Mei LU 《Clinical oncology and cancer researeh》 CAS CSCD 2010年第4期253-258,共6页
OBJECTIVE To quantitatively explore the expression of Syndecan-1 and heparanase-1 in esophageal cancer tissue as well as their relationship with the clinicopathological factors, in order to evaluate their roles in tum... OBJECTIVE To quantitatively explore the expression of Syndecan-1 and heparanase-1 in esophageal cancer tissue as well as their relationship with the clinicopathological factors, in order to evaluate their roles in tumor invasion and metastasis.METHODS Real-time fluorescence quantitative PCR (Q-PCR) was used to analyze the expression levels of Syndecan-1 and heparanase-1 genes£?participants included 67 cases with esophageal cancers and 32 healthy volunteers.RESULTS The expression of Heparanase-1 gene in esophageal cancers was higher than that in normal esophageal tissue (P 〈 0.001), and the expression of Syndecan-1 gene in the normal esophageal tissue was higher compared with esophageal cancers (P 〈 0.001). The positive rates of Syndecan-1 and Heparanase-1 gene in esophageal cancer were 13.4% (9/67) and 85.1% (57/67).The expression of Syndecan-1 and Heparanase-1 genes was signifi cantly related to di. erentiation, depth of infi ltration, lymph node metastasis, vessel metastasis, and TNM stages of disease (P 〈 0.05). In an attempt to measure the association between the 2 agents, this study found that the expression of Syndecan-1 mRNA had a significantly negative correlation with the expression of Heparanase-1 mRNA by using Spearman rank correlation test (OR = -0.572, P 〈 0.001).CONCLUSION Syndecan-1 and Heparanase-1 play important roles in the invasion and metastasis of esophageal cancer. The reduction of Syndecan-1 and/or the increase of Heparanase-1 may influence the invasion and metastasis of malignant tumors.Thus the combination assay of Syndecan-1 and Heparanase-1 may contribute to the diagnosis and treatment of malignant tumors. 展开更多
关键词 SYNDECAN-1 esophageal neoplasms neoplasm invasiveness neoplasm metastasis PCR
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Expression of early growth response factor-1 in rats with cerulein-induced acute pancreatitis and its significance 被引量:4
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作者 Lan-Bo Gong Li He +2 位作者 Yang Liu Xue-Qing Chen Bo Jiang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第32期5022-5024,共3页
AIM: To observe the expressions of early growth response factor-1 (Egr-1) and tissue factor (TF) in rats with cerulein-induced acute pancreatitis and to explore its significance. METHODS: A large dose of cerulei... AIM: To observe the expressions of early growth response factor-1 (Egr-1) and tissue factor (TF) in rats with cerulein-induced acute pancreatitis and to explore its significance. METHODS: A large dose of cerulein was used to create the experimental acute pancreatitis model in rats. The changes of Egr-1 mRNA and protein in rats were observed during 30 min to 4 h after the treatment and immunohistochemical method was used to observe the localized expression of Egr-1 in tissues. In addition to the mRNA expression of Egr-1 target gene, TF was also observed. A blank control group, and a bombesinadministered group were used for comparison. RESULTS: Alter the stimulation of a large dose of cerulein, the rats showed typical inflammatory changes of acute pancreatitis. Thirty minutes alter the stimulation, the mRNA expression of Egr-1 in the pancreatic tissue reached its peak and then declined, while the expression of Egr-1 protein reached its peak 2 h after the stimulation. Histologically, 2 h after the stimulation, almost all pancreatic acinar cells had the expression of Egr-1 protein, which was focused in the nuclei. The mRNA expression of TF occurred 1 h after the stimulation and gradually increased within 4 h. However, a large dose of bombesin only stimulated the pancreatic tissue to produce a little mRNA expression of Egr-1 and no mRNA expression of Egr-1 protein and TF. CONCLUSION: Egr-1 as a pro-inflammatory transcription factor may play an important role in the pathogenesis of acute pancreatitis by modulating the expression of TF. 展开更多
关键词 Growth response factor-1 Tissue factor Acute pancreatitis CERULEIN BOMBESIN
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Clinical experimental study of Arnebia root oil promoting histological change and up-regulating bFGF expression in the wound 被引量:1
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作者 裴宪武 王坤正 +3 位作者 柏传毅 王强 姜志茹 高登峰 《Journal of Medical Colleges of PLA(China)》 CAS 2006年第1期55-59,共5页
Objective: To explore the molecular biological mechanism of Arnebia root oil promoting wound surface healing by observing histological change and bFGF expression in wound surface tissue as well as wound surface healin... Objective: To explore the molecular biological mechanism of Arnebia root oil promoting wound surface healing by observing histological change and bFGF expression in wound surface tissue as well as wound surface healing rate. Methods: Raw surface in patients were randomly divided into 2 groups. Experimental group was treated by Arnebia root oil and control group was treated by petrolatum gauze, then the tissular structure of raw surface was observed by histology, histochemistry. electron microscope and raw surface healing rates was compared either. bFGF expression in wound surface tissue was also evaluated by Western-blot. Results: Raw surface healing rate of experimental group and control group had obvious difference(P<0. 05). Raw surface of experimental group had more fibroblast, collagen and blood capillary. bFGF was expressed in both groups, and the level of bFGF expression in experimental group was higher than that in control group in every period. There were significant differences between 2 groups in gray-density value ( P<0. 05). Being as an internal control, no significant change was found for β-actin expression, although it occured in various phases. Conclusion: Arnebia root oil plays an important regulative role in the course of healing of wound and it can promote skin raw surface repair and accelerate wound surface healing, which are caused by enhancing bFGF in the wound tissue. 展开更多
关键词 arnebia root oil basic fibroblast growth factor raw surface histology WESTERN-BLOT
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THE ROLES OF bcl-2 GENE FAMILY IN THE PULMONARY ARTERY REMODELING OF HYPOXIA PULMONARY HYPERTENSION IN RATS 被引量:4
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作者 杨成 王胜发 +3 位作者 梁桃 王巨 王凯 王柏春 《Chinese Medical Sciences Journal》 CAS CSCD 2001年第3期182-184,共3页
Objective. To investigate the roles of apoptosis in the pulmonary artery remodeling of pulmonary hypertension secondary to hypoxia and illustrate the relative genes expression. Methods. Thirty rats were divided into h... Objective. To investigate the roles of apoptosis in the pulmonary artery remodeling of pulmonary hypertension secondary to hypoxia and illustrate the relative genes expression. Methods. Thirty rats were divided into hypoxia group( 10% O2, 8h/d) and normal control group. On the 15th day of hypoxia, pulmonary artery pressure and right ventricular hypertrophy index were measured and pulmonary artery vessels were studied by light microscope. Then terminal deoxynucleotidyl transferase- mediated dUTP nick- end labeling( TUNEL) technique was used to detect nucleosomal DNA fragmentation of apoptotic cells. In situ hybridization and RT- PCR were used to detect the expression level of bcl- 2 and bax. Results. The pulmonary artery pressure and right ventricular hypertrophy index of hypoxia group were increased significantly, the pulmonary artery wall of hypoxic group become incrassate than control group. Apoptotic cells can be found in lung with hypoxia or without hypoxia. Compared with control group, apoptotic index of hypoxic group decreased significantly. Through the methods of in situ hybridization and RT- PCR, we found the expression of bcl- 2 increased whereas bax decreased significantly in the hypoxic group. Conclusion. The alternation in bcl- 2 and bax expression induced by hypoxia play an important role in the pulmonary artery remodeling which is the main pathologic change of pulmonary hypertension secondary to hypoxia. 展开更多
关键词 pulmonary hypertension pulmonary artery remodeling bcl- 2
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